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Protein

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Gene

Acap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).By similarity

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri414 – 437C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,5-bisphosphate binding Source: MGI
  • Rab GTPase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Centaurin-beta-2
Short name:
Cnt-b2
Gene namesi
Name:Acap2
Synonyms:Centb2, Kiaa0041Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1925868. Acap2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003063851 – 770Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei521PhosphoserineBy similarity1
Modified residuei573PhosphoserineCombined sources1
Modified residuei576PhosphoserineCombined sources1
Modified residuei734PhosphotyrosineCombined sources1
Modified residuei767PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZQK5.
PaxDbiQ6ZQK5.
PeptideAtlasiQ6ZQK5.
PRIDEiQ6ZQK5.

PTM databases

iPTMnetiQ6ZQK5.
PhosphoSitePlusiQ6ZQK5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049076.
GenevisibleiQ6ZQK5. MM.

Interactioni

Subunit structurei

Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits ACAP2 to membranes. Interacts with MICALL1; the interaction is indirect through RAB35.1 Publication

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061501.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQK5.
SMRiQ6ZQK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 226BARSequence analysisAdd BLAST226
Domaini266 – 361PHPROSITE-ProRule annotationAdd BLAST96
Domaini399 – 520Arf-GAPPROSITE-ProRule annotationAdd BLAST122
Repeati632 – 661ANK 1Sequence analysisAdd BLAST30
Repeati665 – 694ANK 2Sequence analysisAdd BLAST30
Repeati698 – 727ANK 3Sequence analysisAdd BLAST30

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri414 – 437C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000220815.
HOVERGENiHBG050889.
InParanoidiQ6ZQK5.
KOiK12489.
OMAiRFVCGMP.
OrthoDBiEOG091G029F.
PhylomeDBiQ6ZQK5.
TreeFamiTF318315.

Family and domain databases

CDDicd00204. ANK. 1 hit.
Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR027267. AH/BAR_dom_sf.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR001164. ArfGAP.
IPR037278. ARFGAP/RecO.
IPR011993. PH-like_dom_sf.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PRINTSiPR00405. REVINTRACTNG.
SMARTiView protein in SMART
SM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SUPFAMiSSF103657. SSF103657. 1 hit.
SSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF57863. SSF57863. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q6ZQK5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMTVDFEEC LKDSPRFRAA LEEVEGDVAE LELKLDKLVK LCIAMIDTGK
60 70 80 90 100
AFCVANKQFM NGIRDLAQYS SNDAVVETSL TKFSDSLQEM INFHTILFDQ
110 120 130 140 150
TQRSIKAQLQ NFVKEDLRKF KDAKKQFEKV SEEKENALVK NAQVQRNKQH
160 170 180 190 200
EVEEAANILT ATRKCFRHIA LDYVLQINVL QSKRRSEILK SMLSFMYAHL
210 220 230 240 250
AFFHQGYDLF SELGPYMKDL GAQLDRLVVD AAKEKREMEQ KHSTIQQKDF
260 270 280 290 300
SSDDSKLEYN VDAANGIVME GYLFKRASNA FKTWNRRWFS IQNNQLVYQK
310 320 330 340 350
KFKDSPTVVV EDLRLCTVKH CEDIERRFCF EVVSPTKSCM LQADSEKLRQ
360 370 380 390 400
AWIKAVQTSI ATAYREKGDE SEKLDKKSSP STGSLDSGNE SKEKLLKGES
410 420 430 440 450
ALQRVQCIPG NTSCCDCGLA DPRWASINLG ITLCIECSGI HRSLGVHFSK
460 470 480 490 500
VRSLTLDTWE PELLKLMCEL GNDVINRVYE AKLEKMGVKK PQPGQRQEKE
510 520 530 540 550
AYIRAKYVER KFVDKYSALL SPSEQEKRII SKSCEDQRLS HARASVHTPV
560 570 580 590 600
KSNDSGIQQC SEDGRESLPS TVSANSLYEP EGERQESSVF LDSKHLNPGL
610 620 630 640 650
QLYRASYEKN LPKMAEALAH GADVNWANSD ENQATPLIQA VLGGSLVTCE
660 670 680 690 700
FLLQNGANVN QRDVQGRGPL HHATVLGHTG QVCLFLKRGA NQHATDEEGK
710 720 730 740 750
DPLSIAVEAA NADIVTLLRL ARMNEEMRES EGLYGQPGDE TYQDIFRDFS
760 770
QMASNNPEKL NRFQQDSQKF
Length:770
Mass (Da):87,211
Last modified:October 2, 2007 - v2
Checksum:i8882864BB7D248F2
GO
Isoform 21 Publication (identifier: Q6ZQK5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     78-95: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:752
Mass (Da):85,129
Checksum:i0AB83EF8D1035EB7
GO

Sequence cautioni

The sequence BAC97851 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526E → K in AAH46455 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05255478 – 95Missing in isoform 2. 1 PublicationAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129041 mRNA. Translation: BAC97851.1. Different initiation.
AK020591 mRNA. Translation: BAB32141.3.
AK147319 mRNA. Translation: BAE27843.1.
BC046455 mRNA. Translation: AAH46455.1.
CCDSiCCDS49823.1. [Q6ZQK5-1]
RefSeqiNP_084414.1. NM_030138.2. [Q6ZQK5-1]
UniGeneiMm.274646.

Genome annotation databases

EnsembliENSMUST00000058033; ENSMUSP00000061501; ENSMUSG00000049076. [Q6ZQK5-1]
GeneIDi78618.
KEGGimmu:78618.
UCSCiuc007yxb.1. mouse. [Q6ZQK5-1]
uc007yxc.1. mouse. [Q6ZQK5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACAP2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQK5
Secondary accession number(s): Q3UHL4, Q811F3, Q9CTS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 22, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot