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Protein

8-oxo-dGDP phosphatase NUDT18

Gene

NUDT18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the hydrolyzis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Hydrolyzes also 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolyzis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools.1 Publication

Catalytic activityi

8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate.1 Publication

Cofactori

Mn2+By similarity1 Publication, Mg2+By similarity1 Publication

Kineticsi

  1. KM=10.7 µM for 8-Oxo-dGDP1 Publication
  2. KM=11.7 µM for 8-Oxo-GDP1 Publication
  1. Vmax=212 pmol/min/µg enzyme toward 8-Oxo-dGDP (at 30 degrees Celsius)1 Publication
  2. Vmax=246 pmol/min/µg enzyme toward 8-Oxo-GDP (at 30 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi91Magnesium or manganeseBy similarity1
Metal bindingi95Magnesium or manganeseBy similarity1

GO - Molecular functioni

  • 8-hydroxy-dADP phosphatase activity Source: UniProtKB
  • 8-oxo-dGDP phosphatase activity Source: UniProtKB
  • 8-oxo-GDP phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB

GO - Biological processi

  • dADP catabolic process Source: UniProtKB
  • dGDP catabolic process Source: UniProtKB
  • GDP catabolic process Source: UniProtKB
  • nucleobase-containing small molecule catabolic process Source: Reactome

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000173566-MONOMER
ReactomeiR-HSA-2393930 Phosphate bond hydrolysis by NUDT proteins
SABIO-RKiQ6ZVK8

Names & Taxonomyi

Protein namesi
Recommended name:
8-oxo-dGDP phosphatase NUDT18 (EC:3.6.1.58)
Alternative name(s):
2-hydroxy-dADP phosphatase
7,8-dihydro-8-oxoguanine phosphatase
MutT homolog 3
Nucleoside diphosphate-linked moiety X motif 18
Short name:
Nudix motif 18
Gene namesi
Name:NUDT18
Synonyms:MTH3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000275074.1
HGNCiHGNC:26194 NUDT18
MIMi615791 gene
neXtProtiNX_Q6ZVK8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000275074
PharmGKBiPA142671238

Polymorphism and mutation databases

BioMutaiNUDT18
DMDMi460018323

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245671 – 3238-oxo-dGDP phosphatase NUDT18Add BLAST323

Proteomic databases

MaxQBiQ6ZVK8
PaxDbiQ6ZVK8
PeptideAtlasiQ6ZVK8
PRIDEiQ6ZVK8

PTM databases

iPTMnetiQ6ZVK8
PhosphoSitePlusiQ6ZVK8

Expressioni

Gene expression databases

BgeeiENSG00000275074
CleanExiHS_NUDT18
ExpressionAtlasiQ6ZVK8 baseline and differential
GenevisibleiQ6ZVK8 HS

Organism-specific databases

HPAiHPA028581
HPA030125

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi122961, 42 interactors
IntActiQ6ZVK8, 43 interactors
MINTiQ6ZVK8
STRINGi9606.ENSP00000307852

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi43 – 49Combined sources7
Beta strandi55 – 61Combined sources7
Helixi65 – 67Combined sources3
Beta strandi74 – 77Combined sources4
Helixi84 – 96Combined sources13
Beta strandi98 – 112Combined sources15
Beta strandi115 – 127Combined sources13
Helixi132 – 134Combined sources3
Beta strandi136 – 138Combined sources3
Beta strandi140 – 146Combined sources7
Beta strandi152 – 155Combined sources4
Helixi158 – 172Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GG6X-ray2.10A26-179[»]
4HVYX-ray1.46A26-179[»]
ProteinModelPortaliQ6ZVK8
SMRiQ6ZVK8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6ZVK8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 167Nudix hydrolasePROSITE-ProRule annotationAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 97Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410IN6N Eukaryota
ENOG41102IB LUCA
GeneTreeiENSGT00390000002931
HOGENOMiHOG000035136
HOVERGENiHBG108203
InParanoidiQ6ZVK8
KOiK17817
OMAiHLPITAC
OrthoDBiEOG091G0XZN
PhylomeDBiQ6ZVK8
TreeFamiTF106355

Family and domain databases

InterProiView protein in InterPro
IPR020476 Nudix_hydrolase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
PRINTSiPR00502 NUDIXFAMILY
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZVK8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASEGLAGAL ASVLAGQGSS VHSCDSAPAG EPPAPVRLRK NVCYVVLAVF
60 70 80 90 100
LSEQDEVLLI QEAKRECRGS WYLPAGRMEP GETIVEALQR EVKEEAGLHC
110 120 130 140 150
EPETLLSVEE RGPSWVRFVF LARPTGGILK TSKEADAESL QAAWYPRTSL
160 170 180 190 200
PTPLRAHDIL HLVELAAQYR QQARHPLILP QELPCDLVCQ RLVATFTSAQ
210 220 230 240 250
TVWVLVGTVG MPHLPVTACG LDPMEQRGGM KMAVLRLLQE CLTLHHLVVE
260 270 280 290 300
IKGLLGLQHL GRDHSDGICL NVLVTVAFRS PGIQDEPPKV RGENFSWWKV
310 320
MEEDLQSQLL QRLQGSSVVP VNR
Length:323
Mass (Da):35,501
Last modified:March 6, 2013 - v3
Checksum:i8162BA4BDEC4374D
GO
Isoform 2 (identifier: Q6ZVK8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.
     101-125: EPETLLSVEERGPSWVRFVFLARPT → MSVDSARAALLSTQTLPAPSPTSPP

Note: No experimental confirmation available.
Show »
Length:223
Mass (Da):24,463
Checksum:iED9DD93D505E8709
GO

Sequence cautioni

The sequence BAC85853 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177L → P in BAB15376 (PubMed:14702039).Curated1
Sequence conflicti224M → V in BAB15376 (PubMed:14702039).Curated1
Sequence conflicti224M → V in BAC85853 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0322761 – 100Missing in isoform 2. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_032277101 – 125EPETL…LARPT → MSVDSARAALLSTQTLPAPS PTSPP in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026147 mRNA Translation: BAB15376.1
AK124446 mRNA Translation: BAC85853.1 Different initiation.
BC016902 mRNA Translation: AAH16902.1
CCDSiCCDS75706.1 [Q6ZVK8-1]
RefSeqiNP_079091.3, NM_024815.3 [Q6ZVK8-1]
UniGeneiHs.527101
Hs.745387

Genome annotation databases

EnsembliENST00000611621; ENSP00000480722; ENSG00000275074 [Q6ZVK8-1]
GeneIDi79873
KEGGihsa:79873
UCSCiuc033beu.2 human [Q6ZVK8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUD18_HUMAN
AccessioniPrimary (citable) accession number: Q6ZVK8
Secondary accession number(s): Q8IZ75, Q9H687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 6, 2013
Last modified: February 28, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health