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Q703F0

- GE_HHV1F

UniProt

Q703F0 - GE_HHV1F

Protein

Envelope glycoprotein E

Gene

gE

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.4 Publications
    The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses By similarity.By similarity

    GO - Biological processi

    1. evasion or tolerance by virus of host immune response Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein E
    Short name:
    gE
    Alternative name(s):
    gE-1
    Gene namesi
    Name:gE
    ORF Names:US8
    OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10304 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Virion membrane; Single-pass type I membrane protein. Host cell membrane By similarity; Single-pass type I membrane protein By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein. Host endosome membrane Curated; Single-pass membrane protein Curated
    Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection.

    GO - Cellular componenti

    1. host cell endosome membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. host cell junction Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. viral envelope Source: UniProtKB-KW
    6. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell junction, Host cell membrane, Host endosome, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 552532Envelope glycoprotein EPRO_0000038226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi124 – 1241N-linked (GlcNAc...); by hostSequence Analysis
    Modified residuei176 – 1761SulfotyrosineSequence Analysis
    Glycosylationi245 – 2451N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi273 ↔ 299By similarity
    Disulfide bondi282 ↔ 291By similarity
    Disulfide bondi316 ↔ 325By similarity
    Modified residuei478 – 4781Phosphoserine; by host CK2By similarity
    Modified residuei479 – 4791Phosphoserine; by host CK2By similarity
    Modified residuei505 – 5051PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.Curated
    N-glycosylated, and sulfated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

    Interactioni

    Subunit structurei

    Interacts with gI; this interaction enhances the Fc receptor function of gE. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein By similarity.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 421401Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini443 – 552110IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei422 – 44221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 8826Interaction with gIBy similarityAdd
    BLAST
    Regioni237 – 382146Fc-bindingBy similarityAdd
    BLAST
    Regioni472 – 49726Interaction with VP22 and UL11By similarityAdd
    BLAST
    Regioni478 – 4869AcidicBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi465 – 4684Internalization motifSequence Analysis
    Motifi474 – 4774Internalization motifSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi163 – 21654Pro-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR003404. Herpes_glycopE.
    [Graphical view]
    PfamiPF02480. Herpes_gE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q703F0-1 [UniParc]FASTAAdd to Basket

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    MDRGAVVGFL LGVCVVSCLA GTPKTSWRRV SVGEDVSLLP APGPTGRGPT    50
    QKLLWAVEPL DGCGPLHPSW XSLMPPKQVP ETVVDAACMR APVPLAMAYA 100
    PPAPSATGGL RTDFVWQERA AVVNRSLVIY GVRETDSGLY TLSVGDIKDP 150
    ARQVASVVLV VQPAPVPTPP PTPADYDEDD NDEGEGEDES LAGTPASGTP 200
    RLPPPPAPPR SWPSAPEVSH VRGVTVRMET PEAILFSPGE AFSTNVSIHA 250
    IAHDDQTYTM DVVWLRFDVP TSCAEMRIYE SCLYHPQLPE CLSPADAPCA 300
    ASTWTSRLAV RSYAGCSRTN PPPRCSAEAH MEPVPGLAWQ AASVNLEFRD 350
    ASPQHSGLYL CVVYVNDHIH AWGHITISTA AXYRNAVVEQ PLPQRGADLA 400
    EPTHPHVGAP PHAPPTHGAL RLGAVMGAAL LLSVLGLSVW ACMTCWRRRA 450
    WRAVKSRASG KGPTYIRVAD SELYADWSSD SEGERDQVPW LAPPERPDSP 500
    STNGSGFEIL SPTAPSVYPR SDGHQSRRQL TTFGSGRPDR RYSQASDSSV 550
    FW 552
    Length:552
    Mass (Da):59,313
    Last modified:July 5, 2004 - v1
    Checksum:i8F689BF77BE07BB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ626469 Genomic DNA. Translation: CAF24756.1.
    S62895 Genomic DNA. Translation: AAB27080.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ626469 Genomic DNA. Translation: CAF24756.1 .
    S62895 Genomic DNA. Translation: AAB27080.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR003404. Herpes_glycopE.
    [Graphical view ]
    Pfami PF02480. Herpes_gE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses."
      Norberg P.R., Bergstroem T., Kekabdar E., Lindh M., Liljeqvist J.-A.
      J. Virol. 78:10755-10764(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome."
      Georgopoulou U., Michaelidou A., Roizman B., Mavromara-Nazos P.
      J. Virol. 67:3961-3968(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-552.
    3. "Herpes simplex virus immunoglobulin G Fc receptor activity depends on a complex of two viral glycoproteins, gE and gI."
      Johnson D.C., Frame M.C., Ligas M.W., Cross A.M., Stow N.D.
      J. Virol. 62:1347-1354(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GLYCOPROTEIN I.
    4. "Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread."
      Johnson D.C., Webb M., Wisner T.W., Brunetti C.
      J. Virol. 75:821-833(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread."
      Farnsworth A., Johnson D.C.
      J. Virol. 80:3167-3179(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons."
      Snyder A., Polcicova K., Johnson D.C.
      J. Virol. 82:10613-10624(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
      Loret S., Guay G., Lippe R.
      J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGE_HHV1F
    AccessioniPrimary (citable) accession number: Q703F0
    Secondary accession number(s): Q86624
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3