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Protein

Protein SMG5

Gene

Smg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SMG5
Alternative name(s):
EST1-like protein B
SMG-5 homolog
Gene namesi
Name:Smg5
Synonyms:Est1b, Kiaa1089
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2447364. Smg5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Detected in cytoplasmic mRNA decay bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10171016Protein SMG5PRO_0000076323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei423 – 4231PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZPY2.
MaxQBiQ6ZPY2.
PaxDbiQ6ZPY2.
PRIDEiQ6ZPY2.

PTM databases

iPTMnetiQ6ZPY2.
PhosphoSiteiQ6ZPY2.

Expressioni

Gene expression databases

BgeeiQ6ZPY2.
ExpressionAtlasiQ6ZPY2. baseline and differential.
GenevisibleiQ6ZPY2. MM.

Interactioni

Subunit structurei

Interacts with TERT, PPP2CA and SMG1. Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001451.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPY2.
SMRiQ6ZPY2. Positions 856-1013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini873 – 996124PINcAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili798 – 84245Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi459 – 4635Poly-Arg

Sequence similaritiesi

Contains 1 PINc domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INQY. Eukaryota.
ENOG410XPF4. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000261635.
HOVERGENiHBG082268.
InParanoidiQ6ZPY2.
KOiK11125.
OMAiIIPFELS.
OrthoDBiEOG7GTT35.
PhylomeDBiQ6ZPY2.
TreeFamiTF327119.

Family and domain databases

InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 2 hits.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQGPPPGES SEPEAKVLHT KRLYRAVVEA VHRLDLILCN KAAYQEVFKP
60 70 80 90 100
ENVSLRNKLR ELCVKLMFLH PVDYGRKAEE LLWRKVYYEV IQLIKTNKKH
110 120 130 140 150
IHSRSTLECA YRTHLVAGIG FYQHLLLYIQ SHYQLELQCC IDWTHVTDPL
160 170 180 190 200
MGFKKPVSAS GKEMDWAQMA CHRCLVYLGD LSRYQNELAG VDTELLAERF
210 220 230 240 250
YYQALSVAPQ IGMPFNQLGT LAGSKYYNVE AMYCYLRCIQ SEVSFEGAYG
260 270 280 290 300
NLKRLYDKAA KMYHQLKKSE TRKLSPSKKR CKDIKRLLVN FMYLQSLLQP
310 320 330 340 350
KSSSVDSELT SLCQSVLEDF NLCLFYLPSS PNLGLTNEDE EECESGYAFL
360 370 380 390 400
PDLLIFQMAI ICLMGVHSLK RAGSKHYSAA IAFTLALFSH LINHVNIRLQ
410 420 430 440 450
AELEEGENPV SAFQSDGTDE PESKEALEKE EPEPEPPTVV PQADEGRKSR
460 470 480 490 500
KHSRLSCLRR RRRHHPPKAG DDSDLSEGFE SDSSHDSAQA SDGSDSGSDK
510 520 530 540 550
SLEGRGTAFD AETDSEMNSQ ESRSDLEDME DEEGTRSPAQ EPPQARSEVP
560 570 580 590 600
DSLNGPLGPS EASIASNLQA MSTQMFQTKR CFRLAPTFSN LLLQPTTEPN
610 620 630 640 650
SVASHRPCVN GDMDKPLEPA SEDGSESEGS ESSNRSCRNE RSLQEKLQAL
660 670 680 690 700
MAEGLLPAVK VFLDWLRTNP DLIIVCAQSS QSLWNRLSVL LNLLPASAEL
710 720 730 740 750
QDSGLALCSE VQGLLEGCEL PDLPASLLLP EDMALRNLPP LRAAHRRFNF
760 770 780 790 800
DADRPLLSAL EESVVRICCI RSFGHFVARL QGSILQFNPE VGIFVSIAQS
810 820 830 840 850
EQESLLQQAQ AQFRMAEEEA RRNRLMRDMA QLRLQLEVSQ LEGSLQQPKA
860 870 880 890 900
QSAMSPYLIP DTQALCYHLP LIRQLATSGR FIIIIPRTVI DGLDLLKKEQ
910 920 930 940 950
PGARDGIRYL EAEFKKGNRY IRCQKEVGKS FERHKLKRQD ADAWTLYKIL
960 970 980 990 1000
DSCRQLTLAQ GAGEEDPSGM VTIITGLHLD SPSALSGPMQ AALQAAAHAS
1010
VDVKNVLDFY RQWKEIG
Length:1,017
Mass (Da):114,071
Last modified:December 20, 2005 - v2
Checksum:i9925688D6FAFAF5C
GO
Isoform 2 (identifier: Q6ZPY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     620-702: ASEDGSESEG...LLPASAELQD → GAWVHESPPS...GSQKLGIMFL
     703-1017: Missing.

