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Protein

Rho family-interacting cell polarization regulator 1

Gene

RIPOR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector protein for Rho-type small GTPases that plays a role in cell polarity and directional migration (PubMed:27807006). Acts as an adapter protein, linking active Rho proteins to STK24 and STK26 kinases, and hence positively regulates Golgi reorientation in polarized cell migration upon Rho activation (PubMed:27807006). Involved in the subcellular relocation of STK26 from the Golgi to cytoplasm punctae in a Rho- and PDCD10-dependent manner upon serum stimulation (PubMed:27807006).1 Publication

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB

GO - Biological processi

  • cellular response to chemokine Source: UniProtKB
  • cellular response to starvation Source: UniProtKB
  • establishment of Golgi localization Source: UniProtKB
  • negative regulation of Rho guanyl-nucleotide exchange factor activity Source: UniProtKB
  • negative regulation of Rho protein signal transduction Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of intracellular protein transport Source: UniProtKB
  • protein localization to Golgi apparatus Source: UniProtKB
  • response to wounding Source: UniProtKB
  • Rho protein signal transduction Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Rho family-interacting cell polarization regulator 1
Gene namesi
Name:RIPOR1
Synonyms:FAM65A, KIAA1930
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000039523.17.
HGNCiHGNC:25836. RIPOR1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000039523.
PharmGKBiPA142671875.

Polymorphism and mutation databases

BioMutaiFAM65A.
DMDMi74711310.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002891101 – 1223Rho family-interacting cell polarization regulator 1Add BLAST1223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineCombined sources1
Modified residuei349PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei355PhosphothreonineCombined sources1
Modified residuei456PhosphoserineBy similarity1
Modified residuei459PhosphoserineBy similarity1
Modified residuei874PhosphoserineBy similarity1
Modified residuei875PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZS17.
MaxQBiQ6ZS17.
PaxDbiQ6ZS17.
PeptideAtlasiQ6ZS17.
PRIDEiQ6ZS17.

PTM databases

iPTMnetiQ6ZS17.
PhosphoSitePlusiQ6ZS17.

Expressioni

Gene expression databases

BgeeiENSG00000039523.
CleanExiHS_FAM65A.
ExpressionAtlasiQ6ZS17. baseline and differential.
GenevisibleiQ6ZS17. HS.

Organism-specific databases

HPAiHPA005923.

Interactioni

Subunit structurei

Interacts (via N-terminus) with RHOA (GTP-bound form); this interaction links active RHOA to STK24 and STK26 kinases (PubMed:27807006). Interacts with RHOB (PubMed:27807006). Interacts with RHOC (PubMed:27807006). Interacts (via C-terminus) with PDCD10; this interaction occurs in a Rho-independent manner (PubMed:27807006). Interacts (via C-terminus) with STK24; this interaction occurs in a PDCD10-dependent and Rho-independent manner (PubMed:27807006). Interacts (via C-terminus) with STK26; this interaction occurs in a PDCD10-dependent and Rho-independent manner (PubMed:27807006). Interacts (via N-terminus) with 14-3-3 proteins; these interactions occur in a Rho-dependent manner (PubMed:27807006).1 Publication

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122715. 5 interactors.
IntActiQ6ZS17. 4 interactors.
STRINGi9606.ENSP00000400099.

Structurei

3D structure databases

ProteinModelPortaliQ6ZS17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili89 – 114Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi481 – 767Pro-richAdd BLAST287

Sequence similaritiesi

Belongs to the RIPOR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGQR. Eukaryota.
ENOG410ZI73. LUCA.
GeneTreeiENSGT00490000043360.
HOGENOMiHOG000112476.
HOVERGENiHBG053834.
InParanoidiQ6ZS17.
OMAiGQFPELQ.
OrthoDBiEOG091G018M.
PhylomeDBiQ6ZS17.
TreeFamiTF329332.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR031780. FAM65_N.
IPR026136. RIPOR3.
PANTHERiPTHR15829. PTHR15829. 1 hit.
PfamiView protein in Pfam
PF15903. PL48. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZS17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSLSVRPQR RLLSARVNRS QSFAGVLGSH ERGPSLSFRS FPVFSPPGPP
60 70 80 90 100
RKPPALSRVS RMFSVAHPAA KVPQPERLDL VYTALKRGLT AYLEVHQQEQ
110 120 130 140 150
EKLQGQIRES KRNSRLGFLY DLDKQVKSIE RFLRRLEFHA SKIDELYEAY
160 170 180 190 200
CVQRRLRDGA YNMVRAYTTG SPGSREARDS LAEATRGHRE YTESMCLLES
210 220 230 240 250
ELEAQLGEFH LRMKGLAGFA RLCVGDQYEI CMKYGRQRWK LRGRIEGSGK
260 270 280 290 300
QVWDSEETIF LPLLTEFLSI KVTELKGLAN HVVVGSVSCE TKDLFAALPQ
310 320 330 340 350
VVAVDINDLG TIKLSLEVTW SPFDKDDQPS AASSVNKAST VTKRFSTYSQ
360 370 380 390 400
SPPDTPSLRE QAFYNMLRRQ EELENGTAWS LSSESSDDSS SPQLSGTARH
410 420 430 440 450
SPAPRPLVQQ PEPLPIQVAF RRPETPSSGP LDEEGAVAPV LANGHAPYSR
460 470 480 490 500
TLSHISEASV DAALAEASVE AVGPESLAWG PSPPTHPAPT HGEHPSPVPP
510 520 530 540 550
ALDPGHSATS STLGTTGSVP TSTDPAPSAH LDSVHKSTDS GPSELPGPTH
560 570 580 590 600
TTTGSTYSAI TTTHSAPSPL THTTTGSTHK PIISTLTTTG PTLNIIGPVQ
610 620 630 640 650
TTTSPTHTMP SPTHTTASPT HTSTSPTHTP TSPTHKTSMS PPTTTSPTPS
660 670 680 690 700
GMGLVQTATS PTHPTTSPTH PTTSPILINV SPSTSLELAT LSSPSKHSDP
710 720 730 740 750
TLPGTDSLPC SPPVSNSYTQ ADPMAPRTPH PSPAHSSRKP LTSPAPDPSE
760 770 780 790 800
STVQSLSPTP SPPTPAPQHS DLCLAMAVQT PVPTAAGGSG DRSLEEALGA
810 820 830 840 850
LMAALDDYRG QFPELQGLEQ EVTRLESLLM QRQGLTRSRA SSLSITVEHA
860 870 880 890 900
LESFSFLNED EDEDNDVPGD RPPSSPEAGA EDSIDSPSAR PLSTGCPALD
910 920 930 940 950
AALVRHLYHC SRLLLKLGTF GPLRCQEAWA LERLLREARV LEAVCEFSRR
960 970 980 990 1000
WEIPASSAQE VVQFSASRPG FLTFWDQCTE RLSCFLCPVE RVLLTFCNQY
1010 1020 1030 1040 1050
GARLSLRQPG LAEAVCVKFL EDALGQKLPR RPQPGPGEQL TVFQFWSFVE
1060 1070 1080 1090 1100
TLDSPTMEAY VTETAEEVLL VRNLNSDDQA VVLKALRLAP EGRLRRDGLR
1110 1120 1130 1140 1150
ALSSLLVHGN NKVMAAVSTQ LRSLSLGPTF RERALLCFLD QLEDEDVQTR
1160 1170 1180 1190 1200
VAGCLALGCI KAPEGIEPLV YLCQTDTEAV REAARQSLQQ CGEEGQSAHR
1210 1220
RLEESLDALP RIFGPGSMAS TAF
Length:1,223
Mass (Da):132,308
Last modified:July 5, 2004 - v1
Checksum:i4C6C9A387E93DEC7
GO
Isoform 2 (identifier: Q6ZS17-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-38: Missing.

Show »
Length:1,219
Mass (Da):131,873
Checksum:i200460D45BD48AF9
GO
Isoform 3 (identifier: Q6ZS17-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNTKKRGSPARTHSM
     35-38: Missing.

Note: No experimental confirmation available.
Show »
Length:1,233
Mass (Da):133,426
Checksum:i478DD57FEC6CB7F6
GO
Isoform 4 (identifier: Q6ZS17-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTIWQMQKQAQRGSPARTHSM
     35-38: Missing.

Note: No experimental confirmation available.
Show »
Length:1,239
Mass (Da):134,198
Checksum:iB250037B6F22D2C4
GO

Sequence cautioni

Q6ZS17: The sequence BAB14678 differs from that shown. Reason: Erroneous termination at position 256. Translated as Glu.Curated
Q6ZS17: The sequence BAB67823 differs from that shown. Reason: Frameshift at position 579.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152V → A in BAG57170 (PubMed:14702039).Curated1
Sequence conflicti653G → S in BAB14678 (PubMed:14702039).Curated1
Sequence conflicti766A → T in BAG57170 (PubMed:14702039).Curated1
Sequence conflicti831Missing in BAB67823 (PubMed:11572484).Curated1
Sequence conflicti1012A → T in BAB14678 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0433151M → MNTKKRGSPARTHSM in isoform 3. 1 Publication1
Alternative sequenceiVSP_0450021M → MTIWQMQKQAQRGSPARTHS M in isoform 4. 1 Publication1
Alternative sequenceiVSP_02590335 – 38Missing in isoform 2, isoform 3 and isoform 4. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023787 mRNA. Translation: BAB14678.1. Sequence problems.
AK293748 mRNA. Translation: BAG57170.1.
AK295677 mRNA. Translation: BAG58534.1.
AC027682 Genomic DNA. No translation available.
AK127792 mRNA. Translation: BAC87139.1.
AB067517 mRNA. Translation: BAB67823.1. Frameshift.
BC001850 mRNA. Translation: AAH01850.3.
BC054512 mRNA. Translation: AAH54512.1.
BC098587 mRNA. Translation: AAH98587.1.
AL834312 mRNA. Translation: CAD38982.1.
CCDSiCCDS10840.1. [Q6ZS17-2]
CCDS54026.1. [Q6ZS17-4]
CCDS54027.1. [Q6ZS17-3]
CCDS54028.1. [Q6ZS17-1]
RefSeqiNP_001180451.1. NM_001193522.1. [Q6ZS17-1]
NP_001180452.1. NM_001193523.1. [Q6ZS17-4]
NP_001180453.1. NM_001193524.1. [Q6ZS17-3]
NP_078795.2. NM_024519.3. [Q6ZS17-2]
UniGeneiHs.152717.

Genome annotation databases

EnsembliENST00000042381; ENSP00000042381; ENSG00000039523. [Q6ZS17-2]
ENST00000379312; ENSP00000368614; ENSG00000039523. [Q6ZS17-1]
ENST00000422602; ENSP00000400099; ENSG00000039523. [Q6ZS17-4]
ENST00000428437; ENSP00000389456; ENSG00000039523. [Q6ZS17-3]
GeneIDi79567.
KEGGihsa:79567.
UCSCiuc002eth.4. human. [Q6ZS17-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRIPR1_HUMAN
AccessioniPrimary (citable) accession number: Q6ZS17
Secondary accession number(s): B4DEQ9
, B4DIM2, E9PBS3, Q4G0A4, Q7Z5R7, Q8NDA4, Q96J39, Q96PV8, Q9H8D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: October 25, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families