Q6ZTQ3 (RASF6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras association domain-containing protein 6

Gene names

Name:

RASSF6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the induction of apoptosis, through both caspase-dependent and caspase-independent pathways. May act as a Ras effector protein. May suppress the serum-induced basal levels of NF-kappa-B By similarity. Ref.5
Subunit structure	Interacts with MOAP1 By similarity. Interaction with activated KRAS is still a matter of debate: interaction has been shown in the mouse (Ref.6), but not in human (Ref.5). Lack of interaction with MRAS, NRAS nor RRAS2 has also been reported (Ref.5). Ref.5
Tissue specificity	Highest expression in thymus, kidney and placenta. Also detected in colon, small intestine and lung. Tends to be down-regulated in 30-60% of tumors derived from breast, colon, kidney liver, rectum, pancreas, stomach and the thyroid gland compared to the normal counterpart. Ref.6
Sequence similarities	Contains 1 Ras-associating domain. Contains 1 SARAH domain.
Sequence caution	The sequence AAH58835.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAH18138.1 differs from that shown. Reason: Erroneous termination at position 148. Translated as Gln.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of apoptotic process Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STK4	Q13043	2	EBI-2933412,EBI-367376

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ZTQ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZTQ3-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Isoform 3 (identifier: Q6ZTQ3-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     23-80: SSDHLKKEEKMTMMAHQYPSWIFINEKTFITREQLNSLLKTYNIFYENQKNLHILYGE → HFLSSGLILVTRQL

Isoform 4 (identifier: Q6ZTQ3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     222-255: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Ras association domain-containing protein 6		PRO_0000240404
Regions
	Domain	218 – 306	89	Ras-associating
	Domain	313 – 360	48	SARAH
Natural variations
	Alternative sequence	1 – 32	32	Missing in isoform 2 and isoform 4.		VSP_019370
	Alternative sequence	23 – 80	58	SSDHL…ILYGE → HFLSSGLILVTRQL in isoform 3.		VSP_019371
	Alternative sequence	222 – 255	34	Missing in isoform 4.		VSP_019372
	Natural variant	163	1	S → P. Ref.4 Corresponds to variant rs12507775 [ dbSNP | Ensembl ].		VAR_026725
	Natural variant	306	1	A → G. Corresponds to variant rs17804499 [ dbSNP | Ensembl ].		VAR_034440

