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Protein

Nuclear envelope integral membrane protein 1

Gene

Nemp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear envelope integral membrane protein 1
Gene namesi
Name:Nemp1
Synonyms:Kiaa0286, Tmem194, Tmem194a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2446113. Nemp1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei244 – 264HelicalSequence analysisAdd BLAST21
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi366S → A or E: Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-376; A/E-380; A/E-419 and A/E-420. 1 Publication1
Mutagenesisi376S → A or E: Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-380; A/E-419 and A/E-420. 1 Publication1
Mutagenesisi380S → A or E: Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-376; A/E-419 and A/E-420. 1 Publication1
Mutagenesisi419S → A or E: Partial loss of phosphorylation and loss of interaction with RAN; when associated with A/E-366; A/E-376; A/E-380 and A/E-420. 1 Publication1
Mutagenesisi420S → A or E: Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-376; A/E-380 and A/E-419. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 44Sequence analysisAdd BLAST44
ChainiPRO_000005074545 – 437Nuclear envelope integral membrane protein 1Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi123N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei366PhosphoserineBy similarity1
Modified residuei419PhosphoserineBy similarity1
Modified residuei420PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation may regulate its interaction with RAN-GTP.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ6ZQE4.
MaxQBiQ6ZQE4.
PaxDbiQ6ZQE4.
PRIDEiQ6ZQE4.

PTM databases

iPTMnetiQ6ZQE4.
PhosphoSitePlusiQ6ZQE4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040195.
CleanExiMM_TMEM194.
ExpressionAtlasiQ6ZQE4. baseline and differential.
GenevisibleiQ6ZQE4. MM.

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with RAN-GTP.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RanP628277EBI-12595939,EBI-286564

Protein-protein interaction databases

BioGridi229124. 1 interactor.
IntActiQ6ZQE4. 2 interactors.
STRINGi10090.ENSMUSP00000045988.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 295A; required for its colocalization with lamins at the nuclear envelope1 PublicationAdd BLAST112
Regioni334 – 437Required for nuclear localization1 PublicationAdd BLAST104
Regioni334 – 403B; required for interaction with RAN-GTP1 PublicationAdd BLAST70

Domaini

The transmembrane domains are required and sufficient for its oligomerization.By similarity

Sequence similaritiesi

Belongs to the NEMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3817. Eukaryota.
ENOG410ZZKM. LUCA.
GeneTreeiENSGT00390000002174.
HOGENOMiHOG000122573.
HOVERGENiHBG052180.
InParanoidiQ6ZQE4.
OMAiHDIWTRI.
OrthoDBiEOG091G09L2.
PhylomeDBiQ6ZQE4.
TreeFamiTF314831.

Family and domain databases

InterProiView protein in InterPro
IPR019358. NEMP_fam.
PfamiView protein in Pfam
PF10225. NEMP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ZQE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGIKVSVW SAVGPGPRCW GAGGGGGATW LLLVVAGCVV CGSADVNVVM
60 70 80 90 100
LQESQVDMNS SQQFCYKNVL IPKWHDIWTR IQVRVNSSKL VRVTQVDNEE
110 120 130 140 150
KLKELEQFSI WNFFSSFLKE KLNDTYVNVG LYSTKTCLKV EMIEKDTTYS
160 170 180 190 200
VTVTRRFDPK LFLVFLLGLT LFFCGDLLSR SQIFYYSTGM SVGIVASLLI
210 220 230 240 250
VIFMISKFMP KRSPIYVILV GGWSFSLYLI QLVFKNLQEI WRSYWHYLLS
260 270 280 290 300
YILTVGFMSF AVCYKYGPLE NERSINLLTW TLQLLGLGLM YSSIQIPHVA
310 320 330 340 350
FALIVIALCT KNLEYPIHWL CSTYRRMCKA SGKPVPPRLL TEEEYRIQGE
360 370 380 390 400
VETQKALQEL REFCNSPECS AWKTISRIQS PKRFADFVEG SFHLTPNEVS
410 420 430
VHEQEYGLGS IFTQDEELSS EEEGSEYPTF TQNNFLT
Length:437
Mass (Da):49,817
Last modified:November 23, 2004 - v2
Checksum:iF6B25929100CC1D9
GO

Sequence cautioni

Q6ZQE4: The sequence BAC97920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129110 mRNA. Translation: BAC97920.1. Different initiation.
CCDSiCCDS48720.1.
RefSeqiNP_001106682.1. NM_001113211.1.
UniGeneiMm.251075.

Genome annotation databases

EnsembliENSMUST00000048099; ENSMUSP00000045988; ENSMUSG00000040195.
GeneIDi210035.
KEGGimmu:210035.
UCSCiuc007hke.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNEMP1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families