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Protein

Rho GTPase-activating protein 27

Gene

ARHGAP27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ6ZUM4.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
SH3 domain-containing protein 20
Gene namesi
Name:ARHGAP27
Synonyms:CAMGAP1, SH3D20
ORF Names:PP905
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:31813. ARHGAP27.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi201176.
OpenTargetsiENSG00000159314.
ENSG00000276836.
ENSG00000276907.
PharmGKBiPA134873327.

Polymorphism and mutation databases

BioMutaiARHGAP27.
DMDMi300669680.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175781 – 889Rho GTPase-activating protein 27Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei249PhosphoserineCombined sources1
Modified residuei347PhosphoserineBy similarity1
Modified residuei456PhosphoserineBy similarity1
Modified residuei461PhosphothreonineBy similarity1
Modified residuei466PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZUM4.
MaxQBiQ6ZUM4.
PaxDbiQ6ZUM4.
PeptideAtlasiQ6ZUM4.
PRIDEiQ6ZUM4.

PTM databases

iPTMnetiQ6ZUM4.
PhosphoSitePlusiQ6ZUM4.

Expressioni

Tissue specificityi

Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer.1 Publication

Gene expression databases

BgeeiENSG00000159314.
CleanExiHS_ARHGAP27.
ExpressionAtlasiQ6ZUM4. baseline and differential.
GenevisibleiQ6ZUM4. HS.

Organism-specific databases

HPAiHPA023919.
HPA053053.

Interactioni

Subunit structurei

Interacts with SH3KBP1/CIN85.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi128369. 3 interactors.
IntActiQ6ZUM4. 1 interactor.
MINTiMINT-5006179.
STRINGi9606.ENSP00000366121.

Structurei

Secondary structure

1889
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi499 – 510Combined sources12
Beta strandi521 – 528Combined sources8
Beta strandi531 – 536Combined sources6
Helixi549 – 552Combined sources4
Beta strandi554 – 560Combined sources7
Beta strandi565 – 568Combined sources4
Helixi571 – 573Combined sources3
Beta strandi575 – 583Combined sources9
Beta strandi589 – 593Combined sources5
Helixi597 – 611Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PP2X-ray1.42A/B491-613[»]
ProteinModelPortaliQ6ZUM4.
SMRiQ6ZUM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 69SH3PROSITE-ProRule annotationAdd BLAST62
Domaini246 – 280WW 1PROSITE-ProRule annotationAdd BLAST35
Domaini299 – 333WW 2PROSITE-ProRule annotationAdd BLAST35
Domaini411 – 444WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini496 – 612PHPROSITE-ProRule annotationAdd BLAST117
Domaini697 – 886Rho-GAPPROSITE-ProRule annotationAdd BLAST190

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 3 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
KOG4269. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOVERGENiHBG005328.
InParanoidiQ6ZUM4.
KOiK20636.
OMAiNLEDGQW.
OrthoDBiEOG091G164H.
PhylomeDBiQ6ZUM4.
TreeFamiTF329345.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 2 hits.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF50998. SSF50998. 1 hit.
SSF51045. SSF51045. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZUM4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADVVGDVY VLVEHPFEYT GKDGRRVAIR PNERYRLLRR STEHWWHVRR
60 70 80 90 100
EPGGRPFYLP AQYVRELPAL GNPAAAAPPG PHPSPAAPEP LAYDYRFVSA
110 120 130 140 150
AATAGPDGAP EESGGRASSL CGPAQRGAAT QRSSLAPGLP ACLYLRPAAP
160 170 180 190 200
VRPAQSLNDL ACAAVSPPAG LLGSSGSFKA CSVAGSWVCP RPLARSDSEN
210 220 230 240 250
VYEVIQDLHV PPPEESAEQV DDPPEPVYAN IERQPRATSP GAAAAPLPSP
260 270 280 290 300
VWETHTDAGT GRPYYYNPDT GVTTWESPFE AAEGAASPAT SPASVDSHVS
310 320 330 340 350
LETEWGQYWD EESRRVFFYN PLTGETAWED EAENEPEEEL EMQPGLSPGS
360 370 380 390 400
PGDPRPPTPE TDYPESLTSY PEEDYSPVGS FGEPGPTSPL TTPPGWSCHV
410 420 430 440 450
SQDKQMLYTN HFTQEQWVRL EDPHGKPYFY NPEDSSVRWE LPQVPVPAPR
460 470 480 490 500
SIHKSSQDGD TPAQASPPEE KVPAELDEVG SWEEVSPATA AVRTKTLDKA
510 520 530 540 550
GVLHRTKTAD KGKRLRKKHW SASWTVLEGG VLTFFKDSKT SAAGGLRQPS
560 570 580 590 600
KFSTPEYTVE LRGATLSWAP KDKSSRKNVL ELRSRDGSEY LIQHDSEAII
610 620 630 640 650
STWHKAIAQG IQELSAELPP EESESSRVDF GSSERLGSWQ EKEEDARPNA
660 670 680 690 700
AAPALGPVGL ESDLSKVRHK LRKFLQRRPT LQSLREKGYI KDQVFGCALA
710 720 730 740 750
ALCERERSRV PRFVQQCIRA VEARGLDIDG LYRISGNLAT IQKLRYKVDH
760 770 780 790 800
DERLDLDDGR WEDVHVITGA LKLFFRELPE PLFPFSHFRQ FIAAIKLQDQ
810 820 830 840 850
ARRSRCVRDL VRSLPAPNHD TLRMLFQHLC RVIEHGEQNR MSVQSVAIVF
860 870 880
GPTLLRPEVE ETSMPMTMVF QNQVVELILQ QCADIFPPH
Length:889
Mass (Da):98,396
Last modified:July 13, 2010 - v3
Checksum:iE341CCC4D012DA4C
GO
Isoform 2 (identifier: Q6ZUM4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.

Note: No experimental confirmation available.
Show »
Length:548
Mass (Da):61,733
Checksum:i4E0EE2190C9F20E8
GO
Isoform 3 (identifier: Q6ZUM4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-442: Missing.

Note: No experimental confirmation available.
Show »
Length:862
Mass (Da):95,068
Checksum:iF207CD53BDAE7EAD
GO
Isoform 4 (identifier: Q6ZUM4-4) [UniParc]FASTAAdd to basket
Also known as: SH3D20

The sequence of this isoform differs from the canonical sequence as follows:
     221-263: DDPPEPVYAN...THTDAGTGRP → PPRALGRGGG...RRPPLSEDFG
     264-889: Missing.

Show »
Length:263
Mass (Da):28,192
Checksum:i5731E04A9F89B4D4
GO

Sequence cautioni

The sequence AAI01389 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI01390 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI01391 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41S → N in AAG23796 (PubMed:15498874).Curated1
Sequence conflicti162C → R in BAF83339 (PubMed:14702039).Curated1
Sequence conflicti213P → R in AAH67345 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038551889H → Q.1 PublicationCorresponds to variant rs34793644dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0310531 – 341Missing in isoform 2. 1 PublicationAdd BLAST341
Alternative sequenceiVSP_031054221 – 263DDPPE…GTGRP → PPRALGRGGGWRARDRARTE PGRKETRSAQRRARRPPLSE DFG in isoform 4. 2 PublicationsAdd BLAST43
Alternative sequenceiVSP_031055264 – 889Missing in isoform 4. 2 PublicationsAdd BLAST626
Alternative sequenceiVSP_031056416 – 442Missing in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF258593 mRNA. Translation: AAG23796.1.
AK125535 mRNA. Translation: BAC86196.1.
AK290650 mRNA. Translation: BAF83339.1.
AC003070 Genomic DNA. No translation available.
AC091132 Genomic DNA. No translation available.
BC067345 mRNA. Translation: AAH67345.1.
BC101388 mRNA. Translation: AAI01389.1. Different initiation.
BC101389 mRNA. Translation: AAI01390.1. Different initiation.
BC101390 mRNA. Translation: AAI01391.1. Different initiation.
BC101391 mRNA. Translation: AAI01392.3.
CCDSiCCDS11498.1. [Q6ZUM4-2]
CCDS32670.1. [Q6ZUM4-4]
CCDS74082.1. [Q6ZUM4-1]
RefSeqiNP_001269219.1. NM_001282290.1. [Q6ZUM4-1]
NP_777579.2. NM_174919.3. [Q6ZUM4-4]
NP_954976.1. NM_199282.2. [Q6ZUM4-2]
XP_006721808.1. XM_006721745.2. [Q6ZUM4-1]
UniGeneiHs.205326.

