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Protein

DENN domain-containing protein 5B

Gene

DENND5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.1 Publication

GO - Molecular functioni

  • calcium channel activity Source: GO_Central
  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 5B
Alternative name(s):
Rab6IP1-like protein
Gene namesi
Name:DENND5B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:28338. DENND5B.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei916 – 936HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000170456.
PharmGKBiPA164718788.

Polymorphism and mutation databases

BioMutaiDENND5B.
DMDMi182676608.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003265312 – 1274DENN domain-containing protein 5BAdd BLAST1273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei49PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei822PhosphoserineCombined sources1
Modified residuei1076PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6ZUT9.
PaxDbiQ6ZUT9.
PeptideAtlasiQ6ZUT9.
PRIDEiQ6ZUT9.

PTM databases

iPTMnetiQ6ZUT9.
PhosphoSitePlusiQ6ZUT9.

Expressioni

Gene expression databases

BgeeiENSG00000170456.
CleanExiHS_DENND5B.
ExpressionAtlasiQ6ZUT9. baseline and differential.
GenevisibleiQ6ZUT9. HS.

Organism-specific databases

HPAiHPA038865.

Interactioni

Protein-protein interaction databases

BioGridi127761. 3 interactors.
STRINGi9606.ENSP00000373734.

Structurei

3D structure databases

ProteinModelPortaliQ6ZUT9.
SMRiQ6ZUT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 120UDENNAdd BLAST71
Domaini187 – 375DENNPROSITE-ProRule annotationAdd BLAST189
Domaini498 – 574dDENNPROSITE-ProRule annotationAdd BLAST77
Domaini772 – 932RUN 1PROSITE-ProRule annotationAdd BLAST161
Domaini936 – 1044PLATPROSITE-ProRule annotationAdd BLAST109
Domaini1118 – 1267RUN 2PROSITE-ProRule annotationAdd BLAST150

Sequence similaritiesi

Belongs to the RAB6IP1 family.Curated
Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation
Contains 2 RUN domains.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOVERGENiHBG059906.
InParanoidiQ6ZUT9.
KOiK20164.
PhylomeDBiQ6ZUT9.
TreeFamiTF313237.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZUT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGSCAAPGP GSGSSPAACR FAHYFVLCGI DADSGLEPDE LAGENFDQSP
60 70 80 90 100
LRRTFKSKVL AHYPQNIEWN PFDQDAVNML CMPKGLSFRT QTDNKDPQFH
110 120 130 140 150
SFIITREDGS RTYGFVLTFY EEVTSKQICT AMQTLYQMHN AEHYSSVYAS
160 170 180 190 200
SSCSMDSLAS SLDEGDTTSL LKLQRYNSYD ISRDTLYVSK SICLITPLPF
210 220 230 240 250
MQACKKFLIQ LYKAVTSQQP PPLPLESYIH NILYEVPLPP PGRSLKFYGV
260 270 280 290 300
YEPVICQRPG PSELPLSDYP LREAFELLGL ENLVQVFTCV LLEMQILLYS
310 320 330 340 350
QDYQRLMTVA EGITTLLFPF QWQHVYVPIL PASLLHFLDA PVPYLMGLQS
360 370 380 390 400
KEGTDRSKLE LPQEANLCFV DIDNHFIELP EEFPQFPNKV DFIQELSEVL
410 420 430 440 450
VQFGIPPEGS LHCSESTSKL KNMVLKDLVN DKKNGNVCTN NISMYELLKG
460 470 480 490 500
NETIARLQAL AKRTGVAVEK MDLSASLGEK DKDLKLHCEE AELRDYQLNV
510 520 530 540 550
QLREVFANRF TQMFADYEAF VIQTAQDMES WLTNREQMQN FDKASFLSDQ
560 570 580 590 600
PEPYLPFLSR FIETQMFATF IDNKIMSQWE EKDPLLRVFD TRIDKIRLYN
610 620 630 640 650
VRAPTLRTSI YQKCSTLKEA AQSIEQRLMK MDHTAIHPHL LDMKIGQGKY
660 670 680 690 700
EQGFFPKLQS DVLATGPTSN NRWVSRSATA QRRKERLRQH SEHVGLDNDL
710 720 730 740 750
REKYMQEARS LGKNLRQPKL SDLSPAVIAQ TNCKFVEGLL KECRMKTKRM
760 770 780 790 800
LVEKMGHEAV ELGHGEANIT GLEENTLIAS LCDLLERIWS HGLQVKQGKS
810 820 830 840 850
ALWSHLIQFQ DREEKQEHLA ESPVALGPER RKSDSGVMLP TLRVSLIQDM
860 870 880 890 900
RHIQNMSEIK TDVGRARAWI RLSLEKKLLS QHLKQLLSNQ PLTKKLYKRY
910 920 930 940 950
AFLRCEEERE QFLYHLLSLN AVDYFCFTSV FTTIMIPYRS VIIPIKKLSN
960 970 980 990 1000
AIITSNPWIC VSGELGDTGV MQIPKNLLEM TFECQNLGKL TTVQIGHDNS
1010 1020 1030 1040 1050
GLLAKWLVDC VMVRNEITGH TYRFPCGRWL GKGIDDGSLE RILIGELMTS
1060 1070 1080 1090 1100
ASDEDLVKQC RTPPQQKSPT TARRLSITSL TGKNNKPNAG QIQEGIGEAV
1110 1120 1130 1140 1150
NNIVKHFHKP EKERGSLTVL LCGENGLVAA LEQVFHHGFK SARIFHKNVF
1160 1170 1180 1190 1200
IWDFIEKVVA YFETTDQILD NEDDVLIQKS SCKTFCHYVN AINTAPRNIG
1210 1220 1230 1240 1250
KDGKFQILVC LGTRDRLLPQ WIPLLAECPA ITRMYEESAL LRDRMTVNSL
1260 1270
IRILQTIQDF TIVLEGSLIK GVDV
Length:1,274
Mass (Da):145,020
Last modified:April 8, 2008 - v2
Checksum:i712F41E50EEE910A
GO
Isoform 2 (identifier: Q6ZUT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: MSGSCAAPGP...DSGLEPDELA → MGTHHHAQLI...KSQGAANTTS

Show »
Length:1,309
Mass (Da):149,335
Checksum:iCEA06EB33B0A186A
GO
Isoform 3 (identifier: Q6ZUT9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.
     544-585: ASFLSDQPEP...MSQWEEKDPL → VKRTIVFLLW...PYIIDFVNLF
     586-1274: Missing.

Show »
Length:507
Mass (Da):58,284
Checksum:iF8F36E3737B55C1A
GO
Isoform 4 (identifier: Q6ZUT9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-42: A → AVLYQWLEADRHGKSQGAANTTS
     703-716: KYMQEARSLGKNLR → VCGLPSWGASQQAP
     717-1274: Missing.

Show »
Length:738
Mass (Da):84,001
Checksum:iD76AB5126C9520EE
GO

Sequence cautioni

The sequence BAC04583 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti434N → D in AAH68580 (PubMed:15489334).Curated1
Sequence conflicti480K → R in BAC04583 (PubMed:14702039).Curated1
Sequence conflicti538M → T in BAC04583 (PubMed:14702039).Curated1
Sequence conflicti1144I → T in BAC04583 (PubMed:14702039).Curated1
Sequence conflicti1149V → A in BAC04583 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04007652R → K.1 PublicationCorresponds to variant rs4930979dbSNPEnsembl.1
Natural variantiVAR_040077487H → N.2 PublicationsCorresponds to variant rs1056320dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0326721 – 78Missing in isoform 3. 1 PublicationAdd BLAST78
Alternative sequenceiVSP_0326731 – 42MSGSC…PDELA → MGTHHHAQLIFVFLVEMRFH RVDQAGLELLTSGNSPASAS RSAEITVVSQHAQPGFLYQW LEADRHGKSQGAANTTS in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_03267442A → AVLYQWLEADRHGKSQGAAN TTS in isoform 4. 1 Publication1
Alternative sequenceiVSP_032675544 – 585ASFLS…EKDPL → VKRTIVFLLWLLVALLLILT FLNFLFFCAYAYPYIIDFVN LF in isoform 3. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_032676586 – 1274Missing in isoform 3. 1 PublicationAdd BLAST689
Alternative sequenceiVSP_032677703 – 716KYMQE…GKNLR → VCGLPSWGASQQAP in isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_032678717 – 1274Missing in isoform 4. 1 PublicationAdd BLAST558

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095598 mRNA. Translation: BAC04583.1. Different initiation.
AK125323 mRNA. Translation: BAC86129.1.
AC022080 Genomic DNA. No translation available.
AC068792 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88553.1.
BC020855 mRNA. Translation: AAH20855.1.
BC068580 mRNA. Translation: AAH68580.1.
AB209341 mRNA. Translation: BAD92578.1.
BX537924 mRNA. Translation: CAD97905.1.
CR749639 mRNA. Translation: CAH18433.1.
CCDSiCCDS44857.1. [Q6ZUT9-1]
CCDS76542.1. [Q6ZUT9-2]
RefSeqiNP_001295268.1. NM_001308339.1.
NP_659410.3. NM_144973.3. [Q6ZUT9-1]
UniGeneiHs.118166.

Genome annotation databases

EnsembliENST00000354285; ENSP00000346238; ENSG00000170456. [Q6ZUT9-4]
ENST00000389082; ENSP00000373734; ENSG00000170456. [Q6ZUT9-1]
GeneIDi160518.
KEGGihsa:160518.
UCSCiuc001rki.2. human. [Q6ZUT9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095598 mRNA. Translation: BAC04583.1. Different initiation.
AK125323 mRNA. Translation: BAC86129.1.
AC022080 Genomic DNA. No translation available.
AC068792 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88553.1.
BC020855 mRNA. Translation: AAH20855.1.
BC068580 mRNA. Translation: AAH68580.1.
AB209341 mRNA. Translation: BAD92578.1.
BX537924 mRNA. Translation: CAD97905.1.
CR749639 mRNA. Translation: CAH18433.1.
CCDSiCCDS44857.1. [Q6ZUT9-1]
CCDS76542.1. [Q6ZUT9-2]
RefSeqiNP_001295268.1. NM_001308339.1.
NP_659410.3. NM_144973.3. [Q6ZUT9-1]
UniGeneiHs.118166.

3D structure databases

ProteinModelPortaliQ6ZUT9.
SMRiQ6ZUT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127761. 3 interactors.
STRINGi9606.ENSP00000373734.

PTM databases

iPTMnetiQ6ZUT9.
PhosphoSitePlusiQ6ZUT9.

Polymorphism and mutation databases

BioMutaiDENND5B.
DMDMi182676608.

Proteomic databases

MaxQBiQ6ZUT9.
PaxDbiQ6ZUT9.
PeptideAtlasiQ6ZUT9.
PRIDEiQ6ZUT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354285; ENSP00000346238; ENSG00000170456. [Q6ZUT9-4]
ENST00000389082; ENSP00000373734; ENSG00000170456. [Q6ZUT9-1]
GeneIDi160518.
KEGGihsa:160518.
UCSCiuc001rki.2. human. [Q6ZUT9-1]

Organism-specific databases

CTDi160518.
GeneCardsiDENND5B.
H-InvDBHIX0010527.
HGNCiHGNC:28338. DENND5B.
HPAiHPA038865.
neXtProtiNX_Q6ZUT9.
OpenTargetsiENSG00000170456.
PharmGKBiPA164718788.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOVERGENiHBG059906.
InParanoidiQ6ZUT9.
KOiK20164.
PhylomeDBiQ6ZUT9.
TreeFamiTF313237.

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiDENND5B. human.
GenomeRNAii160518.
PROiQ6ZUT9.

Gene expression databases

BgeeiENSG00000170456.
CleanExiHS_DENND5B.
ExpressionAtlasiQ6ZUT9. baseline and differential.
GenevisibleiQ6ZUT9. HS.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEN5B_HUMAN
AccessioniPrimary (citable) accession number: Q6ZUT9
Secondary accession number(s): B5ME75
, Q59FW8, Q68CZ7, Q6NUJ0, Q7Z3F9, Q8N973, Q8WUC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 30, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.