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Protein

Speckle-type POZ protein

Gene

Spop

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL (By similarity). In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation.By similarity4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • glucose homeostasis Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of type B pancreatic cell apoptotic process Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
  • regulation of proteolysis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5632684. Hedgehog 'on' state.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein
Alternative name(s):
HIB homolog 1
PDX-1 C-terminal-interacting factor 1
Gene namesi
Name:Spop
Synonyms:Pcif1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1343085. Spop.

Subcellular locationi

  • Nucleus 1 Publication
  • Nucleus speckle 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001916221 – 374Speckle-type POZ proteinAdd BLAST374

Proteomic databases

PaxDbiQ6ZWS8.
PRIDEiQ6ZWS8.

PTM databases

iPTMnetiQ6ZWS8.
PhosphoSitePlusiQ6ZWS8.

Expressioni

Tissue specificityi

Widely expressed, mainly in pancreas and in particular in adult pancreatic insulin-producing beta cells and in a subset of exocrine acinar and duct cells.

Gene expression databases

BgeeiENSMUSG00000057522.
CleanExiMM_SPOP.
ExpressionAtlasiQ6ZWS8. baseline and differential.
GenevisibleiQ6ZWS8. MM.

Interactioni

Subunit structurei

Homodimer and homooligomer. Heterodimer with SPOPL. Each dimer interacts with two CUL3 molecules. Part of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain CUL3 and homodimeric SPOP, or the heterodimer formed by SPOP and SPOPL, plus a target protein, such as H2AFY, PDX1/IPF1, BMI1, BRMS1 and DAXX (By similarity). Interacts with H2AFY, PDX1/IPF1, GLI2 and GLI3.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pdx1P529465EBI-7128920,EBI-7128945

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203465. 4 interactors.
DIPiDIP-43930N.
IntActiQ6ZWS8. 1 interactor.
MINTiMINT-3982111.
STRINGi10090.ENSMUSP00000103350.

Structurei

3D structure databases

ProteinModelPortaliQ6ZWS8.
SMRiQ6ZWS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 161MATHPROSITE-ProRule annotationAdd BLAST131
Domaini200 – 267BTBPROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 191Required for nuclear localizationBy similarityAdd BLAST121
Regioni123 – 133Important for binding substrate proteinsBy similarityAdd BLAST11
Regioni186 – 217Important for homodimerizationBy similarityAdd BLAST32
Regioni297 – 355Important for homodimerizationBy similarityAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 10Poly-Pro6

Domaini

The BTB (POZ) domain mediates dimerization and interaction with CUL3.By similarity
The MATH domain mediates interaction with protein-ubiquitin ligase substrates, such as H2AFY and BMI1.

Sequence similaritiesi

Belongs to the Tdpoz family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 1 MATH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ6ZWS8.
KOiK10523.
OMAiWHSSHAS.
OrthoDBiEOG091G07ZM.
PhylomeDBiQ6ZWS8.
TreeFamiTF313419.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ZWS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT
210 220 230 240 250
DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV
260 270 280 290 300
FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE
310 320 330 340 350
NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA
360 370
EAYRSLASAQ CPFLGPPRKR LKQS
Length:374
Mass (Da):42,132
Last modified:July 5, 2004 - v1
Checksum:iEE5F4C5CF6FD09DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54S → G in BAE38870 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY538613 mRNA. Translation: AAT08952.1.
AK028201 mRNA. Translation: BAC25809.1.
AK030746 mRNA. Translation: BAC27114.1.
AK159482 mRNA. Translation: BAE35119.1.
AK166581 mRNA. Translation: BAE38870.1.
AL662875 Genomic DNA. Translation: CAI25973.1.
BC043131 mRNA. Translation: AAH43131.1.
BC045205 mRNA. Translation: AAH45205.1.
AB071989 mRNA. Translation: BAB68542.1.
CCDSiCCDS36286.1.
RefSeqiNP_079563.2. NM_025287.2.
XP_006532755.1. XM_006532692.2.
UniGeneiMm.285454.

Genome annotation databases

EnsembliENSMUST00000107722; ENSMUSP00000103350; ENSMUSG00000057522.
ENSMUST00000107724; ENSMUSP00000103352; ENSMUSG00000057522.
GeneIDi20747.
KEGGimmu:20747.
UCSCiuc007laj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY538613 mRNA. Translation: AAT08952.1.
AK028201 mRNA. Translation: BAC25809.1.
AK030746 mRNA. Translation: BAC27114.1.
AK159482 mRNA. Translation: BAE35119.1.
AK166581 mRNA. Translation: BAE38870.1.
AL662875 Genomic DNA. Translation: CAI25973.1.
BC043131 mRNA. Translation: AAH43131.1.
BC045205 mRNA. Translation: AAH45205.1.
AB071989 mRNA. Translation: BAB68542.1.
CCDSiCCDS36286.1.
RefSeqiNP_079563.2. NM_025287.2.
XP_006532755.1. XM_006532692.2.
UniGeneiMm.285454.

3D structure databases

ProteinModelPortaliQ6ZWS8.
SMRiQ6ZWS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203465. 4 interactors.
DIPiDIP-43930N.
IntActiQ6ZWS8. 1 interactor.
MINTiMINT-3982111.
STRINGi10090.ENSMUSP00000103350.

PTM databases

iPTMnetiQ6ZWS8.
PhosphoSitePlusiQ6ZWS8.

Proteomic databases

PaxDbiQ6ZWS8.
PRIDEiQ6ZWS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107722; ENSMUSP00000103350; ENSMUSG00000057522.
ENSMUST00000107724; ENSMUSP00000103352; ENSMUSG00000057522.
GeneIDi20747.
KEGGimmu:20747.
UCSCiuc007laj.1. mouse.

Organism-specific databases

CTDi8405.
MGIiMGI:1343085. Spop.

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ6ZWS8.
KOiK10523.
OMAiWHSSHAS.
OrthoDBiEOG091G07ZM.
PhylomeDBiQ6ZWS8.
TreeFamiTF313419.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-5632684. Hedgehog 'on' state.

Miscellaneous databases

ChiTaRSiSpop. mouse.
PROiQ6ZWS8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057522.
CleanExiMM_SPOP.
ExpressionAtlasiQ6ZWS8. baseline and differential.
GenevisibleiQ6ZWS8. MM.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPOP_MOUSE
AccessioniPrimary (citable) accession number: Q6ZWS8
Secondary accession number(s): Q3TLC2, Q5ST07, Q76LV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.