Show »
Length:702
Mass (Da):78,613
Checksum:iFD7AB5774BDF168D
GO

Sequence cautioni

The sequence BAC98096.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti77 – 771K → R in strain: B5/EGFP. 1 Publication
Natural varianti166 – 1661W → L in strain: Czech II. 1 Publication
Natural varianti499 – 4991D → E in strain: Czech II. 1 Publication
Natural varianti503 – 5031E → G in strain: Czech II. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei620 – 70283ASEDG…AELQD → GAWVHESPPSRRVTSPLGFC CHCGILVLCTYSFPASRTFA VLLLSTADCSSFTLFSPHIG GCTSFPHGIQLPEGSQKLGI MFL in isoform 2. 1 PublicationVSP_016590Add
BLAST
Alternative sequencei703 – 1017315Missing in isoform 2. 1 PublicationVSP_016591Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129286 mRNA. Translation: BAC98096.1. Different initiation.
BC024683 mRNA. Translation: AAH24683.3.
BC100408 mRNA. Translation: AAI00409.1.
CCDSiCCDS38480.1. [Q6ZPY2-1]
RefSeqiNP_839977.2. NM_178246.3. [Q6ZPY2-1]
UniGeneiMm.439830.

Genome annotation databases

EnsembliENSMUST00000001451; ENSMUSP00000001451; ENSMUSG00000001415. [Q6ZPY2-1]
GeneIDi229512.
KEGGimmu:229512.
UCSCiuc008pus.1. mouse. [Q6ZPY2-2]
uc008put.1. mouse. [Q6ZPY2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129286 mRNA. Translation: BAC98096.1. Different initiation.
BC024683 mRNA. Translation: AAH24683.3.
BC100408 mRNA. Translation: AAI00409.1.
CCDSiCCDS38480.1. [Q6ZPY2-1]
RefSeqiNP_839977.2. NM_178246.3. [Q6ZPY2-1]
UniGeneiMm.439830.

3D structure databases

ProteinModelPortaliQ6ZPY2.
SMRiQ6ZPY2. Positions 856-1013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001451.

PTM databases

iPTMnetiQ6ZPY2.
PhosphoSiteiQ6ZPY2.

Proteomic databases

EPDiQ6ZPY2.
MaxQBiQ6ZPY2.
PaxDbiQ6ZPY2.
PRIDEiQ6ZPY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001451; ENSMUSP00000001451; ENSMUSG00000001415. [Q6ZPY2-1]
GeneIDi229512.
KEGGimmu:229512.
UCSCiuc008pus.1. mouse. [Q6ZPY2-2]
uc008put.1. mouse. [Q6ZPY2-1]

Organism-specific databases

CTDi23381.
MGIiMGI:2447364. Smg5.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INQY. Eukaryota.
ENOG410XPF4. LUCA.
GeneTreeiENSGT00560000077120.
HOGENOMiHOG000261635.
HOVERGENiHBG082268.
InParanoidiQ6ZPY2.
KOiK11125.
OMAiIIPFELS.
OrthoDBiEOG7GTT35.
PhylomeDBiQ6ZPY2.
TreeFamiTF327119.

Enzyme and pathway databases

ReactomeiR-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiQ6ZPY2.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZPY2.
ExpressionAtlasiQ6ZPY2. baseline and differential.
GenevisibleiQ6ZPY2. MM.

Family and domain databases

InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 2 hits.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ARG-77; LEU-166; GLU-499 AND GLY-503.
    Strain: B5/EGFP, Czech II and ICR.
    Tissue: Mammary tumor and Trophoblast stem cell.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiSMG5_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPY2
Secondary accession number(s): Q497S2, Q8R3S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 8, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.