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Protein

Sphingomyelin phosphodiesterase 4

Gene

Smpd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. May sensitize cells to DNA damage-induced apoptosis.By similarity

Caution

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphatidylserine and tumor necrosis factor (TNF). Inhibited by scyphostatin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism

Chemistry databases

SwissLipidsiSLP:000001481 [Q6ZPR5-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesterase 4 (EC:3.1.4.12)
Alternative name(s):
Neutral sphingomyelinase 3
Short name:
nSMase-3
Short name:
nSMase3
Neutral sphingomyelinase III
Gene namesi
Name:Smpd4
Synonyms:Kiaa1418
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1924876 Smpd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei776 – 796HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002731641 – 823Sphingomyelin phosphodiesterase 4Add BLAST823

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Modified residuei245PhosphoserineCombined sources1
Modified residuei665PhosphothreonineBy similarity1
Modified residuei749PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6ZPR5
PeptideAtlasiQ6ZPR5
PRIDEiQ6ZPR5

PTM databases

iPTMnetiQ6ZPR5
PhosphoSitePlusiQ6ZPR5

Expressioni

Gene expression databases

BgeeiENSMUSG00000005899
CleanExiMM_SMPD4
ExpressionAtlasiQ6ZPR5 baseline and differential
GenevisibleiQ6ZPR5 MM

Interactioni

Protein-protein interaction databases

BioGridi2188082 interactors.
IntActiQ6ZPR5 2 interactors.
STRINGi10090.ENSMUSP00000006053

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4396 Eukaryota
ENOG410YYEJ LUCA
GeneTreeiENSGT00390000006044
HOVERGENiHBG082060
InParanoidiQ6ZPR5
KOiK12353
OMAiLNPHQMS
OrthoDBiEOG091G052W
PhylomeDBiQ6ZPR5
TreeFamiTF324409

Family and domain databases

InterProiView protein in InterPro
IPR024129 Sphingomy_SMPD4
PANTHERiPTHR12988 PTHR12988, 1 hit
PfamiView protein in Pfam
PF14724 mit_SMPDase, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZPR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFPHLQQPS FLLASLKADS INKPFAQRCQ DLVKVIEDFP AKELHAVFPW
60 70 80 90 100
LVESIFGSLD GVLVGWNLRC LQGRVNPVEY STAMEFLDPS GPMMKLVYKL
110 120 130 140 150
QAEDYNFDFP VSCLPGPVKA SIQENVLPDS PLYHNKVQFP PTGGLGLNLA
160 170 180 190 200
LNPFEYYMFY FALSLISQKP MSMTLHVRTS DCAYFTLVDR YLSWFLPTEG
210 220 230 240 250
SVPPPLCSSP GGSSPSPAPR TPAMPFASYG LHTSLLKRHI SHQTSVNADP
260 270 280 290 300
ASHEIWRSET LLQVFVEMWL HHYSLEMYQK MQSPHAKLEV LHYRLTVSSA
310 320 330 340 350
LHSPAQPSLQ ALHAYQESFT PTEEHVLVVR LLLKHLHAFA NSLKPDQASP
360 370 380 390 400
SAHSHATSPL EEFKRAAVPR FVQQKLYVFL QHCFGHWPLD ATFRAVLEMW
410 420 430 440 450
LSYLQPWRYA PEKQAQGSDP QPRCVSEKWA PFIQENLLMY TKLFVSFLNR
460 470 480 490 500
ALRTDLVSPK NALMVFRVAK VFAQPNLAEM IQKGEQLFLE PELIIPHRQH
510 520 530 540 550
RLFTVTTSFL SPWPPVVTDA SFKVKSHVYS LEGQDCKYTP MFGPEIRTLV
560 570 580 590 600
LRLAQLITQA KQTAKSISDQ YVESPTGRSF LSWLTFGLTD TNSCYPANDL
610 620 630 640 650
DEIGQDSIRK TDEYLEKALE YLRQIFRLSE AQLAQLTLAL GSARDENGKQ
660 670 680 690 700
QLPDCIVGEE GLILTPLGRY QIINGLRRFE IEYQGDLELQ PIRSYEITSL
710 720 730 740 750
VRALFRLSSA INRRFAGQMA ALCSRNDFLG SFCRYHLTEP ALSNRHLLSP
760 770 780 790 800
VGRRQVTNPA RGPRLSLRFL GSYRTLLLLL MAFFVASLFC IGPLSCSLLL
810 820
VLGYVLYAIA MTLLTERGKL HQL
Length:823
Mass (Da):93,276
Last modified:January 23, 2007 - v2
Checksum:i3C082D335608BA70
GO
Isoform 2 (identifier: Q6ZPR5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-316: Missing.

