Q6ZWX6 (IF2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 2 subunit 1

Alternative name(s):
Eukaryotic translation initiation factor 2 subunit alpha
Short name=eIF-2-alpha
Short name=eIF-2A
Short name=eIF-2alpha

Gene names

Name:	Eif2s1
Synonyms:	Eif2a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B By similarity.
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain. Interacts with ABCF1. Associates with ribosomes. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Ref.3
Subcellular location	Cytoplasmic granule. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules. Ref.4
Post-translational modification	Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation By similarity.
Sequence similarities	Belongs to the eIF-2-alpha family. Contains 1 S1 motif domain.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Ligand	RNA-binding
Molecular function	Initiation factor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein autophosphorylation Inferred from direct assay PubMed 19139267. Source: MGI regulation of translational initiation in response to stress Inferred from direct assay PubMed 15937339. Source: MGI
Cellular_component	cytoplasmic stress granule Inferred from direct assay Ref.4. Source: UniProtKB eukaryotic translation initiation factor 2 complex Inferred from electronic annotation. Source: InterPro eukaryotic translation initiation factor 2B complex Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 12388085. Source: MGI
Molecular_function	ribosome binding Inferred from sequence or structural similarity. Source: UniProtKB translation initiation factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Eukaryotic translation initiation factor 2 subunit 1		PRO_0000137383
Regions
	Domain	17 – 88	72	S1 motif
Amino acid modifications
	Modified residue	49	1	Phosphoserine; by HRI By similarity
	Modified residue	52	1	Phosphoserine; by EIF2AK3, GCN2, HRI and PKR By similarity
	Modified residue	141	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZWX6 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 018480B89175D118	FASTA	315	36,108
	10 20 30 40 50 60 MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN 70 80 90 100 110 120 KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE 130 140 150 160 170 180 YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI 190 200 210 220 230 240 NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ETMPIKINLI APPRYVMTTT 250 260 270 280 290 300 TLERTEGLSV LNQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQL ERLERENAEV 310 DGDDDAEEME AKAED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Eye, Kidney and Mammary tumor.
[3]	"Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver." Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A. J. Biol. Chem. 281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH CELF1; EIF2S2; CALR; CALR3; HSPA5 AND HSP90B1.
[4]	"Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3." Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M. Reproduction 139:381-393(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK010592 mRNA. Translation: BAB27049.1. AK143499 mRNA. Translation: BAE25400.1. AK167763 mRNA. Translation: BAE39796.1. BC005463 mRNA. Translation: AAH05463.1. BC016448 mRNA. Translation: AAH16448.1. BC016497 mRNA. Translation: AAH16497.1.
IPI	IPI00474446.
RefSeq	NP_080390.1. NM_026114.3.
UniGene	Mm.196220.
3D structure databases
ProteinModelPortal	Q6ZWX6.
SMR	Q6ZWX6. Positions 4-303.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZWX6. 5 interactions.
STRING	10090.ENSMUSP00000071214.
PTM databases
PhosphoSite	Q6ZWX6.
Proteomic databases
PaxDb	Q6ZWX6.
PRIDE	Q6ZWX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000071230; ENSMUSP00000071214; ENSMUSG00000021116.
GeneID	13665.
KEGG	mmu:13665.
UCSC	uc007nzk.1. mouse.
Organism-specific databases
CTD	1965.
MGI	MGI:95299. Eif2s1.
Phylogenomic databases
eggNOG	COG1093.
GeneTree	ENSGT00390000007015.
HOGENOM	HOG000199476.
HOVERGEN	HBG001910.
InParanoid	Q6ZWX6.
KO	K03237.
OMA	EKCTERF.
OrthoDB	EOG4BZN35.
Gene expression databases
ArrayExpress	Q6ZWX6.
Bgee	Q6ZWX6.
CleanEx	MM_EIF2A.
Genevestigator	Q6ZWX6.
GermOnline	ENSMUSG00000021116. Mus musculus.
Family and domain databases
Gene3D	1.10.150.190. 1 hit. 2.40.50.140. 1 hit. 3.30.70.1130. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR022967. RNA-binding_domain_S1. IPR024055. TIF2_asu_C. IPR024054. TIF2_asu_middle. IPR011488. TIF_2_asu. [Graphical view]
Pfam	PF07541. EIF_2_alpha. 1 hit. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF110993. SSF110993. 1 hit. SSF116742. SSF116742. 1 hit.
PROSITE	PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1770036.
NextBio	284416.
SOURCE	Search...

Entry information

Entry name

IF2A_MOUSE

Accession

Primary (citable) accession number: Q6ZWX6
Secondary accession number(s): Q3TIQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program