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Protein

Phosphoglucan, water dikinase, chloroplastic

Gene

GWD3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the incorporation of phosphate into starch-like phospho-alpha-glucan, mostly at the C-3 position of glucose units. Required for starch degradation, suggesting that the phosphate content of starch regulates its degradability.2 Publications

Catalytic activityi

ATP + (phospho-alpha-glucan) + H2O = AMP + O-phospho-(phospho-alpha-glucan) + phosphate.2 Publications

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei759Tele-phosphohistidine intermediate1 Publication1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • carbohydrate kinase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phosphoglucan, water dikinase activity Source: TAIR
  • starch binding Source: InterPro

GO - Biological processi

  • protein autophosphorylation Source: TAIR
  • starch catabolic process Source: TAIR
  • starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G26570-MONOMER.
MetaCyc:AT5G26570-MONOMER.

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglucan, water dikinase, chloroplastic (EC:2.7.9.5)
Gene namesi
Name:GWD3
Synonyms:OK1, PWD
Ordered Locus Names:At5g26570
ORF Names:F9D12.1, F9D12.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G26570.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 54ChloroplastCombined sourcesAdd BLAST54
ChainiPRO_000024025055 – 1196Phosphoglucan, water dikinase, chloroplasticAdd BLAST1142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6ZY51.
PRIDEiQ6ZY51.

PTM databases

iPTMnetiQ6ZY51.

Expressioni

Tissue specificityi

In all starch containing tissues (e.g. roots, leaves, stems, inflorescence and siliques).1 Publication

Inductioni

Circadian regulation. Induced during light phase and repressed during dark phase.1 Publication

Gene expression databases

ExpressionAtlasiQ6ZY51. baseline and differential.
GenevisibleiQ6ZY51. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi17981. 1 interactor.
STRINGi3702.AT5G26570.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ZY51.
SMRiQ6ZY51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 166CBM20PROSITE-ProRule annotationAdd BLAST101

Domaini

The N-terminal domain contains the alpha-glucan binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the ATP binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIIJ. Eukaryota.
COG0574. LUCA.
HOGENOMiHOG000265182.
InParanoidiQ6ZY51.
KOiK15535.
OMAiGKDKIWE.
OrthoDBiEOG093600ON.
PhylomeDBiQ6ZY51.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR013783. Ig-like_fold.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q6ZY51-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESIGSHCCS SPFTFITRNS SSSLPRLVNI THRVNLSHQS HRLRNSNSRL
60 70 80 90 100
TCTATSSSTI EEQRKKKDGS GTKVRLNVRL DHQVNFGDHV AMFGSAKEIG
110 120 130 140 150
SWKKKSPLNW SENGWVCELE LDGGQVLEYK FVIVKNDGSL SWESGDNRVL
160 170 180 190 200
KVPNSGNFSV VCHWDATRET LDLPQEVGND DDVGDGGHER DNHDVGDDRV
210 220 230 240 250
VGSENGAQLQ KSTLGGQWQG KDASFMRSND HGNREVGRNW DTSGLEGTAL
260 270 280 290 300
KMVEGDRNSK NWWRKLEMVR EVIVGSVERE ERLKALIYSA IYLKWINTGQ
310 320 330 340 350
IPCFEDGGHH RPNRHAEISR LIFRELEHIC SKKDATPEEV LVARKIHPCL
360 370 380 390 400
PSFKAEFTAA VPLTRIRDIA HRNDIPHDLK QEIKHTIQNK LHRNAGPEDL
410 420 430 440 450
IATEAMLQRI TETPGKYSGD FVEQFKIFHN ELKDFFNAGS LTEQLDSMKI
460 470 480 490 500
SMDDRGLSAL NLFFECKKRL DTSGESSNVL ELIKTMHSLA SLRETIIKEL
510 520 530 540 550
NSGLRNDAPD TAIAMRQKWR LCEIGLEDYF FVLLSRFLNA LETMGGADQL
560 570 580 590 600
AKDVGSRNVA SWNDPLDALV LGVHQVGLSG WKQEECLAIG NELLAWRERD
610 620 630 640 650
LLEKEGEEDG KTIWAMRLKA TLDRARRLTA EYSDLLLQIF PPNVEILGKA
660 670 680 690 700
LGIPENSVKT YTEAEIRAGI IFQISKLCTV LLKAVRNSLG SEGWDVVVPG
710 720 730 740 750
STSGTLVQVE SIVPGSLPAT SGGPIILLVN KADGDEEVSA ANGNIAGVML
760 770 780 790 800
LQELPHLSHL GVRARQEKIV FVTCDDDDKV ADIRRLVGKF VRLEASPSHV
810 820 830 840 850
NLILSTEGRS RTSKSSATKK TDKNSLSKKK TDKKSLSIDD EESKPGSSSS
860 870 880 890 900
NSLLYSSKDI PSGGIIALAD ADVPTSGSKS AACGLLASLA EASSKVHSEH
910 920 930 940 950
GVPASFKVPT GVVIPFGSME LALKQNNSEE KFASLLEKLE TARPEGGELD
960 970 980 990 1000
DICDQIHEVM KTLQVPKETI NSISKAFLKD ARLIVRSSAN VEDLAGMSAA
1010 1020 1030 1040 1050
GLYESIPNVS PSDPLVFSDS VCQVWASLYT RRAVLSRRAA GVSQREASMA
1060 1070 1080 1090 1100
VLVQEMLSPD LSFVLHTVSP ADPDSNLVEA EIAPGLGETL ASGTRGTPWR
1110 1120 1130 1140 1150
LASGKLDGIV QTLAFANFSE ELLVSGTGPA DGKYVRLTVD YSKKRLTVDS
1160 1170 1180 1190
VFRQQLGQRL GSVGFFLERN FGCAQDVEGC LVGEDVYIVQ SRPQPL
Length:1,196
Mass (Da):131,323
Last modified:July 5, 2004 - v1
Checksum:i98A320AC0484532B
GO

Sequence cautioni

The sequence AAC26245 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC26246 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52C → R in AAU93516 (PubMed:15686522).Curated1
Sequence conflicti129Y → C in AAU93516 (PubMed:15686522).Curated1
Sequence conflicti582K → R in AAO64153 (PubMed:14593172).Curated1
Sequence conflicti1109I → V in AAO64153 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY747068 mRNA. Translation: AAU93516.1.
AJ635427 mRNA. Translation: CAG25776.1.
AF077407 Genomic DNA. Translation: AAC26245.1. Sequence problems.
AF077407 Genomic DNA. Translation: AAC26246.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93555.1.
BT005745 mRNA. Translation: AAO64153.1.
PIRiT01856.
T01857.
RefSeqiNP_198009.3. NM_122538.5. [Q6ZY51-1]
UniGeneiAt.20942.

Genome annotation databases

EnsemblPlantsiAT5G26570.1; AT5G26570.1; AT5G26570. [Q6ZY51-1]
GeneIDi832706.
GrameneiAT5G26570.1; AT5G26570.1; AT5G26570.
KEGGiath:AT5G26570.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY747068 mRNA. Translation: AAU93516.1.
AJ635427 mRNA. Translation: CAG25776.1.
AF077407 Genomic DNA. Translation: AAC26245.1. Sequence problems.
AF077407 Genomic DNA. Translation: AAC26246.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93555.1.
BT005745 mRNA. Translation: AAO64153.1.
PIRiT01856.
T01857.
RefSeqiNP_198009.3. NM_122538.5. [Q6ZY51-1]
UniGeneiAt.20942.

3D structure databases

ProteinModelPortaliQ6ZY51.
SMRiQ6ZY51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17981. 1 interactor.
STRINGi3702.AT5G26570.1.

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.

PTM databases

iPTMnetiQ6ZY51.

Proteomic databases

PaxDbiQ6ZY51.
PRIDEiQ6ZY51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G26570.1; AT5G26570.1; AT5G26570. [Q6ZY51-1]
GeneIDi832706.
GrameneiAT5G26570.1; AT5G26570.1; AT5G26570.
KEGGiath:AT5G26570.

Organism-specific databases

TAIRiAT5G26570.

Phylogenomic databases

eggNOGiENOG410IIIJ. Eukaryota.
COG0574. LUCA.
HOGENOMiHOG000265182.
InParanoidiQ6ZY51.
KOiK15535.
OMAiGKDKIWE.
OrthoDBiEOG093600ON.
PhylomeDBiQ6ZY51.

Enzyme and pathway databases

BioCyciARA:AT5G26570-MONOMER.
MetaCyc:AT5G26570-MONOMER.

Miscellaneous databases

PROiQ6ZY51.

Gene expression databases

ExpressionAtlasiQ6ZY51. baseline and differential.
GenevisibleiQ6ZY51. AT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR013783. Ig-like_fold.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPWD_ARATH
AccessioniPrimary (citable) accession number: Q6ZY51
Secondary accession number(s): O81504
, O81505, Q5XMK9, Q84TI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the C-terminal domain, and the third partial reaction is catalyzed at an active site located on the N-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the C-terminal domain to that of the N-terminal domain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.