Reviewed, UniProtKB/Swiss-Prot Q701R3 (SIAT2_RAT)

Names and origin

Protein names

Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 2
      Short name=Alpha 2,6-ST 2
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
    Sialyltransferase 2
    ST6Gal II
      Short name=ST6GalII

Gene names

Name:

St6gal2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids By similarity.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	oligosaccharide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Beta-galactoside alpha-2,6-sialyltransferase 2		PRO_0000314788
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Signal-anchor for type II membrane protein Potential
	Topological domain	33 – 525	493	Lumenal Potential
Amino acid modifications
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	521	1	N-linked (GlcNAc...) Potential
	Disulfide bond	292 ↔ 444		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q701R3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E68DE58A6F73634F	FASTA	525	59,917
	10 20 30 40 50 60 MKPHLKQWRQ RMLFAIFVWG LLFLAIFIYF TNSNPAAPMP SSFSFLESRG LLPVQGKQRV 70 80 90 100 110 120 IMGALQEPSL PRSLEPSKVL MDGHSASPFN SWPGDPQKGD QAQDGFDNGD EFFTSQVGRK 130 140 150 160 170 180 SQSAFYPEED NYFFVAGQPG LYHHRQGALG LPSPGESSWQ SGPGQPKQEK LRHPRRGSLP 190 200 210 220 230 240 EEAYDSDMLS TSMSRAFLYR LWKGTVSSKM LNPRLQKAMR YYMSFNKHGV RFSRRGRREA 250 260 270 280 290 300 RRTGPELLCE MRKRVRVRTL DGKEAPFSGL GWRPLVPGVP LSQLHPRGLR SCAVVMSAGA 310 320 330 340 350 360 ILNSSLGEEI DSHDAVLRFN SAPTRGYEKD VGNKTTVRII NSQILANPSH HFIDSSLYKD 370 380 390 400 410 420 VILVAWDPAP YSANLNLWYK KPDYNLFTPY IQHRLKYPTQ PFYILHPKFI WQLWDIIQEN 430 440 450 460 470 480 TREKIQPNPP SSGFIGILVM MSMCQEVHVY EYIPSVRQTE LCHYHELYYD AACTLGAYHP 490 500 510 520 LLYEKLLVQR LNTGTQADLH HKGKVVLPGF QTLRCPVTRP NNTNT

References

[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ627626 mRNA. Translation: CAF29494.1.
IPI	IPI00371538.
RefSeq	XP_001058146.1. XP_236826.2.
UniGene	Rn.214119
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
GeneID	301155.
KEGG	rno:301155.
Organism-specific databases
CTD	301155.
RGD	1559663. St6gal2.
Phylogenomic databases
HOVERGEN	Q701R3.
Enzyme and pathway databases
BRENDA	2.4.99.1. 248.
Gene expression databases
Genevestigator	Q701R3.
Family and domain databases
InterPro	IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR001675. Glyco_trans_29. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	648263.

Entry information

Entry name

SIAT2_RAT

Accession

Primary (citable) accession number: Q701R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)