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Protein

Lateral signaling target protein 2 homolog

Gene

Zfyve28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri835 – 89561FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Lateral signaling target protein 2 homolog
Alternative name(s):
Zinc finger FYVE domain-containing protein 28
Gene namesi
Name:Zfyve28
Synonyms:Kiaa1643, Lst2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2684992. Zfyve28.

Subcellular locationi

  • Cytoplasmcytosol
  • Early endosome membrane

  • Note: Localizes to early endosome membrane in absence of Lys-87 monoubiquitination. Localizes to cytosol when monoubiquitinated (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 905905Lateral signaling target protein 2 homologPRO_0000378953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki87 – 87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei512 – 5121PhosphothreonineBy similarity
Modified residuei888 – 8881Phosphothreonine; by MAP2KBy similarity

Post-translational modificationi

Monoubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ6ZPK7.
PaxDbiQ6ZPK7.
PRIDEiQ6ZPK7.

PTM databases

iPTMnetiQ6ZPK7.
PhosphoSiteiQ6ZPK7.

Expressioni

Tissue specificityi

Enriched in brain (at protein level).1 Publication

Gene expression databases

BgeeiQ6ZPK7.
ExpressionAtlasiQ6ZPK7. baseline and differential.
GenevisibleiQ6ZPK7. MM.

Interactioni

Subunit structurei

Interacts with TRIM3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092464.

Structurei

3D structure databases

ProteinModelPortaliQ6ZPK7.
SMRiQ6ZPK7. Positions 772-891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not monoubiquitinated at Lys-87.By similarity

Sequence similaritiesi

Belongs to the lst-2 family.Curated
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri835 – 89561FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1819. Eukaryota.
ENOG410XNYQ. LUCA.
GeneTreeiENSGT00840000129741.
HOGENOMiHOG000113403.
InParanoidiQ6ZPK7.
OMAiFGWAGST.
OrthoDBiEOG7RFTGP.
PhylomeDBiQ6ZPK7.
TreeFamiTF320752.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ZPK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNRFRKWLY KPKRSDPQLL AQFYYADEEL NQVAAELDSL DGRKEPQRCT
60 70 80 90 100
LLVSQFRSCQ DNVLNIINQI MEECIPQDRA PRDFCVKFPE EIRHDNLAGQ
110 120 130 140 150
LWFGAECLAA GSIIMNRELE SMAMRPLAKE LTRSLEDVRG TLRDQALRDL
160 170 180 190 200
NTYTEKMREA LRRFDVLFAE FELSYVSAMV PVKSPREYYV QQEVIVLFCE
210 220 230 240 250
TVERALDFGY LTQDMIDDYE PALMFTIPRL AIVCGLVVYA DGPLNLDRKV
260 270 280 290 300
EDMSELFRPF HTLLRKIRDL LQALTEEELH TLERSLCVSQ DVELPIRADT
310 320 330 340 350
QAPSALAPTF SASLPPEETL SASANNPEAE LACSMQYDDQ ELEELSRMVH
360 370 380 390 400
RAGDEMSSLL SPPSACQSPA HRPGSEASPR GEASPARARL KSGSDEEERV
410 420 430 440 450
FFMDDVEVTE SPARPESPGN TFELTQGNAQ QRGQDGQSGE VGVEAPALVK
460 470 480 490 500
EEDWSNNNVE GDKIKLASLM GSTSCSCLDS QLYLDGWEVS AEDAETAEMI
510 520 530 540 550
AHRTGGMKLS ATVIFNPKSP TSQDSAVAAQ EAPGHGTSPL EPRAEGTGDN
560 570 580 590 600
SHKLSTTATN CLLHSCVCCG SCGDSRDDAV ERLREKCGPG SVISASNPSV
610 620 630 640 650
SLAKGGDKEP ERIDEAQPSD VTLPAEDASN RQEPKAPASS KCLAHTSGPQ
660 670 680 690 700
VDTASRLQGE GEVKGQPEPE ARKQDPEKSP VVSGDSPRGD VAQTEHQHLL
710 720 730 740 750
GSSSTVGSCS LDNTRLDVAT AAMPVTPMAT REKIRSRFHG SHDLIHRLFV
760 770 780 790 800
CISGVADQLQ TNYASDLRSI LKTLFEVMAT KPETDDKEKL KKVTQTLRSA
810 820 830 840 850
ALEDCALCQE TVSSSELAAK TRDGDFEDPP EWVPDEACGF CTSCKAPFTV
860 870 880 890 900
IRRKHHCRSC GKIFCSRCSS HSAPLPRYGQ VKPVRVCTHC YMFHVTPFYS

DKTGM
Length:905
Mass (Da):99,787
Last modified:July 7, 2009 - v2
Checksum:iE713EAB3E4DB0FEB
GO

Sequence cautioni

The sequence BAC98224.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129414 mRNA. Translation: BAC98224.1. Different initiation.
BC139050 mRNA. Translation: AAI39051.1.
BC139051 mRNA. Translation: AAI39052.1.
CCDSiCCDS39068.1.
RefSeqiNP_001015039.1. NM_001015039.1.
UniGeneiMm.113388.

Genome annotation databases

EnsembliENSMUST00000094868; ENSMUSP00000092464; ENSMUSG00000037224.
GeneIDi231125.
KEGGimmu:231125.
UCSCiuc008xca.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129414 mRNA. Translation: BAC98224.1. Different initiation.
BC139050 mRNA. Translation: AAI39051.1.
BC139051 mRNA. Translation: AAI39052.1.
CCDSiCCDS39068.1.
RefSeqiNP_001015039.1. NM_001015039.1.
UniGeneiMm.113388.

3D structure databases

ProteinModelPortaliQ6ZPK7.
SMRiQ6ZPK7. Positions 772-891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092464.

PTM databases

iPTMnetiQ6ZPK7.
PhosphoSiteiQ6ZPK7.

Proteomic databases

MaxQBiQ6ZPK7.
PaxDbiQ6ZPK7.
PRIDEiQ6ZPK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094868; ENSMUSP00000092464; ENSMUSG00000037224.
GeneIDi231125.
KEGGimmu:231125.
UCSCiuc008xca.1. mouse.

Organism-specific databases

CTDi57732.
MGIiMGI:2684992. Zfyve28.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1819. Eukaryota.
ENOG410XNYQ. LUCA.
GeneTreeiENSGT00840000129741.
HOGENOMiHOG000113403.
InParanoidiQ6ZPK7.
OMAiFGWAGST.
OrthoDBiEOG7RFTGP.
PhylomeDBiQ6ZPK7.
TreeFamiTF320752.

Miscellaneous databases

ChiTaRSiZfyve28. mouse.
PROiQ6ZPK7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZPK7.
ExpressionAtlasiQ6ZPK7. baseline and differential.
GenevisibleiQ6ZPK7. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling."
    Mosesson Y., Chetrit D., Schley L., Berghoff J., Ziv T., Carvalho S., Milanezi F., Admon A., Schmitt F., Ehrlich M., Yarden Y.
    Dev. Cell 16:687-698(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLST2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPK7
Secondary accession number(s): B2RSX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 7, 2009
Last modified: July 6, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.