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Q6ZQB6

- VIP2_MOUSE

UniProt

Q6ZQB6 - VIP2_MOUSE

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Protein
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2
Gene
Ppip5k2, Hisppd1, Kiaa0433, Vip2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.

Kineticsi

  1. KM=0.23 µM for InsP61 Publication
  2. KM=0.54 µM for InsP7

Vmax=1.70 nmol/min/mg enzyme with InsP6 as substrate

Vmax=5.23 nmol/min/mg enzyme with InsP7 as substrate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401ATP By similarity
Binding sitei193 – 1931ATP By similarity
Binding sitei200 – 2001ATP By similarity
Binding sitei219 – 2191ATP By similarity
Binding sitei254 – 2541Substrate By similarity
Binding sitei268 – 2681Substrate By similarity
Binding sitei270 – 2701ATP By similarity
Binding sitei315 – 3151ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi243 – 2464ATP By similarity
Nucleotide bindingi252 – 2543ATP By similarity
Nucleotide bindingi327 – 3293ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid phosphatase activity Source: InterPro
  3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB
  4. inositol hexakisphosphate 1-kinase activity Source: UniProtKB-EC
  5. inositol hexakisphosphate 3-kinase activity Source: UniProtKB-EC
  6. inositol hexakisphosphate 5-kinase activity Source: UniProtKB
  7. inositol-1,3,4,5,6-pentakisphosphate kinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. inositol metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196482. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 (EC:2.7.4.21, EC:2.7.4.24)
Alternative name(s):
Diphosphoinositol pentakisphosphate kinase 2
Histidine acid phosphatase domain-containing protein 1
InsP6 and PP-IP5 kinase 2
VIP1 homolog 2
Short name:
mmVIP2
Gene namesi
Name:Ppip5k2
Synonyms:Hisppd1, Kiaa0433, Vip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2142810. Ppip5k2.

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11291129Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2
PRO_0000315693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Phosphoserine By similarity
Modified residuei1058 – 10581Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6ZQB6.
PaxDbiQ6ZQB6.
PRIDEiQ6ZQB6.

PTM databases

PhosphoSiteiQ6ZQB6.

Expressioni

Gene expression databases

ArrayExpressiQ6ZQB6.
BgeeiQ6ZQB6.
CleanExiMM_HISPPD1.
GenevestigatoriQ6ZQB6.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQB6.
SMRiQ6ZQB6. Positions 48-366.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 602Substrate binding By similarity
Regioni219 – 2202Substrate binding By similarity
Regioni332 – 3354Substrate binding By similarity
Regioni377 – 44872Polyphosphoinositide-binding domain By similarity
Add
BLAST

Domaini

The polyphosphoinositide-binding domain mediates binding of PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG245915.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
HOVERGENiHBG108657.
KOiK13024.
OrthoDBiEOG77M8MT.

Family and domain databases

Gene3Di3.40.50.1240. 4 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 3 hits.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6ZQB6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSNSRKMSEP PRFFVGPEDA EINPGNYRRF FHHAEEEEEE EDESPPERQI     50
VVGICSMAKK SKSKPMKEIL ERISLFKYIT VVVFEEEIIL NEPVENWPLC 100
DCLISFHSKG FPLDKAVAYA KLRNPFVIND LNMQYLIQDR RDVYSILQAE 150
GILLPRYAIL NRDPNNPKEC NLIEGEDHVE VNGEVFQKPF VEKPVSAEDH 200
NVYIYYPTSA GGGSQRLFRK IGSRSSVYSP ESNVRKTGSY IYEEFMPTDG 250
TDVKVYTVGP DYAHAEARKS PALDGKVERD SEGKEVRYPV ILNAREKLIA 300
WKVCLAFKQT VCGFDLLRAN GQSYVCDVNG FSFVKNSMKY YDDCAKILGN 350
IVMRELAPQF HIPWSIPLEA EDIPIVPTTS GTMMELRCVI AVIRHGDRTP 400
KQKMKMEVRH QKFFDLFEKC DGYKSGKLKL KKPKQLQEVL DIARQLLMEL 450
GQNNDSEIEE NKSKLEQLKT VLEMYGHFSG INRKVQLTYL PHGCPKTSSE 500
EEDNRREEPS LLLVLKWGGE LTPAGRVQAE ELGRAFRCMY PGGQGDYAGF 550
PGCGLLRLHS TYRHDLKIYA SDEGRVQMTA AAFAKGLLAL EGELTPILVQ 600
MVKSANMNGL LDSDSDSLSS CQQRVKARLH EILQKDRDFT AEDYEKLTPS 650
GSISVIKSMH LIKNPVKTCD KVYSLIQSLT SQIRYRMEDP KSADIQLYHS 700
ETLELMLRRW SKLEKDFKTK NGRYDISKIP DIYDCIKYDV QHNGSLKLEN 750
TMELYRLSKA LADIVIPQEY GITKAEKLEI AKGYCTPLVR KIRSDLQRTQ 800
DDDTVNKLHP VYSRGVLSPE RHVRTRLYFT SESHVHSLLS ILRYGALCDD 850
SKDEQWKRAM DYLNVVNELN YMTQIVIMLY EDPNKDLSSE ERFHVELHFS 900
PGAKGCEEDK NLPSGYGYRP ASRENEGRRS LKTDDDEPHT SKRDEVDRAV 950
MLFKPLVSEP IHIHRKSPLP RSRKITANEV VSENANYLRT PRNLVEQKQN 1000
PTVGFELYSM VPSICPLETL HNALFLKQVD DFLASIASPS TEVLRKVPEM 1050
SSMATRSSPG MRRKISLNTY TPTKILPTPP AALKSSKASS KAAAGGPSQA 1100
MAPHTSSRKK SINSKTEGHE PKKSTGKKR 1129
Length:1,129
Mass (Da):128,427
Last modified:July 27, 2011 - v3
Checksum:iA563826CC0085E3C
GO
Isoform 2 (identifier: Q6ZQB6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     545-545: Missing.
     1051-1129: SSMATRSSPG...EPKKSTGKKR → CMEFTFIVT

