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Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2

Gene

Ppip5k2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.1 Publication

Kineticsi

  1. KM=0.23 µM for InsP61 Publication
  2. KM=0.54 µM for InsP71 Publication
  1. Vmax=1.70 nmol/min/mg enzyme with InsP6 as substrate1 Publication
  2. Vmax=5.23 nmol/min/mg enzyme with InsP7 as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140ATPBy similarity1
Binding sitei193ATPBy similarity1
Binding sitei200ATPBy similarity1
Binding sitei219ATPBy similarity1
Binding sitei254SubstrateBy similarity1
Binding sitei268SubstrateBy similarity1
Binding sitei270ATPBy similarity1
Binding sitei315ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi243 – 246ATPBy similarity4
Nucleotide bindingi252 – 254ATPBy similarity3
Nucleotide bindingi327 – 329ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 (EC:2.7.4.211 Publication, EC:2.7.4.241 Publication)
Alternative name(s):
Diphosphoinositol pentakisphosphate kinase 2
Histidine acid phosphatase domain-containing protein 1
InsP6 and PP-IP5 kinase 2
VIP1 homolog 2
Short name:
mmVIP2
Gene namesi
Name:Ppip5k2
Synonyms:Hisppd1, Kiaa0433, Vip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2142810. Ppip5k2.

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156931 – 1129Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei229PhosphoserineBy similarity1
Modified residuei1051PhosphoserineBy similarity1
Modified residuei1058PhosphoserineBy similarity1
Modified residuei1066PhosphoserineCombined sources1
Modified residuei1106PhosphoserineBy similarity1
Modified residuei1107PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6ZQB6.
PeptideAtlasiQ6ZQB6.
PRIDEiQ6ZQB6.

PTM databases

iPTMnetiQ6ZQB6.
PhosphoSitePlusiQ6ZQB6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040648.
CleanExiMM_HISPPD1.
ExpressionAtlasiQ6ZQB6. baseline and differential.
GenevisibleiQ6ZQB6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043401.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQB6.
SMRiQ6ZQB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 60Substrate bindingBy similarity2
Regioni219 – 220Substrate bindingBy similarity2
Regioni332 – 335Substrate bindingBy similarity4
Regioni377 – 448Polyphosphoinositide-binding domainBy similarityAdd BLAST72

Domaini

The C-terminal acid phosphatase-like domain binds PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1057. Eukaryota.
ENOG410XNSN. LUCA.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
HOVERGENiHBG108657.
InParanoidiQ6ZQB6.
KOiK13024.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 4 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 3 hits.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZQB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNSRKMSEP PRFFVGPEDA EINPGNYRRF FHHAEEEEEE EDESPPERQI
60 70 80 90 100
VVGICSMAKK SKSKPMKEIL ERISLFKYIT VVVFEEEIIL NEPVENWPLC
110 120 130 140 150
DCLISFHSKG FPLDKAVAYA KLRNPFVIND LNMQYLIQDR RDVYSILQAE
160 170 180 190 200
GILLPRYAIL NRDPNNPKEC NLIEGEDHVE VNGEVFQKPF VEKPVSAEDH
210 220 230 240 250
NVYIYYPTSA GGGSQRLFRK IGSRSSVYSP ESNVRKTGSY IYEEFMPTDG
260 270 280 290 300
TDVKVYTVGP DYAHAEARKS PALDGKVERD SEGKEVRYPV ILNAREKLIA
310 320 330 340 350
WKVCLAFKQT VCGFDLLRAN GQSYVCDVNG FSFVKNSMKY YDDCAKILGN
360 370 380 390 400
IVMRELAPQF HIPWSIPLEA EDIPIVPTTS GTMMELRCVI AVIRHGDRTP
410 420 430 440 450
KQKMKMEVRH QKFFDLFEKC DGYKSGKLKL KKPKQLQEVL DIARQLLMEL
460 470 480 490 500
GQNNDSEIEE NKSKLEQLKT VLEMYGHFSG INRKVQLTYL PHGCPKTSSE
510 520 530 540 550
EEDNRREEPS LLLVLKWGGE LTPAGRVQAE ELGRAFRCMY PGGQGDYAGF
560 570 580 590 600
PGCGLLRLHS TYRHDLKIYA SDEGRVQMTA AAFAKGLLAL EGELTPILVQ
610 620 630 640 650
MVKSANMNGL LDSDSDSLSS CQQRVKARLH EILQKDRDFT AEDYEKLTPS
660 670 680 690 700
GSISVIKSMH LIKNPVKTCD KVYSLIQSLT SQIRYRMEDP KSADIQLYHS
710 720 730 740 750
ETLELMLRRW SKLEKDFKTK NGRYDISKIP DIYDCIKYDV QHNGSLKLEN
760 770 780 790 800
TMELYRLSKA LADIVIPQEY GITKAEKLEI AKGYCTPLVR KIRSDLQRTQ
810 820 830 840 850
DDDTVNKLHP VYSRGVLSPE RHVRTRLYFT SESHVHSLLS ILRYGALCDD
860 870 880 890 900
SKDEQWKRAM DYLNVVNELN YMTQIVIMLY EDPNKDLSSE ERFHVELHFS
910 920 930 940 950
PGAKGCEEDK NLPSGYGYRP ASRENEGRRS LKTDDDEPHT SKRDEVDRAV
960 970 980 990 1000
MLFKPLVSEP IHIHRKSPLP RSRKITANEV VSENANYLRT PRNLVEQKQN
1010 1020 1030 1040 1050
PTVGFELYSM VPSICPLETL HNALFLKQVD DFLASIASPS TEVLRKVPEM
1060 1070 1080 1090 1100
SSMATRSSPG MRRKISLNTY TPTKILPTPP AALKSSKASS KAAAGGPSQA
1110 1120
MAPHTSSRKK SINSKTEGHE PKKSTGKKR
Length:1,129
Mass (Da):128,427
Last modified:July 27, 2011 - v3
Checksum:iA563826CC0085E3C
GO
Isoform 2 (identifier: Q6ZQB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     545-545: Missing.
     1051-1129: SSMATRSSPG...EPKKSTGKKR → CMEFTFIVT

