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Protein

Eukaryotic translation initiation factor 2 subunit 1

Gene

Eif2s1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-381042. PERK regulates gene expression.
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 1
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Short name:
eIF-2A
Short name:
eIF-2alpha
Gene namesi
Name:Eif2s1
Synonyms:Eif2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:95299. Eif2s1.

Subcellular locationi

  • Cytoplasmic granule 1 Publication

  • Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules.

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1770036.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Eukaryotic translation initiation factor 2 subunit 1PRO_0000137383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491Phosphoserine; by HRIBy similarity
Modified residuei52 – 521Phosphoserine4 Publications
Modified residuei141 – 1411N6-acetyllysineBy similarity
Modified residuei158 – 1581PhosphoserineCombined sources
Modified residuei279 – 2791PhosphothreonineBy similarity
Modified residuei281 – 2811PhosphothreonineBy similarity

Post-translational modificationi

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (By similarity). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (PubMed:10504407, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16054071).By similarity5 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZWX6.
MaxQBiQ6ZWX6.
PaxDbiQ6ZWX6.
PeptideAtlasiQ6ZWX6.
PRIDEiQ6ZWX6.

PTM databases

iPTMnetiQ6ZWX6.
PhosphoSiteiQ6ZWX6.
SwissPalmiQ6ZWX6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021116.
CleanExiMM_EIF2A.
GenevisibleiQ6ZWX6. MM.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Interacts with ABCF1. Associates with ribosomes. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Eif2ak1Q9Z2R96EBI-1202234,EBI-642878

Protein-protein interaction databases

BioGridi199412. 5 interactions.
IntActiQ6ZWX6. 11 interactions.
STRINGi10090.ENSMUSP00000071214.

Structurei

3D structure databases

ProteinModelPortaliQ6ZWX6.
SMRiQ6ZWX6. Positions 4-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 8872S1 motifPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2916. Eukaryota.
COG1093. LUCA.
GeneTreeiENSGT00390000007015.
HOGENOMiHOG000199476.
HOVERGENiHBG001910.
InParanoidiQ6ZWX6.
KOiK03237.
OMAiGVFNIQM.
OrthoDBiEOG091G07NN.
PhylomeDBiQ6ZWX6.
TreeFamiTF101502.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ZWX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE
60 70 80 90 100
LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED
110 120 130 140 150
KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY
160 170 180 190 200
DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY
210 220 230 240 250
GYEGIDAVKE ALRAGLNCST ETMPIKINLI APPRYVMTTT TLERTEGLSV
260 270 280 290 300
LNQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQL ERLERENAEV
310
DGDDDAEEME AKAED
Length:315
Mass (Da):36,108
Last modified:January 23, 2007 - v3
Checksum:i018480B89175D118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010592 mRNA. Translation: BAB27049.1.
AK143499 mRNA. Translation: BAE25400.1.
AK167763 mRNA. Translation: BAE39796.1.
BC005463 mRNA. Translation: AAH05463.1.
BC016448 mRNA. Translation: AAH16448.1.
BC016497 mRNA. Translation: AAH16497.1.
CCDSiCCDS26003.1.
RefSeqiNP_080390.1. NM_026114.3.
UniGeneiMm.196220.

Genome annotation databases

EnsembliENSMUST00000071230; ENSMUSP00000071214; ENSMUSG00000021116.
GeneIDi13665.
KEGGimmu:13665.
UCSCiuc007nzk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010592 mRNA. Translation: BAB27049.1.
AK143499 mRNA. Translation: BAE25400.1.
AK167763 mRNA. Translation: BAE39796.1.
BC005463 mRNA. Translation: AAH05463.1.
BC016448 mRNA. Translation: AAH16448.1.
BC016497 mRNA. Translation: AAH16497.1.
CCDSiCCDS26003.1.
RefSeqiNP_080390.1. NM_026114.3.
UniGeneiMm.196220.

3D structure databases

ProteinModelPortaliQ6ZWX6.
SMRiQ6ZWX6. Positions 4-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199412. 5 interactions.
IntActiQ6ZWX6. 11 interactions.
STRINGi10090.ENSMUSP00000071214.

Chemistry

ChEMBLiCHEMBL1770036.

PTM databases

iPTMnetiQ6ZWX6.
PhosphoSiteiQ6ZWX6.
SwissPalmiQ6ZWX6.

Proteomic databases

EPDiQ6ZWX6.
MaxQBiQ6ZWX6.
PaxDbiQ6ZWX6.
PeptideAtlasiQ6ZWX6.
PRIDEiQ6ZWX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071230; ENSMUSP00000071214; ENSMUSG00000021116.
GeneIDi13665.
KEGGimmu:13665.
UCSCiuc007nzk.1. mouse.

Organism-specific databases

CTDi1965.
MGIiMGI:95299. Eif2s1.

Phylogenomic databases

eggNOGiKOG2916. Eukaryota.
COG1093. LUCA.
GeneTreeiENSGT00390000007015.
HOGENOMiHOG000199476.
HOVERGENiHBG001910.
InParanoidiQ6ZWX6.
KOiK03237.
OMAiGVFNIQM.
OrthoDBiEOG091G07NN.
PhylomeDBiQ6ZWX6.
TreeFamiTF101502.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-381042. PERK regulates gene expression.
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ6ZWX6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021116.
CleanExiMM_EIF2A.
GenevisibleiQ6ZWX6. MM.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2A_MOUSE
AccessioniPrimary (citable) accession number: Q6ZWX6
Secondary accession number(s): Q3TIQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.