ID   CE128_HUMAN             Reviewed;        1094 AA.
AC   Q6ZU80; B9EK52; Q86X97; Q96ML4;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   27-MAR-2024, entry version 144.
DE   RecName: Full=Centrosomal protein of 128 kDa;
DE            Short=Cep128;
GN   Name=CEP128; Synonyms=C14orf145, C14orf61;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12508121; DOI=10.1038/nature01348;
RA   Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA   Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA   Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA   Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA   Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA   Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA   Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA   Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA   Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA   Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA   Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA   Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA   Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA   Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA   Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA   Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA   Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA   Waterston R., Hood L., Weissenbach J.;
RT   "The DNA sequence and analysis of human chromosome 14.";
RL   Nature 421:601-607(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 310-1094 (ISOFORM 2), AND
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 715-1094 (ISOFORM 1).
RC   TISSUE=Placenta, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS].
RC   TISSUE=Lymphoblast;
RX   PubMed=14654843; DOI=10.1038/nature02166;
RA   Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT   "Proteomic characterization of the human centrosome by protein correlation
RT   profiling.";
RL   Nature 426:570-574(2003).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=21399614; DOI=10.1038/emboj.2011.63;
RA   Jakobsen L., Vanselow K., Skogs M., Toyoda Y., Lundberg E., Poser I.,
RA   Falkenby L.G., Bennetzen M., Westendorf J., Nigg E.A., Uhlen M.,
RA   Hyman A.A., Andersen J.S.;
RT   "Novel asymmetrically localizing components of human centrosomes identified
RT   by complementary proteomics methods.";
RL   EMBO J. 30:1520-1535(2011).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-291; SER-331 AND
RP   SER-1061, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000269|PubMed:14654843}. Cytoplasm,
CC       cytoskeleton, microtubule organizing center, centrosome, centriole
CC       {ECO:0000269|PubMed:21399614}. Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000269|PubMed:21399614}. Note=Associates with the mother
CC       centriole.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=2;
CC         IsoId=Q6ZU80-2; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q6ZU80-1; Sequence=VSP_014752, VSP_030196;
CC       Name=3;
CC         IsoId=Q6ZU80-3; Sequence=VSP_030194, VSP_030195;
CC   -!- MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB71273.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC010072; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC022404; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC018513; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC045834; AAH45834.2; -; mRNA.
DR   EMBL; BC150610; AAI50611.1; -; mRNA.
DR   EMBL; AK056756; BAB71273.1; ALT_INIT; mRNA.
DR   EMBL; AK125925; -; NOT_ANNOTATED_CDS; mRNA.
DR   CCDS; CCDS32130.1; -. [Q6ZU80-2]
DR   RefSeq; NP_689659.2; NM_152446.3. [Q6ZU80-2]
DR   RefSeq; XP_006720119.1; XM_006720056.3.
DR   AlphaFoldDB; Q6ZU80; -.
DR   SMR; Q6ZU80; -.
DR   BioGRID; 126920; 295.
DR   IntAct; Q6ZU80; 278.
DR   MINT; Q6ZU80; -.
DR   STRING; 9606.ENSP00000451162; -.
DR   MoonDB; Q6ZU80; Predicted.
DR   iPTMnet; Q6ZU80; -.
DR   PhosphoSitePlus; Q6ZU80; -.
DR   BioMuta; CEP128; -.
DR   DMDM; 166214932; -.
DR   EPD; Q6ZU80; -.
DR   jPOST; Q6ZU80; -.
DR   MassIVE; Q6ZU80; -.
DR   MaxQB; Q6ZU80; -.
DR   PaxDb; 9606-ENSP00000451162; -.
DR   PeptideAtlas; Q6ZU80; -.
DR   ProteomicsDB; 68321; -. [Q6ZU80-2]
DR   ProteomicsDB; 68322; -. [Q6ZU80-1]
DR   ProteomicsDB; 68323; -. [Q6ZU80-3]
DR   Pumba; Q6ZU80; -.
DR   Antibodypedia; 2; 110 antibodies from 15 providers.
DR   DNASU; 145508; -.
DR   Ensembl; ENST00000216517.10; ENSP00000216517.6; ENSG00000100629.17. [Q6ZU80-3]
DR   Ensembl; ENST00000281129.7; ENSP00000281129.3; ENSG00000100629.17. [Q6ZU80-2]
DR   Ensembl; ENST00000555265.6; ENSP00000451162.1; ENSG00000100629.17. [Q6ZU80-2]
DR   GeneID; 145508; -.
DR   KEGG; hsa:145508; -.
DR   MANE-Select; ENST00000555265.6; ENSP00000451162.1; NM_152446.5; NP_689659.2.
DR   UCSC; uc001xux.3; human. [Q6ZU80-2]
DR   AGR; HGNC:20359; -.
DR   CTD; 145508; -.
DR   DisGeNET; 145508; -.
DR   GeneCards; CEP128; -.
DR   HGNC; HGNC:20359; CEP128.
DR   HPA; ENSG00000100629; Tissue enhanced (lymphoid).
DR   MalaCards; CEP128; -.
DR   neXtProt; NX_Q6ZU80; -.
DR   OpenTargets; ENSG00000100629; -.
DR   PharmGKB; PA142672283; -.
DR   VEuPathDB; HostDB:ENSG00000100629; -.
DR   eggNOG; ENOG502QRR8; Eukaryota.
DR   GeneTree; ENSGT00390000007020; -.
DR   HOGENOM; CLU_010570_0_0_1; -.
DR   InParanoid; Q6ZU80; -.
DR   OMA; HTMHQSL; -.
DR   OrthoDB; 5398234at2759; -.
