Reviewed, UniProtKB/Swiss-Prot Q6ZW76 (ANKS3_HUMAN)

Names and origin

Protein names

Recommended name:
    Ankyrin repeat and SAM domain-containing protein 3

Gene names

Name:	ANKS3
Synonyms:	KIAA1977

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Contains 6 ANK repeats. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ZW76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZW76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 656	656	Ankyrin repeat and SAM domain-containing protein 3		PRO_0000230777
Regions
	Repeat	34 – 64	31	ANK 1
	Repeat	68 – 97	30	ANK 2
	Repeat	101 – 130	30	ANK 3
	Repeat	134 – 163	30	ANK 4
	Repeat	168 – 197	30	ANK 5
	Repeat	201 – 220	20	ANK 6
	Domain	425 – 488	64	SAM
	Coiled coil	501 – 526	26	Potential
	Compositional bias	329 – 382	54	Ser-rich
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.3
	Modified residue	5	1	Phosphoserine Ref.3
	Modified residue	9	1	Phosphoserine Ref.3
	Modified residue	320	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1 – 73	73	Missing in isoform 2.		VSP_038183
	Natural variant	404	1	A → T: dbSNP rs863980. Ref.1		VAR_048283
	Natural variant	593	1	A → T: dbSNP rs9936675.		VAR_048284
Experimental info
	Sequence conflict	51 – 56	6	VKECVQ → HILRLF in BAB85563. Ref.2
	Sequence conflict	472	1	F → S in BAG63156. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 67BD772ED762A5BA	FASTA	656	72,038
	10 20 30 40 50 60 MSELSDEASE PELLNRSLSM WHGLGTQVSG EELDVPLDLH TAASIGQYEV VKECVQRREL 70 80 90 100 110 120 DLNKKNGGGW TPLMYASYIG HDTIVHLLLE AGVSVNVPTP EGQTPLMLAS SCGNESIAYF 130 140 150 160 170 180 LLQQGAELEM KDIQGWTALF HCTSAGHQHM VRFLLDSGAN ANVREPICGF TPLMEAAAAG 190 200 210 220 230 240 HEIIVQYFLN HGVKVDARDH SGATARMLAK QYGHMKIVAL MDTYSPSLPK SLYRSPEKYE 250 260 270 280 290 300 DLSSSDESCP APQRQRPCRK KGVSIHEGPR ALARITGIGL GGRAPRPRYE QAPPRGYVTF 310 320 330 340 350 360 NSSGENPLEE EGLCCRDVTS PINERDVESS SSSSSREEHA FCANLGPVQS SSSSEGLARA 370 380 390 400 410 420 QGLSSEASVE SNEDSDHACK SSARKQAKSY MKTKNPDSQW PPRAATDREG FLAESSPQTQ 430 440 450 460 470 480 RAPYSGPQDL AALLEQIGCL KYLQVFEEQD VDLRIFLTLT ESDLKEIGIT LFGPKRKMTS 490 500 510 520 530 540 AIARWHSSAR PPGDALELAY ADRLEAEMQE LAIQLHKRCE EVEATRGQVC QEQELRAVVE 550 560 570 580 590 600 SCLLEQDRAR EDLQARLRET WALARDAALV LDQLRACQAE LSSRVRQDQP PGAATLGLAV 610 620 630 640 650 PPADSKGWQA SLQAMSLPEL SGALEDRVRE MGQALCLVTQ SLEKLQVLNG KKWRET
	Isoform 2. Checksum: DF9E2FBF2C4F959A	FASTA	583	63,990
	10 20 30 40 50 60 MYASYIGHDT IVHLLLEAGV SVNVPTPEGQ TPLMLASSCG NESIAYFLLQ QGAELEMKDI 70 80 90 100 110 120 QGWTALFHCT SAGHQHMVRF LLDSGANANV REPICGFTPL MEAAAAGHEI IVQYFLNHGV 130 140 150 160 170 180 KVDARDHSGA TARMLAKQYG HMKIVALMDT YSPSLPKSLY RSPEKYEDLS SSDESCPAPQ 190 200 210 220 230 240 RQRPCRKKGV SIHEGPRALA RITGIGLGGR APRPRYEQAP PRGYVTFNSS GENPLEEEGL 250 260 270 280 290 300 CCRDVTSPIN ERDVESSSSS SSREEHAFCA NLGPVQSSSS SEGLARAQGL SSEASVESNE 310 320 330 340 350 360 DSDHACKSSA RKQAKSYMKT KNPDSQWPPR AATDREGFLA ESSPQTQRAP YSGPQDLAAL 370 380 390 400 410 420 LEQIGCLKYL QVFEEQDVDL RIFLTLTESD LKEIGITLFG PKRKMTSAIA RWHSSARPPG 430 440 450 460 470 480 DALELAYADR LEAEMQELAI QLHKRCEEVE ATRGQVCQEQ ELRAVVESCL LEQDRAREDL 490 500 510 520 530 540 QARLRETWAL ARDAALVLDQ LRACQAELSS RVRQDQPPGA ATLGLAVPPA DSKGWQASLQ 550 560 570 580 AMSLPELSGA LEDRVREMGQ ALCLVTQSLE KLQVLNGKKW RET

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-404. Tissue: Oesophagus and Thalamus.
[2]	"Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:319-327(2001) [PubMed: 11853319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-656. Tissue: Brain.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-5 AND SER-9, MASS SPECTROMETRY. Tissue: Epithelium.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320, MASS SPECTROMETRY. Tissue: Epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK123496 mRNA. Translation: BAC85629.1. AK301683 mRNA. Translation: BAG63156.1. AB075857 mRNA. Translation: BAB85563.1.
IPI	IPI00401758. IPI00645601.
RefSeq	NP_597707.1.
UniGene	Hs.654859
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZW76. 1 interaction.
PTM databases
PhosphoSite	Q6ZW76.
Proteomic databases
PRIDE	Q6ZW76.
Genome annotation databases
Ensembl	ENST00000304283; ENSP00000304586; ENSG00000168096; Homo sapiens. [Genome view] ENST00000446014; ENSP00000406796; ENSG00000168096; Homo sapiens. [Genome view]
GeneID	124401.
KEGG	hsa:124401.
UCSC	uc002cxj.1. human.
Organism-specific databases
CTD	124401.
GeneCards	GC16M004687.
H-InvDB	HIX0012789.
HGNC	HGNC:29422. ANKS3.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6ZW76.
HOVERGEN	Q6ZW76.
OMA	CVQRREL
OrthoDB	EOG9DNHQF
Gene expression databases
ArrayExpress	Q6ZW76.
Bgee	Q6ZW76.
CleanEx	HS_ANKS3.
Genevestigator	Q6ZW76.
GermOnline	ENSG00000168096. Homo sapiens.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF00023. Ank. 6 hits. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00248. ANK. 6 hits. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	81261.

Entry information

Entry name

ANKS3_HUMAN

Accession

Primary (citable) accession number: Q6ZW76
Secondary accession number(s): B4DWU4, Q8TF25

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2004
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.