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Protein

Carnosine synthase 1

Gene

Carns1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of carnosine and homocarnosine. Carnosine is synthesized more efficiently than homocarnosine.1 Publication

Catalytic activityi

ATP + L-histidine + beta-alanine = ADP + phosphate + carnosine.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Kineticsi

  1. KM=0.46 mM for beta-alanine1 Publication
  2. KM=6.44 mM for gamma-aminobutyrate1 Publication
  3. KM=0.11 mM for L-histidine1 Publication
  4. KM=1.59 mM for L-lysine1 Publication
  5. KM=0.52 mM for L-ornithine1 Publication
  6. KM=4.51 mM for N-methylhistidine1 Publication
  1. Vmax=300 nmol/min/mg enzyme toward beta-alanine1 Publication
  2. Vmax=238 nmol/min/mg enzyme toward gamma-aminobutyrate1 Publication
  3. Vmax=0.60 nmol/min/mg enzyme toward L-histidine1 Publication
  4. Vmax=0.78 nmol/min/mg enzyme toward L-lysine1 Publication
  5. Vmax=1.92 nmol/min/mg enzyme toward L-ornithine1 Publication
  6. Vmax=0.66 nmol/min/mg enzyme toward N-methylhistidine1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi677Manganese 1By similarity1
Metal bindingi689Manganese 1By similarity1
Metal bindingi689Manganese 2By similarity1
Metal bindingi691Manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi542 – 611ATPAdd BLAST70

GO - Molecular functioni

  • ATPase activity Source: MGI
  • ATP binding Source: UniProtKB-KW
  • carnosine synthase activity Source: UniProtKB
  • homocarnosine synthase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carnosine biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionLigase
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.11. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Carnosine synthase 1 (EC:6.3.2.11)
Alternative name(s):
ATP-grasp domain-containing protein 1
Gene namesi
Name:Carns1
Synonyms:Atpgd1, Kiaa1394
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2147595. Carns1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003290371 – 827Carnosine synthase 1Add BLAST827

Proteomic databases

PaxDbiQ6ZPS2.
PRIDEiQ6ZPS2.

PTM databases

iPTMnetiQ6ZPS2.
PhosphoSitePlusiQ6ZPS2.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131624.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPS2.
SMRiQ6ZPS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini516 – 720ATP-graspAdd BLAST205

Phylogenomic databases

eggNOGiENOG410IH96. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000171794.
HOVERGENiHBG102303.
InParanoidiQ6ZPS2.
PhylomeDBiQ6ZPS2.

Family and domain databases

InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR031046. CARNS1.
PANTHERiPTHR10578:SF57. PTHR10578:SF57. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLCLSPAWL MKVATPGQEG EAVLLVSKAV SFYPGGLTFL DDFVPPRHAT
60 70 80 90 100
YFLAGLGPES GRGKEAAELA RNLTCPTGTS SELSQLLENR LLMRWLLSQQ
110 120 130 140 150
SGVAVPATLA FTYRPPGLLR GGDASPGLRL VELSGKEGQE TLVKEEVEAF
160 170 180 190 200
VHSEALGDAS QVAVRLSGCR WRGQQALPLH LRVEPSTVVN TVLGLLEKLE
210 220 230 240 250
EEESVLVEAM CPPVRLPLPG GPAPGPELAV RICAVVCRIQ GDRPLLSKVV
260 270 280 290 300
CGVGRGDRPV RHHYTLPRTL RVALAQCGLE EEAQVALLEQ GIKEAAEGAL
310 320 330 340 350
AAVLALEAGL SVEQRGGRQV HTDFLGVDLV LTVIGRTLTP VVLKLNSGLC
360 370 380 390 400
LEACGALEGL WAVPRLRRSA EEAAAAPLVE TMLRRSGRHL MDGKQLLVIG
410 420 430 440 450
AGGVSKKFVW EAARDYGLTL HLVESDPNHF ASQLVQTFIH FDVTEHRRDE
460 470 480 490 500
ENALLLAELV RARNLKLDGC FSFWDDCLVL TALLCRELGL PCSPPAAMCL
510 520 530 540 550
AKQKSRTQLH LLRCQGPPWP STSLHAVACC PLENEADVER AIYQVPLPGV
560 570 580 590 600
MKLEFGSGAV GVQLVKDGPQ CREHFSRILH DLQGEADHPG IGLGWGNAML
610 620 630 640 650
LMEFVEGTEH DVDLVVFGGR LLAAFVSDNG PTRLPGFTET AACMPTGLAP
660 670 680 690 700
EQEAQVVQAA FRCCLGCGLL DGVFNVELKM TGAGPRLIEI NPRMGGFYLR
710 720 730 740 750
DWILELYGVD LLLASTMVAC GLQPALPAHP RARGYLVGIM CLVSQHLQLL
760 770 780 790 800
SSPSSRETLQ TLHDQGQLRL NLLEEALIPG QYEEPYCNVA CAGPSPAEAC
810 820
HRLLGICQGL GIDRPNYPVA HFLSHFK
Length:827
Mass (Da):89,281
Last modified:April 8, 2008 - v2
Checksum:i5AB9CAEB92994FE9
GO
Isoform 2 (identifier: Q6ZPS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLCLDPLGTE...EGGVQSPGNM
     163-267: Missing.

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):90,769
Checksum:i8BDD47DC535F8653
GO
Isoform 3 (identifier: Q6ZPS2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-497: Missing.

Show »
Length:330
Mass (Da):35,783
Checksum:i1FDA53D48E74FC8A
GO

Sequence cautioni

The sequence BAC98157 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti656V → M in AAH19736 (PubMed:15489334).Curated1
Sequence conflicti656V → M in AAH23699 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0329271 – 497Missing in isoform 3. 1 PublicationAdd BLAST497
Alternative sequenceiVSP_0329281M → MLCLDPLGTEWDSKDLDGKE EPWKSGAGLPPTGCFPGPWR QDISLDCKGSPEETEARAWT VYYYGLLQSCLQQAGLPETQ DRSQAPRTGCPGAEVTLCIL GSPSTFLSLLLEGGVQSPGN M in isoform 2. 1 Publication1
Alternative sequenceiVSP_032929163 – 267Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129347 Transcribed RNA. Translation: BAC98157.1. Different initiation.
AC109138 Genomic DNA. No translation available.
BC019736 mRNA. Translation: AAH19736.1.
BC023699 mRNA. Translation: AAH23699.1.
RefSeqiXP_006531682.1. XM_006531619.2. [Q6ZPS2-1]
XP_017173530.1. XM_017318041.1. [Q6ZPS2-1]
UniGeneiMm.482310.

Genome annotation databases

GeneIDi107239.
UCSCiuc012bge.1. mouse. [Q6ZPS2-3]
uc029tqn.1. mouse. [Q6ZPS2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCRNS1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPS2
Secondary accession number(s): Q8CIK7, Q8VE56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: October 25, 2017
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot