Reviewed, UniProtKB/Swiss-Prot Q6ZQ11 (CHSS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Chondroitin sulfate synthase 1
    EC=2.4.1.175
    EC=2.4.1.226
Alternative name(s):
    Chondroitin synthase 1
      Short name=ChSy-1
    Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1
    Chondroitin glucuronyltransferase 1
    N-acetylgalactosaminyltransferase 1

Gene names

Name:	Chsy1
Synonyms:	Kiaa0990

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Cofactor	Divalent cations. Highest activities are measured with cobalt, manganese and cadmium By similarity.
Subunit structure	Binds CHPF By similarity.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 800	800	Chondroitin sulfate synthase 1		PRO_0000189559
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 800	772	Lumenal Potential
Sites
	Metal binding	632	1	Divalent metal cation Potential
	Metal binding	746	1	Divalent metal cation Potential
Amino acid modifications
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	622	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6ZQ11-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: F3DFBC6E7C78C21D	FASTA	800	91,383
	10 20 30 40 50 60 MAARGRRAWL SMLLGLVLGF VLASRLVLPR ASELKRVGPR RRPSPEGCRP GQEASQPGGA 70 80 90 100 110 120 RGDARGAQLW PQGSAAEGVP RDRNFLFVGV MTAQKYLQTR AVAAYRTWSK TIPGKVEFFS 130 140 150 160 170 180 SEGSDTSIPI PVVPLRGVDD SYPPQKKSFM MLKYMHDHYL DKYEWFMRAD DDVYIKGDRL 190 200 210 220 230 240 ESFLRSLNSS EPLFLGQTGL GTTEEMGKLA LEPGENFCMG GPGVILSREV LRRMAPHIGK 250 260 270 280 290 300 CLREMYTTHE DVEVGRCVRR FAGVQCVWSY EMQQLFYENY EQNKKGYIRD LHSSKIHRAI 310 320 330 340 350 360 TLHPNKNPPY QYRLHSYMLS RKIAELRHRT IQLHREIVLM SKYSSTEIQK EDLQLGIPPS 370 380 390 400 410 420 FMRFQARQRE EILEWEFLTG KYLYSATDGQ PPRRGMDSAQ REALDDIVMQ VMEMINANAK 430 440 450 460 470 480 TRGRIIDFKE IQYGYRRVNP MYGAEYILDL LLLYKKHKGK KMTVPVRRHA YLQQTFSKMQ 490 500 510 520 530 540 FVEHEELDAQ ELADRINQDS GSLSFLSNSL KKLVAFQLPG SKTEHKEPKE KKINILIPLS 550 560 570 580 590 600 GRFDMFVRFM GNFEKTCLIP NLNVKLVILL FNSDSNPDKA KQVELMRDYR VKYPKADMQV 610 620 630 640 650 660 LPVSGGFSRA LALEVGSSQF NNESLLFFCD VDLVFTAEFL QRCRANTVLG QQIYFPIIFS 670 680 690 700 710 720 QYDPKIVYSG KVPSDNHFAF TQKTGFWRNY GFGITCIYKG DLVRVGGFDV SIQGWGLEDV 730 740 750 760 770 780 DLFNKVVQAG LKTFRSQEVG VVHIHHPVVC DPNLDPKQYK MCLGSKASTF GSTQQLAEMW 790 800 LEKNDPSYSK GGSHGSARTA

References

[1]

"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic tail.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK129255 mRNA. Translation: BAC98065.1. Different initiation.
IPI	IPI00420540.
RefSeq	NP_001074632.1.
UniGene	Mm.333916
3D structure databases
SMR	Q6ZQ11. Positions 319-351, 527-748.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6ZQ11.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31. GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	Q6ZQ11.
Proteomic databases
PRIDE	Q6ZQ11.
Genome annotation databases
Ensembl	ENSMUST00000036372; ENSMUSP00000047487; ENSMUSG00000032640; Mus musculus. [Genome view]
GeneID	269941.
KEGG	mmu:269941.
UCSC	uc009hhb.1. mouse.
Organism-specific databases
CTD	269941.
MGI	MGI:2681120. Chsy1.
Rouge	Search...
Phylogenomic databases
HOGENOM	HBG379060.
HOVERGEN	Q6ZQ11.
InParanoid	Q6ZQ11.
OMA	IIDFKEI.
OrthoDB	EOG934ZSF.
PhylomeDB	Q6ZQ11.
Enzyme and pathway databases
BRENDA	2.4.1.175. 244. 2.4.1.226. 244.
Gene expression databases
ArrayExpress	Q6ZQ11.
Bgee	Q6ZQ11.
CleanEx	MM_CHSY1.
Genevestigator	Q6ZQ11.
GermOnline	ENSMUSG00000032640. Mus musculus.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. [Graphical view]
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	393107.
SOURCE	Search...

Entry information

Entry name

CHSS1_MOUSE

Accession

Primary (citable) accession number: Q6ZQ11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)