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 19AF7D4B1ABA5ED6	FASTA	369	43,384
	10 20 30 40 50 60 MLWEETGAAP APARASDLPY RISSDHLKKE EKMTMMAHQY PSWIFINEKT FITREQLNSL 70 80 90 100 110 120 LKTYNIFYEN QKNLHILYGE TEDGKLIVEG MLDIFWGVKR PIQLKIQDEK PFSSFTSMKS 130 140 150 160 170 180 SDVFSSKGMT RWGEFDDLYR ISELDRTQIP MSEKRNSQED YLSYHSNTLK PHAKDEPDSP 190 200 210 220 230 240 VLYRTMSEAA LVRKRMKPLM MDRKERQKNR ASINGHFYNH ETSIFIPAFE SETKVRVNSN 250 260 270 280 290 300 MRTEEVIKQL LQKFKIENSP QDFALHIIFA TGEQRRLKKT DIPLLQRLLQ GPSEKNARIF 310 320 330 340 350 360 LMDKDAEEIS SDVAQYINFH FSLLESILQR LNEEEKREIQ RIVTKFNKEK AIILKCLQNK LVIKTETTV
	Isoform 2 (A) [UniParc]. Checksum: 68D026FC687DFDD8	FASTA	337	39,804
	10 20 30 40 50 60 MTMMAHQYPS WIFINEKTFI TREQLNSLLK TYNIFYENQK NLHILYGETE DGKLIVEGML 70 80 90 100 110 120 DIFWGVKRPI QLKIQDEKPF SSFTSMKSSD VFSSKGMTRW GEFDDLYRIS ELDRTQIPMS 130 140 150 160 170 180 EKRNSQEDYL SYHSNTLKPH AKDEPDSPVL YRTMSEAALV RKRMKPLMMD RKERQKNRAS 190 200 210 220 230 240 INGHFYNHET SIFIPAFESE TKVRVNSNMR TEEVIKQLLQ KFKIENSPQD FALHIIFATG 250 260 270 280 290 300 EQRRLKKTDI PLLQRLLQGP SEKNARIFLM DKDAEEISSD VAQYINFHFS LLESILQRLN 310 320 330 EEEKREIQRI VTKFNKEKAI ILKCLQNKLV IKTETTV
	Isoform 3 (B) [UniParc]. Checksum: 91C71FBC2E18D6D9	FASTA	325	37,872
	10 20 30 40 50 60 MLWEETGAAP APARASDLPY RIHFLSSGLI LVTRQLTEDG KLIVEGMLDI FWGVKRPIQL 70 80 90 100 110 120 KIQDEKPFSS FTSMKSSDVF SSKGMTRWGE FDDLYRISEL DRTQIPMSEK RNSQEDYLSY 130 140 150 160 170 180 HSNTLKPHAK DEPDSPVLYR TMSEAALVRK RMKPLMMDRK ERQKNRASIN GHFYNHETSI 190 200 210 220 230 240 FIPAFESETK VRVNSNMRTE EVIKQLLQKF KIENSPQDFA LHIIFATGEQ RRLKKTDIPL 250 260 270 280 290 300 LQRLLQGPSE KNARIFLMDK DAEEISSDVA QYINFHFSLL ESILQRLNEE EKREIQRIVT 310 320 KFNKEKAIIL KCLQNKLVIK TETTV
	Isoform 4 [UniParc]. Checksum: 7B4510499A52918E	FASTA	303	35,809
	10 20 30 40 50 60 MTMMAHQYPS WIFINEKTFI TREQLNSLLK TYNIFYENQK NLHILYGETE DGKLIVEGML 70 80 90 100 110 120 DIFWGVKRPI QLKIQDEKPF SSFTSMKSSD VFSSKGMTRW GEFDDLYRIS ELDRTQIPMS 130 140 150 160 170 180 EKRNSQEDYL SYHSNTLKPH AKDEPDSPVL YRTMSEAALV RKRMKPLMMD RKERQKNRAS 190 200 210 220 230 240 INGHFYNHEI ENSPQDFALH IIFATGEQRR LKKTDIPLLQ RLLQGPSEKN ARIFLMDKDA 250 260 270 280 290 300 EEISSDVAQY INFHFSLLES ILQRLNEEEK REIQRIVTKF NKEKAIILKC LQNKLVIKTE TTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RASSF6, a member of the RASSF family of putative tumor suppressors, is transcriptionally repressed in lung and breast cancers." Burbee D.G., White M.A., Miller D.S., Minna J.D., Muller C.Y. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Small intestine.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANT PRO-163. Tissue: Prostate.
[5]	"Ras-association domain family protein 6 induces apoptosis via both caspase-dependent and caspase-independent pathways." Ikeda M., Hirabayashi S., Fujiwara N., Mori H., Kawata A., Iida J., Bao Y., Sato Y., Iida T., Sugimura H., Hata Y. Exp. Cell Res. 313:1484-1495(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, LACK OF INTERACTION WITH KRAS; MRAS; NRAS AND RRAS2.
[6]	"RASSF6 is a novel member of the RASSF family of tumor suppressors." Allen N.P., Donninger H., Vos M.D., Eckfeld K., Hesson L., Gordon L., Birrer M.J., Latif F., Clark G.J. Oncogene 26:6203-6211(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY217665 mRNA. Translation: AAO61690.1. AY217664 mRNA. Translation: AAO61689.1. AK126346 mRNA. Translation: BAC86530.1. CR749283 mRNA. Translation: CAH18138.1. Sequence problems. BC058835 mRNA. Translation: AAH58835.1. Different initiation.
IPI	IPI00176707. IPI00384451. IPI00398796. IPI00760776.
RefSeq	NP_001257320.1. NM_001270391.1. NP_001257321.1. NM_001270392.1. NP_803876.1. NM_177532.4. NP_958834.1. NM_201431.2.
UniGene	Hs.529677.
3D structure databases
ProteinModelPortal	Q6ZTQ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZTQ3. 2 interactions.
STRING	9606.ENSP00000340578.
PTM databases
PhosphoSite	Q6ZTQ3.
Polymorphism databases
DMDM	74749658.
Proteomic databases
PaxDb	Q6ZTQ3.
PRIDE	Q6ZTQ3.
Protocols and materials databases
DNASU	166824.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307439; ENSP00000303877; ENSG00000169435. ENST00000335049; ENSP00000335582; ENSG00000169435. ENST00000342081; ENSP00000340578; ENSG00000169435. ENST00000395777; ENSP00000379123; ENSG00000169435.
GeneID	166824.
KEGG	hsa:166824.
UCSC	uc003hhc.1. human. uc010iik.1. human. uc010iil.1. human.
Organism-specific databases
CTD	166824.
GeneCards	GC04M074437.
HGNC	HGNC:20796. RASSF6.
HPA	HPA037711.
MIM	612620. gene.
neXtProt	NX_Q6ZTQ3.
PharmGKB	PA134900075.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG244901.
HOGENOM	HOG000231738.
HOVERGEN	HBG054302.
InParanoid	Q6ZTQ3.
KO	K09854.
OMA	RGMTRWG.
OrthoDB	EOG49W2FK.
PhylomeDB	Q6ZTQ3.
Gene expression databases
Bgee	Q6ZTQ3.
CleanEx	HS_RASSF6.
Genevestigator	Q6ZTQ3.
GermOnline	ENSG00000169435. Homo sapiens.
Family and domain databases
InterPro	IPR000159. Ras-assoc. IPR011524. SARAH_dom. [Graphical view]
Pfam	PF00788. RA. 1 hit. [Graphical view]
SMART	SM00314. RA. 1 hit. [Graphical view]
PROSITE	PS50200. RA. 1 hit. PS50951. SARAH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	166824.
NextBio	88631.
SOURCE	Search...

Entry information

Entry name

RASF6_HUMAN

Accession

Primary (citable) accession number: Q6ZTQ3
Secondary accession number(s): Q68DT2 , Q6PDA6, Q86WG9, Q86WH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.