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Protein

Eukaryotic translation initiation factor 2 subunit 1

Gene

Eif2s1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • cellular response to amino acid starvation Source: UniProtKB
  • cellular response to heat Source: Ensembl
  • cellular response to oxidative stress Source: ARUK-UCL
  • cellular response to UV Source: UniProtKB
  • negative regulation of guanyl-nucleotide exchange factor activity Source: Ensembl
  • negative regulation of translational initiation in response to stress Source: UniProtKB
  • positive regulation of neuron death Source: MGI
  • positive regulation of type B pancreatic cell apoptotic process Source: Ensembl
  • protein autophosphorylation Source: MGI
  • regulation of translational initiation in response to stress Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • response to manganese-induced endoplasmic reticulum stress Source: Ensembl
  • stress granule assembly Source: ARUK-UCL
  • translation Source: MGI

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-381042 PERK regulates gene expression
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-72649 Translation initiation complex formation
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 1
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Short name:
eIF-2A
Short name:
eIF-2alpha
Gene namesi
Name:Eif2s1
Synonyms:Eif2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:95299 Eif2s1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1770036

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373831 – 315Eukaryotic translation initiation factor 2 subunit 1Add BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49Phosphoserine; by HRIBy similarity1
Modified residuei52Phosphoserine4 Publications1
Modified residuei141N6-acetyllysineBy similarity1
Modified residuei158PhosphoserineCombined sources1
Modified residuei279PhosphothreonineBy similarity1
Modified residuei281PhosphothreonineBy similarity1

Post-translational modificationi

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (By similarity). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (PubMed:10504407, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16054071).By similarity5 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZWX6
MaxQBiQ6ZWX6
PaxDbiQ6ZWX6
PeptideAtlasiQ6ZWX6
PRIDEiQ6ZWX6

PTM databases

iPTMnetiQ6ZWX6
PhosphoSitePlusiQ6ZWX6
SwissPalmiQ6ZWX6

Expressioni

Gene expression databases

BgeeiENSMUSG00000021116
CleanExiMM_EIF2A
GenevisibleiQ6ZWX6 MM

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Interacts with ABCF1. Associates with ribosomes. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Eif2ak1Q9Z2R96EBI-1202234,EBI-642878

Protein-protein interaction databases

BioGridi199412, 5 interactors
IntActiQ6ZWX6, 11 interactors
MINTiQ6ZWX6
STRINGi10090.ENSMUSP00000071214

Structurei

3D structure databases

ProteinModelPortaliQ6ZWX6
SMRiQ6ZWX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 88S1 motifPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated

Phylogenomic databases

eggNOGiKOG2916 Eukaryota
COG1093 LUCA
GeneTreeiENSGT00390000007015
HOGENOMiHOG000199476
HOVERGENiHBG001910
InParanoidiQ6ZWX6
KOiK03237
OMAiKDVNEHQ
OrthoDBiEOG091G07NN
PhylomeDBiQ6ZWX6
TreeFamiTF101502

Family and domain databases

Gene3Di1.10.150.190, 1 hit
3.30.70.1130, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022967 S1_dom
IPR003029 S1_domain
IPR024055 TIF2_asu_C
IPR024054 TIF2_asu_middle_sf
IPR011488 TIF_2_asu
PANTHERiPTHR10602 PTHR10602, 1 hit
PfamiView protein in Pfam
PF07541 EIF_2_alpha, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF110993 SSF110993, 1 hit
SSF116742 SSF116742, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q6ZWX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE
60 70 80 90 100
LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED
110 120 130 140 150
KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY
160 170 180 190 200
DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY
210 220 230 240 250
GYEGIDAVKE ALRAGLNCST ETMPIKINLI APPRYVMTTT TLERTEGLSV
260 270 280 290 300
LNQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQL ERLERENAEV
310
DGDDDAEEME AKAED
Length:315
Mass (Da):36,108
Last modified:January 23, 2007 - v3
Checksum:i018480B89175D118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010592 mRNA Translation: BAB27049.1
AK143499 mRNA Translation: BAE25400.1
AK167763 mRNA Translation: BAE39796.1
BC005463 mRNA Translation: AAH05463.1
BC016448 mRNA Translation: AAH16448.1
BC016497 mRNA Translation: AAH16497.1
CCDSiCCDS26003.1
RefSeqiNP_080390.1, NM_026114.3
UniGeneiMm.196220

Genome annotation databases

EnsembliENSMUST00000071230; ENSMUSP00000071214; ENSMUSG00000021116
GeneIDi13665
KEGGimmu:13665
UCSCiuc007nzk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIF2A_MOUSE
AccessioniPrimary (citable) accession number: Q6ZWX6
Secondary accession number(s): Q3TIQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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