Q6ZQ93 (UBP34_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 68.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 34 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 34 Ubiquitin thioesterase 34 Ubiquitin-specific-processing protease 34 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 3582 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with AXIN1 and AXIN2 By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. |
| Sequence caution | The sequence AAH55938.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence CAM20294.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
Alternative products
| This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q6ZQ93-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q6ZQ93-2) The sequence of this isoform differs from the canonical sequence as follows: 1-151: Missing. 152-160: DEKEKLLLC → MRRKNYYYV | ||||||
| Isoform 3 (identifier: Q6ZQ93-3) The sequence of this isoform differs from the canonical sequence as follows: 1-151: Missing. 152-160: DEKEKLLLC → MRRKNYYYV 3124-3206: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 4 (identifier: Q6ZQ93-4) The sequence of this isoform differs from the canonical sequence as follows: 1-151: Missing. 152-160: DEKEKLLLC → MRRKNYYYV 2569-3582: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 3582 | 3582 | Ubiquitin carboxyl-terminal hydrolase 34 | PRO_0000249520 | |||||
Sites | |||||||||
| Active site | 1940 | 1 | Nucleophile By similarity | ||||||
| Active site | 2201 | 1 | Proton acceptor By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 352 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 2525 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 2582 | 1 | Phosphothreonine Ref.7 | ||||||
| Modified residue | 3395 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 3396 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 3443 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 151 | 151 | Missing in isoform 2, isoform 3 and isoform 4. | VSP_035641 | |||||
| Alternative sequence | 152 – 160 | 9 | DEKEKLLLC → MRRKNYYYV in isoform 2, isoform 3 and isoform 4. | VSP_035642 | |||||
| Alternative sequence | 2569 – 3582 | 1014 | Missing in isoform 4. | VSP_020464 | |||||
| Alternative sequence | 3124 – 3206 | 83 | Missing in isoform 3. | VSP_020465 | |||||
Experimental info | |||||||||
| Sequence conflict | 2163 | 1 | K → Q in BAE25365. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 225-639; 2096-2278 AND 2437-3582 (ISOFORM 4). Strain: C57BL/6J. Tissue: Ovary, Sympathetic ganglion and Testis. |
| [3] | "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2279-3582 (ISOFORM 3). Tissue: Embryonic tail. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2346-2569 AND 3160-3376. Strain: C57BL/6 and Czech II. Tissue: Brain and Mammary tumor. |
| [5] | "Mouse U2af1-rs1 is a neomorphic imprinted gene." Nabetani A., Hatada I., Morisaki H., Oshimura M., Mukai T. Mol. Cell. Biol. 17:789-798(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION. |
| [6] | "Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2525, MASS SPECTROMETRY. Tissue: Embryonic brain. |
| [7] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2582, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL672049 Genomic DNA. Translation: CAM16931.1. AL672049 AL928722 Genomic DNA. Translation: CAM16940.1.AL672004 Genomic DNA. Translation: CAM17339.1. AL672004 AL928722 Genomic DNA. Translation: CAM17340.1.AL772359 AL928722 Genomic DNA. Translation: CAM20294.1. Different initiation.AL928722 AL772359 Genomic DNA. Translation: CAO77877.1.AK033182 mRNA. Translation: BAC28186.1. AK143407 mRNA. Translation: BAE25365.1. AK148805 mRNA. Translation: BAE28668.1. AK129165 mRNA. Translation: BAC97975.1. BC055938 mRNA. Translation: AAH55938.1. Sequence problems. BC063062 mRNA. Translation: AAH63062.1. |
| IPI | IPI00649643. IPI00668474. IPI00788320. IPI00914688. |
| RefSeq | NP_001177330.2. NM_001190401.2. |
| UniGene | Mm.119155. |
3D structure databases | |
| ProteinModelPortal | Q6ZQ93. |
| SMR | Q6ZQ93. Positions 1930-2275. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | C19.067. |
PTM databases | |
| PhosphoSite | Q6ZQ93. |
Proteomic databases | |
| PaxDb | Q6ZQ93. |
| PRIDE | Q6ZQ93. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000129368; ENSMUSP00000121426; ENSMUSG00000056342. ENSMUST00000130131; ENSMUSP00000115168; ENSMUSG00000056342. ENSMUST00000180046; ENSMUSP00000137430; ENSMUSG00000056342. |
| GeneID | 17847. |
| KEGG | mmu:17847. |
| UCSC | uc007ieu.2. mouse. |
Organism-specific databases | |
| CTD | 9736. |
| MGI | MGI:109473. Usp34. |
| Rouge | Search... |
Phylogenomic databases | |
| eggNOG | COG5077. |
| GeneTree | ENSGT00650000093229. |
| HOVERGEN | HBG092616. |
| InParanoid | Q6ZQ93. |
| KO | K11853. |
Gene expression databases | |
| ArrayExpress | Q6ZQ93. |
| Bgee | Q6ZQ93. |
| CleanEx | MM_USP34. |
| Genevestigator | Q6ZQ93. |
| GermOnline | ENSMUSG00000056342. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016024. ARM-type_fold. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view] |
| Pfam | PF00443. UCH. 1 hit. [Graphical view] |
| SUPFAM | SSF48371. ARM-type_fold. 1 hit. |
| PROSITE | PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 292585. |
| SOURCE | Search... |
Entry information
| Entry name | UBP34_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q6ZQ93 Secondary accession number(s): A2AF26 Q8CCH0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
