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Protein

Carnosine synthase 1

Gene

Carns1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of carnosine and homocarnosine. Carnosine is synthesized more efficiently than homocarnosine.1 Publication

Catalytic activityi

ATP + L-histidine + beta-alanine = ADP + phosphate + carnosine.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Kineticsi

  1. KM=0.46 mM for beta-alanine1 Publication
  2. KM=6.44 mM for gamma-aminobutyrate1 Publication
  3. KM=0.11 mM for L-histidine1 Publication
  4. KM=1.59 mM for L-lysine1 Publication
  5. KM=0.52 mM for L-ornithine1 Publication
  6. KM=4.51 mM for N-methylhistidine1 Publication
  1. Vmax=300 nmol/min/mg enzyme toward beta-alanine1 Publication
  2. Vmax=238 nmol/min/mg enzyme toward gamma-aminobutyrate1 Publication
  3. Vmax=0.60 nmol/min/mg enzyme toward L-histidine1 Publication
  4. Vmax=0.78 nmol/min/mg enzyme toward L-lysine1 Publication
  5. Vmax=1.92 nmol/min/mg enzyme toward L-ornithine1 Publication
  6. Vmax=0.66 nmol/min/mg enzyme toward N-methylhistidine1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi677 – 6771Manganese 1By similarity
Metal bindingi689 – 6891Manganese 1By similarity
Metal bindingi689 – 6891Manganese 2By similarity
Metal bindingi691 – 6911Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi542 – 61170ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATPase activity Source: MGI
  • ATP binding Source: UniProtKB-KW
  • carnosine synthase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carnosine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.11. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Carnosine synthase 1 (EC:6.3.2.11)
Alternative name(s):
ATP-grasp domain-containing protein 1
Gene namesi
Name:Carns1
Synonyms:Atpgd1, Kiaa1394
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2147595. Carns1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 827827Carnosine synthase 1PRO_0000329037Add
BLAST

Proteomic databases

MaxQBiQ6ZPS2.
PaxDbiQ6ZPS2.
PRIDEiQ6ZPS2.

PTM databases

iPTMnetiQ6ZPS2.
PhosphoSiteiQ6ZPS2.

Expressioni

Gene expression databases

BgeeiQ6ZPS2.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131624.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPS2.
SMRiQ6ZPS2. Positions 537-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini516 – 720205ATP-graspPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH96. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000171794.
HOVERGENiHBG102303.
InParanoidiQ6ZPS2.
PhylomeDBiQ6ZPS2.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR031046. CARNS1.
[Graphical view]
PANTHERiPTHR10578:SF57. PTHR10578:SF57. 2 hits.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLCLSPAWL MKVATPGQEG EAVLLVSKAV SFYPGGLTFL DDFVPPRHAT
60 70 80 90 100
YFLAGLGPES GRGKEAAELA RNLTCPTGTS SELSQLLENR LLMRWLLSQQ
110 120 130 140 150
SGVAVPATLA FTYRPPGLLR GGDASPGLRL VELSGKEGQE TLVKEEVEAF
160 170 180 190 200
VHSEALGDAS QVAVRLSGCR WRGQQALPLH LRVEPSTVVN TVLGLLEKLE
210 220 230 240 250
EEESVLVEAM CPPVRLPLPG GPAPGPELAV RICAVVCRIQ GDRPLLSKVV
260 270 280 290 300
CGVGRGDRPV RHHYTLPRTL RVALAQCGLE EEAQVALLEQ GIKEAAEGAL
310 320 330 340 350
AAVLALEAGL SVEQRGGRQV HTDFLGVDLV LTVIGRTLTP VVLKLNSGLC
360 370 380 390 400
LEACGALEGL WAVPRLRRSA EEAAAAPLVE TMLRRSGRHL MDGKQLLVIG
410 420 430 440 450
AGGVSKKFVW EAARDYGLTL HLVESDPNHF ASQLVQTFIH FDVTEHRRDE
460 470 480 490 500
ENALLLAELV RARNLKLDGC FSFWDDCLVL TALLCRELGL PCSPPAAMCL
510 520 530 540 550
AKQKSRTQLH LLRCQGPPWP STSLHAVACC PLENEADVER AIYQVPLPGV
560 570 580 590 600
MKLEFGSGAV GVQLVKDGPQ CREHFSRILH DLQGEADHPG IGLGWGNAML
610 620 630 640 650
LMEFVEGTEH DVDLVVFGGR LLAAFVSDNG PTRLPGFTET AACMPTGLAP
660 670 680 690 700
EQEAQVVQAA FRCCLGCGLL DGVFNVELKM TGAGPRLIEI NPRMGGFYLR
710 720 730 740 750
DWILELYGVD LLLASTMVAC GLQPALPAHP RARGYLVGIM CLVSQHLQLL
760 770 780 790 800
SSPSSRETLQ TLHDQGQLRL NLLEEALIPG QYEEPYCNVA CAGPSPAEAC
810 820
HRLLGICQGL GIDRPNYPVA HFLSHFK
Length:827
Mass (Da):89,281
Last modified:April 8, 2008 - v2
Checksum:i5AB9CAEB92994FE9
GO
Isoform 2 (identifier: Q6ZPS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLCLDPLGTE...EGGVQSPGNM
     163-267: Missing.

