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Protein

Ninein-like protein

Gene

Ninl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi247 – 258PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Ninein-like protein
Gene namesi
Name:Ninl
Synonyms:Kiaa0980, Nlp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1925427. Ninl.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasm By similarity

  • Note: In interphase cells, NLP is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NLP resulting in its release into the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002597151 – 1394Ninein-like proteinAdd BLAST1394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.By similarity
Ubiquitinated by the APC/C complex leading to its degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ6ZQ12.
PaxDbiQ6ZQ12.
PRIDEiQ6ZQ12.

PTM databases

iPTMnetiQ6ZQ12.
PhosphoSitePlusiQ6ZQ12.

Expressioni

Gene expression databases

BgeeiENSMUSG00000068115.
CleanExiMM_4930519N13RIK.
ExpressionAtlasiQ6ZQ12. baseline and differential.
GenevisibleiQ6ZQ12. MM.

Interactioni

Subunit structurei

Interacts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Interacts with LCA5 and USH2A (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105522.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQ12.
SMRiQ6ZQ12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini42 – 77EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini197 – 232EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini234 – 269EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili382 – 423Sequence analysisAdd BLAST42
Coiled coili461 – 515Sequence analysisAdd BLAST55
Coiled coili544 – 584Sequence analysisAdd BLAST41
Coiled coili835 – 863Sequence analysisAdd BLAST29
Coiled coili1057 – 1229Sequence analysisAdd BLAST173
Coiled coili1269 – 1331Sequence analysisAdd BLAST63

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi494 – 496KEN box3
Motifi632 – 640D-box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi295 – 298Poly-Thr4

Domaini

The KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.By similarity

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IEH8. Eukaryota.
ENOG41128SZ. LUCA.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000068210.
InParanoidiQ6ZQ12.
KOiK16477.
OMAiGIQNGRE.
OrthoDBiEOG091G00XA.
PhylomeDBiQ6ZQ12.
TreeFamiTF325139.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q6ZQ12-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDNEEENHYV SRLRDVYSSC DTTGTGFLDQ EELTQLCTKL GLEEQLPALL
60 70 80 90 100
HILLGDDRLA RVNFEEFKEG FVAVLSSGSG VEPSDEEGSS SESATSCAVP
110 120 130 140 150
PKYMSGSKWY GRRSLPELGD SATATKYGSE QQAKGSVKPP LRRSASLESV
160 170 180 190 200
ESLKSDEDAE SAKEPQNELF EAQGQLRSWG CEVFGTLRKS CSPSFSTPEN
210 220 230 240 250
LVQGIWHELG IGSSGHLNEQ ELAVVCRSIG LHSLEKQELE ELFSKLDQDG
260 270 280 290 300
DGRVSLAEFQ LGLFGHEPPS LPASSSLIKP NRLWSHYQEE SGCHTTTTSS
310 320 330 340 350
LVSVCSGLRL FSSVDDGSGF AFPEQVISAW AQEGIQNGRE ILQSLDFSVD
360 370 380 390 400
EKVNLLELTW ALDNELLTVD GVIQQAALAC YRQELSYHQG QVDQLVQERD
410 420 430 440 450
KARQDLEKAE KRNLDFVREM DDCHSALEQL TEKKIKHLEQ EYRGRLSLLR
460 470 480 490 500
SEVEMERELF WEQARRQRAV LEQDVGRLQA EETSLREKLT LALKENSRLQ
510 520 530 540 550
KEIIEVVEKL SDSEKLVLRL QSDLQFVLKD KLEPQSMELL AQEEQFTAIL
560 570 580 590 600
NDYELKCRDL QDRNDELQAE LEGLRLRLPR SRQSPAGTPG THRRRIPGRG
610 620 630 640 650
PADNLFVGES TPVSLETEIM VEQMKEHYQE LRMQLETKVN YYEKEIEVMK
660 670 680 690 700
RNFEKDKKEM EQAFQLEVSV LEGQKADLEA LYAKSQEVIL GLKEQLQDAA
710 720 730 740 750
QSPEPAPAGL AHCCAQALCT LAQRLEVEMH LRHQDQLLQI RQEAEEELNQ
760 770 780 790 800
KLSWLEAQHA ACCESLSLQH QCEKDQLLQT HLQRVKDLAA QLDLEKGRRE
810 820 830 840 850
EREQEVLAHC RRQQLKLQAV MSEEQARICR SFTLEKEKLE QTYREQVEGL
860 870 880 890 900
VQEADVLRAL LKNGTTVVSD QQERTPSSMS LGPDSRQQPT ARQAVSPDGR
910 920 930 940 950
TGAPAEWPGP EKAEGRDFPG QLCSIDAMPS PTPTLLSRRS SENLGVRDNH
960 970 980 990 1000
QRPLNAEEGA IPKEPEPSAR TLTGQGQKLP LPVHPQMLEP SLGTTALDRK
1010 1020 1030 1040 1050
AASVGVQGQA SEGPVGDGEG VQEAWLQFRG EATRMRPSLP CSELPNPQEA
1060 1070 1080 1090 1100
TVMPAMSESE MKDVKIKLLQ LEDVVRALEK ADSRESYRAE LQRLSEENLV
1110 1120 1130 1140 1150
LKSDLGKIQL ELETSESKNE VQRQEIEVLK RDKEQACCDL EELSTQTQKY
1160 1170 1180 1190 1200
KDEMSQLNCR VLQLEGEPSG LHTQKEENHG AIQVLMKKLE EAGCREEQQG
1210 1220 1230 1240 1250
DQIQNLKIEL ERVNEECQYL RLSQAELTES LEESRSQLYS VQLRLEAAQS
1260 1270 1280 1290 1300
QHGRIVQRLQ EQMSQLVPGA RVAELQHLLN VKEEEARRLS AQQEEYRQQL
1310 1320 1330 1340 1350
KAREDQVEDA EARLRNVEWL LQEKVEELRK QFEKNTRSDL LLKELYVENA
1360 1370 1380 1390
HLMKAVQLTE EKQRGAEKKN CVLEEKVRAL NKLISKMAPA SLSV
Note: Gene prediction based on EST data.
Length:1,394
Mass (Da):157,838
Last modified:September 1, 2009 - v3
Checksum:i76025DAD6E783320
GO
Isoform 1 (identifier: Q6ZQ12-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     586-703: Missing.

Note: No experimental confirmation available.
Show »
Length:1,276
Mass (Da):144,302
Checksum:i69170C4DCB529B42
GO
Isoform 2 (identifier: Q6ZQ12-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     990-1197: Missing.

Note: No experimental confirmation available.
Show »
Length:1,186
Mass (Da):134,602
Checksum:iBC12BB1FE5EB2DF9
GO

Sequence cautioni

The sequence BAC98063 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177R → Q in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti742Q → R in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti798R → Q in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti920G → D in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti979L → P in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti1066I → T in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti1236S → G in BAC98063 (PubMed:14621295).Curated1
Sequence conflicti1330K → E in BAC98063 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037882586 – 703Missing in isoform 1. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_021526990 – 1197Missing in isoform 2. 1 PublicationAdd BLAST208

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129253 mRNA. Translation: BAC98063.1. Different initiation.
AL808125 Genomic DNA. Translation: CAM17134.1.
BC067071 mRNA. Translation: AAH67071.1.
CCDSiCCDS50744.1. [Q6ZQ12-3]
RefSeqiNP_997087.2. NM_207204.2. [Q6ZQ12-3]
UniGeneiMm.120911.

Genome annotation databases

EnsembliENSMUST00000109896; ENSMUSP00000105522; ENSMUSG00000068115. [Q6ZQ12-3]
GeneIDi78177.
KEGGimmu:78177.
UCSCiuc008mut.2. mouse. [Q6ZQ12-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129253 mRNA. Translation: BAC98063.1. Different initiation.
AL808125 Genomic DNA. Translation: CAM17134.1.
BC067071 mRNA. Translation: AAH67071.1.
CCDSiCCDS50744.1. [Q6ZQ12-3]
RefSeqiNP_997087.2. NM_207204.2. [Q6ZQ12-3]
UniGeneiMm.120911.

3D structure databases

ProteinModelPortaliQ6ZQ12.
SMRiQ6ZQ12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105522.

PTM databases

iPTMnetiQ6ZQ12.
PhosphoSitePlusiQ6ZQ12.

Proteomic databases

MaxQBiQ6ZQ12.
PaxDbiQ6ZQ12.
PRIDEiQ6ZQ12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109896; ENSMUSP00000105522; ENSMUSG00000068115. [Q6ZQ12-3]
GeneIDi78177.
KEGGimmu:78177.
UCSCiuc008mut.2. mouse. [Q6ZQ12-3]

Organism-specific databases

CTDi22981.
MGIiMGI:1925427. Ninl.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IEH8. Eukaryota.
ENOG41128SZ. LUCA.
GeneTreeiENSGT00660000095541.
HOGENOMiHOG000068210.
InParanoidiQ6ZQ12.
KOiK16477.
OMAiGIQNGRE.
OrthoDBiEOG091G00XA.
PhylomeDBiQ6ZQ12.
TreeFamiTF325139.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiNinl. mouse.
PROiQ6ZQ12.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068115.
CleanExiMM_4930519N13RIK.
ExpressionAtlasiQ6ZQ12. baseline and differential.
GenevisibleiQ6ZQ12. MM.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNINL_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQ12
Secondary accession number(s): A2ANB8, Q6NXH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.