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Q6ZVK8 (NUD18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
8-oxo-dGDP phosphatase NUDT18

EC=3.6.1.58
Alternative name(s):
2-hydroxy-dADP phosphatase
7,8-dihydro-8-oxoguanine phosphatase
MutT homolog 3
Nucleoside diphosphate-linked moiety X motif 18
Short name=Nudix motif 18
Gene names
Name:NUDT18
Synonyms:MTH3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the hydrolyzis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Hydrolyzes also 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolyzis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools. Ref.4

Catalytic activity

8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate. Ref.4

Cofactor

Manganese By similarity. Magnesium. Ref.4

Sequence similarities

Belongs to the Nudix hydrolase family.

Contains 1 nudix hydrolase domain.

Biophysicochemical properties

Kinetic parameters:

KM=10.7 µM for 8-Oxo-dGDP Ref.4

KM=11.7 µM for 8-Oxo-GDP

Vmax=212 pmol/min/µg enzyme toward 8-Oxo-dGDP (at 30 degrees Celsius)

Vmax=246 pmol/min/µg enzyme toward 8-Oxo-GDP (at 30 degrees Celsius)

pH dependence:

Optimum pH is 8.5.

Sequence caution

The sequence BAC85853.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6ZVK8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6ZVK8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.
     101-125: EPETLLSVEERGPSWVRFVFLARPT → MSVDSARAALLSTQTLPAPSPTSPP
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3233238-oxo-dGDP phosphatase NUDT18
PRO_0000324567

Regions

Domain37 – 167131Nudix hydrolase
Motif76 – 9722Nudix box

Sites

Metal binding911Magnesium or manganese By similarity
Metal binding951Magnesium or manganese By similarity

Natural variations

Alternative sequence1 – 100100Missing in isoform 2.
VSP_032276
Alternative sequence101 – 12525EPETL…LARPT → MSVDSARAALLSTQTLPAPS PTSPP in isoform 2.
VSP_032277

Experimental info

Sequence conflict1771L → P in BAB15376. Ref.1
Sequence conflict2241M → V in BAB15376. Ref.1
Sequence conflict2241M → V in BAC85853. Ref.1

Secondary structure

......................... 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 6, 2013. Version 3.
Checksum: 8162BA4BDEC4374D

FASTA32335,501
        10         20         30         40         50         60 
MASEGLAGAL ASVLAGQGSS VHSCDSAPAG EPPAPVRLRK NVCYVVLAVF LSEQDEVLLI 

        70         80         90        100        110        120 
QEAKRECRGS WYLPAGRMEP GETIVEALQR EVKEEAGLHC EPETLLSVEE RGPSWVRFVF 

       130        140        150        160        170        180 
LARPTGGILK TSKEADAESL QAAWYPRTSL PTPLRAHDIL HLVELAAQYR QQARHPLILP 

       190        200        210        220        230        240 
QELPCDLVCQ RLVATFTSAQ TVWVLVGTVG MPHLPVTACG LDPMEQRGGM KMAVLRLLQE 

       250        260        270        280        290        300 
CLTLHHLVVE IKGLLGLQHL GRDHSDGICL NVLVTVAFRS PGIQDEPPKV RGENFSWWKV 

       310        320 
MEEDLQSQLL QRLQGSSVVP VNR 

« Hide

Isoform 2 [UniParc].

Checksum: ED9DD93D505E8709
Show »

FASTA22324,463

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Cerebellum.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[4]"Human MTH3 (NUDT18) protein hydrolyzes oxidized forms of guanosine and deoxyguanosine diphosphates: comparison with MTH1 and MTH2."
Takagi Y., Setoyama D., Ito R., Kamiya H., Yamagata Y., Sekiguchi M.
J. Biol. Chem. 287:21541-21549(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, FUNCTION.
[5]"Crystal structure of the nudix domain of human nudt18."
Structural genomics consortium (SGC)
Submitted (MAR-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 26-179.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK026147 mRNA. Translation: BAB15376.1.
AK124446 mRNA. Translation: BAC85853.1. Different initiation.
BC016902 mRNA. Translation: AAH16902.1.
RefSeqNP_079091.3. NM_024815.3.
UniGeneHs.527101.
Hs.745387.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GG6X-ray2.10A26-179[»]
4HVYX-ray1.46A26-179[»]
ProteinModelPortalQ6ZVK8.
SMRQ6ZVK8. Positions 15-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122961. 34 interactions.
IntActQ6ZVK8. 32 interactions.
MINTMINT-1436358.
STRING9606.ENSP00000307852.

PTM databases

PhosphoSiteQ6ZVK8.

Polymorphism databases

DMDM460018323.

Proteomic databases

PaxDbQ6ZVK8.
PRIDEQ6ZVK8.

Protocols and materials databases

DNASU79873.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309188; ENSP00000307852; ENSG00000173566. [Q6ZVK8-1]
GeneID79873.
KEGGhsa:79873.
UCSCuc003xaq.1. human. [Q6ZVK8-1]

Organism-specific databases

CTD79873.
GeneCardsGC08M022021.
HGNCHGNC:26194. NUDT18.
HPAHPA028581.
neXtProtNX_Q6ZVK8.
PharmGKBPA142671238.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1051.
HOGENOMHOG000035136.
HOVERGENHBG108203.
InParanoidQ6ZVK8.
KOK17817.
OMAGFTPMEQ.
TreeFamTF106355.

Enzyme and pathway databases

BioCycMetaCyc:ENSG00000173566-MONOMER.
ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressQ6ZVK8.
BgeeQ6ZVK8.
CleanExHS_NUDT18.
GenevestigatorQ6ZVK8.

Family and domain databases

Gene3D3.90.79.10. 1 hit.
InterProIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSPR00502. NUDIXFAMILY.
SUPFAMSSF55811. SSF55811. 1 hit.
PROSITEPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6ZVK8.
GenomeRNAi79873.
NextBio69645.
PROQ6ZVK8.

Entry information

Entry nameNUD18_HUMAN
AccessionPrimary (citable) accession number: Q6ZVK8
Secondary accession number(s): Q8IZ75, Q9H687
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 6, 2013
Last modified: April 16, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM