Reviewed, UniProtKB/Swiss-Prot Q6ZU65 (UBN2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubinuclein-2

Gene names

Name:	UBN2
Synonyms:	KIAA2030

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Tissue specificity	Expressed in several cell lines tested, including primary and transformed cell lines. Ref.9
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the ubinuclein family.
Sequence caution	The sequence EAL24038.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ZU65-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZU65-2)

The sequence of this isoform differs from the canonical sequence as follows:
     958-970: LSNNPQLSCSSSL → GPCLRDVSEQFGP
     971-1347: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1347	1347	Ubinuclein-2		PRO_0000295725
Regions
	Compositional bias	23 – 123	101	Pro-rich
	Compositional bias	262 – 345	84	Lys-rich
	Compositional bias	884 – 1224	341	Ser-rich
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.8
	Modified residue	430	1	Phosphothreonine Ref.7
	Modified residue	432	1	Phosphothreonine Ref.7
	Modified residue	584	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	958 – 970	13	LSNNP…CSSSL → GPCLRDVSEQFGP in isoform 2.		VSP_027021
	Alternative sequence	971 – 1347	377	Missing in isoform 2.		VSP_027022
	Natural variant	1308	1	P → A: dbSNP rs17160850.		VAR_033347

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 6B52672B860B18B5	FASTA	1,347	146,089
	10 20 30 40 50 60 MAEPRRVAFI SLSPVRRREA EYPGPEREPE YPREPPRLEP QPYREPARAE PPAPREPAPR 70 80 90 100 110 120 SDAQPPSREK PLPQREVSRA EPPMSLQREP PRPEPPPPFP PLPLQPPPPR ESASRAEQPP 130 140 150 160 170 180 RPPRETVRLE LVLKDPTDES CVEFSYPELL LCGEQRKKLI HTEDPFNDEH QERQEVEMLA 190 200 210 220 230 240 KKFEMKYGGK PRKHRKDRLQ DLIDIGFGYD ETDPFIDNSE AYDELVPASL TTKYGGFYIN 250 260 270 280 290 300 TGTLQFRQAS DTEEDDITDN QKHKPPKVPK IKEDDIEMKK RKRKEEGEKE KKPRKKVPKQ 310 320 330 340 350 360 LGVVALNSHK SEKKKKRYKD SLSLAAMIRK FQKEKDALKK ESNPKVPVTL STPSLNKPPC 370 380 390 400 410 420 AAAALGNDVP DLNLSSGDPD LPIFVSTNEH ELFQEAENAL EMLDDFDFDR LLDAASDGSP 430 440 450 460 470 480 LSESGGENGT TTQPTYTSQV MPKVVPTLPE GLPVLLEKRI EDLRVAAKLF DEEGRKKFFT 490 500 510 520 530 540 QDMNNILLDI ELQLQELGPV IRSGVYSHLE AFVPCNKETL VKRLKKLHLN VQDDRLREPL 550 560 570 580 590 600 QKLKLAVSNV MPEQLFKYQE DCQARSQAKC AKLQTDEERE KNGSEEDDDE KPGKRVIGPR 610 620 630 640 650 660 KKFHWDDTIR TLLCNLVEIK LGCYELEPNK SQSAEDYLKS FMETEVKPLW PKGWMQARML 670 680 690 700 710 720 FKESRSVHNH LTSAPAKKKV IPAPKPKVKE VMVKTLPLHS FPTMLKECSP KKDQKTPTSL 730 740 750 760 770 780 VASVSGPPTS SSTAAIAAAS SSSAPAQETI CLDDSLDEDL SFHSPSLDLV SEALAVINNG 790 800 810 820 830 840 NKGPPVGSRI SMPTTKPRPG LREEKLASIM SKLPLATPKK LDSTQTTHSS SLIAGHTGPV 850 860 870 880 890 900 PKKPQDLAHT GISSGLIAGS SIQNPKVSLE PLPARLLQQG LQRSSQIHTS SSSQTHVSSS 910 920 930 940 950 960 SQAQIAASSH ALGTSEAQDA SSLTQVTKVH QHSAVQQNYV SPLQATISKS QTNPVVKLSN 970 980 990 1000 1010 1020 NPQLSCSSSL IKTSDKPLMY RLPLSTPSPG NGSQGSHPLV SRTVPSTTTS SNYLAKAMVS 1030 1040 1050 1060 1070 1080 QISTQGFKSP FSMAASPKLA ASPKPATSPK PLPSPKPSAS PKPSLSAKPS VSTKLISKSN 1090 1100 1110 1120 1130 1140 PTPKPTVSPS SSSPNALVAQ GSHSSTNSPV HKQPSGMNIS RQSPTLNLLP SSRTSGLPPT 1150 1160 1170 1180 1190 1200 KNLQAPSKLT NSSSTGTVGK NSLSGIAMNV PASRGSNLNS SGANRTSLSG GTGSGTQGAT 1210 1220 1230 1240 1250 1260 KPLSTPHRPS TASGSSVVTA SVQSTAGASL LANASPLTLM TSPLSVTNQN VTPFGMLGGL 1270 1280 1290 1300 1310 1320 VPVTMPFQFP LEIFGFGTDT AGVTTTSGST SAAFHHSLTQ NLLKGLQPGG AQHAATLSHS 1330 1340 PLPAHLQQAF HDGGQSKGDT KLPRKSQ
	Isoform 2. Checksum: 20E88FEA63650CE2	FASTA	970	108,070
	10 20 30 40 50 60 MAEPRRVAFI SLSPVRRREA EYPGPEREPE YPREPPRLEP QPYREPARAE PPAPREPAPR 70 80 90 100 110 120 SDAQPPSREK PLPQREVSRA EPPMSLQREP PRPEPPPPFP PLPLQPPPPR ESASRAEQPP 130 140 150 160 170 180 RPPRETVRLE LVLKDPTDES CVEFSYPELL LCGEQRKKLI HTEDPFNDEH QERQEVEMLA 190 200 210 220 230 240 KKFEMKYGGK PRKHRKDRLQ DLIDIGFGYD ETDPFIDNSE AYDELVPASL TTKYGGFYIN 250 260 270 280 290 300 TGTLQFRQAS DTEEDDITDN QKHKPPKVPK IKEDDIEMKK RKRKEEGEKE KKPRKKVPKQ 310 320 330 340 350 360 LGVVALNSHK SEKKKKRYKD SLSLAAMIRK FQKEKDALKK ESNPKVPVTL STPSLNKPPC 370 380 390 400 410 420 AAAALGNDVP DLNLSSGDPD LPIFVSTNEH ELFQEAENAL EMLDDFDFDR LLDAASDGSP 430 440 450 460 470 480 LSESGGENGT TTQPTYTSQV MPKVVPTLPE GLPVLLEKRI EDLRVAAKLF DEEGRKKFFT 490 500 510 520 530 540 QDMNNILLDI ELQLQELGPV IRSGVYSHLE AFVPCNKETL VKRLKKLHLN VQDDRLREPL 550 560 570 580 590 600 QKLKLAVSNV MPEQLFKYQE DCQARSQAKC AKLQTDEERE KNGSEEDDDE KPGKRVIGPR 610 620 630 640 650 660 KKFHWDDTIR TLLCNLVEIK LGCYELEPNK SQSAEDYLKS FMETEVKPLW PKGWMQARML 670 680 690 700 710 720 FKESRSVHNH LTSAPAKKKV IPAPKPKVKE VMVKTLPLHS FPTMLKECSP KKDQKTPTSL 730 740 750 760 770 780 VASVSGPPTS SSTAAIAAAS SSSAPAQETI CLDDSLDEDL SFHSPSLDLV SEALAVINNG 790 800 810 820 830 840 NKGPPVGSRI SMPTTKPRPG LREEKLASIM SKLPLATPKK LDSTQTTHSS SLIAGHTGPV 850 860 870 880 890 900 PKKPQDLAHT GISSGLIAGS SIQNPKVSLE PLPARLLQQG LQRSSQIHTS SSSQTHVSSS 910 920 930 940 950 960 SQAQIAASSH ALGTSEAQDA SSLTQVTKVH QHSAVQQNYV SPLQATISKS QTNPVVKGPC 970 LRDVSEQFGP

References

[1]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-1347 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 478-1347 (ISOFORM 2). Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-1347 (ISOFORM 1). Tissue: Eye and Uterus.
[5]	"The nucleotide sequence of a long cDNA clone isolated from human." Nagase T., Kikuno R., Ohara O. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 325-1347 (ISOFORM 1). Tissue: Brain.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-584, MASS SPECTROMETRY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND THR-432, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, MASS SPECTROMETRY.
[9]	"Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells." Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams P.D. Mol. Cell. Biol. 29:758-770(2009) [PubMed: 19029251] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009220 Genomic DNA. No translation available. CH236950 Genomic DNA. Translation: EAL24038.1. Sequence problems. AK098644 mRNA. Translation: BAC05362.1. Different initiation. AK125956 mRNA. Translation: BAC86361.1. BC065034 mRNA. Translation: AAH65034.1. BC108249 mRNA. Translation: AAI08250.1. AB107352 mRNA. Translation: BAC67657.1.
IPI	IPI00785015. IPI00854561.
RefSeq	NP_775840.2.
UniGene	Hs.153458 Hs.624688
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q6ZU65.
Genome annotation databases
Ensembl	ENSG00000157741. Homo sapiens. [Contig view]
GeneID	254048.
KEGG	hsa:254048.
Organism-specific databases
GeneCards	GC07P138568.
HGNC	HGNC:21931. UBN2.
HPA	HPA019743.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6ZU65.
HOVERGEN	Q6ZU65.
Gene expression databases
ArrayExpress	Q6ZU65.
Bgee	Q6ZU65.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	92237.

Entry information

Entry name

UBN2_HUMAN

Accession

Primary (citable) accession number: Q6ZU65
Secondary accession number(s): A4D1S2 , Q2YDY4, Q6P1K0, Q86XN9, Q8N7D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	May 5, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)