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Protein

Chondroitin sulfate synthase 1

Gene

Chsy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Co2+By similarity, Mn2+By similarity, Cd2+By similarityNote: Divalent metal cations. Highest activities are measured with Co2+, Mn2+ and Cd2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi632 – 6321Divalent metal cationSequence analysis
Metal bindingi746 – 7461Divalent metal cationSequence analysis

GO - Molecular functioni

GO - Biological processi

  • bone morphogenesis Source: MGI
  • cartilage development Source: MGI
  • chondrocyte development Source: MGI
  • chondroitin sulfate biosynthetic process Source: MGI
  • negative regulation of ossification Source: MGI
  • positive regulation of smoothened signaling pathway Source: MGI
  • proximal/distal pattern formation Source: MGI
  • response to nutrient levels Source: Ensembl
  • sulfation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate synthase 1 (EC:2.4.1.175, EC:2.4.1.226)
Alternative name(s):
Chondroitin glucuronyltransferase 1
Chondroitin synthase 1
Short name:
ChSy-1
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1
N-acetylgalactosaminyltransferase 1
Gene namesi
Name:Chsy1
Synonyms:Kiaa0990
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2681120. Chsy1.

Subcellular locationi

  • Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
  • Secreted By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 800772LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 800800Chondroitin sulfate synthase 1PRO_0000189559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ6ZQ11.
MaxQBiQ6ZQ11.
PaxDbiQ6ZQ11.
PeptideAtlasiQ6ZQ11.
PRIDEiQ6ZQ11.

PTM databases

iPTMnetiQ6ZQ11.
PhosphoSiteiQ6ZQ11.

Expressioni

Gene expression databases

BgeeiQ6ZQ11.
CleanExiMM_CHSY1.
GenevisibleiQ6ZQ11. MM.

Interactioni

Subunit structurei

Binds CHPF.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047487.

Structurei

3D structure databases

ProteinModelPortaliQ6ZQ11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000220809.
HOVERGENiHBG050948.
InParanoidiQ6ZQ11.
KOiK13499.
OMAiYRIKYPK.
OrthoDBiEOG74TWXX.
PhylomeDBiQ6ZQ11.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

Q6ZQ11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARGRRAWL SMLLGLVLGF VLASRLVLPR ASELKRVGPR RRPSPEGCRP
60 70 80 90 100
GQEASQPGGA RGDARGAQLW PQGSAAEGVP RDRNFLFVGV MTAQKYLQTR
110 120 130 140 150
AVAAYRTWSK TIPGKVEFFS SEGSDTSIPI PVVPLRGVDD SYPPQKKSFM
160 170 180 190 200
MLKYMHDHYL DKYEWFMRAD DDVYIKGDRL ESFLRSLNSS EPLFLGQTGL
210 220 230 240 250
GTTEEMGKLA LEPGENFCMG GPGVILSREV LRRMAPHIGK CLREMYTTHE
260 270 280 290 300
DVEVGRCVRR FAGVQCVWSY EMQQLFYENY EQNKKGYIRD LHSSKIHRAI
310 320 330 340 350
TLHPNKNPPY QYRLHSYMLS RKIAELRHRT IQLHREIVLM SKYSSTEIQK
360 370 380 390 400
EDLQLGIPPS FMRFQARQRE EILEWEFLTG KYLYSATDGQ PPRRGMDSAQ
410 420 430 440 450
REALDDIVMQ VMEMINANAK TRGRIIDFKE IQYGYRRVNP MYGAEYILDL
460 470 480 490 500
LLLYKKHKGK KMTVPVRRHA YLQQTFSKMQ FVEHEELDAQ ELADRINQDS
510 520 530 540 550
GSLSFLSNSL KKLVAFQLPG SKTEHKEPKE KKINILIPLS GRFDMFVRFM
560 570 580 590 600
GNFEKTCLIP NLNVKLVILL FNSDSNPDKA KQVELMRDYR VKYPKADMQV
610 620 630 640 650
LPVSGGFSRA LALEVGSSQF NNESLLFFCD VDLVFTAEFL QRCRANTVLG
660 670 680 690 700
QQIYFPIIFS QYDPKIVYSG KVPSDNHFAF TQKTGFWRNY GFGITCIYKG
710 720 730 740 750
DLVRVGGFDV SIQGWGLEDV DLFNKVVQAG LKTFRSQEVG VVHIHHPVVC
760 770 780 790 800
DPNLDPKQYK MCLGSKASTF GSTQQLAEMW LEKNDPSYSK GGSHGSARTA
Length:800
Mass (Da):91,383
Last modified:February 1, 2005 - v2
Checksum:iF3DFBC6E7C78C21D
GO

Sequence cautioni

The sequence BAC98065.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129255 mRNA. Translation: BAC98065.1. Different initiation.
CCDSiCCDS39978.1.
RefSeqiNP_001074632.1. NM_001081163.1.
UniGeneiMm.333916.

Genome annotation databases

EnsembliENSMUST00000036372; ENSMUSP00000047487; ENSMUSG00000032640.
GeneIDi269941.
KEGGimmu:269941.
UCSCiuc009hhb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129255 mRNA. Translation: BAC98065.1. Different initiation.
CCDSiCCDS39978.1.
RefSeqiNP_001074632.1. NM_001081163.1.
UniGeneiMm.333916.

3D structure databases

ProteinModelPortaliQ6ZQ11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047487.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

PTM databases

iPTMnetiQ6ZQ11.
PhosphoSiteiQ6ZQ11.

Proteomic databases

EPDiQ6ZQ11.
MaxQBiQ6ZQ11.
PaxDbiQ6ZQ11.
PeptideAtlasiQ6ZQ11.
PRIDEiQ6ZQ11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036372; ENSMUSP00000047487; ENSMUSG00000032640.
GeneIDi269941.
KEGGimmu:269941.
UCSCiuc009hhb.1. mouse.

Organism-specific databases

CTDi22856.
MGIiMGI:2681120. Chsy1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000220809.
HOVERGENiHBG050948.
InParanoidiQ6ZQ11.
KOiK13499.
OMAiYRIKYPK.
OrthoDBiEOG74TWXX.
PhylomeDBiQ6ZQ11.
TreeFamiTF318303.

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiChsy1. mouse.
PROiQ6ZQ11.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZQ11.
CleanExiMM_CHSY1.
GenevisibleiQ6ZQ11. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic tail.

Entry informationi

Entry nameiCHSS1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQ11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: July 6, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.