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Protein

E3 ubiquitin-protein ligase MARCH6

Gene

March6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 62RING-CH-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH6 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 6
Membrane-associated RING-CH protein VI
Short name:
MARCH-VI
RING-type E3 ubiquitin transferase MARCH6Curated
Gene namesi
Name:March6
Synonyms:Kiaa0597
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2442773 March6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 91CytoplasmicSequence analysisAdd BLAST91
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21
Topological domaini113 – 142ExtracellularSequence analysisAdd BLAST30
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 283CytoplasmicSequence analysisAdd BLAST120
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 336ExtracellularSequence analysisAdd BLAST32
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 376CytoplasmicSequence analysisAdd BLAST19
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 421ExtracellularSequence analysisAdd BLAST24
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 480CytoplasmicSequence analysisAdd BLAST38
Transmembranei481 – 501HelicalSequence analysisAdd BLAST21
Topological domaini502 – 519ExtracellularSequence analysisAdd BLAST18
Transmembranei520 – 540HelicalSequence analysisAdd BLAST21
Topological domaini541 – 631CytoplasmicSequence analysisAdd BLAST91
Transmembranei632 – 652HelicalSequence analysisAdd BLAST21
Topological domaini653 – 677ExtracellularSequence analysisAdd BLAST25
Transmembranei678 – 698HelicalSequence analysisAdd BLAST21
Topological domaini699 – 720CytoplasmicSequence analysisAdd BLAST22
Transmembranei721 – 741HelicalSequence analysisAdd BLAST21
Topological domaini742 – 763ExtracellularSequence analysisAdd BLAST22
Transmembranei764 – 784HelicalSequence analysisAdd BLAST21
Topological domaini785 – 814CytoplasmicSequence analysisAdd BLAST30
Transmembranei815 – 835HelicalSequence analysisAdd BLAST21
Topological domaini836 – 847ExtracellularSequence analysisAdd BLAST12
Transmembranei848 – 868HelicalSequence analysisAdd BLAST21
Topological domaini869 – 909CytoplasmicSequence analysisAdd BLAST41

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002742991 – 909E3 ubiquitin-protein ligase MARCH6Add BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

Auto-ubiquitinated, which results in proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ6ZQ89
PeptideAtlasiQ6ZQ89
PRIDEiQ6ZQ89

