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Q703F0 (GE_HHV1F) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Envelope glycoprotein E

Short name=gE
Alternative name(s):
gE-1
Gene names
Name:gE
ORF Names:US8
OrganismHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifier10304 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips. Ref.3 Ref.4 Ref.5 Ref.6

The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses By similarity. Ref.3 Ref.4 Ref.5 Ref.6

Subunit structure

Interacts with gI; this interaction enhances the Fc receptor function of gE. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein By similarity. Ref.3

Subcellular location

Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein. Host endosome membrane; Single-pass membrane protein Probable. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection. Ref.4 Ref.5 Ref.7

Post-translational modification

Phosphorylated on serines within the acidic cluster Potential. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane Potential.

N-glycosylated, and sulfated By similarity.

Sequence similarities

Belongs to the alphaherpesvirinae glycoprotein E family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 552532Envelope glycoprotein E
PRO_0000038226

Regions

Topological domain21 – 421401Virion surface Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 552110Intravirion Potential
Region63 – 8826Interaction with gI By similarity
Region237 – 382146Fc-binding By similarity
Region472 – 49726Interaction with VP22 and UL11 By similarity
Region478 – 4869Acidic By similarity
Motif465 – 4684Internalization motif Potential
Motif474 – 4774Internalization motif Potential
Compositional bias163 – 21654Pro-rich

Amino acid modifications

Modified residue1761Sulfotyrosine Potential
Modified residue4781Phosphoserine; by host CK2 By similarity
Modified residue4791Phosphoserine; by host CK2 By similarity
Modified residue5051Phosphoserine By similarity
Glycosylation1241N-linked (GlcNAc...); by host Potential
Glycosylation2451N-linked (GlcNAc...); by host Potential
Disulfide bond273 ↔ 299 By similarity
Disulfide bond282 ↔ 291 By similarity
Disulfide bond316 ↔ 325 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q703F0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8F689BF77BE07BB7

FASTA55259,313
        10         20         30         40         50         60 
MDRGAVVGFL LGVCVVSCLA GTPKTSWRRV SVGEDVSLLP APGPTGRGPT QKLLWAVEPL 

        70         80         90        100        110        120 
DGCGPLHPSW XSLMPPKQVP ETVVDAACMR APVPLAMAYA PPAPSATGGL RTDFVWQERA 

       130        140        150        160        170        180 
AVVNRSLVIY GVRETDSGLY TLSVGDIKDP ARQVASVVLV VQPAPVPTPP PTPADYDEDD 

       190        200        210        220        230        240 
NDEGEGEDES LAGTPASGTP RLPPPPAPPR SWPSAPEVSH VRGVTVRMET PEAILFSPGE 

       250        260        270        280        290        300 
AFSTNVSIHA IAHDDQTYTM DVVWLRFDVP TSCAEMRIYE SCLYHPQLPE CLSPADAPCA 

       310        320        330        340        350        360 
ASTWTSRLAV RSYAGCSRTN PPPRCSAEAH MEPVPGLAWQ AASVNLEFRD ASPQHSGLYL 

       370        380        390        400        410        420 
CVVYVNDHIH AWGHITISTA AXYRNAVVEQ PLPQRGADLA EPTHPHVGAP PHAPPTHGAL 

       430        440        450        460        470        480 
RLGAVMGAAL LLSVLGLSVW ACMTCWRRRA WRAVKSRASG KGPTYIRVAD SELYADWSSD 

       490        500        510        520        530        540 
SEGERDQVPW LAPPERPDSP STNGSGFEIL SPTAPSVYPR SDGHQSRRQL TTFGSGRPDR 

       550 
RYSQASDSSV FW 

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References

[1]"Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses."
Norberg P.R., Bergstroem T., Kekabdar E., Lindh M., Liljeqvist J.-A.
J. Virol. 78:10755-10764(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome."
Georgopoulou U., Michaelidou A., Roizman B., Mavromara-Nazos P.
J. Virol. 67:3961-3968(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-552.
[3]"Herpes simplex virus immunoglobulin G Fc receptor activity depends on a complex of two viral glycoproteins, gE and gI."
Johnson D.C., Frame M.C., Ligas M.W., Cross A.M., Stow N.D.
J. Virol. 62:1347-1354(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GLYCOPROTEIN I.
[4]"Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread."
Johnson D.C., Webb M., Wisner T.W., Brunetti C.
J. Virol. 75:821-833(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread."
Farnsworth A., Johnson D.C.
J. Virol. 80:3167-3179(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons."
Snyder A., Polcicova K., Johnson D.C.
J. Virol. 82:10613-10624(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
Loret S., Guay G., Lippe R.
J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ626469 Genomic DNA. Translation: CAF24756.1.
S62895 Genomic DNA. Translation: AAB27080.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003404. Herpes_glycopE.
[Graphical view]
PfamPF02480. Herpes_gE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGE_HHV1F
AccessionPrimary (citable) accession number: Q703F0
Secondary accession number(s): Q86624
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families