Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inhibitor of Bruton tyrosine kinase

Gene

Ibtk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of Bruton tyrosine kinase
Short name:
IBtk
Gene namesi
Name:Ibtk
Synonyms:Kiaa1417
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918677. Ibtk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13521352Inhibitor of Bruton tyrosine kinasePRO_0000280277Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei991 – 9911PhosphoserineBy similarity
Modified residuei1005 – 10051PhosphoserineCombined sources
Modified residuei1031 – 10311PhosphoserineBy similarity
Modified residuei1034 – 10341PhosphoserineBy similarity
Modified residuei1040 – 10401PhosphoserineBy similarity
Modified residuei1046 – 10461PhosphoserineCombined sources
Modified residuei1055 – 10551PhosphoserineBy similarity
Modified residuei1111 – 11111PhosphoserineCombined sources
Modified residuei1113 – 11131PhosphoserineCombined sources
Modified residuei1116 – 11161PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZPR6.
MaxQBiQ6ZPR6.
PaxDbiQ6ZPR6.
PRIDEiQ6ZPR6.

PTM databases

iPTMnetiQ6ZPR6.
PhosphoSiteiQ6ZPR6.

Expressioni

Gene expression databases

BgeeiQ6ZPR6.
ExpressionAtlasiQ6ZPR6. baseline and differential.
GenevisibleiQ6ZPR6. MM.

Interactioni

Subunit structurei

Interacts with the PH domain of BTK.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224442. 1 interaction.
STRINGi10090.ENSMUSP00000041145.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPR6.
SMRiQ6ZPR6. Positions 48-422, 776-900.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 8030ANK 1Add
BLAST
Repeati85 – 11430ANK 2Add
BLAST
Repeati141 – 19454RCC1 1Add
BLAST
Repeati195 – 24652RCC1 2Add
BLAST
Repeati248 – 30154RCC1 3Add
BLAST
Domaini565 – 64581BTB 1PROSITE-ProRule annotationAdd
BLAST
Domaini769 – 83769BTB 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 2 BTB (POZ) domains.PROSITE-ProRule annotation
Contains 3 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0783. Eukaryota.
COG5184. LUCA.
GeneTreeiENSGT00830000128260.
HOGENOMiHOG000090207.
HOVERGENiHBG081779.
InParanoidiQ6ZPR6.
OMAiVSALHHK.
OrthoDBiEOG7K6PT6.
TreeFamiTF323747.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.130.10.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 2 hits.
PF00415. RCC1. 3 hits.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50985. SSF50985. 1 hit.
SSF54695. SSF54695. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50097. BTB. 2 hits.
PS50012. RCC1_3. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPR6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAATPDCTS KCRSLKHALD VLSVVTKGSE SQIKSFLARY CYNAATVKDA
60 70 80 90 100
FGRNAGHLAS SCGKKGVLDW LIEKGVDLLV KDKESGWTAL HRSVFYGHID
110 120 130 140 150
CVWSLLKHGV SLYMQDKEGL SPLDLLMKDR PTHVVFKDTD PTEVYTWGDN
160 170 180 190 200
TNFTLGHGSQ NSKHHPELLD LFSRSGVYVK QVVLCKFHSV FLSQKGQVYT
210 220 230 240 250
CGHGRGGRLG HGDEQTCLVP RLVEGLSGHN CSQVAAAKDH TVVLTDDGCV
260 270 280 290 300
YTFGLNMFHQ LGIIPPPASC NVPRQIQAKY LKGRTIIGVA AGRFHTVLWT
310 320 330 340 350
REAVYTLGLN GGQLGHLLDP NGEKCVTTPR QVSALHHKDI AVSLVAASDG
360 370 380 390 400
ATVCVTTRGD IYLLADYQCK KMATKQLNLK KVLVSGGCME YKVDPEHLTE
410 420 430 440 450
NGGQKICVLA MDGAGRVFCW RSISSSLKQC RWAYPRQVSI SDIALNRNEI
460 470 480 490 500
LFVTQDGEGF KGKWFEDKRK NSEKKADILP NLHHSSSDVS CVPDTNSVYE
510 520 530 540 550
RIRLEKLPFA HRAVSVSTDP SGCNFAILQS DPKTSLYEIP VVSSSSFFEE
560 570 580 590 600
FGKLLRETDE MDSFHDVTFQ VGNRHFPAHK YILAVRSDFF QKLFLSDGSS
610 620 630 640 650
LELTDVYQKD EDAAGCHLFV VEKVHPDLFE YLLQFMYTDT CDLLTHGFKP
660 670 680 690 700
RMIVKRKAED CEGSPDSHLH TVNCHVDDKQ KSAFEVYRSN QAHTLSERQK
710 720 730 740 750
SKPKSSKKGK GVGDDDPVRM LQSVAKKFGL SNLSSRLEGV RLENEKINVI
760 770 780 790 800
AKKTGNKLKL SQKKCSFLYD VTMKSVDGKE FSCHKCVLCA RLEYFHSMLS
810 820 830 840 850
RSWIEASSCA ALEMPIQSEI LKVILDYLYT DEAVVIKESQ NVDFVCSVLV
860 870 880 890 900
VADQLLITRL KEICEVALTE NLTLKNAAML LEFAALYNAG QLKLSCLQFI
910 920 930 940 950
GLNMAALLEA RSLDVLSEDV LKDLSIFYRK MIPAMERRVI TPYQDGPDIS
960 970 980 990 1000
SMQVEDGEVF FKEEINMEPN YSETMFKKAK TRAKKKPRKR SDSSGGYTLS
1010 1020 1030 1040 1050
DVIQSPPSAG LLKSAKTNSV ESLPELLTSD SEGSYAGVAS PRDLQSPDFT
1060 1070 1080 1090 1100
AGFHSDKVEG KAKPYVNGIP PPCTREDVKP WEKSPTTKSA PQFIPSNRVD
1110 1120 1130 1140 1150
TAASSSWLAG SCSPVSPPVV DLRTIMETEE NRQKYGAAPK SNLGKIISHG
1160 1170 1180 1190 1200
IKLSQKQRKM IALTTKENNS GTNSMEAILT APSKSPKPAN AWAPLHSPLS
1210 1220 1230 1240 1250
RSFRDFLLEE KKPVPGYGSG DHVKKVCFKG TENSPALNVA RCSTHGTPGL
1260 1270 1280 1290 1300
ESNHVSDFPL LDSPNPWQSS SLAASPAVAP VTFASIVEEE RQQEAALIRS
1310 1320 1330 1340 1350
REKPLALIQV EEHAIQDLLV FYEAFGNPEE FVVVERAPQG PLAVPMWNKH

GC
Length:1,352
Mass (Da):149,569
Last modified:July 27, 2011 - v3
Checksum:i5FA4BC368281D734
GO
Isoform 2 (identifier: Q6ZPR6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-237: Missing.

Note: No experimental confirmation available.
Show »
Length:1,251
Mass (Da):138,576
Checksum:iCAB5A6660438838A
GO

Sequence cautioni

The sequence BAC36779.1 differs from that shown. Reason: Frameshift at position 1153. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti282 – 2821K → E in BAE20460 (PubMed:16141072).Curated
Sequence conflicti1189 – 11891A → V in BAC98163 (PubMed:14621295).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei137 – 237101Missing in isoform 2. 1 PublicationVSP_023605Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466522 Genomic DNA. Translation: EDL26483.1.
BC137798 mRNA. Translation: AAI37799.1.
AK030895 mRNA. Translation: BAE20460.1.
AK077387 mRNA. Translation: BAC36779.1. Frameshift.
AK129353 mRNA. Translation: BAC98163.1.
CCDSiCCDS40712.1. [Q6ZPR6-1]
RefSeqiNP_001074751.1. NM_001081282.2. [Q6ZPR6-1]
UniGeneiMm.22315.
Mm.487092.

Genome annotation databases

EnsembliENSMUST00000039213; ENSMUSP00000041145; ENSMUSG00000035941. [Q6ZPR6-1]
GeneIDi108837.
KEGGimmu:108837.
UCSCiuc009qww.2. mouse. [Q6ZPR6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466522 Genomic DNA. Translation: EDL26483.1.
BC137798 mRNA. Translation: AAI37799.1.
AK030895 mRNA. Translation: BAE20460.1.
AK077387 mRNA. Translation: BAC36779.1. Frameshift.
AK129353 mRNA. Translation: BAC98163.1.
CCDSiCCDS40712.1. [Q6ZPR6-1]
RefSeqiNP_001074751.1. NM_001081282.2. [Q6ZPR6-1]
UniGeneiMm.22315.
Mm.487092.

3D structure databases

ProteinModelPortaliQ6ZPR6.
SMRiQ6ZPR6. Positions 48-422, 776-900.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224442. 1 interaction.
STRINGi10090.ENSMUSP00000041145.

PTM databases

iPTMnetiQ6ZPR6.
PhosphoSiteiQ6ZPR6.

Proteomic databases

EPDiQ6ZPR6.
MaxQBiQ6ZPR6.
PaxDbiQ6ZPR6.
PRIDEiQ6ZPR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039213; ENSMUSP00000041145; ENSMUSG00000035941. [Q6ZPR6-1]
GeneIDi108837.
KEGGimmu:108837.
UCSCiuc009qww.2. mouse. [Q6ZPR6-1]

Organism-specific databases

CTDi25998.
MGIiMGI:1918677. Ibtk.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0783. Eukaryota.
COG5184. LUCA.
GeneTreeiENSGT00830000128260.
HOGENOMiHOG000090207.
HOVERGENiHBG081779.
InParanoidiQ6ZPR6.
OMAiVSALHHK.
OrthoDBiEOG7K6PT6.
TreeFamiTF323747.

Miscellaneous databases

ChiTaRSiIbtk. mouse.
NextBioi361375.
PROiQ6ZPR6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZPR6.
ExpressionAtlasiQ6ZPR6. baseline and differential.
GenevisibleiQ6ZPR6. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.130.10.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 2 hits.
PF00415. RCC1. 3 hits.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50985. SSF50985. 1 hit.
SSF54695. SSF54695. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50097. BTB. 2 hits.
PS50012. RCC1_3. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-742 AND 1097-1352 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head and Thymus.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-1352 (ISOFORM 2).
    Tissue: Embryonic tail.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1046, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1005; SER-1046; SER-1111; SER-1113 AND SER-1116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiIBTK_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPR6
Secondary accession number(s): B9EHE9, Q3V3X0, Q8BVL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.