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Q6ZQK5

- ACAP2_MOUSE

UniProt

Q6ZQK5 - ACAP2_MOUSE

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Protein

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Gene

Acap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).By similarity

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri414 – 43724C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: InterPro
  2. Rab GTPase binding Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to nerve growth factor stimulus Source: UniProtKB
  2. protein localization to endosome Source: UniProtKB
  3. regulation of ARF GTPase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Centaurin-beta-2
Short name:
Cnt-b2
Gene namesi
Name:Acap2
Synonyms:Centb2, Kiaa0041Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1925868. Acap2.

Subcellular locationi

Endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2PRO_0000306385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei384 – 3841Phosphoserine1 Publication
Modified residuei521 – 5211PhosphoserineBy similarity
Modified residuei734 – 7341Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6ZQK5.
PaxDbiQ6ZQK5.
PRIDEiQ6ZQK5.

PTM databases

PhosphoSiteiQ6ZQK5.

Expressioni

Gene expression databases

BgeeiQ6ZQK5.
ExpressionAtlasiQ6ZQK5. baseline and differential.
GenevestigatoriQ6ZQK5.

Interactioni

Subunit structurei

Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits ACAP2 to membranes. Interacts with MICALL1; the interaction is indirect through RAB35.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6ZQK5.
SMRiQ6ZQK5. Positions 7-360, 378-750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 226226BARSequence AnalysisAdd
BLAST
Domaini266 – 36196PHPROSITE-ProRule annotationAdd
BLAST
Domaini399 – 520122Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Repeati632 – 66130ANK 1Sequence AnalysisAdd
BLAST
Repeati665 – 69430ANK 2Sequence AnalysisAdd
BLAST
Repeati698 – 72730ANK 3Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 BAR domain.Sequence Analysis
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri414 – 43724C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000220815.
HOVERGENiHBG050889.
InParanoidiQ6ZQK5.
KOiK12489.
OMAiQHATDED.
OrthoDBiEOG7PVWNT.
PhylomeDBiQ6ZQK5.
TreeFamiTF318315.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q6ZQK5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMTVDFEEC LKDSPRFRAA LEEVEGDVAE LELKLDKLVK LCIAMIDTGK
60 70 80 90 100
AFCVANKQFM NGIRDLAQYS SNDAVVETSL TKFSDSLQEM INFHTILFDQ
110 120 130 140 150
TQRSIKAQLQ NFVKEDLRKF KDAKKQFEKV SEEKENALVK NAQVQRNKQH
160 170 180 190 200
EVEEAANILT ATRKCFRHIA LDYVLQINVL QSKRRSEILK SMLSFMYAHL
210 220 230 240 250
AFFHQGYDLF SELGPYMKDL GAQLDRLVVD AAKEKREMEQ KHSTIQQKDF
260 270 280 290 300
SSDDSKLEYN VDAANGIVME GYLFKRASNA FKTWNRRWFS IQNNQLVYQK
310 320 330 340 350
KFKDSPTVVV EDLRLCTVKH CEDIERRFCF EVVSPTKSCM LQADSEKLRQ
360 370 380 390 400
AWIKAVQTSI ATAYREKGDE SEKLDKKSSP STGSLDSGNE SKEKLLKGES
410 420 430 440 450
ALQRVQCIPG NTSCCDCGLA DPRWASINLG ITLCIECSGI HRSLGVHFSK
460 470 480 490 500
VRSLTLDTWE PELLKLMCEL GNDVINRVYE AKLEKMGVKK PQPGQRQEKE
510 520 530 540 550
AYIRAKYVER KFVDKYSALL SPSEQEKRII SKSCEDQRLS HARASVHTPV
560 570 580 590 600
KSNDSGIQQC SEDGRESLPS TVSANSLYEP EGERQESSVF LDSKHLNPGL
610 620 630 640 650
QLYRASYEKN LPKMAEALAH GADVNWANSD ENQATPLIQA VLGGSLVTCE
660 670 680 690 700
FLLQNGANVN QRDVQGRGPL HHATVLGHTG QVCLFLKRGA NQHATDEEGK
710 720 730 740 750
DPLSIAVEAA NADIVTLLRL ARMNEEMRES EGLYGQPGDE TYQDIFRDFS
760 770
QMASNNPEKL NRFQQDSQKF
Length:770
Mass (Da):87,211
Last modified:October 2, 2007 - v2
Checksum:i8882864BB7D248F2
GO
Isoform 21 Publication (identifier: Q6ZQK5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     78-95: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:752
Mass (Da):85,129
Checksum:i0AB83EF8D1035EB7
GO