Note: No experimental confirmation available.

Show »
Length:1,058
Mass (Da):121,181
Checksum:iEAD1A1398DB250E7
GO
Isoform 3 (identifier: Q6ZQB6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:1,123
Mass (Da):127,723
Checksum:i7B7941A5E5AB948F
GO

Sequence cautioni

The sequence BAC97950.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66Missing in isoform 3.
VSP_041618
Alternative sequencei545 – 5451Missing in isoform 2.
VSP_030637
Alternative sequencei1051 – 112979SSMAT…TGKKR → CMEFTFIVT in isoform 2.
VSP_030638Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971A → V in BAE38567. 1 Publication
Sequence conflicti582 – 5821A → T in AAH53396. 1 Publication
Sequence conflicti729 – 7291I → V in AAH53396. 1 Publication
Sequence conflicti1035 – 10351S → P in AAH53396. 1 Publication
Sequence conflicti1109 – 11091K → M in BAC97950. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129140 mRNA. Translation: BAC97950.1. Different initiation.
AK138622 mRNA. Translation: BAE23724.1.
AK166095 mRNA. Translation: BAE38567.1.
AC099860 Genomic DNA. No translation available.
AC162298 Genomic DNA. No translation available.
BC053396 mRNA. Translation: AAH53396.1.
CCDSiCCDS35678.1. [Q6ZQB6-1]
RefSeqiNP_776121.4. NM_173760.5. [Q6ZQB6-1]
UniGeneiMm.220817.
Mm.417682.

Genome annotation databases

EnsembliENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648. [Q6ZQB6-3]
ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648. [Q6ZQB6-3]
GeneIDi227399.
KEGGimmu:227399.
UCSCiuc007cfk.2. mouse. [Q6ZQB6-1]
uc007cfl.1. mouse. [Q6ZQB6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129140 mRNA. Translation: BAC97950.1 . Different initiation.
AK138622 mRNA. Translation: BAE23724.1 .
AK166095 mRNA. Translation: BAE38567.1 .
AC099860 Genomic DNA. No translation available.
AC162298 Genomic DNA. No translation available.
BC053396 mRNA. Translation: AAH53396.1 .
CCDSi CCDS35678.1. [Q6ZQB6-1 ]
RefSeqi NP_776121.4. NM_173760.5. [Q6ZQB6-1 ]
UniGenei Mm.220817.
Mm.417682.

3D structure databases

ProteinModelPortali Q6ZQB6.
SMRi Q6ZQB6. Positions 48-366.
ModBasei Search...

PTM databases

PhosphoSitei Q6ZQB6.

Proteomic databases

MaxQBi Q6ZQB6.
PaxDbi Q6ZQB6.
PRIDEi Q6ZQB6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042509 ; ENSMUSP00000043401 ; ENSMUSG00000040648 . [Q6ZQB6-3 ]
ENSMUST00000171129 ; ENSMUSP00000132889 ; ENSMUSG00000040648 . [Q6ZQB6-3 ]
GeneIDi 227399.
KEGGi mmu:227399.
UCSCi uc007cfk.2. mouse. [Q6ZQB6-1 ]
uc007cfl.1. mouse. [Q6ZQB6-2 ]

Organism-specific databases

CTDi 23262.
MGIi MGI:2142810. Ppip5k2.
Rougei Search...

Phylogenomic databases

eggNOGi NOG245915.
GeneTreei ENSGT00390000009048.
HOGENOMi HOG000177917.
HOVERGENi HBG108657.
KOi K13024.
OrthoDBi EOG77M8MT.

Enzyme and pathway databases

Reactomei REACT_196482. Synthesis of pyrophosphates in the cytosol.

Miscellaneous databases

NextBioi 378596.
PROi Q6ZQB6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6ZQB6.
Bgeei Q6ZQB6.
CleanExi MM_HISPPD1.
Genevestigatori Q6ZQB6.

Family and domain databases

Gene3Di 3.40.50.1240. 4 hits.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 3 hits.
PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Lung and Spinal cord.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Egg.
  5. "Cloning and characterization of two human VIP1-like inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinases."
    Fridy P.C., Otto J.C., Dollins D.E., York J.D.
    J. Biol. Chem. 282:30754-30762(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVIP2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQB6
Secondary accession number(s): E9PVE9
, Q3TM75, Q3UUA3, Q7TPU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi