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Q6ZQK5

- ACAP2_MOUSE

UniProt

Q6ZQK5 - ACAP2_MOUSE

Protein

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Gene

Acap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).By similarity

    Enzyme regulationi

    GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri414 – 43724C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. protein binding Source: UniProtKB
    3. Rab GTPase binding Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to nerve growth factor stimulus Source: UniProtKB
    2. protein localization to endosome Source: UniProtKB
    3. regulation of ARF GTPase activity Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
    Alternative name(s):
    Centaurin-beta-2
    Short name:
    Cnt-b2
    Gene namesi
    Name:Acap2
    Synonyms:Centb2, Kiaa0041Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1925868. Acap2.

    Subcellular locationi

    Endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 770770Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2PRO_0000306385Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei384 – 3841Phosphoserine1 Publication
    Modified residuei521 – 5211PhosphoserineBy similarity
    Modified residuei734 – 7341Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6ZQK5.
    PaxDbiQ6ZQK5.
    PRIDEiQ6ZQK5.

    PTM databases

    PhosphoSiteiQ6ZQK5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6ZQK5.
    BgeeiQ6ZQK5.
    GenevestigatoriQ6ZQK5.

    Interactioni

    Subunit structurei

    Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits ACAP2 to membranes. Interacts with MICALL1; the interaction is indirect through RAB35.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6ZQK5.
    SMRiQ6ZQK5. Positions 7-360, 378-750.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 226226BARSequence AnalysisAdd
    BLAST
    Domaini266 – 36196PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini399 – 520122Arf-GAPPROSITE-ProRule annotationAdd
    BLAST
    Repeati632 – 66130ANK 1Sequence AnalysisAdd
    BLAST
    Repeati665 – 69430ANK 2Sequence AnalysisAdd
    BLAST
    Repeati698 – 72730ANK 3Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 3 ANK repeats.PROSITE-ProRule annotation
    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
    Contains 1 BAR domain.Sequence Analysis
    Contains 1 PH domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri414 – 43724C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    GeneTreeiENSGT00750000117227.
    HOGENOMiHOG000220815.
    HOVERGENiHBG050889.
    InParanoidiQ6ZQK5.
    KOiK12489.
    OMAiQHATDED.
    OrthoDBiEOG7PVWNT.
    PhylomeDBiQ6ZQK5.
    TreeFamiTF318315.

    Family and domain databases

    Gene3Di1.20.1270.60. 1 hit.
    1.25.40.20. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR027267. AH/BAR-dom.
    IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00248. ANK. 3 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q6ZQK5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKMTVDFEEC LKDSPRFRAA LEEVEGDVAE LELKLDKLVK LCIAMIDTGK    50
    AFCVANKQFM NGIRDLAQYS SNDAVVETSL TKFSDSLQEM INFHTILFDQ 100
    TQRSIKAQLQ NFVKEDLRKF KDAKKQFEKV SEEKENALVK NAQVQRNKQH 150
    EVEEAANILT ATRKCFRHIA LDYVLQINVL QSKRRSEILK SMLSFMYAHL 200
    AFFHQGYDLF SELGPYMKDL GAQLDRLVVD AAKEKREMEQ KHSTIQQKDF 250
    SSDDSKLEYN VDAANGIVME GYLFKRASNA FKTWNRRWFS IQNNQLVYQK 300
    KFKDSPTVVV EDLRLCTVKH CEDIERRFCF EVVSPTKSCM LQADSEKLRQ 350
    AWIKAVQTSI ATAYREKGDE SEKLDKKSSP STGSLDSGNE SKEKLLKGES 400
    ALQRVQCIPG NTSCCDCGLA DPRWASINLG ITLCIECSGI HRSLGVHFSK 450
    VRSLTLDTWE PELLKLMCEL GNDVINRVYE AKLEKMGVKK PQPGQRQEKE 500
    AYIRAKYVER KFVDKYSALL SPSEQEKRII SKSCEDQRLS HARASVHTPV 550
    KSNDSGIQQC SEDGRESLPS TVSANSLYEP EGERQESSVF LDSKHLNPGL 600
    QLYRASYEKN LPKMAEALAH GADVNWANSD ENQATPLIQA VLGGSLVTCE 650
    FLLQNGANVN QRDVQGRGPL HHATVLGHTG QVCLFLKRGA NQHATDEEGK 700
    DPLSIAVEAA NADIVTLLRL ARMNEEMRES EGLYGQPGDE TYQDIFRDFS 750
    QMASNNPEKL NRFQQDSQKF 770
    Length:770
    Mass (Da):87,211
    Last modified:October 2, 2007 - v2
    Checksum:i8882864BB7D248F2
    GO
    Isoform 21 Publication (identifier: Q6ZQK5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         78-95: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:752
    Mass (Da):85,129
    Checksum:i0AB83EF8D1035EB7
    GO