Sequence cautioni

The sequence BAC97851.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti526 – 5261E → K in AAH46455. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei78 – 9518Missing in isoform 2. 1 PublicationVSP_052554Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129041 mRNA. Translation: BAC97851.1. Different initiation.
AK020591 mRNA. Translation: BAB32141.3.
AK147319 mRNA. Translation: BAE27843.1.
BC046455 mRNA. Translation: AAH46455.1.
CCDSiCCDS49823.1. [Q6ZQK5-1]
RefSeqiNP_084414.1. NM_030138.2. [Q6ZQK5-1]
UniGeneiMm.274646.

Genome annotation databases

EnsembliENSMUST00000058033; ENSMUSP00000061501; ENSMUSG00000049076. [Q6ZQK5-1]
GeneIDi78618.
KEGGimmu:78618.
UCSCiuc007yxb.1. mouse. [Q6ZQK5-1]
uc007yxc.1. mouse. [Q6ZQK5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129041 mRNA. Translation: BAC97851.1 . Different initiation.
AK020591 mRNA. Translation: BAB32141.3 .
AK147319 mRNA. Translation: BAE27843.1 .
BC046455 mRNA. Translation: AAH46455.1 .
CCDSi CCDS49823.1. [Q6ZQK5-1 ]
RefSeqi NP_084414.1. NM_030138.2. [Q6ZQK5-1 ]
UniGenei Mm.274646.

3D structure databases

ProteinModelPortali Q6ZQK5.
SMRi Q6ZQK5. Positions 7-360, 378-750.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6ZQK5.

Proteomic databases

MaxQBi Q6ZQK5.
PaxDbi Q6ZQK5.
PRIDEi Q6ZQK5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000058033 ; ENSMUSP00000061501 ; ENSMUSG00000049076 . [Q6ZQK5-1 ]
GeneIDi 78618.
KEGGi mmu:78618.
UCSCi uc007yxb.1. mouse. [Q6ZQK5-1 ]
uc007yxc.1. mouse. [Q6ZQK5-2 ]

Organism-specific databases

CTDi 23527.
MGIi MGI:1925868. Acap2.
Rougei Search...

Phylogenomic databases

eggNOGi COG5347.
GeneTreei ENSGT00760000118874.
HOGENOMi HOG000220815.
HOVERGENi HBG050889.
InParanoidi Q6ZQK5.
KOi K12489.
OMAi QHATDED.
OrthoDBi EOG7PVWNT.
PhylomeDBi Q6ZQK5.
TreeFami TF318315.

Miscellaneous databases

NextBioi 349208.
PROi Q6ZQK5.
SOURCEi Search...

Gene expression databases

Bgeei Q6ZQK5.
ExpressionAtlasi Q6ZQK5. baseline and differential.
Genevestigatori Q6ZQK5.

Family and domain databases

Gene3Di 1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: BrainImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6JImported.
    Tissue: Urinary bladderImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-770.
    Strain: Czech IIImported.
    Tissue: Mammary glandImported.
  4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-734, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
    Kobayashi H., Fukuda M.
    J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB35 AND MICALL1.

Entry informationi

Entry nameiACAP2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQK5
Secondary accession number(s): Q3UHL4, Q811F3, Q9CTS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3