DR   PhylomeDB; Q6ZU80; -.
DR   TreeFam; TF331714; -.
DR   PathwayCommons; Q6ZU80; -.
DR   SignaLink; Q6ZU80; -.
DR   BioGRID-ORCS; 145508; 8 hits in 1153 CRISPR screens.
DR   ChiTaRS; CEP128; human.
DR   GenomeRNAi; 145508; -.
DR   Pharos; Q6ZU80; Tbio.
DR   PRO; PR:Q6ZU80; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   RNAct; Q6ZU80; Protein.
DR   Bgee; ENSG00000100629; Expressed in bone marrow cell and 123 other cell types or tissues.
DR   ExpressionAtlas; Q6ZU80; baseline and differential.
DR   GO; GO:0120103; C:centriolar subdistal appendage; IDA:GO_Central.
DR   GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR   GO; GO:0005813; C:centrosome; IDA:HPA.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR   GO; GO:0031965; C:nuclear membrane; IDA:HPA.
DR   GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR   GO; GO:0008104; P:protein localization; IMP:GO_Central.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:Ensembl.
DR   GO; GO:0120316; P:sperm flagellum assembly; IEA:Ensembl.
DR   InterPro; IPR026652; CEP128.
DR   PANTHER; PTHR46657; CENTROSOMAL PROTEIN OF 128 KDA; 1.
DR   PANTHER; PTHR46657:SF1; CENTROSOMAL PROTEIN OF 128 KDA; 1.
DR   Genevisible; Q6ZU80; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..1094
FT                   /note="Centrosomal protein of 128 kDa"
FT                   /id="PRO_0000089946"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          115..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          319..345
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          987..1018
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1067..1094
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          190..827
FT                   /evidence="ECO:0000255"
FT   COILED          879..959
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..21
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        327..341
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         31
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         249
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8BI22"
FT   MOD_RES         291
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         331
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163,
FT                   ECO:0007744|PubMed:24275569"
FT   MOD_RES         1061
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VAR_SEQ         404..435
FT                   /note="NLTRELENGEKQQLQMLDRLKEIQNHFDTCEA -> HINRKCLLNLVQDLDC
FT                   KDNEILTYSLQSPLHV (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030194"
FT   VAR_SEQ         436..1094
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030195"
FT   VAR_SEQ         987
FT                   /note="D -> R (in isoform 1)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_014752"
FT   VAR_SEQ         988..1094
FT                   /note="Missing (in isoform 1)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_030196"
FT   VARIANT         16
FT                   /note="R -> L (in dbSNP:rs7160694)"
FT                   /id="VAR_037835"
FT   VARIANT         732
FT                   /note="H -> R (in dbSNP:rs327463)"
FT                   /id="VAR_037836"
FT   CONFLICT        395
FT                   /note="K -> R (in Ref. 2; AAH45834)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        973
FT                   /note="P -> A (in Ref. 3; BAB71273)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1094 AA;  128015 MW;  BDE05923E3F6B0FF CRC64;
     MAESSSESDH FRCRDRLSPW AARSTHRGTR SLPTVEVTEK VNTITSTLQD TSRNLRQVDQ
     MLGRYREYSN GQAGAIEHLK ESLEQSIDQL RSQRLLRNSG GRSISVTSLS ASDLDGGTGS
     ELHHFPPTSP LKDYGDPQGI KRMRSRTGVR FVQETDDMTQ LHGFHQSLRD LSSEQIRLGD
     DFNRELSRRS RSDAETKRAL EELTEKLNEA QKQEVVSDRV ERRLQELERE MRTERELVER
     RQDQLGLMSL QLQEALKKQE AKADEHEGAI KNKLRQTETE KNQLEQELEL SRRLLNQSEG
     SRETLLHQVE ELRTQLTKAE GDRKGLQHQV SQISKQQSNY QDEQGEDWRF RRGVEREKQD
     LEKQMSDLRV QLNFSAMASE LEEVKRCMER KDKEKAHLAS QVENLTRELE NGEKQQLQML
     DRLKEIQNHF DTCEAERKHA DLQISELTRH AEDATKQAER YLSELQQSEA LKEEAEKRRE
     DLKLKAQESI RQWKLKHKKL ERALEKQSET VDELTGKNNQ ILKEKDELKT QLYAALQQIE
     NLRKELNDVL TKRALQEEEL HSKEEKLRDI KSHQADLELE VKNSLDTIHR LESELKKQSK
     IQSQMKVEKA HLEEEIAELK KSQAQDKAKL LEMQESIKDL SAIRADLANK LAEEERAKKA
     VLKDLSDLTA QAKSRDEETA TIITQLKLER DVHQRELKDL TSSLQSVKTK HEQNIQELMK
     HFKKEKSEAE NHIRTLKAES LEEKNMAKIH RGQLEKLKSQ CDRLTEELTQ NENENKKLKL
     KYQCLKDQLE EREKHISIEE EHLRRMEEAR LQLKDQLLCL ETEQESILGV IGKEIDAACK
     TFSKDSVEKL KVFSSGPDIH YDPHRWLAES KTKLQWLCEE LKERENREKN LRHQLMLCRQ
     QLRNLTENKE SELQCLFQQI ERQEQLLDEI HREKRDLLEE TQRKDEEMGS LQDRVIALET
     STQVALDHLE SVPEKLSLLE DFKDFRDSCS SSERTDGRYS KYRVRRNSLQ HHQDDTKYRT
     KSFKGDRTFL EGSHTRGLDH SSSWQDHSRF LSSPRFSYVN SFTKRTVAPD SASNKEDATM
     NGTSSQPKKE EYGS
//