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Protein

DNA helicase INO80

Gene

Ino80

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double-stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi545 – 5528ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, Mitosis

Keywords - Ligandi

Actin-binding, ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase INO80 (EC:3.6.4.12)
Alternative name(s):
INO80 complex subunit A
Putative DNA helicase INO80 complex homolog 1
Gene namesi
Name:Ino80
Synonyms:Inoc1, Kiaa1259
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915392. Ino80.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Colocalizes with PCNA at replication forks during S-phase (By similarity). Recruited to DNA damage sites in a ACTR8-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15591559DNA helicase INO80PRO_0000248830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei118 – 1181N6-acetyllysineBy similarity
Modified residuei1515 – 15151PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZPV2.
MaxQBiQ6ZPV2.
PaxDbiQ6ZPV2.
PRIDEiQ6ZPV2.

PTM databases

iPTMnetiQ6ZPV2.
PhosphoSiteiQ6ZPV2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ6ZPV2.
ExpressionAtlasiQ6ZPV2. baseline and differential.
GenevisibleiQ6ZPV2. MM.

Interactioni

Subunit structurei

Component of the chromatin-remodeling INO80 complex; three different complex modules assemble on different domains of INO80. Interacts with DDB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212678. 1 interaction.
IntActiQ6ZPV2. 3 interactions.
STRINGi10090.ENSMUSP00000051845.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini282 – 407126DBINOPROSITE-ProRule annotationAdd
BLAST
Domaini532 – 703172Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1108 – 1263156Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Domaini

The DBINO region is involved in binding to DNA.By similarity

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 DBINO domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0388. Eukaryota.
ENOG410XP0A. LUCA.
GeneTreeiENSGT00740000115601.
HOGENOMiHOG000231795.
HOVERGENiHBG057875.
InParanoidiQ6ZPV2.
KOiK11665.
OMAiLQSESKC.
OrthoDBiEOG7B05C6.
PhylomeDBiQ6ZPV2.
TreeFamiTF324408.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020838. DBINO.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR031047. Ino80.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF665. PTHR10799:SF665. 9 hits.
PfamiPF13892. DBINO. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51413. DBINO. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPV2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASELGAGDD GSSTELAKPL YLQYLERALR LDHFLRQTSA IFNRNISSDD
60 70 80 90 100
SEDGLDDNNP LLPESGDPLI QVKEEPPNSL LGETSGASSS GLLNPYSLNG
110 120 130 140 150
VLQSESKSDK GNLYNFSKLK KSRKWLKSIL LSDESSEADS QSEDNDDEEE
160 170 180 190 200
ELSLSREELH NMLRLHKYKK LHQNKYSKDK ELQQYQYYSA GLLSTYDPFY
210 220 230 240 250
EQQRHLLGPK KKKFKEDKKL KAKLKKVKKK RRRDEEFSSE ESPRHHHHQT
260 270 280 290 300
KVFAKFSHDA PPPGTKKKHL SIEQLNARRR KVWLSIVKKE LPKANKQKSS
310 320 330 340 350
ARNLFLTNSR KLAHQCMKEV RRAALQAQKN CKETLPRARR LTKEMLLYWK
360 370 380 390 400
KYEKVEKEHR KRAEKEALEQ RKLDEEMREA KRQQRKLNFL ITQTELYAHF
410 420 430 440 450
MSRKRDMGHD GIQEEILRKL EDSSTQRQID IGGGVVVNIT QEDYDSNHFK
460 470 480 490 500
AQALKNAENA YHIHQARTRS FDEDAKESRA AALRAADKSG SGFGESYSLA
510 520 530 540 550
NPSIRAGEDI PQPTIFNGKL KGYQLKGMNW LANLYEQGIN GILADEMGLG
560 570 580 590 600
KTVQSIALLA HLAERENIWG PFLIISPAST LNNWHQEFTR FVPKFKVLPY
610 620 630 640 650
WGNPHDRKVI RRFWSQKTLY TQDAPFHVVI TSYQLVVQDV KYFQRVKWQY
660 670 680 690 700
MVLDEAQALK SSSSVRWKIL LQFQCRNRLL LTGTPIQNTM AELWALLHFI
710 720 730 740 750
MPTLFDSHEE FNEWFSKDIE SHAENKSAID ENQLSRLHMI LKPFMLRRIK
760 770 780 790 800
KDVENELSDK IEILTYCQLT SRQKLLYQAL KNKISIEDLL QSSMGSTQQA
810 820 830 840 850
QNTTSSLMNL VMQFRKVCNH PELFERQETW SPFHISLKPY EISKFIYRHG
860 870 880 890 900
QIRVFNHSRD RWLKVLLSPF APDYIQQSLF HRKGINEGSC FSFLRFIDVS
910 920 930 940 950
PAEMANLMLQ GLLARWLALF LSLKASYRLH QLRSWAEPDG TSHQSYLRNK
960 970 980 990 1000
DFLLGVDFPL SFPNLCSCPL LKSLVFSSHC KAVSGYSDHV VHQRRSATSS
1010 1020 1030 1040 1050
LRCCLLTELP SFLCVASPRV TAVPLDSYCN DRSAEYERGV LKEGGSLAAK
1060 1070 1080 1090 1100
QCLLNGAPEL ATDWLSRRSQ FFPEPAGGLL SIRPQNGWSF IRIPGKESLI
1110 1120 1130 1140 1150
TDSGKLYALD VLLTRLKSQG HRVLIYSQMT RMIDLLEEYM VYRKHTYMRL
1160 1170 1180 1190 1200
DGSSKISERR DMVADFQTRN DIFVFLLSTR AGGLGINLTA ADTVIFYDSD
1210 1220 1230 1240 1250
WNPTVDQQAM DRAHRLGQTK QVTVYRLICK GTIEERILQR AKEKSEIQRM
1260 1270 1280 1290 1300
VISGGNFKPD TLKPKEVVSL LLDDEELEKK LRLRQEEKRQ QEESNRVKER
1310 1320 1330 1340 1350
KRKREKYAEK KKKEDELDGK RRKEGVNLVI PFVPSADNSN LSADGDDSFI
1360 1370 1380 1390 1400
SVDSAMPSPF SEISISSELH TGSIPPDESS SDMLVIVDDP ASSAPQSRAT
1410 1420 1430 1440 1450
NSPASITGSV SDTVNGISIQ EVPAAGRGHS ARSRGRPKGS GSTAKGAGKG
1460 1470 1480 1490 1500
RSRKSTAGSA AAMAGAKAGA AAASAAAYAA YGYNVSKGIS ASSPLQTSIV
1510 1520 1530 1540 1550
RPAGLADFGP SSASSPLSSP LNKGNNIPGT PKSLHMTSSL ASDSLIRKQG

KGTNPSGGR
Length:1,559
Mass (Da):176,520
Last modified:September 5, 2006 - v2
Checksum:iA02B780D0B13D08F
GO
Isoform 2 (identifier: Q6ZPV2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     608-614: Missing.

Show »
Length:1,552
Mass (Da):175,533
Checksum:iC502A210B56F55AD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei608 – 6147Missing in isoform 2. 1 PublicationVSP_020334

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844862 Genomic DNA. Translation: CAM22021.1.
AK040612 mRNA. Translation: BAC30644.1.
AK129317 Transcribed RNA. Translation: BAC98127.1.
BC059235 mRNA. Translation: AAH59235.1.
CCDSiCCDS16602.1. [Q6ZPV2-1]
RefSeqiNP_080850.2. NM_026574.3. [Q6ZPV2-1]
UniGeneiMm.330496.

Genome annotation databases

EnsembliENSMUST00000049920; ENSMUSP00000051845; ENSMUSG00000034154. [Q6ZPV2-1]
GeneIDi68142.
KEGGimmu:68142.
UCSCiuc008ltt.2. mouse. [Q6ZPV2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844862 Genomic DNA. Translation: CAM22021.1.
AK040612 mRNA. Translation: BAC30644.1.
AK129317 Transcribed RNA. Translation: BAC98127.1.
BC059235 mRNA. Translation: AAH59235.1.
CCDSiCCDS16602.1. [Q6ZPV2-1]
RefSeqiNP_080850.2. NM_026574.3. [Q6ZPV2-1]
UniGeneiMm.330496.

3D structure databases

ProteinModelPortaliQ6ZPV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212678. 1 interaction.
IntActiQ6ZPV2. 3 interactions.
STRINGi10090.ENSMUSP00000051845.

PTM databases

iPTMnetiQ6ZPV2.
PhosphoSiteiQ6ZPV2.

Proteomic databases

EPDiQ6ZPV2.
MaxQBiQ6ZPV2.
PaxDbiQ6ZPV2.
PRIDEiQ6ZPV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049920; ENSMUSP00000051845; ENSMUSG00000034154. [Q6ZPV2-1]
GeneIDi68142.
KEGGimmu:68142.
UCSCiuc008ltt.2. mouse. [Q6ZPV2-1]

Organism-specific databases

CTDi54617.
MGIiMGI:1915392. Ino80.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0388. Eukaryota.
ENOG410XP0A. LUCA.
GeneTreeiENSGT00740000115601.
HOGENOMiHOG000231795.
HOVERGENiHBG057875.
InParanoidiQ6ZPV2.
KOiK11665.
OMAiLQSESKC.
OrthoDBiEOG7B05C6.
PhylomeDBiQ6ZPV2.
TreeFamiTF324408.

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.

Miscellaneous databases

PROiQ6ZPV2.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZPV2.
ExpressionAtlasiQ6ZPV2. baseline and differential.
GenevisibleiQ6ZPV2. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR020838. DBINO.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR031047. Ino80.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF665. PTHR10799:SF665. 9 hits.
PfamiPF13892. DBINO. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51413. DBINO. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-746 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 357-1559 (ISOFORM 2).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 472-1559 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity."
    Bakshi R., Mehta A.K., Sharma R., Maiti S., Pasha S., Brahmachari V.
    Biochem. Biophys. Res. Commun. 339:313-320(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "The mammalian INO80 complex is recruited to DNA damage sites in an ARP8 dependent manner."
    Kashiwaba S., Kitahashi K., Watanabe T., Onoda F., Ohtsu M., Murakami Y.
    Biochem. Biophys. Res. Commun. 402:619-625(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiINO80_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPV2
Secondary accession number(s): A2AQP8
, Q6P7V0, Q6PCP1, Q8C9T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.