Show »
Length:794
Mass (Da):90,063
Checksum:i326A83F65CEF2603
GO
Isoform 3 (identifier: Q6ZPR5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-316: Missing.
     429-429: Missing.

Note: No experimental confirmation available.
Show »
Length:793
Mass (Da):89,877
Checksum:i78B7FB3C9954C1B1
GO
Isoform 4 (identifier: Q6ZPR5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-316: Missing.
     429-429: W → CLAPWSPYQHRGLR

Note: No experimental confirmation available.
Show »
Length:807
Mass (Da):91,543
Checksum:i2DF7496773312AFD
GO
Isoform 5 (identifier: Q6ZPR5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.
     288-316: Missing.

Note: No experimental confirmation available.
Show »
Length:637
Mass (Da):72,493
Checksum:i188248B501580BB4
GO

Sequence cautioni

The sequence BAC98164 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Q → P in AAH26767 (PubMed:15489334).Curated1
Sequence conflicti15S → T in BAC27221 (PubMed:16141072).Curated1
Sequence conflicti141P → T in BAC27512 (PubMed:16141072).Curated1
Sequence conflicti195F → V in AAH26767 (PubMed:15489334).Curated1
Sequence conflicti368V → E in BAB31737 (PubMed:16141072).Curated1
Sequence conflicti732F → Y in BAC27221 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0224841 – 157Missing in isoform 5. 1 PublicationAdd BLAST157
Alternative sequenceiVSP_022485288 – 316Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST29
Alternative sequenceiVSP_022486429Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_022487429W → CLAPWSPYQHRGLR in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129354 mRNA Translation: BAC98164.1 Different initiation.
AK019466 mRNA Translation: BAB31737.1
AK031029 mRNA Translation: BAC27221.1
AK031685 mRNA Translation: BAC27512.1
AK035892 mRNA Translation: BAC29229.1
AK164234 mRNA Translation: BAE37694.1
AK164456 mRNA Translation: BAE37796.1
BC026767 mRNA Translation: AAH26767.1
CCDSiCCDS28009.1 [Q6ZPR5-1]
CCDS49779.1 [Q6ZPR5-3]
CCDS49780.1 [Q6ZPR5-4]
CCDS49781.1 [Q6ZPR5-2]
RefSeqiNP_001158081.1, NM_001164609.1 [Q6ZPR5-4]
NP_001158082.1, NM_001164610.1 [Q6ZPR5-2]
NP_001158083.1, NM_001164611.1 [Q6ZPR5-3]
NP_084221.2, NM_029945.3 [Q6ZPR5-1]
XP_006522785.1, XM_006522722.2 [Q6ZPR5-1]
UniGeneiMm.34659

Genome annotation databases

EnsembliENSMUST00000006053; ENSMUSP00000006053; ENSMUSG00000005899 [Q6ZPR5-1]
ENSMUST00000090159; ENSMUSP00000087620; ENSMUSG00000005899 [Q6ZPR5-4]
ENSMUST00000163476; ENSMUSP00000131867; ENSMUSG00000005899 [Q6ZPR5-2]
ENSMUST00000165363; ENSMUSP00000130720; ENSMUSG00000005899 [Q6ZPR5-3]
GeneIDi77626
KEGGimmu:77626
UCSCiuc007yll.2 mouse [Q6ZPR5-1]
uc007ylm.2 mouse [Q6ZPR5-3]
uc007yln.2 mouse [Q6ZPR5-4]
uc007ylo.2 mouse [Q6ZPR5-2]
uc007ylp.2 mouse [Q6ZPR5-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNSMA3_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPR5
Secondary accession number(s): Q3TPE0
, Q3TPP1, Q8CBJ5, Q8CD12, Q8CD83, Q8R0J3, Q9CX74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome