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Q6ZQ89

- MARH6_MOUSE

UniProt

Q6ZQ89 - MARH6_MOUSE

Protein

E3 ubiquitin-protein ligase MARCH6

Gene

March6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1 By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 6262RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: Ensembl
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein K48-linked ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase MARCH6 (EC:6.3.2.-)
    Alternative name(s):
    Membrane-associated RING finger protein 6
    Membrane-associated RING-CH protein VI
    Short name:
    MARCH-VI
    Gene namesi
    Name:March6
    Synonyms:Kiaa0597
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2442773. March6.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 909909E3 ubiquitin-protein ligase MARCH6PRO_0000274299Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Auto-ubiquitinated, which results in proteasomal degradation.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PaxDbiQ6ZQ89.
    PRIDEiQ6ZQ89.

    PTM databases

    PhosphoSiteiQ6ZQ89.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6ZQ89.
    BgeeiQ6ZQ89.
    CleanExiMM_MARCH6.
    GenevestigatoriQ6ZQ89.

    Interactioni

    Subunit structurei

    Interacts with DIO2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6ZQ89.
    SMRiQ6ZQ89. Positions 4-59.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9191CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini113 – 14230ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini164 – 283120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini305 – 33632ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini358 – 37619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini398 – 42124ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini443 – 48038CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini502 – 51918ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini541 – 63191CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini653 – 67725ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini699 – 72022CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini742 – 76322ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini785 – 81430CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini836 – 84712ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini869 – 90941CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei92 – 11221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei143 – 16321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei284 – 30421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei337 – 35721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei377 – 39721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei422 – 44221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei481 – 50121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei520 – 54021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei632 – 65221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei678 – 69821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei721 – 74121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei764 – 78421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei815 – 83521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei848 – 86821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the DOA10/MARCH6 family.Curated
    Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 6262RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5183.
    GeneTreeiENSGT00730000110355.
    HOGENOMiHOG000286005.
    HOVERGENiHBG080753.
    InParanoidiQ6ZQ89.
    KOiK10661.
    OMAiDNLLADC.
    OrthoDBiEOG7J4464.
    PhylomeDBiQ6ZQ89.
    TreeFamiTF105777.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12906. RINGv. 1 hit.
    [Graphical view]
    SMARTiSM00744. RINGv. 1 hit.
    [Graphical view]
    PROSITEiPS51292. ZF_RING_CH. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6ZQ89-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDTAEEDICR VCRSEGTPEK PLYHPCVCTG SIKFIHQECL VQWLKHSRKE    50
    YCELCKHRFA FTPIYSPDMP SRLPIQDIFA GLVTSIGTAI RYWFHYTLVA 100
    FAWLGVVPLT ACRIYKCLFT GSVSSLLTLP LDMLSTENLL ADCLQGCFVV 150
    TCTLCAFISL VWLREQIVHG GAPIWLEHAA PPFNAAGHHQ NEAPVGGNGA 200
    ENPAADQPAN PAGENAVLGE NPDAQDGQAE EEEEDNEEED DAGVEDAADA 250
    NNGAQDDMNW NALEWDRAAE ELTWERMLGL DGSLVFLEHV FWVVSLNTLF 300
    ILVFAFCPYH IGHFSLVGLG FEEHVQASHF EGLITTIVGY ILLAITLIIC 350
    HALATLVKFH RSRRLLGVCY IVVKVSLLVV VEIGVFPLIC GWWLDICSLE 400
    MFDATLKDRE LSFQSAPGTT MFLHWLVGMV YVFYFASFIL LLREVLRPGV 450
    LWFLRNLNDP DFNPVQEMIH LPIYRHLRRF ILSVIVFGSI VLLMLWLPIR 500
    IIKSLLPNFL PYNVMLYSDA PVSELSLELL LLQVVLPALL EQGHTRQWLK 550
    GLVRAWTVTA GYLLDLHSYL LGDQEENENS ANQQVNNNQP ARNNNAVPAG 600
    EGLHAAHQAI LQQGGPVGFQ PYRRPLNFPL RIFLLIVFMC ITLLIASLIC 650
    LTLPVFAGRW LMSFWTGTAK IHELYTAACG LYVCWLTIRA VTVLVAWMPQ 700
    GRRVIFQKVK EWSLMIMKTL IVAVLLAGVV PLLLGLLFEL VIVAPLRVPL 750
    DQTPLFYPWQ DWALGVLHAK IIAAITLMGP QWWLKTVIEQ VYANGIRNID 800
    LHYIIRKLAA PVISVLLLSL CVPYVIASGA VPLLGVTAEM QNLVHRRIYP 850
    FLLMVVVLMG ILSFQVRQFK RLYEHIKNDK YLVGQRLVNY ERKSGKQGPS 900
    TPPPVSSQE 909
    Length:909
    Mass (Da):102,273
    Last modified:February 6, 2007 - v2
    Checksum:iDC46CB2A34B6F749
    GO
    Isoform 2 (identifier: Q6ZQ89-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         625-821: PLNFPLRIFL...VISVLLLSLC → LPRWAPSHWQ...SWGFIVHNIC
         822-909: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:661
    Mass (Da):74,274
    Checksum:iC73C562C44B0DAF4
    GO
    Isoform 3 (identifier: Q6ZQ89-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         683-708: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:883
    Mass (Da):99,219
    Checksum:i5DB1C80612C77827
    GO