Genome annotation databases

EnsembliENST00000290470; ENSP00000290470; ENSG00000159314. [Q6ZUM4-4]
ENST00000376922; ENSP00000366121; ENSG00000159314. [Q6ZUM4-2]
ENST00000428638; ENSP00000403323; ENSG00000159314. [Q6ZUM4-1]
ENST00000528273; ENSP00000436137; ENSG00000159314. [Q6ZUM4-4]
ENST00000610792; ENSP00000477741; ENSG00000276907. [Q6ZUM4-1]
ENST00000611188; ENSP00000481302; ENSG00000276907. [Q6ZUM4-4]
ENST00000612916; ENSP00000480582; ENSG00000276836. [Q6ZUM4-2]
ENST00000616021; ENSP00000478738; ENSG00000276907. [Q6ZUM4-2]
ENST00000633003; ENSP00000487991; ENSG00000276907. [Q6ZUM4-4]
GeneIDi201176.
KEGGihsa:201176.
UCSCiuc002iix.4. human. [Q6ZUM4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF258593 mRNA. Translation: AAG23796.1.
AK125535 mRNA. Translation: BAC86196.1.
AK290650 mRNA. Translation: BAF83339.1.
AC003070 Genomic DNA. No translation available.
AC091132 Genomic DNA. No translation available.
BC067345 mRNA. Translation: AAH67345.1.
BC101388 mRNA. Translation: AAI01389.1. Different initiation.
BC101389 mRNA. Translation: AAI01390.1. Different initiation.
BC101390 mRNA. Translation: AAI01391.1. Different initiation.
BC101391 mRNA. Translation: AAI01392.3.
CCDSiCCDS11498.1. [Q6ZUM4-2]
CCDS32670.1. [Q6ZUM4-4]
CCDS74082.1. [Q6ZUM4-1]
RefSeqiNP_001269219.1. NM_001282290.1. [Q6ZUM4-1]
NP_777579.2. NM_174919.3. [Q6ZUM4-4]
NP_954976.1. NM_199282.2. [Q6ZUM4-2]
XP_006721808.1. XM_006721745.2. [Q6ZUM4-1]
UniGeneiHs.205326.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PP2X-ray1.42A/B491-613[»]
ProteinModelPortaliQ6ZUM4.
SMRiQ6ZUM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128369. 3 interactors.
IntActiQ6ZUM4. 1 interactor.
MINTiMINT-5006179.
STRINGi9606.ENSP00000366121.

PTM databases

iPTMnetiQ6ZUM4.
PhosphoSitePlusiQ6ZUM4.

Polymorphism and mutation databases

BioMutaiARHGAP27.
DMDMi300669680.

Proteomic databases

EPDiQ6ZUM4.
MaxQBiQ6ZUM4.
PaxDbiQ6ZUM4.
PeptideAtlasiQ6ZUM4.
PRIDEiQ6ZUM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290470; ENSP00000290470; ENSG00000159314. [Q6ZUM4-4]
ENST00000376922; ENSP00000366121; ENSG00000159314. [Q6ZUM4-2]
ENST00000428638; ENSP00000403323; ENSG00000159314. [Q6ZUM4-1]
ENST00000528273; ENSP00000436137; ENSG00000159314. [Q6ZUM4-4]
ENST00000610792; ENSP00000477741; ENSG00000276907. [Q6ZUM4-1]
ENST00000611188; ENSP00000481302; ENSG00000276907. [Q6ZUM4-4]
ENST00000612916; ENSP00000480582; ENSG00000276836. [Q6ZUM4-2]
ENST00000616021; ENSP00000478738; ENSG00000276907. [Q6ZUM4-2]
ENST00000633003; ENSP00000487991; ENSG00000276907. [Q6ZUM4-4]
GeneIDi201176.
KEGGihsa:201176.
UCSCiuc002iix.4. human. [Q6ZUM4-1]

Organism-specific databases

CTDi201176.
DisGeNETi201176.
GeneCardsiARHGAP27.
HGNCiHGNC:31813. ARHGAP27.
HPAiHPA023919.
HPA053053.
MIMi610591. gene.
neXtProtiNX_Q6ZUM4.
OpenTargetsiENSG00000159314.
ENSG00000276836.
ENSG00000276907.
PharmGKBiPA134873327.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
KOG4269. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOVERGENiHBG005328.
InParanoidiQ6ZUM4.
KOiK20636.
OMAiNLEDGQW.
OrthoDBiEOG091G164H.
PhylomeDBiQ6ZUM4.
TreeFamiTF329345.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ6ZUM4.

Miscellaneous databases

GenomeRNAii201176.
PROiQ6ZUM4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000159314.
CleanExiHS_ARHGAP27.
ExpressionAtlasiQ6ZUM4. baseline and differential.
GenevisibleiQ6ZUM4. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 2 hits.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF50998. SSF50998. 1 hit.
SSF51045. SSF51045. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG27_HUMAN
AccessioniPrimary (citable) accession number: Q6ZUM4
Secondary accession number(s): A4FU35
, A8K3N5, C9JTF3, Q494U0, Q6NWZ8, Q8WY58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 13, 2010
Last modified: November 30, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

According to HGNC, ARHGAP27 and SH3D20 are 2 separate genes, corresponding to isoform 2 and isoform 4, respectively. However, a rat transcript and paralog proteins with a similar domain structure suggest the existence of a single gene encoding for a protein of 889 residues as displayed here.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.