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Protein

Syntaxin-binding protein 4

Gene

STXBP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose (By similarity).By similarity

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • glucose transport Source: Ensembl
  • insulin receptor signaling pathway Source: Ensembl
  • positive regulation of cell cycle G1/S phase transition Source: UniProtKB
  • positive regulation of keratinocyte proliferation Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • protein targeting Source: Ensembl
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ6ZWJ1.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 4
Alternative name(s):
Syntaxin 4-interacting protein
Short name:
STX4-interacting protein
Short name:
Synip
Gene namesi
Name:STXBP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:19694. STXBP4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • intermediate filament cytoskeleton Source: HPA
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134962267.

Polymorphism and mutation databases

BioMutaiSTXBP4.
DMDMi296452915.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Syntaxin-binding protein 4PRO_0000076330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei99 – 991PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZWJ1.
MaxQBiQ6ZWJ1.
PaxDbiQ6ZWJ1.
PRIDEiQ6ZWJ1.

PTM databases

iPTMnetiQ6ZWJ1.
PhosphoSiteiQ6ZWJ1.

Expressioni

Gene expression databases

BgeeiQ6ZWJ1.
CleanExiHS_STXBP4.
ExpressionAtlasiQ6ZWJ1. baseline and differential.
GenevisibleiQ6ZWJ1. HS.

Organism-specific databases

HPAiCAB007760.
HPA022138.
HPA022888.
HPA024227.

Interactioni

Subunit structurei

Interacts with STX4A.By similarity

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi128947. 26 interactions.
IntActiQ6ZWJ1. 9 interactions.
MINTiMINT-2879195.
STRINGi9606.ENSP00000365530.

Structurei

Secondary structure

1
553
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi502 – 5065Combined sources
Beta strandi508 – 5103Combined sources
Beta strandi512 – 5198Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YSGNMR-A498-530[»]
ProteinModelPortaliQ6ZWJ1.
SMRiQ6ZWJ1. Positions 14-107, 498-530.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6ZWJ1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 10587PDZPROSITE-ProRule annotationAdd
BLAST
Domaini496 – 52934WWPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili291 – 417127Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 WW domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IHFN. Eukaryota.
ENOG41103DM. LUCA.
GeneTreeiENSGT00390000002226.
HOGENOMiHOG000015395.
HOVERGENiHBG058016.
InParanoidiQ6ZWJ1.
KOiK15302.
OMAiHPVMSVL.
OrthoDBiEOG7B5WVP.
PhylomeDBiQ6ZWJ1.
TreeFamiTF331084.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZWJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKNTSTVVS PSLLEKDPAF QMITIAKETG LGLKVLGGIN RNEGPLVYIQ
60 70 80 90 100
EIIPGGDCYK DGRLKPGDQL VSVNKESMIG VSFEEAKSII TGAKLRLESA
110 120 130 140 150
WEIAFIRQKS DNIQPENLSC TSLIEASGEY GPQASTLSLF SSPPEILIPK
160 170 180 190 200
TSSTPKTNND ILSSCEIKTG YNKTVQIPIT SENSTVGLSN TDVASAWTEN
210 220 230 240 250
YGLQEKISLN PSVRFKAEKL EMALNYLGIQ PTKEQHQALR QQVQADSKGT
260 270 280 290 300
VSFGDFVQVA RNLFCLQLDE VNVGAHEISN ILDSQLLPCD SSEADEMERL
310 320 330 340 350
KCERDDALKE VNTLKEKLLE SDKQRKQLTE ELQNVKQEAK AVVEETRALR
360 370 380 390 400
SRIHLAEAAQ RQAHGMEMDY EEVIRLLEAK ITELKAQLAD YSDQNKESVQ
410 420 430 440 450
DLKKRIMVLD CQLRKSEMAR KTFEASTEKL LHFVEAIQEV FSDNSTPLSN
460 470 480 490 500
LSERRAVLAS QTSLTPLGRN GRSIPATLAL ESKELVKSVR ALLDMDCLPY
510 520 530 540 550
GWEEAYTADG IKYFINHVTQ TTSWIHPVMS VLNLSRSEEN EEDCSRELPN

QKS
Length:553
Mass (Da):61,662
Last modified:May 18, 2010 - v2
Checksum:i86CCAFD0D3967DD7
GO
Isoform 2 (identifier: Q6ZWJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     95-95: L → LST
     316-320: EKLLE → WPGQN
     321-553: Missing.

Show »
Length:245
Mass (Da):26,974
Checksum:i4E92DDBBFD27DF79
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921G → R.1 Publication
Corresponds to variant rs1156287 [ dbSNP | Ensembl ].
VAR_063103
Natural varianti120 – 1201C → R.
Corresponds to variant rs34870302 [ dbSNP | Ensembl ].
VAR_056999

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7777Missing in isoform 2. 1 PublicationVSP_017175Add
BLAST
Alternative sequencei95 – 951L → LST in isoform 2. 1 PublicationVSP_017176
Alternative sequencei316 – 3205EKLLE → WPGQN in isoform 2. 1 PublicationVSP_017177
Alternative sequencei321 – 553233Missing in isoform 2. 1 PublicationVSP_017178Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122865 mRNA. Translation: BAC85511.1.
AC005177 Genomic DNA. No translation available.
AC007485 Genomic DNA. No translation available.
BC041485 mRNA. Translation: AAH41485.1.
CCDSiCCDS11584.2. [Q6ZWJ1-1]
RefSeqiNP_848604.3. NM_178509.5. [Q6ZWJ1-1]
UniGeneiHs.35199.

Genome annotation databases

EnsembliENST00000376352; ENSP00000365530; ENSG00000166263. [Q6ZWJ1-1]
ENST00000398391; ENSP00000381427; ENSG00000166263. [Q6ZWJ1-2]
GeneIDi252983.
KEGGihsa:252983.
UCSCiuc002iuf.2. human. [Q6ZWJ1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122865 mRNA. Translation: BAC85511.1.
AC005177 Genomic DNA. No translation available.
AC007485 Genomic DNA. No translation available.
BC041485 mRNA. Translation: AAH41485.1.
CCDSiCCDS11584.2. [Q6ZWJ1-1]
RefSeqiNP_848604.3. NM_178509.5. [Q6ZWJ1-1]
UniGeneiHs.35199.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YSGNMR-A498-530[»]
ProteinModelPortaliQ6ZWJ1.
SMRiQ6ZWJ1. Positions 14-107, 498-530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128947. 26 interactions.
IntActiQ6ZWJ1. 9 interactions.
MINTiMINT-2879195.
STRINGi9606.ENSP00000365530.

PTM databases

iPTMnetiQ6ZWJ1.
PhosphoSiteiQ6ZWJ1.

Polymorphism and mutation databases

BioMutaiSTXBP4.
DMDMi296452915.

Proteomic databases

EPDiQ6ZWJ1.
MaxQBiQ6ZWJ1.
PaxDbiQ6ZWJ1.
PRIDEiQ6ZWJ1.

Protocols and materials databases

DNASUi252983.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376352; ENSP00000365530; ENSG00000166263. [Q6ZWJ1-1]
ENST00000398391; ENSP00000381427; ENSG00000166263. [Q6ZWJ1-2]
GeneIDi252983.
KEGGihsa:252983.
UCSCiuc002iuf.2. human. [Q6ZWJ1-1]

Organism-specific databases

CTDi252983.
GeneCardsiSTXBP4.
H-InvDBHIX0027147.
HGNCiHGNC:19694. STXBP4.
HPAiCAB007760.
HPA022138.
HPA022888.
HPA024227.
MIMi610415. gene.
neXtProtiNX_Q6ZWJ1.
PharmGKBiPA134962267.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHFN. Eukaryota.
ENOG41103DM. LUCA.
GeneTreeiENSGT00390000002226.
HOGENOMiHOG000015395.
HOVERGENiHBG058016.
InParanoidiQ6ZWJ1.
KOiK15302.
OMAiHPVMSVL.
OrthoDBiEOG7B5WVP.
PhylomeDBiQ6ZWJ1.
TreeFamiTF331084.

Enzyme and pathway databases

SignaLinkiQ6ZWJ1.

Miscellaneous databases

EvolutionaryTraceiQ6ZWJ1.
GenomeRNAii252983.
NextBioi92034.
PROiQ6ZWJ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZWJ1.
CleanExiHS_STXBP4.
ExpressionAtlasiQ6ZWJ1. baseline and differential.
GenevisibleiQ6ZWJ1. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-92.
    Tissue: Tongue.
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Eye.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Solution structure of the WW domain from the human syntaxin-binding protein 4."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 498-530.

Entry informationi

Entry nameiSTXB4_HUMAN
AccessioniPrimary (citable) accession number: Q6ZWJ1
Secondary accession number(s): Q8IVZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: May 18, 2010
Last modified: March 16, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.