Reviewed, UniProtKB/Swiss-Prot Q701P2 (KO1_GIBIN)

Names and origin

Protein names

Recommended name:
    Ent-kaurene oxidase
    EC=1.14.13.78
Alternative name(s):
    Cytochrome P450-4
    Cytochrome P450 503A1

Gene names

Name:

CYP503A1

Organism

Gibberella intermedia (Bulb rot disease fungus) (Fusarium proliferatum)

Taxonomic identifier

42674 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella › Gibberella fujikuroi complex

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid, a key step in gibberellin (GA) biosynthesis.
Catalytic activity	Ent-kaur-16-ene + NADPH + O(2) = ent-kaur-16-en-19-ol + NADP(+) + H(2)O. Ent-kaur-16-en-19-ol + NADPH + O(2) = ent-kaur-16-en-19-al + NADP(+) + 2 H(2)O. Ent-kaur-16-en-19-al + NADPH + O(2) = ent-kaur-16-en-19-oate + NADP(+) + H(2)O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Miscellaneous	This protein is poorly expressed and has no detectable ent-kaurene oxidase activity in this species. Consequently, this species does not produce gibberellins.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ent-kaurene oxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Ent-kaurene oxidase		PRO_0000052209
Regions
	Transmembrane	31 – 51	21	Potential
Sites
	Metal binding	466	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q701P2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4E05AE3CEC4D3451	FASTA	525	59,974
	10 20 30 40 50 60 MNKSNSMNNT SLERLFQQLV LGLDGIPLMD VHWLIYVAFG AWLCSYVIHV LSSSSTVKVP 70 80 90 100 110 120 VVGYRSVFEP TWLLRLRFVW EGGSIIGQGY NKFKDSIFQV RKLGTDIVII PPNFIDEVRK 130 140 150 160 170 180 LSQDKTRSVE PFINDFAGQY TRGMVFLQSD LQNRVIQQRL TPKLVSLTKV MKEELDYALT 190 200 210 220 230 240 KEIPDMKDDE WVEVDISSIM VRLISRISAR VFLGPEHCRN QEWLTNTAEY SESLFITGFI 250 260 270 280 290 300 LRVVPHILRP FIAPLLPSYR TLLRNVSSGR RVIGDIIRSQ QGDGNEDILS WMRDAATGEE 310 320 330 340 350 360 KQIDNIAQRM LILSLASIHT TAMTMTHAMY DLCARPEYIE PLRDEVKGVV DASGWDKTAL 370 380 390 400 410 420 NRLHRLDSFL KESQRFNPVF LLTFNRIYHQ SMTLSDGTNL PSGTRIAVPS HAMLQDSAHV 430 440 450 460 470 480 PGPTPPTEFD GFRYSKIRSD SNYAQKYLFS MTDSSNMAFG YGKYACPGRF YASNEMKLTL 490 500 510 520 AILLLQFEFK LPDGKGRPRN ITIDSDMIPD PRARLCVRKR SLRDE

References

[1]	"Functional characterization of two cytochrome P450 monooxygenase genes, P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium proliferatum (Gibberella fujikuroi MP-D)." Malonek S., Rojas M.C., Hedden P., Gaskin P., Hopkins P., Tudzynski B. Appl. Environ. Microbiol. 71:1462-1472(2005) [PubMed: 15746349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D02945.
[2]	"Restoration of gibberellin production in Fusarium proliferatum by functional complementation of enzymatic blocks." Malonek S., Rojas M.C., Hedden P., Hopkins P., Tudzynski B. Appl. Environ. Microbiol. 71:6014-6025(2005) [PubMed: 16204516] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ628021 Genomic DNA. Translation: CAF31352.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
ProtoNet	Search...

Entry information

Entry name

KO1_GIBIN

Accession

Primary (citable) accession number: Q701P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)