Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Speckle-type POZ protein

Gene

Spop

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL (By similarity). In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation.By similarity4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • glucose homeostasis Source: MGI
  • negative regulation of DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of type B pancreatic cell apoptotic process Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of proteolysis Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5632684 Hedgehog 'on' state
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein
Alternative name(s):
HIB homolog 1
PDX-1 C-terminal-interacting factor 1
Gene namesi
Name:Spop
Synonyms:Pcif1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1343085 Spop

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001916221 – 374Speckle-type POZ proteinAdd BLAST374

Proteomic databases

MaxQBiQ6ZWS8
PaxDbiQ6ZWS8
PRIDEiQ6ZWS8

PTM databases

iPTMnetiQ6ZWS8
PhosphoSitePlusiQ6ZWS8

Expressioni

Tissue specificityi

Widely expressed, mainly in pancreas and in particular in adult pancreatic insulin-producing beta cells and in a subset of exocrine acinar and duct cells.

Gene expression databases

BgeeiENSMUSG00000057522
CleanExiMM_SPOP
ExpressionAtlasiQ6ZWS8 baseline and differential
GenevisibleiQ6ZWS8 MM

Interactioni

Subunit structurei

Homodimer and homooligomer. Heterodimer with SPOPL. Each dimer interacts with two CUL3 molecules. Part of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain CUL3 and homodimeric SPOP, or the heterodimer formed by SPOP and SPOPL, plus a target protein, such as H2AFY, PDX1/IPF1, BMI1, BRMS1 and DAXX (By similarity). Interacts with H2AFY, PDX1/IPF1, GLI2 and GLI3.By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203465, 4 interactors
DIPiDIP-43930N
IntActiQ6ZWS8, 3 interactors
MINTiQ6ZWS8
STRINGi10090.ENSMUSP00000103350

Structurei

3D structure databases

ProteinModelPortaliQ6ZWS8
SMRiQ6ZWS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 161MATHPROSITE-ProRule annotationAdd BLAST131
Domaini200 – 267BTBPROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 191Required for nuclear localizationBy similarityAdd BLAST121
Regioni123 – 133Important for binding substrate proteinsBy similarityAdd BLAST11
Regioni186 – 217Important for homodimerizationBy similarityAdd BLAST32
Regioni297 – 355Important for homodimerizationBy similarityAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 10Poly-Pro6

Domaini

The BTB (POZ) domain mediates dimerization and interaction with CUL3.By similarity
The MATH domain mediates interaction with protein-ubiquitin ligase substrates, such as H2AFY and BMI1.

Sequence similaritiesi

Belongs to the Tdpoz family.Curated

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000231621
HOVERGENiHBG001393
InParanoidiQ6ZWS8
KOiK10523
OMAiFNYMWTI
OrthoDBiEOG091G07ZM
PhylomeDBiQ6ZWS8
TreeFamiTF313419

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
PF00917 MATH, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit

Sequencei

Sequence statusi: Complete.

Q6ZWS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT
210 220 230 240 250
DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV
260 270 280 290 300
FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE
310 320 330 340 350
NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA
360 370
EAYRSLASAQ CPFLGPPRKR LKQS
Length:374
Mass (Da):42,132
Last modified:July 5, 2004 - v1
Checksum:iEE5F4C5CF6FD09DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54S → G in BAE38870 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY538613 mRNA Translation: AAT08952.1
AK028201 mRNA Translation: BAC25809.1
AK030746 mRNA Translation: BAC27114.1
AK159482 mRNA Translation: BAE35119.1
AK166581 mRNA Translation: BAE38870.1
AL662875 Genomic DNA No translation available.
BC043131 mRNA Translation: AAH43131.1
BC045205 mRNA Translation: AAH45205.1
AB071989 mRNA Translation: BAB68542.1
CCDSiCCDS36286.1
RefSeqiNP_079563.2, NM_025287.2
XP_006532755.1, XM_006532692.2
UniGeneiMm.285454

Genome annotation databases

EnsembliENSMUST00000107722; ENSMUSP00000103350; ENSMUSG00000057522
ENSMUST00000107724; ENSMUSP00000103352; ENSMUSG00000057522
GeneIDi20747
KEGGimmu:20747
UCSCiuc007laj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSPOP_MOUSE
AccessioniPrimary (citable) accession number: Q6ZWS8
Secondary accession number(s): Q3TLC2, Q5ST07, Q76LV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health