PTM databases

iPTMnetiQ6ZQ89
PhosphoSitePlusiQ6ZQ89

Expressioni

Gene expression databases

BgeeiENSMUSG00000039100
CleanExiMM_MARCH6
GenevisibleiQ6ZQ89 MM

Interactioni

Subunit structurei

Interacts with DIO2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087694

Structurei

3D structure databases

ProteinModelPortaliQ6ZQ89
SMRiQ6ZQ89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the DOA10/MARCH6 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 62RING-CH-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609 Eukaryota
COG5183 LUCA
GeneTreeiENSGT00730000110355
HOGENOMiHOG000286005
HOVERGENiHBG080753
InParanoidiQ6ZQ89
KOiK10661
OMAiWLPIRIL
OrthoDBiEOG091G02GT
PhylomeDBiQ6ZQ89
TreeFamiTF105777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011016 Znf_RING-CH
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF12906 RINGv, 1 hit
SMARTiView protein in SMART
SM00744 RINGv, 1 hit
PROSITEiView protein in PROSITE
PS51292 ZF_RING_CH, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZQ89-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTAEEDICR VCRSEGTPEK PLYHPCVCTG SIKFIHQECL VQWLKHSRKE
60 70 80 90 100
YCELCKHRFA FTPIYSPDMP SRLPIQDIFA GLVTSIGTAI RYWFHYTLVA
110 120 130 140 150
FAWLGVVPLT ACRIYKCLFT GSVSSLLTLP LDMLSTENLL ADCLQGCFVV
160 170 180 190 200
TCTLCAFISL VWLREQIVHG GAPIWLEHAA PPFNAAGHHQ NEAPVGGNGA
210 220 230 240 250
ENPAADQPAN PAGENAVLGE NPDAQDGQAE EEEEDNEEED DAGVEDAADA
260 270 280 290 300
NNGAQDDMNW NALEWDRAAE ELTWERMLGL DGSLVFLEHV FWVVSLNTLF
310 320 330 340 350
ILVFAFCPYH IGHFSLVGLG FEEHVQASHF EGLITTIVGY ILLAITLIIC
360 370 380 390 400
HALATLVKFH RSRRLLGVCY IVVKVSLLVV VEIGVFPLIC GWWLDICSLE
410 420 430 440 450
MFDATLKDRE LSFQSAPGTT MFLHWLVGMV YVFYFASFIL LLREVLRPGV
460 470 480 490 500
LWFLRNLNDP DFNPVQEMIH LPIYRHLRRF ILSVIVFGSI VLLMLWLPIR
510 520 530 540 550
IIKSLLPNFL PYNVMLYSDA PVSELSLELL LLQVVLPALL EQGHTRQWLK
560 570 580 590 600
GLVRAWTVTA GYLLDLHSYL LGDQEENENS ANQQVNNNQP ARNNNAVPAG
610 620 630 640 650
EGLHAAHQAI LQQGGPVGFQ PYRRPLNFPL RIFLLIVFMC ITLLIASLIC
660 670 680 690 700
LTLPVFAGRW LMSFWTGTAK IHELYTAACG LYVCWLTIRA VTVLVAWMPQ
710 720 730 740 750
GRRVIFQKVK EWSLMIMKTL IVAVLLAGVV PLLLGLLFEL VIVAPLRVPL
760 770 780 790 800
DQTPLFYPWQ DWALGVLHAK IIAAITLMGP QWWLKTVIEQ VYANGIRNID
810 820 830 840 850
LHYIIRKLAA PVISVLLLSL CVPYVIASGA VPLLGVTAEM QNLVHRRIYP
860 870 880 890 900
FLLMVVVLMG ILSFQVRQFK RLYEHIKNDK YLVGQRLVNY ERKSGKQGPS

TPPPVSSQE
Length:909
Mass (Da):102,273
Last modified:February 6, 2007 - v2
Checksum:iDC46CB2A34B6F749
GO
Isoform 2 (identifier: Q6ZQ89-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     625-821: PLNFPLRIFL...VISVLLLSLC → LPRWAPSHWQ...SWGFIVHNIC
     822-909: Missing.

Note: No experimental confirmation available.
Show »
Length:661
Mass (Da):74,274
Checksum:iC73C562C44B0DAF4
GO
Isoform 3 (identifier: Q6ZQ89-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     683-708: Missing.

Note: No experimental confirmation available.
Show »
Length:883
Mass (Da):99,219
Checksum:i5DB1C80612C77827
GO

Sequence cautioni

The sequence AAH37454 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti476H → N in BAC31427 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022702625 – 821PLNFP…LLSLC → LPRWAPSHWQGLLSCVLCPV AFRKMMSSWGFIVHNIC in isoform 2. 1 PublicationAdd BLAST197
Alternative sequenceiVSP_022704683 – 708Missing in isoform 3. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_022703822 – 909Missing in isoform 2. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042980 mRNA Translation: BAC31427.1
AK089827 mRNA Translation: BAC40971.1
AK129169 Transcribed RNA Translation: BAC97979.1
BC037454 mRNA Translation: AAH37454.1 Different initiation.
BC048816 mRNA Translation: AAH48816.1
BC059190 mRNA Translation: AAH59190.2
CCDSiCCDS37054.1 [Q6ZQ89-1]
RefSeqiNP_766194.2, NM_172606.2 [Q6ZQ89-1]
UniGeneiMm.272185

Genome annotation databases

EnsembliENSMUST00000090227; ENSMUSP00000087694; ENSMUSG00000039100 [Q6ZQ89-1]
GeneIDi223455
KEGGimmu:223455
UCSCiuc007vkh.1 mouse [Q6ZQ89-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMARH6_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQ89
Secondary accession number(s): Q6PCS1
, Q80V02, Q80VC7, Q8BJA0, Q8BXX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health