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Protein

DNA topoisomerase 2-binding protein 1

Gene

Topbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication (By similarity). Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:14718568). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). Induces a large increase in the kinase activity of ATR (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-binding protein 1
Alternative name(s):
DNA topoisomerase II-beta-binding protein 1
Short name:
TopBP1
DNA topoisomerase II-binding protein 1
Gene namesi
Name:Topbp1
Synonyms:Kiaa0259
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920018. Topbp1.

Subcellular locationi

  • Nucleus
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
  • Cytoplasmcytoskeletonspindle pole
  • Chromosome

  • Note: Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX-independent manner (By similarity). Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • centrosome Source: MGI
  • chromosome Source: UniProtKB
  • condensed nuclear chromosome Source: MGI
  • cytoplasm Source: MGI
  • Golgi apparatus Source: MGI
  • male germ cell nucleus Source: MGI
  • nucleus Source: MGI
  • PML body Source: MGI
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15151515DNA topoisomerase 2-binding protein 1PRO_0000072632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei298 – 2981PhosphothreonineBy similarity
Modified residuei782 – 7821PhosphothreonineBy similarity
Modified residuei851 – 8511PhosphothreonineBy similarity
Modified residuei862 – 8621PhosphoserineCombined sources
Modified residuei863 – 8631PhosphoserineCombined sources
Modified residuei866 – 8661PhosphoserineBy similarity
Modified residuei888 – 8881PhosphoserineBy similarity
Modified residuei890 – 8901PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to X-ray irradiation.By similarity
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6ZQF0.
MaxQBiQ6ZQF0.
PaxDbiQ6ZQF0.
PeptideAtlasiQ6ZQF0.
PRIDEiQ6ZQF0.

PTM databases

iPTMnetiQ6ZQF0.
PhosphoSiteiQ6ZQF0.

Expressioni

Tissue specificityi

Highly expressed in testis.1 Publication

Developmental stagei

Levels increase during the first 3 weeks after birth and remain high in the fourth week.1 Publication

Gene expression databases

BgeeiENSMUSG00000032555.
CleanExiMM_TOPBP1.
ExpressionAtlasiQ6ZQF0. baseline and differential.
GenevisibleiQ6ZQF0. MM.

Interactioni

Subunit structurei

Interacts with POLE. Interacts with RAD9A. Interacts with UBR5. Interacts with E2F1. Interacts with PML. Interacts with SMARCA2. Interacts with SMARCA4. Interacts with RHNO1. May interact with TOP2B. Interacts with TICRR. Interacts with HELB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231681. 8 interactions.
IntActiQ6ZQF0. 10 interactions.
STRINGi10090.ENSMUSP00000035164.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQF0.
SMRiQ6ZQF0. Positions 7-285, 331-444, 553-747, 905-996, 1262-1485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 18989BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini195 – 28490BRCT 2PROSITE-ProRule annotationAdd
BLAST
Domaini353 – 44391BRCT 3PROSITE-ProRule annotationAdd
BLAST
Domaini551 – 63686BRCT 4PROSITE-ProRule annotationAdd
BLAST
Domaini644 – 74198BRCT 5PROSITE-ProRule annotationAdd
BLAST
Domaini902 – 99392BRCT 6PROSITE-ProRule annotationAdd
BLAST
Domaini1255 – 134793BRCT 7PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1510 – 15134Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1207 – 124640Pro-richAdd
BLAST

Sequence similaritiesi

Contains 7 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1929. Eukaryota.
ENOG410XPFH. LUCA.
GeneTreeiENSGT00840000129846.
HOVERGENiHBG067053.
InParanoidiQ6ZQF0.
KOiK10728.
OMAiCAQEYKH.
OrthoDBiEOG091G02MY.
PhylomeDBiQ6ZQF0.
TreeFamiTF326403.

Family and domain databases

Gene3Di3.40.50.10190. 6 hits.
InterProiIPR001357. BRCT_dom.
IPR026993. Topbp1.
[Graphical view]
PANTHERiPTHR13561:SF32. PTHR13561:SF32. 3 hits.
PfamiPF00533. BRCT. 4 hits.
PF12738. PTCB-BRCT. 2 hits.
[Graphical view]
SMARTiSM00292. BRCT. 8 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 7 hits.
PROSITEiPS50172. BRCT. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ZQF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRNDQEPFL VKFLKSSDNS ECFFKALESI KELQSEDYLQ IITDEEALKI
60 70 80 90 100
RENDKSLYIC DRFSGTVFDH LKQLGCRIVG PQVVTFCMRH QQCVPRAEHP
110 120 130 140 150
VYNMIMSDVT VSCTSLDKDK REEVHKYVQM MGGRVYRDLN VSVTHLIAGE
160 170 180 190 200
VGSKKYLVAA NLKKPILLPS WIKTLWEKSQ EKKITKYTDV NMEDFKCPIF
210 220 230 240 250
LGCIICVTGL NGIHRKTVQQ LTAKHGGQYM GQLKMNECTH LIVQEPKGQK
260 270 280 290 300
YECARRWNVH CVTLQWFHDS IEKGFCQDES IYKAETRVEA KMVPDTSTPT
310 320 330 340 350
AQSNAESHTL ADVSHISNIN GSCVNETMFG STTSKLECSL ENLENLDISM
360 370 380 390 400
FQAPEDLLDG CRIYLCGFSG RKLDKLRRLI NSGGGVRFNQ LNEDVTHVIV
410 420 430 440 450
GDYDDDVRQF WSKSSHRPHV VGAKWLLECF TKGYILPEES YIHTNYQPAG
460 470 480 490 500
IAVSDQPGNQ TAVLDKSGSF SKSALVPAER LQQADEDLLA QYGNDDSTMV
510 520 530 540 550
EAKLSEALEP EVGPCPGSAH REPCDDSTHI SVQEENKSSV SHCILDDSTV
560 570 580 590 600
REEGLFSQKS FLVLGFSVEN KCNIVDIIRE HAGKIVSLPS RIVADYAVVP
610 620 630 640 650
LLGCEVDVTV GEVVTNTWLV TCIDNQTLVD PKSNPLFTPV SVMSGVTPLE
660 670 680 690 700
DCVISFSQCV GAERDSLVFL ANHLGASVQE FFVRKANAKK GMLASTHLIV
710 720 730 740 750
KEPTGSKYEA AKKWSLPAVN ISWLLETARI GKRADENHFL VDNAPKQEQV
760 770 780 790 800
LETKIPNGVS SNPDLPAHPD AHLEIHRKKA VTPLDMNRFQ SRAFRAVISQ
810 820 830 840 850
QRGQDPTFPP VRQPLTKEPS LHLDTPSKFL SKDKLFKPSF DVTDALAALE
860 870 880 890 900
TPNAASQKRK LSSPLSEVIV RNLTVALANS SRNTDSHSAS PQLKGAHLEE
910 920 930 940 950
EETRKPLDSV VVCVSKKLSK KQSELNGVAA SLGAEYRWSF DETVTHFIYQ
960 970 980 990 1000
GRANDSNREY KSAKERGVHI VSEHWLLECA QEYKHLPESL YPHTYNPKMS
1010 1020 1030 1040 1050
LDINTVQDGR LCNSRAPLAV SASKDDGPDH LSVEGNETNT MGTNDKESPL
1060 1070 1080 1090 1100
LNGSGRDDCK GALTQALEMR ENFQKQLQEI MSATCIVKTP AQKTCMSRSS
1110 1120 1130 1140 1150
CNSASSTPDS ARSVRSGRSR VLEALRQSRQ AVPDVNTEPS QNEQIIWDDP
1160 1170 1180 1190 1200
TAREERARLA SNLQWPSDPT QHSELQVEIK MPDDSPSRKP VYHSEIAEQA
1210 1220 1230 1240 1250
SCVTQAPGHP GSEEPEPPVA ERPLIPEPQA PAVASPLAKP PVAPQPADKI
1260 1270 1280 1290 1300
ETQEETHRKV KKQYVFQMSS LNSQERIDYC RLIKDLGGSV IEKQCSDPSC
1310 1320 1330 1340 1350
THMVVGYPLR NEKYLASMAA GKWVLHRSYL DACKTAGRFV QEEDYEWGSS
1360 1370 1380 1390 1400
SILDALPDVT EHQQKLALAA MRWRKRIQQS QESGIVEGAF SGWKAILRVD
1410 1420 1430 1440 1450
RPREAGFKRL LQAGGAKVLS GHPEPLLKDA THLFCDFNKL KPDDCRVFIA
1460 1470 1480 1490 1500
EATAQNMVCL KTEYIADYLM LESPPCADNY RVSEAALFHN KKGGPGLPQK
1510
RKTPAENVVK RPRVH
Length:1,515
Mass (Da):168,860
Last modified:April 26, 2005 - v2
Checksum:iE2E729D2E57B6F2F
GO

