Reviewed, UniProtKB/Swiss-Prot Q701R2 (SIAT2_DANRE)

Names and origin

Protein names

Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 2
      Short name=Alpha 2,6-ST 2
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
    Sialyltransferase 2
    ST6Gal II
      Short name=ST6GalII

Gene names

Name:

st6gal2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates By similarity.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Beta-galactoside alpha-2,6-sialyltransferase 2		PRO_0000314790
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 514	483	Lumenal Potential
Amino acid modifications
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Disulfide bond	289 ↔ 441		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q701R2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 934DDA4D1E74B0B9	FASTA	514	58,533
	10 20 30 40 50 60 MKSSLKQWRR LALGLILVWA LLFLALLSYF MESRVDDPHA AAALSYTDTR RLTSLQGNPR 70 80 90 100 110 120 TIMATHLGLA TSSAPSTSSN TQQEQSQEEN PSADPQPSPL SQEAYPYPDP QSLAAWSAFG 130 140 150 160 170 180 TQDVGSRSTG VSRNRERQEY NQDSPQEDED EEEEVIGGEE EDEEGGDEGR GRTTKRVARH 190 200 210 220 230 240 GSSDPHEYYV PRYKSIVHGL WKGSLSMGML SPRLQRAMKD YLNNNKHGVA YRGHRKAKQS 250 260 270 280 290 300 RQQVLCELKK REKIRTLDGA EMPFSKLGWQ KIVPALPLSQ IHRPGLKTCA VVTSAGAMLH 310 320 330 340 350 360 SGLGKEIDSH DAVLRFNTAP TVGYERDVGN KTTIRIINSQ ILANPMHRFN RSSLYKNVTL 370 380 390 400 410 420 VAWDPAPYTL NLHQWYSNPD YNLFTPYMEY RMRFPSQPFY ILHPKYIWQL WDVIQANNLE 430 440 450 460 470 480 NIQPNPPSSG FIGILLMMSL CEEVHVYEYI PSLRQTDLCH YHERYYDAAC TLGAYHPLLY 490 500 510 EKMLIQRMNI GSEDELKRKG KVTLPGFNKV HCEP

References

[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Phylogeny of sialyltransferases." Lehmann F. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ627627 mRNA. Translation: CAF29495.1. AJ705078 mRNA. Translation: CAG29200.1.
IPI	IPI00486006.
UniGene	Dr.82299
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
Ensembl	ENSDART00000057484; ENSDARP00000057483; ENSDARG00000039335; Danio rerio. [Genome view]
Organism-specific databases
ZFIN	ZDB-GENE-060322-4. st6gal2.
Phylogenomic databases
eggNOG	fiNOG08912.
HOGENOM	HBG717863.
HOVERGEN	Q701R2.
InParanoid	Q701R2.
Enzyme and pathway databases
BRENDA	2.4.99.1. 96826.
Gene expression databases
ArrayExpress	Q701R2.
Bgee	Q701R2.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIAT2_DANRE

Accession

Primary (citable) accession number: Q701R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project