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Q6ZUM4 (RHG27_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
SH3 domain-containing protein 20
Gene names
Name:ARHGAP27
Synonyms:CAMGAP1, SH3D20
ORF Names:PP905
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 By similarity.

Subunit structure

Interacts with SH3KBP1/CIN85 By similarity.

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer. Ref.5

Sequence similarities

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Contains 3 WW domains.

Caution

According to HGNC, ARHGAP27 and SH3D20 are 2 separate genes, corresponding to isoform 2 and isoform 4, respectively. However, a rat transcript and paralog proteins with a similar domain structure suggest the existence of a single gene encoding for a protein of 889 residues as displayed here.

Sequence caution

The sequence AAI01389.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI01390.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI01391.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6ZUM4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6ZUM4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6ZUM4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     416-442: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6ZUM4-4)

Also known as: SH3D20;

The sequence of this isoform differs from the canonical sequence as follows:
     221-263: DDPPEPVYAN...THTDAGTGRP → PPRALGRGGG...RRPPLSEDFG
     264-889: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 889889Rho GTPase-activating protein 27
PRO_0000317578

Regions

Domain8 – 6962SH3
Domain246 – 28035WW 1
Domain299 – 33335WW 2
Domain411 – 44434WW 3
Domain496 – 612117PH
Domain697 – 886190Rho-GAP

Amino acid modifications

Modified residue2161Phosphoserine By similarity

Natural variations

Alternative sequence1 – 341341Missing in isoform 2.
VSP_031053
Alternative sequence221 – 26343DDPPE…GTGRP → PPRALGRGGGWRARDRARTE PGRKETRSAQRRARRPPLSE DFG in isoform 4.
VSP_031054
Alternative sequence264 – 889626Missing in isoform 4.
VSP_031055
Alternative sequence416 – 44227Missing in isoform 3.
VSP_031056
Natural variant8891H → Q. Ref.4
Corresponds to variant rs34793644 [ dbSNP | Ensembl ].
VAR_038551

Experimental info

Sequence conflict411S → N in AAG23796. Ref.1
Sequence conflict1621C → R in BAF83339. Ref.2
Sequence conflict2131P → R in AAH67345. Ref.4

Secondary structure

..................... 889
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 13, 2010. Version 3.
Checksum: E341CCC4D012DA4C

FASTA88998,396
        10         20         30         40         50         60 
MAADVVGDVY VLVEHPFEYT GKDGRRVAIR PNERYRLLRR STEHWWHVRR EPGGRPFYLP 

        70         80         90        100        110        120 
AQYVRELPAL GNPAAAAPPG PHPSPAAPEP LAYDYRFVSA AATAGPDGAP EESGGRASSL 

       130        140        150        160        170        180 
CGPAQRGAAT QRSSLAPGLP ACLYLRPAAP VRPAQSLNDL ACAAVSPPAG LLGSSGSFKA 

       190        200        210        220        230        240 
CSVAGSWVCP RPLARSDSEN VYEVIQDLHV PPPEESAEQV DDPPEPVYAN IERQPRATSP 

       250        260        270        280        290        300 
GAAAAPLPSP VWETHTDAGT GRPYYYNPDT GVTTWESPFE AAEGAASPAT SPASVDSHVS 

       310        320        330        340        350        360 
LETEWGQYWD EESRRVFFYN PLTGETAWED EAENEPEEEL EMQPGLSPGS PGDPRPPTPE 

       370        380        390        400        410        420 
TDYPESLTSY PEEDYSPVGS FGEPGPTSPL TTPPGWSCHV SQDKQMLYTN HFTQEQWVRL 

       430        440        450        460        470        480 
EDPHGKPYFY NPEDSSVRWE LPQVPVPAPR SIHKSSQDGD TPAQASPPEE KVPAELDEVG 

       490        500        510        520        530        540 
SWEEVSPATA AVRTKTLDKA GVLHRTKTAD KGKRLRKKHW SASWTVLEGG VLTFFKDSKT 

       550        560        570        580        590        600 
SAAGGLRQPS KFSTPEYTVE LRGATLSWAP KDKSSRKNVL ELRSRDGSEY LIQHDSEAII 

       610        620        630        640        650        660 
STWHKAIAQG IQELSAELPP EESESSRVDF GSSERLGSWQ EKEEDARPNA AAPALGPVGL 

       670        680        690        700        710        720 
ESDLSKVRHK LRKFLQRRPT LQSLREKGYI KDQVFGCALA ALCERERSRV PRFVQQCIRA 

       730        740        750        760        770        780 
VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA LKLFFRELPE 

       790        800        810        820        830        840 
PLFPFSHFRQ FIAAIKLQDQ ARRSRCVRDL VRSLPAPNHD TLRMLFQHLC RVIEHGEQNR 

       850        860        870        880 
MSVQSVAIVF GPTLLRPEVE ETSMPMTMVF QNQVVELILQ QCADIFPPH 

« Hide

Isoform 2 [UniParc].

Checksum: 4E0EE2190C9F20E8
Show »

FASTA54861,733
Isoform 3 [UniParc].

Checksum: F207CD53BDAE7EAD
Show »

FASTA86295,068
Isoform 4 (SH3D20) [UniParc].

Checksum: 5731E04A9F89B4D4
Show »

FASTA26328,192

References

« Hide 'large scale' references
[1]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Embryo and Prostate.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 270-889 (ISOFORMS 1 AND 3), VARIANT GLN-889.
Tissue: Pancreas.
[5]"Identification and characterization of ARHGAP27 gene in silico."
Katoh Y., Katoh M.
Int. J. Mol. Med. 14:943-947(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF258593 mRNA. Translation: AAG23796.1.
AK125535 mRNA. Translation: BAC86196.1.
AK290650 mRNA. Translation: BAF83339.1.
AC003070 Genomic DNA. No translation available.
AC091132 Genomic DNA. No translation available.
BC067345 mRNA. Translation: AAH67345.1.
BC101388 mRNA. Translation: AAI01389.1. Different initiation.
BC101389 mRNA. Translation: AAI01390.1. Different initiation.
BC101390 mRNA. Translation: AAI01391.1. Different initiation.
BC101391 mRNA. Translation: AAI01392.3.
CCDSCCDS11498.1. [Q6ZUM4-2]
CCDS32670.1. [Q6ZUM4-4]
RefSeqNP_001269219.1. NM_001282290.1. [Q6ZUM4-1]
NP_777579.2. NM_174919.3. [Q6ZUM4-4]
NP_954976.1. NM_199282.2. [Q6ZUM4-2]
XP_006721808.1. XM_006721745.1. [Q6ZUM4-1]
XP_006721809.1. XM_006721746.1. [Q6ZUM4-1]
XP_006725671.1. XM_006725608.1. [Q6ZUM4-1]
XP_006725672.1. XM_006725609.1. [Q6ZUM4-1]
UniGeneHs.205326.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PP2X-ray1.42A/B491-613[»]
ProteinModelPortalQ6ZUM4.
SMRQ6ZUM4. Positions 10-67, 498-612, 678-886.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128369. 4 interactions.
IntActQ6ZUM4. 1 interaction.
MINTMINT-5006179.
STRING9606.ENSP00000366121.

PTM databases

PhosphoSiteQ6ZUM4.

Polymorphism databases

DMDM300669680.

Proteomic databases

MaxQBQ6ZUM4.
PaxDbQ6ZUM4.
PRIDEQ6ZUM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000290470; ENSP00000290470; ENSG00000159314. [Q6ZUM4-4]
ENST00000376922; ENSP00000366121; ENSG00000159314. [Q6ZUM4-2]
ENST00000428638; ENSP00000403323; ENSG00000159314. [Q6ZUM4-1]
ENST00000442348; ENSP00000409330; ENSG00000159314. [Q6ZUM4-3]
ENST00000455881; ENSP00000408235; ENSG00000159314. [Q6ZUM4-2]
ENST00000528273; ENSP00000436137; ENSG00000159314. [Q6ZUM4-4]
ENST00000528384; ENSP00000431591; ENSG00000159314.
GeneID201176.
KEGGhsa:201176.
UCSCuc002iix.3. human. [Q6ZUM4-1]
uc010dal.3. human. [Q6ZUM4-4]

Organism-specific databases

CTD201176.
GeneCardsGC17M043488.
HGNCHGNC:31813. ARHGAP27.
HPAHPA023919.
MIM610591. gene.
neXtProtNX_Q6ZUM4.
PharmGKBPA134873327.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG279563.
HOVERGENHBG005328.
OMATPEYTVD.
PhylomeDBQ6ZUM4.
TreeFamTF329345.

Enzyme and pathway databases

SignaLinkQ6ZUM4.

Gene expression databases

ArrayExpressQ6ZUM4.
BgeeQ6ZUM4.
CleanExHS_ARHGAP27.
GenevestigatorQ6ZUM4.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERPTHR23181. PTHR23181. 1 hit.
PfamPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 1 hit.
SSF51045. SSF51045. 2 hits.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi201176.
NextBio90073.
PROQ6ZUM4.
SOURCESearch...

Entry information

Entry nameRHG27_HUMAN
AccessionPrimary (citable) accession number: Q6ZUM4
Secondary accession number(s): A4FU35 expand/collapse secondary AC list , A8K3N5, C9JTF3, Q494U0, Q6NWZ8, Q8WY58
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 13, 2010
Last modified: July 9, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM