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Protein

Ankyrin repeat and IBR domain-containing protein 1

Gene

Ankib1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei533By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri334 – 384RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri403 – 479IBR-type 1; degenerateAdd BLAST77
Zinc fingeri520 – 549RING-type 2PROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and IBR domain-containing protein 1 (EC:2.3.2.-By similarity)
Gene namesi
Name:Ankib1
Synonyms:Kiaa1386
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1918047. Ankib1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000668932 – 1085Ankyrin repeat and IBR domain-containing protein 1Add BLAST1084

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei738PhosphoserineCombined sources1
Modified residuei879PhosphoserineBy similarity1
Modified residuei906PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ6ZPS6.
MaxQBiQ6ZPS6.
PaxDbiQ6ZPS6.
PRIDEiQ6ZPS6.

PTM databases

iPTMnetiQ6ZPS6.
PhosphoSitePlusiQ6ZPS6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040351.
CleanExiMM_ANKIB1.
ExpressionAtlasiQ6ZPS6. baseline and differential.
GenevisibleiQ6ZPS6. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040946.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 75ANK 1Add BLAST31
Repeati145 – 174ANK 2Add BLAST30
Domaini846 – 865UIMPROSITE-ProRule annotationAdd BLAST20

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili576 – 641Sequence analysisAdd BLAST66

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family.Curated
Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri334 – 384RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri403 – 479IBR-type 1; degenerateAdd BLAST77
Zinc fingeri520 – 549RING-type 2PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOVERGENiHBG083226.
InParanoidiQ6ZPS6.
KOiK11967.
OMAiNNPAIKW.
OrthoDBiEOG091G00NA.
PhylomeDBiQ6ZPS6.
TreeFamiTF331104.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR003903. UIM_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF00023. Ank. 2 hits.
PF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ZPS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNTTTKFRK ALINGDENLA CQIYENNPQL KESLDPNISY GEPYQHNTPL
60 70 80 90 100
HYAARHGMNR ILGTFLFGRD GNPNKRNVHN ETSMHLLCMG PQIMISEGTL
110 120 130 140 150
HPRLARPVED DFRRADCLQM ILQWKGAKLD QGEYERAAID AVDNKKNTPL
160 170 180 190 200
HYAAASGMKA CVELLVKHGG DLFAENENRD TPCDCAEKQQ HKDLALSLES
210 220 230 240 250
QMVFSRDPEA EEIEAEYAAL DKREPYEGLR PQDLRRLKDM LIVETADMLQ
260 270 280 290 300
APLFTAEALL RAHDWDREKL LEAWMSNPEN CCQRSGVQMP TPPPSGYNAW
310 320 330 340 350
DTLPSPRTPR TTRSSVTSPD EISLSPGDLD TSLCDICMCS ISVFEDPVDM
360 370 380 390 400
PCGHDFCRGC WEAFLNLKIQ EGEAHNIFCP AYECFQLVPV DVIESVVSKE
410 420 430 440 450
MDKRYLQFDI KAFVENNPAI KWCPTAGCER AVRLTKQGSN PSGSDTLSFP
460 470 480 490 500
LLRAPAVDCG KGHLFCWECL GEAHEPCDCQ TWKNWLQKIT EMKPEELVGV
510 520 530 540 550
SEAYEDAANC LWLLTNSKPC ANCKSPIQKN EGCNHMQCAK CKYDFCWICL
560 570 580 590 600
EEWKKHSSST GGYYRCTRYE VIQHVEEQSK EMTVEAEKKH KRFQELDRFM
610 620 630 640 650
HYYTRYKNHE HSYQLEQRLL KTAKEKMEQL SRALKETEGG CPDTTFIEDA
660 670 680 690 700
VHVLLKTRRI LKCSYPYGFF LEPKSTKKEI FELMQTDLEM VTEDLAQKVN
710 720 730 740 750
RPYLRTPRHK IIRAACLVQQ KRQEFLASVA RGVAPADSPD APRRSFAGGT
760 770 780 790 800
WDWEYLGFAS PEYRRRHRQQ RRRGDVHSLL SNPTDLDEPS ESTFDLPEGS
810 820 830 840 850
SGRRPGASVV SSASMSVLHS SSLRDYSPAS RSANQDSLQA LSSLDEDDPN
860 870 880 890 900
ILLAIQLSLQ ESGLDMDEET RDFLSNETSL GAIGSSLPSR LDSVPRSTES
910 920 930 940 950
PRAALSSSEL LELGDSLMRL GADSDPFSTD TLSSRPLSET RSDFCPSSSD
960 970 980 990 1000
LDSAGQDPSA NDNLLGNIMA WFHDMNPQSI ALIPPAATTE ISAEPQLPCI
1010 1020 1030 1040 1050
RDGSEGVRDM ELVPPEDSVS KDTGVHEGER AQMEENPLEE NILAREELSQ
1060 1070 1080
AGDSSNEAVG RGDRPDAASQ TPQTSSDWLE QVHSV
Length:1,085
Mass (Da):121,876
Last modified:July 24, 2007 - v2
Checksum:iEAB5F58A624F3972
GO

Sequence cautioni

The sequence AAH79620 differs from that shown. Reason: Erroneous termination at position 616. Translated as Glu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129343 mRNA. Translation: BAC98153.1.
BC079620 mRNA. Translation: AAH79620.1. Sequence problems.
CCDSiCCDS51408.1.
RefSeqiNP_001003909.2. NM_001003909.4.
NP_001276457.1. NM_001289528.1.
NP_001276458.1. NM_001289529.1.
UniGeneiMm.130148.

Genome annotation databases

EnsembliENSMUST00000043551; ENSMUSP00000040946; ENSMUSG00000040351.
GeneIDi70797.
KEGGimmu:70797.
UCSCiuc008who.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129343 mRNA. Translation: BAC98153.1.
BC079620 mRNA. Translation: AAH79620.1. Sequence problems.
CCDSiCCDS51408.1.
RefSeqiNP_001003909.2. NM_001003909.4.
NP_001276457.1. NM_001289528.1.
NP_001276458.1. NM_001289529.1.
UniGeneiMm.130148.

3D structure databases

ProteinModelPortaliQ6ZPS6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040946.

PTM databases

iPTMnetiQ6ZPS6.
PhosphoSitePlusiQ6ZPS6.

Proteomic databases

EPDiQ6ZPS6.
MaxQBiQ6ZPS6.
PaxDbiQ6ZPS6.
PRIDEiQ6ZPS6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043551; ENSMUSP00000040946; ENSMUSG00000040351.
GeneIDi70797.
KEGGimmu:70797.
UCSCiuc008who.3. mouse.

Organism-specific databases

CTDi54467.
MGIiMGI:1918047. Ankib1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOVERGENiHBG083226.
InParanoidiQ6ZPS6.
KOiK11967.
OMAiNNPAIKW.
OrthoDBiEOG091G00NA.
PhylomeDBiQ6ZPS6.
TreeFamiTF331104.

Miscellaneous databases

PROiQ6ZPS6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040351.
CleanExiMM_ANKIB1.
ExpressionAtlasiQ6ZPS6. baseline and differential.
GenevisibleiQ6ZPS6. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR003903. UIM_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF00023. Ank. 2 hits.
PF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKIB1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPS6
Secondary accession number(s): Q6AXE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 24, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Lacks one Cys residue in the IBR-type zinc finger domain that is one of the conserved features of the family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.