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):90,769
Checksum:i8BDD47DC535F8653
GO
Isoform 3 (identifier: Q6ZPS2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-497: Missing.

Show »
Length:330
Mass (Da):35,783
Checksum:i1FDA53D48E74FC8A
GO

Sequence cautioni

The sequence BAC98157.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti656 – 6561V → M in AAH19736 (PubMed:15489334).Curated
Sequence conflicti656 – 6561V → M in AAH23699 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 497497Missing in isoform 3. 1 PublicationVSP_032927Add
BLAST
Alternative sequencei1 – 11M → MLCLDPLGTEWDSKDLDGKE EPWKSGAGLPPTGCFPGPWR QDISLDCKGSPEETEARAWT VYYYGLLQSCLQQAGLPETQ DRSQAPRTGCPGAEVTLCIL GSPSTFLSLLLEGGVQSPGN M in isoform 2. 1 PublicationVSP_032928
Alternative sequencei163 – 267105Missing in isoform 2. 1 PublicationVSP_032929Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129347 Transcribed RNA. Translation: BAC98157.1. Different initiation.
AC109138 Genomic DNA. No translation available.
BC019736 mRNA. Translation: AAH19736.1.
BC023699 mRNA. Translation: AAH23699.1.
RefSeqiXP_006531682.1. XM_006531619.1. [Q6ZPS2-1]
XP_006544945.1. XM_006544882.1. [Q6ZPS2-1]
UniGeneiMm.482310.

Genome annotation databases

GeneIDi107239.
UCSCiuc012bge.1. mouse. [Q6ZPS2-3]
uc029tqn.1. mouse. [Q6ZPS2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129347 Transcribed RNA. Translation: BAC98157.1. Different initiation.
AC109138 Genomic DNA. No translation available.
BC019736 mRNA. Translation: AAH19736.1.
BC023699 mRNA. Translation: AAH23699.1.
RefSeqiXP_006531682.1. XM_006531619.1. [Q6ZPS2-1]
XP_006544945.1. XM_006544882.1. [Q6ZPS2-1]
UniGeneiMm.482310.

3D structure databases

ProteinModelPortaliQ6ZPS2.
SMRiQ6ZPS2. Positions 537-711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131624.

PTM databases

iPTMnetiQ6ZPS2.
PhosphoSiteiQ6ZPS2.

Proteomic databases

MaxQBiQ6ZPS2.
PaxDbiQ6ZPS2.
PRIDEiQ6ZPS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107239.
UCSCiuc012bge.1. mouse. [Q6ZPS2-3]
uc029tqn.1. mouse. [Q6ZPS2-1]

Organism-specific databases

CTDi57571.
MGIiMGI:2147595. Carns1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IH96. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000171794.
HOVERGENiHBG102303.
InParanoidiQ6ZPS2.
PhylomeDBiQ6ZPS2.

Enzyme and pathway databases

BRENDAi6.3.2.11. 3474.

Miscellaneous databases

PROiQ6ZPS2.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZPS2.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR031046. CARNS1.
[Graphical view]
PANTHERiPTHR10578:SF57. PTHR10578:SF57. 2 hits.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic tail.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  4. "Molecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1)."
    Drozak J., Veiga-da-Cunha M., Vertommen D., Stroobant V., Van Schaftingen E.
    J. Biol. Chem. 285:9346-9356(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiCRNS1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPS2
Secondary accession number(s): Q8CIK7, Q8VE56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: January 20, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.