Q6ZSI9 (CAN12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-12
EC=3.4.22.-

Alternative name(s):
Calcium-activated neutral proteinase 12
Short name=CANP 12

Gene names

Name:

CAPN12

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease By similarity.
Catalytic activity	Broad endopeptidase specificity.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 1 EF-hand domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Calpain-12		PRO_0000207730
Regions
	Domain	45 – 341	297	Calpain catalytic
	Domain	620 – 655	36	EF-hand
	Calcium binding	633 – 644	12	Potential
	Region	342 – 540	199	Domain III
	Region	541 – 719	179	Domain IV
Sites
	Active site	105	1	By similarity
	Active site	259	1	By similarity
	Active site	283	1	By similarity
Natural variations
	Natural variant	287	1	C → R. Corresponds to variant rs12983010 [ dbSNP | Ensembl ].		VAR_051516

Sequences

	Sequence		Length	Mass (Da)
	Q6ZSI9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BEACE2E5DD5839F2	FASTA	719	81,037
	10 20 30 40 50 60 MASSSGRVTI QLVDEEAGVG AGRLQLFRGQ SYEAIRAACL DSGILFRDPY FPAGPDALGY 70 80 90 100 110 120 DQLGPDSEKA KGVKWMRPHE FCAEPKFICE DMSRTDVCQG SLGNCWFLAA AASLTLYPRL 130 140 150 160 170 180 LRRVVPPGQD FQHGYAGVFH FQLWQFGRWM DVVVDDRLPV REGKLMFVRS EQRNEFWAPL 190 200 210 220 230 240 LEKAYAKLHG SYEVMRGGHM NEAFVDFTGG VGEVLYLRQN SMGLFSALRH ALAKESLVGA 250 260 270 280 290 300 TALSDRGEYR TEEGLVKGHA YSITGTHKVF LGFTKVRLLR LRNPWGCVEW TGAWSDSCPR 310 320 330 340 350 360 WDTLPTECRD ALLVKKEDGE FWMELRDFLL HFDTVQICSL SPEVLGPSPE GGGWHVHTFQ 370 380 390 400 410 420 GRWVRGFNSG GSQPNAETFW TNPQFRLTLL EPDEEDDEDE EGPWGGWGAA GARGPARGGR 430 440 450 460 470 480 TPKCTVLLSL IQRNRRRLRA KGLTYLTVGF HVFQIPEELL GLWDSPRSHA LLPRLLRADR 490 500 510 520 530 540 SPLSARRDVT RRCCLRPGHY LVVPSTAHAG DEADFTLRVF SERRHTAVEI DDVISADLQS 550 560 570 580 590 600 LQGPYLPLEL GLEQLFQELA GEEEELNASQ LQALLSIALE PARAHTSTPR EIGLRTCEQL 610 620 630 640 650 660 LQCFGHGQSL ALHHFQQLWG YLLEWQAIFN KFDEDTSGTM NSYELRLALN AAGFHLNNQL 670 680 690 700 710 TQTLTSRYRD SRLRVDFERF VSCVAHLTCI FCHCSQHLDG GEGVICLTHR QWMEVATFS

References

[1]

"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK127398 mRNA. Translation: BAC86959.1.
IPI	IPI00418873.
RefSeq	NP_653292.2. NM_144691.3.
UniGene	Hs.731775.
3D structure databases
ProteinModelPortal	Q6ZSI9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000331636.
Protein family/group databases
MEROPS	C02.017.
PTM databases
PhosphoSite	Q6ZSI9.
Polymorphism databases
DMDM	54035703.
Proteomic databases
PaxDb	Q6ZSI9.
PRIDE	Q6ZSI9.
Protocols and materials databases
DNASU	147968.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328867; ENSP00000331636; ENSG00000182472.
GeneID	147968.
KEGG	hsa:147968.
UCSC	uc002ojd.1. human.
Organism-specific databases
CTD	147968.
GeneCards	GC19M039220.
HGNC	HGNC:13249. CAPN12.
HPA	HPA043492.
MIM	608839. gene.
neXtProt	NX_Q6ZSI9.
PharmGKB	PA134863447.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323867.
HOGENOM	HOG000232035.
HOVERGEN	HBG012645.
InParanoid	Q6ZSI9.
KO	K04740.
OMA	FSERRHT.
OrthoDB	EOG4P5K8M.
PhylomeDB	Q6ZSI9.
Gene expression databases
Bgee	Q6ZSI9.
CleanEx	HS_CAPN12.
Genevestigator	Q6ZSI9.
GermOnline	ENSG00000182472. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. [Graphical view]
SUPFAM	SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	147968.
NextBio	85797.
SOURCE	Search...

Entry information

Entry name

CAN12_HUMAN

Accession

Primary (citable) accession number: Q6ZSI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.