Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase Jade-2

Gene

Jade2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A. Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (PubMed:25018020).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI

GO - Biological processi

  • histone H3 acetylation Source: UniProtKB
  • histone H4-K12 acetylation Source: UniProtKB
  • histone H4-K5 acetylation Source: UniProtKB
  • histone H4-K8 acetylation Source: UniProtKB
  • neuron projection extension Source: MGI
  • positive regulation of neurogenesis Source: MGI
  • protein autoubiquitination Source: MGI
  • protein polyubiquitination Source: MGI
  • regulation of neurogenesis Source: MGI
  • SMAD protein signal transduction Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Jade-21 Publication (EC:2.3.2.271 Publication)
Alternative name(s):
Jade family PHD finger protein 2
PHD finger protein 15
Gene namesi
Name:Jade2
Synonyms:Kiaa0239, Phf15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1924151. Jade2.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Strongly decreased level of KDM1A polyubiquitination resulting in increased level of KDM1A protein. Decelerated emergence of neural progenitors and mature neurons. Embryonic stem cells grow in aggregates with smoother-edged, rounder-shaped cell clones and fail to organize in rosettes with surrounding cells exhibiting neuronal morphology with extensive arborization. Decreased expression of neural markers.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002535331 – 829E3 ubiquitin-protein ligase Jade-2Add BLAST829

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei32N6-acetyllysineCombined sources1
Modified residuei38N6-acetyllysineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei298N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZQF7.
PaxDbiQ6ZQF7.
PRIDEiQ6ZQF7.

PTM databases

iPTMnetiQ6ZQF7.
PhosphoSitePlusiQ6ZQF7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020387.
CleanExiMM_PHF15.
ExpressionAtlasiQ6ZQF7. baseline and differential.
GenevisibleiQ6ZQF7. MM.

Interactioni

Subunit structurei

Component of the HBO1 complex composed at least of ING4 or ING5, MYST2/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3 (By similarity). Interacts (via C-terminus) with KDM1A (via AOD/Tower domain) (PubMed:25018020).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020655.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQF7.
SMRiQ6ZQF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The first PHD domain is essential for its E3 ubiquitin ligase activity.By similarity

Sequence similaritiesi

Belongs to the JADE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0954. Eukaryota.
COG5141. LUCA.
GeneTreeiENSGT00740000114866.
HOGENOMiHOG000220882.
HOVERGENiHBG053585.
InParanoidiQ6ZQF7.
OMAiRPPRESK.
OrthoDBiEOG091G0T5C.
PhylomeDBiQ6ZQF7.
TreeFamiTF316118.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR019542. Enhancer_polycomb-like_N.
IPR034732. EPHD.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF10513. EPL1. 1 hit.
SMARTiView protein in SMART
SM00249. PHD. 2 hits.
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiView protein in PROSITE
PS51805. EPHD. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZQF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKRRKYSI SSDNSDTTDG HVTSTSASRC SKLPSSTKSG WPRQNEKKPS
60 70 80 90 100
EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE
110 120 130 140 150
PVVRLLPPLK GPPTQMSPDS PTLGEGAHPD WPGGSRYDLD EIDAYWLELL
160 170 180 190 200
NSELKEMEKP ELDELTLERV LEELETLCHQ NMAQAIETQE GLGIEYDEDV
210 220 230 240 250
VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV
260 270 280 290 300
QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS
310 320 330 340 350
HIPASRWALS CSLCKECTGT CIQCSMPSCI TAFHVTCAFD RGLEMRTILA
360 370 380 390 400
DNDEVKFKSL CQEHSDGGPR SEPTSEPVEP SQAVEDLEKV TLRKQRLQQL
410 420 430 440 450
EENFYELVEP AEVAERLDLA EALVDFIYQY WKLKRRANAN QPLLTPKTDE
460 470 480 490 500
VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHTICKLQ
510 520 530 540 550
EQIFHLQMKL IEQDLCREPS GRRSKGKKND SKRKGREGPK GSSPEKKEKV
560 570 580 590 600
KAGPESVLGQ LGLSTSFPID GTFFNSWLAQ SVQITAEDMA MSEWSLNSGH
610 620 630 640 650
REDPAPGLLS EELLQDEETL LSFMRDPSLR PGDPARKARG RTRLPAKKKP
660 670 680 690 700
SPLQDGPSAR TTPDKQPKKA WAQDGKGTQG PPMRKPPRRT SSHLPSSPAA
710 720 730 740 750
GDCPVPATLE SPPPLASEIL DKTAPMASDL NVQVPGPTVS PKPLGRLRPP
760 770 780 790 800
REMKVSRKSP GARSDAGTGL PSAVAERPKV SLHFDTEADG YFSDEEMSDS
810 820
EVEAEDSGVQ RASREAGAEE VVRMGVLAS
Length:829
Mass (Da):92,174
Last modified:October 17, 2006 - v2
Checksum:i0B5B6275F7BD6769
GO
Isoform 2 (identifier: Q6ZQF7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-103: V → VSWAARGGQAKASLFITTILFP
     518-561: Missing.

Show »
Length:806
Mass (Da):89,677
Checksum:i1BCB06096D69FAFC
GO

Sequence cautioni

The sequence BAC97907 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021052103V → VSWAARGGQAKASLFITTIL FP in isoform 2. Curated1
Alternative sequenceiVSP_021053518 – 561Missing in isoform 2. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129097 mRNA. Translation: BAC97907.1. Different initiation.
AK147453 mRNA. Translation: BAE27922.1.
BC117856 mRNA. Translation: AAI17857.1.
BC117857 mRNA. Translation: AAI17858.1.
BN000285 mRNA. Translation: CAE30498.1.
CCDSiCCDS24662.1. [Q6ZQF7-1]
RefSeqiNP_955003.2. NM_199299.3. [Q6ZQF7-1]
XP_006534532.1. XM_006534469.3. [Q6ZQF7-1]
XP_006534533.1. XM_006534470.3. [Q6ZQF7-1]
XP_011247611.1. XM_011249309.2. [Q6ZQF7-1]
XP_017170321.1. XM_017314832.1. [Q6ZQF7-1]
UniGeneiMm.259996.

Genome annotation databases

EnsembliENSMUST00000020655; ENSMUSP00000020655; ENSMUSG00000020387. [Q6ZQF7-1]
ENSMUST00000109091; ENSMUSP00000104719; ENSMUSG00000020387. [Q6ZQF7-1]
GeneIDi76901.
KEGGimmu:76901.
UCSCiuc007iuq.1. mouse. [Q6ZQF7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiJADE2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQF7
Secondary accession number(s): Q3UHD5, Q6IE83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: July 5, 2017
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families