    Sequence cautioni

    The sequence BAC97851.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti526 – 5261E → K in AAH46455. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei78 – 9518Missing in isoform 2. 1 PublicationVSP_052554Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129041 mRNA. Translation: BAC97851.1. Different initiation.
    AK020591 mRNA. Translation: BAB32141.3.
    AK147319 mRNA. Translation: BAE27843.1.
    BC046455 mRNA. Translation: AAH46455.1.
    CCDSiCCDS49823.1. [Q6ZQK5-1]
    RefSeqiNP_084414.1. NM_030138.2. [Q6ZQK5-1]
    UniGeneiMm.274646.

    Genome annotation databases

    EnsembliENSMUST00000058033; ENSMUSP00000061501; ENSMUSG00000049076. [Q6ZQK5-1]
    GeneIDi78618.
    KEGGimmu:78618.
    UCSCiuc007yxb.1. mouse. [Q6ZQK5-1]
    uc007yxc.1. mouse. [Q6ZQK5-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129041 mRNA. Translation: BAC97851.1 . Different initiation.
    AK020591 mRNA. Translation: BAB32141.3 .
    AK147319 mRNA. Translation: BAE27843.1 .
    BC046455 mRNA. Translation: AAH46455.1 .
    CCDSi CCDS49823.1. [Q6ZQK5-1 ]
    RefSeqi NP_084414.1. NM_030138.2. [Q6ZQK5-1 ]
    UniGenei Mm.274646.

    3D structure databases

    ProteinModelPortali Q6ZQK5.
    SMRi Q6ZQK5. Positions 7-360, 378-750.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6ZQK5.

    Proteomic databases

    MaxQBi Q6ZQK5.
    PaxDbi Q6ZQK5.
    PRIDEi Q6ZQK5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000058033 ; ENSMUSP00000061501 ; ENSMUSG00000049076 . [Q6ZQK5-1 ]
    GeneIDi 78618.
    KEGGi mmu:78618.
    UCSCi uc007yxb.1. mouse. [Q6ZQK5-1 ]
    uc007yxc.1. mouse. [Q6ZQK5-2 ]

    Organism-specific databases

    CTDi 23527.
    MGIi MGI:1925868. Acap2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5347.
    GeneTreei ENSGT00750000117227.
    HOGENOMi HOG000220815.
    HOVERGENi HBG050889.
    InParanoidi Q6ZQK5.
    KOi K12489.
    OMAi QHATDED.
    OrthoDBi EOG7PVWNT.
    PhylomeDBi Q6ZQK5.
    TreeFami TF318315.

    Miscellaneous databases

    NextBioi 349208.
    PROi Q6ZQK5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6ZQK5.
    Bgeei Q6ZQK5.
    Genevestigatori Q6ZQK5.

    Family and domain databases

    Gene3Di 1.20.1270.60. 1 hit.
    1.25.40.20. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR027267. AH/BAR-dom.
    IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00248. ANK. 3 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: BrainImported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6JImported.
      Tissue: Urinary bladderImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-770.
      Strain: Czech IIImported.
      Tissue: Mammary glandImported.
    4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-734, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
      Kobayashi H., Fukuda M.
      J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB35 AND MICALL1.

    Entry informationi

    Entry nameiACAP2_MOUSE
    AccessioniPrimary (citable) accession number: Q6ZQK5
    Secondary accession number(s): Q3UHL4, Q811F3, Q9CTS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3