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Q703F0

- GE_HHV1F

UniProt

Q703F0 - GE_HHV1F

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Protein

Envelope glycoprotein E

Gene

gE

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.4 Publications
The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses By similarity.By similarity

GO - Biological processi

  1. evasion or tolerance by virus of host immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein E
Short name:
gE
Alternative name(s):
gE-1
Gene namesi
Name:gE
ORF Names:US8
OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10304 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein. Host cell membrane By similarity; Single-pass type I membrane protein By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein. Host endosome membrane Curated; Single-pass membrane protein Curated
Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection.

GO - Cellular componenti

  1. host cell endosome Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell junction Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell junction, Host cell membrane, Host endosome, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 552532Envelope glycoprotein EPRO_0000038226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...); by hostSequence Analysis
Modified residuei176 – 1761SulfotyrosineSequence Analysis
Glycosylationi245 – 2451N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi273 ↔ 299By similarity
Disulfide bondi282 ↔ 291By similarity
Disulfide bondi316 ↔ 325By similarity
Modified residuei478 – 4781Phosphoserine; by host CK2By similarity
Modified residuei479 – 4791Phosphoserine; by host CK2By similarity
Modified residuei505 – 5051PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.Curated
N-glycosylated, and sulfated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Interactioni

Subunit structurei

Interacts with gI; this interaction enhances the Fc receptor function of gE. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein By similarity.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 421401Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini443 – 552110IntravirionSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei422 – 44221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 8826Interaction with gIBy similarityAdd
BLAST
Regioni237 – 382146Fc-bindingBy similarityAdd
BLAST
Regioni472 – 49726Interaction with VP22 and UL11By similarityAdd
BLAST
Regioni478 – 4869AcidicBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi465 – 4684Internalization motifSequence Analysis
Motifi474 – 4774Internalization motifSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 21654Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003404. Herpes_glycopE.
[Graphical view]
PfamiPF02480. Herpes_gE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q703F0-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDRGAVVGFL LGVCVVSCLA GTPKTSWRRV SVGEDVSLLP APGPTGRGPT
60 70 80 90 100
QKLLWAVEPL DGCGPLHPSW XSLMPPKQVP ETVVDAACMR APVPLAMAYA
110 120 130 140 150
PPAPSATGGL RTDFVWQERA AVVNRSLVIY GVRETDSGLY TLSVGDIKDP
160 170 180 190 200
ARQVASVVLV VQPAPVPTPP PTPADYDEDD NDEGEGEDES LAGTPASGTP
210 220 230 240 250
RLPPPPAPPR SWPSAPEVSH VRGVTVRMET PEAILFSPGE AFSTNVSIHA
260 270 280 290 300
IAHDDQTYTM DVVWLRFDVP TSCAEMRIYE SCLYHPQLPE CLSPADAPCA
310 320 330 340 350
ASTWTSRLAV RSYAGCSRTN PPPRCSAEAH MEPVPGLAWQ AASVNLEFRD
360 370 380 390 400
ASPQHSGLYL CVVYVNDHIH AWGHITISTA AXYRNAVVEQ PLPQRGADLA
410 420 430 440 450
EPTHPHVGAP PHAPPTHGAL RLGAVMGAAL LLSVLGLSVW ACMTCWRRRA
460 470 480 490 500
WRAVKSRASG KGPTYIRVAD SELYADWSSD SEGERDQVPW LAPPERPDSP
510 520 530 540 550
STNGSGFEIL SPTAPSVYPR SDGHQSRRQL TTFGSGRPDR RYSQASDSSV

FW
Length:552
Mass (Da):59,313
Last modified:July 5, 2004 - v1
Checksum:i8F689BF77BE07BB7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ626469 Genomic DNA. Translation: CAF24756.1.
S62895 Genomic DNA. Translation: AAB27080.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ626469 Genomic DNA. Translation: CAF24756.1 .
S62895 Genomic DNA. Translation: AAB27080.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR003404. Herpes_glycopE.
[Graphical view ]
Pfami PF02480. Herpes_gE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses."
    Norberg P.R., Bergstroem T., Kekabdar E., Lindh M., Liljeqvist J.-A.
    J. Virol. 78:10755-10764(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome."
    Georgopoulou U., Michaelidou A., Roizman B., Mavromara-Nazos P.
    J. Virol. 67:3961-3968(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-552.
  3. "Herpes simplex virus immunoglobulin G Fc receptor activity depends on a complex of two viral glycoproteins, gE and gI."
    Johnson D.C., Frame M.C., Ligas M.W., Cross A.M., Stow N.D.
    J. Virol. 62:1347-1354(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLYCOPROTEIN I.
  4. "Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread."
    Johnson D.C., Webb M., Wisner T.W., Brunetti C.
    J. Virol. 75:821-833(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread."
    Farnsworth A., Johnson D.C.
    J. Virol. 80:3167-3179(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons."
    Snyder A., Polcicova K., Johnson D.C.
    J. Virol. 82:10613-10624(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
    Loret S., Guay G., Lippe R.
    J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGE_HHV1F
AccessioniPrimary (citable) accession number: Q703F0
Secondary accession number(s): Q86624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3