Reviewed, UniProtKB/Swiss-Prot Q6ZXC9 (SIA8D_BOVIN)

Names and origin

Protein names

Recommended name:
    CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
    EC=2.4.99.-
Alternative name(s):
    Alpha-2,8-sialyltransferase 8D
    ST8Sia IV
    Polysialyltransferase-1

Gene names

Name:	ST8SIA4
Synonyms:	SIAT8D

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase		PRO_0000247931
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 20	13	Signal-anchor for type II membrane protein Potential
	Topological domain	21 – 359	339	Lumenal Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Disulfide bond	142 ↔ 292		By similarity
	Disulfide bond	156 ↔ 356		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZXC9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C7C0B4FFCDA967C4	FASTA	359	41,162
	10 20 30 40 50 60 MRSIRKRWTI CTISLLLIFY KTKEMARTEE HQETQLIGDG ELSLSRSLVN SSDKIIRKAG 70 80 90 100 110 120 SSIFQHSVEG GKINSSLVLE IRKNILRFLD AERDVSVVKS SFKPGDVIHY VLDRRRTLNI 130 140 150 160 170 180 SQDLHSLLPE VSPMKNRRFK TCAVVGNSGI LLDSECGKEI DSHNFVIRCN LAPVVEFAAD 190 200 210 220 230 240 VGTKSDFITM NPSVVQRAFG GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV 250 260 270 280 290 300 NALILKNKLK VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF 310 320 330 340 350 WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK LTTGKCIKQ

References

[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ699421 mRNA. Translation: CAG27883.1.
IPI	IPI00701492.
RefSeq	NP_001001163.1.
UniGene	Bt.17789
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6ZXC9.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
Ensembl	ENSBTAT00000028590; ENSBTAP00000028590; ENSBTAG00000021445; Bos taurus. [Genome view]
GeneID	407768.
KEGG	bta:407768.
Organism-specific databases
CTD	407768.
Phylogenomic databases
HOVERGEN	Q6ZXC9.
InParanoid	Q6ZXC9.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIA8D_BOVIN

Accession

Primary (citable) accession number: Q6ZXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	December 15, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)