Reviewed, UniProtKB/Swiss-Prot Q6ZWZ2 (UB2R2_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 R2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase R2
    Ubiquitin carrier protein R2
    E2-CDC34B

Gene names

Name:	Ube2r2
Synonyms:	Cdc34b, Ubc3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	When phosphorylated, interacts with beta-TrCP (BTRC) By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ubiquitin-conjugating enzyme E2 R2		PRO_0000280514
Regions
	Compositional bias	200 – 238	39	Asp/Glu-rich (acidic)
Sites
	Active site	93	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	233	1	Phosphoserine; by CK2 By similarity
Experimental info
	Sequence conflict	16	1	E → D in BAC35899. Ref.2
	Sequence conflict	29	1	R → L in BAC35899. Ref.2
	Sequence conflict	33	1	V → L in BAC35899. Ref.2
	Sequence conflict	35	1	E → Y in BAC35899. Ref.2
	Sequence conflict	37	1	D → Y in BAC35899. Ref.2
	Sequence conflict	149	1	R → T in CAC80335. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6ZWZ2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E896CF0116A56308	FASTA	238	27,166
	10 20 30 40 50 60 MAQQQMTSSQ KALMLELKSL QEEPVEGFRI TLVDESDLYN WEVAIFGPPN TLYEGGYFKA 70 80 90 100 110 120 HIKFPIDYPY SPPTFRFLTK MWHPNIYENG DVCISILHPP VDDPQSGELP SERWNPTQNV 130 140 150 160 170 180 RTILLSVISL LNEPNTFSPA NVDASVMFRK WRDSKGKDKE YAEIIRKQVS ATKAEAEKDG 190 200 210 220 230 VKVPTTLAEY CIKTKVPSND NSSDLLYDDL YDDDIDDEDE EEEDADCYDD DDSGNEES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation." Semplici F., Meggio F., Pinna L.A., Oliviero S. Oncogene 21:3978-3987(2002) [PubMed: 12037680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver, Lung and Pancreas.
[3]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ240086 mRNA. Translation: CAC80335.1. AK003550 mRNA. Translation: BAB22850.1. AK007517 mRNA. Translation: BAB25085.1. AK075703 mRNA. Translation: BAC35899.1. AK075714 mRNA. Translation: BAC35904.1. AL807823, AL954379 Genomic DNA. Translation: CAM20979.1. AL807823, AL954379 Genomic DNA. Translation: CAM27658.1. BC011112 mRNA. Translation: AAH11112.1.
RefSeq	NP_080551.1.
UniGene	Mm.389540
3D structure databases
HSSP	HSSP built from PDB template 1A3S based on UniProtKB P50550.
SMR	Q6ZWZ2. Positions 9-181.
ModBase	Search...
PTM databases
PhosphoSite	Q6ZWZ2.
Genome annotation databases
Ensembl	ENSMUSG00000036241. Mus musculus. [Contig view]
GeneID	67615.
KEGG	mmu:67615.
Organism-specific databases
MGI	MGI:1914865. Ube2r2.
Phylogenomic databases
HOVERGEN	Q6ZWZ2.
Gene expression databases
ArrayExpress	Q6ZWZ2.
CleanEx	MM_UBE2R2.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	325049.
SOURCE	Search...

Entry information

Entry name

UB2R2_MOUSE

Accession

Primary (citable) accession number: Q6ZWZ2
Secondary accession number(s): Q8BW18, Q8VDE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)