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Protein

Envelope glycoprotein E

Gene

gE

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.4 Publications
The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein E
Short name:
gE
Alternative name(s):
gE-1
Gene namesi
Name:gE
ORF Names:US8
OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10304 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 421Virion surfaceSequence analysisAdd BLAST401
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 552IntravirionSequence analysisAdd BLAST110

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell junction, Host cell membrane, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003822621 – 552Envelope glycoprotein EAdd BLAST532

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi124N-linked (GlcNAc...); by hostSequence analysis1
Modified residuei176SulfotyrosineSequence analysis1
Glycosylationi245N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi273 ↔ 299By similarity
Disulfide bondi282 ↔ 291By similarity
Disulfide bondi316 ↔ 325By similarity
Modified residuei478Phosphoserine; by host CK2By similarity1
Modified residuei479Phosphoserine; by host CK2By similarity1
Modified residuei505PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.Curated
N-glycosylated, and sulfated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Interactioni

Subunit structurei

Interacts with gI; this interaction enhances the Fc receptor function of gE. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 88Interaction with gIBy similarityAdd BLAST26
Regioni237 – 382Fc-bindingBy similarityAdd BLAST146
Regioni472 – 497Interaction with VP22 and UL11By similarityAdd BLAST26
Regioni478 – 486AcidicBy similarity9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi465 – 468Internalization motifSequence analysis4
Motifi474 – 477Internalization motifSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi163 – 216Pro-richAdd BLAST54

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003404. Herpes_glycopE.
IPR007110. Ig-like_dom.
[Graphical view]
PfamiPF02480. Herpes_gE. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q703F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRGAVVGFL LGVCVVSCLA GTPKTSWRRV SVGEDVSLLP APGPTGRGPT
60 70 80 90 100
QKLLWAVEPL DGCGPLHPSW XSLMPPKQVP ETVVDAACMR APVPLAMAYA
110 120 130 140 150
PPAPSATGGL RTDFVWQERA AVVNRSLVIY GVRETDSGLY TLSVGDIKDP
160 170 180 190 200
ARQVASVVLV VQPAPVPTPP PTPADYDEDD NDEGEGEDES LAGTPASGTP
210 220 230 240 250
RLPPPPAPPR SWPSAPEVSH VRGVTVRMET PEAILFSPGE AFSTNVSIHA
260 270 280 290 300
IAHDDQTYTM DVVWLRFDVP TSCAEMRIYE SCLYHPQLPE CLSPADAPCA
310 320 330 340 350
ASTWTSRLAV RSYAGCSRTN PPPRCSAEAH MEPVPGLAWQ AASVNLEFRD
360 370 380 390 400
ASPQHSGLYL CVVYVNDHIH AWGHITISTA AXYRNAVVEQ PLPQRGADLA
410 420 430 440 450
EPTHPHVGAP PHAPPTHGAL RLGAVMGAAL LLSVLGLSVW ACMTCWRRRA
460 470 480 490 500
WRAVKSRASG KGPTYIRVAD SELYADWSSD SEGERDQVPW LAPPERPDSP
510 520 530 540 550
STNGSGFEIL SPTAPSVYPR SDGHQSRRQL TTFGSGRPDR RYSQASDSSV

FW
Length:552
Mass (Da):59,313
Last modified:July 5, 2004 - v1
Checksum:i8F689BF77BE07BB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ626469 Genomic DNA. Translation: CAF24756.1.
S62895 Genomic DNA. Translation: AAB27080.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ626469 Genomic DNA. Translation: CAF24756.1.
S62895 Genomic DNA. Translation: AAB27080.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR003404. Herpes_glycopE.
IPR007110. Ig-like_dom.
[Graphical view]
PfamiPF02480. Herpes_gE. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGE_HHV1F
AccessioniPrimary (citable) accession number: Q703F0
Secondary accession number(s): Q86624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: March 16, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.