Q703F0 (GE_HHV1F) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 46. History...
Names and origin
|Protein names||Recommended name:|
Envelope glycoprotein E
|Organism||Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)|
|Taxonomic identifier||10304 [NCBI]|
|Taxonomic lineage||Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||552 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips. Ref.3 Ref.4 Ref.5 Ref.6
The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses By similarity. Ref.3 Ref.4 Ref.5 Ref.6
Interacts with gI; this interaction enhances the Fc receptor function of gE. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein By similarity. Ref.3
Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein. Host endosome membrane; Single-pass membrane protein Probable. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection. Ref.4 Ref.5 Ref.7
Phosphorylated on serines within the acidic cluster Potential. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane Potential.
N-glycosylated, and sulfated By similarity.
Belongs to the alphaherpesvirinae glycoprotein E family.
|Biological process||Host-virus interaction|
|Cellular component||Host cell junction|
Host cell membrane
Host Golgi apparatus
Viral envelope protein
|Gene Ontology (GO)|
|Biological_process||evasion or tolerance by virus of host immune response|
Inferred from electronic annotation. Source: UniProtKB-KW
|Cellular_component||host cell Golgi membrane|
Inferred from electronic annotation. Source: UniProtKB-SubCellhost cell endosome membrane
Inferred from electronic annotation. Source: UniProtKB-SubCellhost cell junction
Inferred from electronic annotation. Source: UniProtKB-SubCellintegral component of membrane
Inferred from electronic annotation. Source: UniProtKB-KWviral envelope
Inferred from electronic annotation. Source: UniProtKB-KWvirion membrane
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Signal peptide||1 – 20||20||Potential|
|Chain||21 – 552||532||Envelope glycoprotein E||PRO_0000038226|
|Topological domain||21 – 421||401||Virion surface Potential|
|Transmembrane||422 – 442||21||Helical; Potential|
|Topological domain||443 – 552||110||Intravirion Potential|
|Region||63 – 88||26||Interaction with gI By similarity|
|Region||237 – 382||146||Fc-binding By similarity|
|Region||472 – 497||26||Interaction with VP22 and UL11 By similarity|
|Region||478 – 486||9||Acidic By similarity|
|Motif||465 – 468||4||Internalization motif Potential|
|Motif||474 – 477||4||Internalization motif Potential|
|Compositional bias||163 – 216||54||Pro-rich|
Amino acid modifications
|Modified residue||176||1||Sulfotyrosine Potential|
|Modified residue||478||1||Phosphoserine; by host CK2 By similarity|
|Modified residue||479||1||Phosphoserine; by host CK2 By similarity|
|Modified residue||505||1||Phosphoserine By similarity|
|Glycosylation||124||1||N-linked (GlcNAc...); by host Potential|
|Glycosylation||245||1||N-linked (GlcNAc...); by host Potential|
|Disulfide bond||273 ↔ 299||By similarity|
|Disulfide bond||282 ↔ 291||By similarity|
|Disulfide bond||316 ↔ 325||By similarity|
|||"Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses."|
Norberg P.R., Bergstroem T., Kekabdar E., Lindh M., Liljeqvist J.-A.
J. Virol. 78:10755-10764(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
|||"Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome."|
Georgopoulou U., Michaelidou A., Roizman B., Mavromara-Nazos P.
J. Virol. 67:3961-3968(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-552.
|||"Herpes simplex virus immunoglobulin G Fc receptor activity depends on a complex of two viral glycoproteins, gE and gI."|
Johnson D.C., Frame M.C., Ligas M.W., Cross A.M., Stow N.D.
J. Virol. 62:1347-1354(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GLYCOPROTEIN I.
|||"Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread."|
Johnson D.C., Webb M., Wisner T.W., Brunetti C.
J. Virol. 75:821-833(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
|||"Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread."|
Farnsworth A., Johnson D.C.
J. Virol. 80:3167-3179(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
|||"Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons."|
Snyder A., Polcicova K., Johnson D.C.
J. Virol. 82:10613-10624(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"Comprehensive characterization of extracellular herpes simplex virus type 1 virions."|
Loret S., Guay G., Lippe R.
J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
|AJ626469 Genomic DNA. Translation: CAF24756.1.|
S62895 Genomic DNA. Translation: AAB27080.1.
3D structure databases
Protocols and materials databases
Family and domain databases
|InterPro||IPR003404. Herpes_glycopE. |
|Pfam||PF02480. Herpes_gE. 1 hit. |
|Accession||Primary (citable) accession number: Q703F0|
Secondary accession number(s): Q86624
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families