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Protein

E3 ubiquitin-protein ligase Jade-2

Gene

Jade2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A. Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (PubMed:25018020).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI

GO - Biological processi

  • histone H3 acetylation Source: UniProtKB
  • histone H4-K12 acetylation Source: UniProtKB
  • histone H4-K5 acetylation Source: UniProtKB
  • histone H4-K8 acetylation Source: UniProtKB
  • neuron projection extension Source: MGI
  • positive regulation of neurogenesis Source: MGI
  • protein autoubiquitination Source: MGI
  • protein polyubiquitination Source: MGI
  • regulation of neurogenesis Source: MGI
  • SMAD protein signal transduction Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214847 HATs acetylate histones
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Jade-21 Publication (EC:2.3.2.271 Publication)
Alternative name(s):
Jade family PHD finger protein 2
PHD finger protein 15
Gene namesi
Name:Jade2
Synonyms:Kiaa0239, Phf15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1924151 Jade2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Strongly decreased level of KDM1A polyubiquitination resulting in increased level of KDM1A protein. Decelerated emergence of neural progenitors and mature neurons. Embryonic stem cells grow in aggregates with smoother-edged, rounder-shaped cell clones and fail to organize in rosettes with surrounding cells exhibiting neuronal morphology with extensive arborization. Decreased expression of neural markers.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002535331 – 829E3 ubiquitin-protein ligase Jade-2Add BLAST829

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei32N6-acetyllysineCombined sources1
Modified residuei38N6-acetyllysineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei298N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZQF7
PaxDbiQ6ZQF7
PRIDEiQ6ZQF7

PTM databases

iPTMnetiQ6ZQF7
PhosphoSitePlusiQ6ZQF7

Expressioni

Gene expression databases

BgeeiENSMUSG00000020387
CleanExiMM_PHF15
ExpressionAtlasiQ6ZQF7 baseline and differential
GenevisibleiQ6ZQF7 MM

Interactioni

Subunit structurei

Component of the HBO1 complex composed at least of ING4 or ING5, MYST2/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3 (By similarity). Interacts (via C-terminus) with KDM1A (via AOD/Tower domain) (PubMed:25018020).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020655

Structurei

3D structure databases

ProteinModelPortaliQ6ZQF7
SMRiQ6ZQF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The first PHD domain is essential for its E3 ubiquitin ligase activity.By similarity

Sequence similaritiesi

Belongs to the JADE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0954 Eukaryota
COG5141 LUCA
GeneTreeiENSGT00740000114866
HOGENOMiHOG000220882
HOVERGENiHBG053585
InParanoidiQ6ZQF7
KOiK22155
OMAiMAMSEWP
OrthoDBiEOG091G0T5C
PhylomeDBiQ6ZQF7
TreeFamiTF316118

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR019542 Enhancer_polycomb-like_N
IPR034732 EPHD
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF10513 EPL1, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51805 EPHD, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZQF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKRRKYSI SSDNSDTTDG HVTSTSASRC SKLPSSTKSG WPRQNEKKPS
60 70 80 90 100
EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE
110 120 130 140 150
PVVRLLPPLK GPPTQMSPDS PTLGEGAHPD WPGGSRYDLD EIDAYWLELL
160 170 180 190 200
NSELKEMEKP ELDELTLERV LEELETLCHQ NMAQAIETQE GLGIEYDEDV
210 220 230 240 250
VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV
260 270 280 290 300
QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS
310 320 330 340 350
HIPASRWALS CSLCKECTGT CIQCSMPSCI TAFHVTCAFD RGLEMRTILA
360 370 380 390 400
DNDEVKFKSL CQEHSDGGPR SEPTSEPVEP SQAVEDLEKV TLRKQRLQQL
410 420 430 440 450
EENFYELVEP AEVAERLDLA EALVDFIYQY WKLKRRANAN QPLLTPKTDE
460 470 480 490 500
VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHTICKLQ
510 520 530 540 550
EQIFHLQMKL IEQDLCREPS GRRSKGKKND SKRKGREGPK GSSPEKKEKV
560 570 580 590 600
KAGPESVLGQ LGLSTSFPID GTFFNSWLAQ SVQITAEDMA MSEWSLNSGH
610 620 630 640 650
REDPAPGLLS EELLQDEETL LSFMRDPSLR PGDPARKARG RTRLPAKKKP
660 670 680 690 700
SPLQDGPSAR TTPDKQPKKA WAQDGKGTQG PPMRKPPRRT SSHLPSSPAA
710 720 730 740 750
GDCPVPATLE SPPPLASEIL DKTAPMASDL NVQVPGPTVS PKPLGRLRPP
760 770 780 790 800
REMKVSRKSP GARSDAGTGL PSAVAERPKV SLHFDTEADG YFSDEEMSDS
810 820
EVEAEDSGVQ RASREAGAEE VVRMGVLAS
Length:829
Mass (Da):92,174
Last modified:October 17, 2006 - v2
Checksum:i0B5B6275F7BD6769
GO
Isoform 2 (identifier: Q6ZQF7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-103: V → VSWAARGGQAKASLFITTILFP
     518-561: Missing.

Show »
Length:806
Mass (Da):89,677
Checksum:i1BCB06096D69FAFC
GO

Sequence cautioni

The sequence BAC97907 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021052103V → VSWAARGGQAKASLFITTIL FP in isoform 2. Curated1
Alternative sequenceiVSP_021053518 – 561Missing in isoform 2. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129097 mRNA Translation: BAC97907.1 Different initiation.
AK147453 mRNA Translation: BAE27922.1
BC117856 mRNA Translation: AAI17857.1
BC117857 mRNA Translation: AAI17858.1
BN000285 mRNA Translation: CAE30498.1
CCDSiCCDS24662.1 [Q6ZQF7-1]
RefSeqiNP_955003.2, NM_199299.3 [Q6ZQF7-1]
XP_006534532.1, XM_006534469.3 [Q6ZQF7-1]
XP_006534533.1, XM_006534470.3 [Q6ZQF7-1]
XP_011247611.1, XM_011249309.2 [Q6ZQF7-1]
XP_017170321.1, XM_017314832.1 [Q6ZQF7-1]
UniGeneiMm.259996

Genome annotation databases

EnsembliENSMUST00000020655; ENSMUSP00000020655; ENSMUSG00000020387 [Q6ZQF7-1]
ENSMUST00000109091; ENSMUSP00000104719; ENSMUSG00000020387 [Q6ZQF7-1]
GeneIDi76901
KEGGimmu:76901
UCSCiuc007iuq.1 mouse [Q6ZQF7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiJADE2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQF7
Secondary accession number(s): Q3UHD5, Q6IE83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: March 28, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health