Sequence cautioni

The sequence AAH07170 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC39345 differs from that shown. Reason: Frameshift at position 183. Curated
The sequence BAC97914 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241V → I in AAH07170 (PubMed:15489334).Curated
Sequence conflicti461 – 4611T → I in AAH07170 (PubMed:15489334).Curated
Sequence conflicti521 – 5211R → L in AAH07170 (PubMed:15489334).Curated
Sequence conflicti607 – 6071D → E in AAH07170 (PubMed:15489334).Curated
Sequence conflicti835 – 8351L → P in AAH62111 (PubMed:15489334).Curated
Sequence conflicti1041 – 10411M → I in AAH07170 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1420 – 14201S → P in strain: FVB/N. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129104 mRNA. Translation: BAC97914.1. Different initiation.
BC006707 mRNA. Translation: AAH06707.1.
BC007170 mRNA. Translation: AAH07170.1. Different initiation.
BC026608 mRNA. Translation: AAH26608.1.
BC049797 mRNA. Translation: AAH49797.1.
BC062111 mRNA. Translation: AAH62111.1.
AK084654 mRNA. Translation: BAC39241.2.
AK085031 mRNA. Translation: BAC39345.1. Frameshift.
CCDSiCCDS23453.1.
RefSeqiNP_795953.2. NM_176979.5.
XP_006511770.1. XM_006511707.2.
UniGeneiMm.259893.

Genome annotation databases

EnsembliENSMUST00000035164; ENSMUSP00000035164; ENSMUSG00000032555.
GeneIDi235559.
KEGGimmu:235559.
UCSCiuc009rgl.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129104 mRNA. Translation: BAC97914.1. Different initiation.
BC006707 mRNA. Translation: AAH06707.1.
BC007170 mRNA. Translation: AAH07170.1. Different initiation.
BC026608 mRNA. Translation: AAH26608.1.
BC049797 mRNA. Translation: AAH49797.1.
BC062111 mRNA. Translation: AAH62111.1.
AK084654 mRNA. Translation: BAC39241.2.
AK085031 mRNA. Translation: BAC39345.1. Frameshift.
CCDSiCCDS23453.1.
RefSeqiNP_795953.2. NM_176979.5.
XP_006511770.1. XM_006511707.2.
UniGeneiMm.259893.

3D structure databases

ProteinModelPortaliQ6ZQF0.
SMRiQ6ZQF0. Positions 7-285, 331-444, 553-747, 905-996, 1262-1485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231681. 8 interactions.
IntActiQ6ZQF0. 10 interactions.
STRINGi10090.ENSMUSP00000035164.

PTM databases

iPTMnetiQ6ZQF0.
PhosphoSiteiQ6ZQF0.

Proteomic databases

EPDiQ6ZQF0.
MaxQBiQ6ZQF0.
PaxDbiQ6ZQF0.
PeptideAtlasiQ6ZQF0.
PRIDEiQ6ZQF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035164; ENSMUSP00000035164; ENSMUSG00000032555.
GeneIDi235559.
KEGGimmu:235559.
UCSCiuc009rgl.1. mouse.

Organism-specific databases

CTDi11073.
MGIiMGI:1920018. Topbp1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1929. Eukaryota.
ENOG410XPFH. LUCA.
GeneTreeiENSGT00840000129846.
HOVERGENiHBG067053.
InParanoidiQ6ZQF0.
KOiK10728.
OMAiCAQEYKH.
OrthoDBiEOG091G02MY.
PhylomeDBiQ6ZQF0.
TreeFamiTF326403.

Enzyme and pathway databases

ReactomeiR-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ6ZQF0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032555.
CleanExiMM_TOPBP1.
ExpressionAtlasiQ6ZQF0. baseline and differential.
GenevisibleiQ6ZQF0. MM.

Family and domain databases

Gene3Di3.40.50.10190. 6 hits.
InterProiIPR001357. BRCT_dom.
IPR026993. Topbp1.
[Graphical view]
PANTHERiPTHR13561:SF32. PTHR13561:SF32. 3 hits.
PfamiPF00533. BRCT. 4 hits.
PF12738. PTCB-BRCT. 2 hits.
[Graphical view]
SMARTiSM00292. BRCT. 8 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 7 hits.
PROSITEiPS50172. BRCT. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOPB1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQF0
Secondary accession number(s): Q6P6P0
, Q80Y33, Q8BUI0, Q8BUK1, Q8R348, Q91VX3, Q922X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.