Note: No experimental confirmation available.
Show »
Length:1,058
Mass (Da):121,181
Checksum:iEAD1A1398DB250E7
GO
Isoform 3 (identifier: Q6ZQB6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:1,123
Mass (Da):127,723
Checksum:i7B7941A5E5AB948F
GO

Sequence cautioni

The sequence BAC97950 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197A → V in BAE38567 (PubMed:14621295).Curated1
Sequence conflicti582A → T in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti729I → V in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti1035S → P in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti1109K → M in BAC97950 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0416181 – 6Missing in isoform 3. 1 Publication6
Alternative sequenceiVSP_030637545Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_0306381051 – 1129SSMAT…TGKKR → CMEFTFIVT in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129140 mRNA. Translation: BAC97950.1. Different initiation.
AK138622 mRNA. Translation: BAE23724.1.
AK166095 mRNA. Translation: BAE38567.1.
AC099860 Genomic DNA. No translation available.
AC162298 Genomic DNA. No translation available.
BC053396 mRNA. Translation: AAH53396.1.
CCDSiCCDS35678.2. [Q6ZQB6-1]
RefSeqiNP_776121.4. NM_173760.5. [Q6ZQB6-1]
UniGeneiMm.220817.
Mm.417682.

Genome annotation databases

EnsembliENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648. [Q6ZQB6-1]
ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648. [Q6ZQB6-3]
GeneIDi227399.
KEGGimmu:227399.
UCSCiuc007cfk.4. mouse. [Q6ZQB6-1]
uc007cfl.1. mouse. [Q6ZQB6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129140 mRNA. Translation: BAC97950.1. Different initiation.
AK138622 mRNA. Translation: BAE23724.1.
AK166095 mRNA. Translation: BAE38567.1.
AC099860 Genomic DNA. No translation available.
AC162298 Genomic DNA. No translation available.
BC053396 mRNA. Translation: AAH53396.1.
CCDSiCCDS35678.2. [Q6ZQB6-1]
RefSeqiNP_776121.4. NM_173760.5. [Q6ZQB6-1]
UniGeneiMm.220817.
Mm.417682.

3D structure databases

ProteinModelPortaliQ6ZQB6.
SMRiQ6ZQB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043401.

PTM databases

iPTMnetiQ6ZQB6.
PhosphoSitePlusiQ6ZQB6.

Proteomic databases

PaxDbiQ6ZQB6.
PeptideAtlasiQ6ZQB6.
PRIDEiQ6ZQB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648. [Q6ZQB6-1]
ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648. [Q6ZQB6-3]
GeneIDi227399.
KEGGimmu:227399.
UCSCiuc007cfk.4. mouse. [Q6ZQB6-1]
uc007cfl.1. mouse. [Q6ZQB6-2]

Organism-specific databases

CTDi23262.
MGIiMGI:2142810. Ppip5k2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1057. Eukaryota.
ENOG410XNSN. LUCA.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
HOVERGENiHBG108657.
InParanoidiQ6ZQB6.
KOiK13024.

Enzyme and pathway databases

ReactomeiR-MMU-1855167. Synthesis of pyrophosphates in the cytosol.

Miscellaneous databases

PROiQ6ZQB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040648.
CleanExiMM_HISPPD1.
ExpressionAtlasiQ6ZQB6. baseline and differential.
GenevisibleiQ6ZQB6. MM.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 4 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 3 hits.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIP2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQB6
Secondary accession number(s): E9PVE9
, Q3TM75, Q3UUA3, Q7TPU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.