Reviewed, UniProtKB/Swiss-Prot Q6ZXD2 (SIA8A_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
    EC=2.4.99.8
Alternative name(s):
    Ganglioside GD3 synthase
    Ganglioside GT3 synthase
    Alpha-2,8-sialyltransferase 8A
    Sialytransferase St8Sia-I
      Short name=ST8Sia I

Gene names

Name:	ST8SIA1
Synonyms:	SIAT8A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid By similarity.
Catalytic activity	CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R.
Pathway	Protein modification; protein glycosylation. Lipid metabolism; sphingolipid metabolism.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Biological process	Lipid metabolism Lipid synthesis Sphingolipid metabolism
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase		PRO_0000247930
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 48	19	Signal-anchor for type II membrane protein Potential
	Topological domain	49 – 356	308	Lumenal Potential
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	214	1	N-linked (GlcNAc...) Potential
	Glycosylation	245	1	N-linked (GlcNAc...) Potential
	Disulfide bond	138 ↔ 287		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZXD2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A6B2DE0A9C36076F	FASTA	356	40,452
	10 20 30 40 50 60 MSPCGRARRH TSRGAMAVLA WKFPRTRLPV GASALCVVVL CWLYVFPVYR LPDEKEIVQG 70 80 90 100 110 120 VLQQGTAWRR NRTAAGIFRK QMEDCCDPAH LFAMTKMNAP MGKSLWYDGE FLYSFTIDNS 130 140 150 160 170 180 TYSLFPQATP FQLPLKKCAV VGNGGILKKS GCGRQIDEAD FVMRCNLPPL SSEYTKDVGS 190 200 210 220 230 240 KSHLVTANPS IIRQRFQNLL WSRKTFVDHM KVYNHSYIYM PAFSMKTGTE PSLRVYYTLS 250 260 270 280 290 300 DVGANQTVLF ANPNFLRSIG KFWKSRGIHA KRLSTGLFLV SAALGLCEEV AIYGFWPFSV 310 320 330 340 350 NMHEQPISHH YYDNVLPFSG FHAMPEEFLQ LWYLHKIGAL RMQLDPCEDN SLQPTS

References

[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ699418 mRNA. Translation: CAG27880.1.
IPI	IPI00685831.
RefSeq	NP_001027470.1.
UniGene	Bt.13269
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6ZXD2.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
Ensembl	ENSBTAT00000000780; ENSBTAP00000000780; ENSBTAG00000000593; Bos taurus. [Genome view]
GeneID	282352.
KEGG	bta:282352.
Organism-specific databases
CTD	282352.
Phylogenomic databases
HOVERGEN	Q6ZXD2.
InParanoid	Q6ZXD2.
Enzyme and pathway databases
BRENDA	2.4.99.8. 251.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIA8A_BOVIN

Accession

Primary (citable) accession number: Q6ZXD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	December 15, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)