    Sequence cautioni

    The sequence AAH37454.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti476 – 4761H → N in BAC31427. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei625 – 821197PLNFP…LLSLC → LPRWAPSHWQGLLSCVLCPV AFRKMMSSWGFIVHNIC in isoform 2. 1 PublicationVSP_022702Add
    BLAST
    Alternative sequencei683 – 70826Missing in isoform 3. 1 PublicationVSP_022704Add
    BLAST
    Alternative sequencei822 – 90988Missing in isoform 2. 1 PublicationVSP_022703Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK042980 mRNA. Translation: BAC31427.1.
    AK089827 mRNA. Translation: BAC40971.1.
    AK129169 Transcribed RNA. Translation: BAC97979.1.
    BC037454 mRNA. Translation: AAH37454.1. Different initiation.
    BC048816 mRNA. Translation: AAH48816.1.
    BC059190 mRNA. Translation: AAH59190.2.
    CCDSiCCDS37054.1. [Q6ZQ89-1]
    RefSeqiNP_766194.2. NM_172606.2. [Q6ZQ89-1]
    UniGeneiMm.272185.

    Genome annotation databases

    EnsembliENSMUST00000090227; ENSMUSP00000087694; ENSMUSG00000039100. [Q6ZQ89-1]
    GeneIDi223455.
    KEGGimmu:223455.
    UCSCiuc007vkh.1. mouse. [Q6ZQ89-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK042980 mRNA. Translation: BAC31427.1 .
    AK089827 mRNA. Translation: BAC40971.1 .
    AK129169 Transcribed RNA. Translation: BAC97979.1 .
    BC037454 mRNA. Translation: AAH37454.1 . Different initiation.
    BC048816 mRNA. Translation: AAH48816.1 .
    BC059190 mRNA. Translation: AAH59190.2 .
    CCDSi CCDS37054.1. [Q6ZQ89-1 ]
    RefSeqi NP_766194.2. NM_172606.2. [Q6ZQ89-1 ]
    UniGenei Mm.272185.

    3D structure databases

    ProteinModelPortali Q6ZQ89.
    SMRi Q6ZQ89. Positions 4-59.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6ZQ89.

    Proteomic databases

    PaxDbi Q6ZQ89.
    PRIDEi Q6ZQ89.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090227 ; ENSMUSP00000087694 ; ENSMUSG00000039100 . [Q6ZQ89-1 ]
    GeneIDi 223455.
    KEGGi mmu:223455.
    UCSCi uc007vkh.1. mouse. [Q6ZQ89-1 ]

    Organism-specific databases

    CTDi 10299.
    MGIi MGI:2442773. March6.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5183.
    GeneTreei ENSGT00730000110355.
    HOGENOMi HOG000286005.
    HOVERGENi HBG080753.
    InParanoidi Q6ZQ89.
    KOi K10661.
    OMAi DNLLADC.
    OrthoDBi EOG7J4464.
    PhylomeDBi Q6ZQ89.
    TreeFami TF105777.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi MARCH6. mouse.
    NextBioi 376726.
    PROi Q6ZQ89.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6ZQ89.
    Bgeei Q6ZQ89.
    CleanExi MM_MARCH6.
    Genevestigatori Q6ZQ89.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12906. RINGv. 1 hit.
    [Graphical view ]
    SMARTi SM00744. RINGv. 1 hit.
    [Graphical view ]
    PROSITEi PS51292. ZF_RING_CH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 476-909 (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Cerebellum and Spleen.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 348-883 (ISOFORM 3).
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 383-909 (ISOFORM 1).
      Strain: Czech II and FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiMARH6_MOUSE
    AccessioniPrimary (citable) accession number: Q6ZQ89
    Secondary accession number(s): Q6PCS1
    , Q80V02, Q80VC7, Q8BJA0, Q8BXX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3