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Q6ZPF4 (FMNL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formin-like protein 3
Gene names
Name:Fmnl3
Synonyms:Kiaa2014
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1028 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration By similarity.

Subunit structure

Interacts with SRGAP2 (via SH3 domain). Ref.4

Subcellular location

Cytoplasm By similarity. Note: Enriched in lamellipodia By similarity.

Domain

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.

Sequence similarities

Belongs to the formin homology family.

Contains 1 DAD (diaphanous autoregulatory) domain.

Contains 1 FH2 (formin homology 2) domain.

Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

Sequence caution

The sequence BAC98283.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6ZPF4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6ZPF4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     152-202: Missing.
     999-1028: VLKSVPFTARTAKRGSRFFCDAAHHDESNC → GFNHQRMVVHSQVRSAVPPSGPPRAPGPH

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 10281027Formin-like protein 3
PRO_0000289096

Regions

Domain26 – 472447GBD/FH3
Domain561 – 951391FH2
Domain986 – 101833DAD
Compositional bias499 – 54850Pro-rich

Amino acid modifications

Modified residue1741Phosphoserine By similarity
Lipidation21N-myristoyl glycine By similarity

Natural variations

Alternative sequence152 – 20251Missing in isoform 2.
VSP_025894
Alternative sequence999 – 102830VLKSV…DESNC → GFNHQRMVVHSQVRSAVPPS GPPRAPGPH in isoform 2.
VSP_025895

Secondary structure

........................................... 1028
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: BBA0436746934A3B

FASTA1,028117,169
        10         20         30         40         50         60 
MGNLESTDGG PGEPPSVPLL LPPGKTPMPE PCELEERFAL VLSSMNLPPD KARLLRQYDN 

        70         80         90        100        110        120 
EKKWDLICDQ ERFQVKNPPH TYIQKLQSFL DPNVTRKKFR RRVQESTKVL RELEISLRTN 

       130        140        150        160        170        180 
HIGWVREFLN DENKGLDVLV DYLSFAQCSV MFDFEGLESG DDGAFDKLRS WSRSIEDLQP 

       190        200        210        220        230        240 
PNALSAPFTN SLARSARQSV LRYSTLPGRR ALKNSRLVSQ KDDVHVCILC LRAIMNYQYG 

       250        260        270        280        290        300 
FNLVMSHPHA VNEIALSLNN KNPRTKALVL ELLAAVCLVR GGHEIILAAF DNFKEVCKEL 

       310        320        330        340        350        360 
HRFEKLMEYF RNEDSNIDFM VACMQFINIV VHSVEDMNFR VHLQYEFTKL GLEEFLQKSR 

       370        380        390        400        410        420 
HTESEKLQVQ IQAYLDNVFD VGGLLEDAET KNVALEKVEE LEEHVSHLTE KLLDLENENM 

       430        440        450        460        470        480 
MRVAELEKQL LQREKELESI KETYENTSNQ VHTLRRLIKE KEEAFQRRCH LEPSARGLES 

       490        500        510        520        530        540 
MGGEALARVG PTELTEGIPP SDLDLLAPAP PTEETLPLPP PPAPPLPPPP PPLPDKCPPA 

       550        560        570        580        590        600 
PPLPGAAPSV VLTVGLSAIR IKKPIKTKFR LPVFNWTALK PNQINGTVFS ELDDEKILED 

       610        620        630        640        650        660 
LDLDRFEELF KTKAQGPALD LICSKNKTAQ KAASKVTLLE ANRAKNLAIT LRKAGRSAEE 

       670        680        690        700        710        720 
ICRAIHTFDL QTLPVDFVEC LMRFLPTEAE VKLLRQYERE RQPLEELAAE DRFMLLFSKV 

       730        740        750        760        770        780 
ERLTQRMAGM AFLGNFQDNL QMLTPQLNAI IAASASVKSS QKLKQMLEII LALGNYMNSS 

       790        800        810        820        830        840 
KRGAVYGFKL QSLDLLLDTK STDRKMTLLH FIALTVKEKY PELANFWQEL HFVEKAAAVS 

       850        860        870        880        890        900 
LENVLLDVKE LGRGMELIRR ECSIHDNSVL RNFLSTNEGK LDKLQRDAKT AEEAYNAVVR 

       910        920        930        940        950        960 
YFGESPKTTP PSVFFPVFVR FIRSYKEAEQ ENEARKKQEE VMREKQLAQE AKKLDAKTPS 

       970        980        990       1000       1010       1020 
QRNKWQQQEL IAELRRRQAK EHRPVYEGKD GTIEDIITVL KSVPFTARTA KRGSRFFCDA 


AHHDESNC 

« Hide

Isoform 2 [UniParc].

Checksum: 702206CD74A93F8B
Show »

FASTA976111,279

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryonic tail.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: NOD.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"Bi-modal regulation of a formin by srGAP2."
Mason F.M., Heimsath E.G., Higgs H.N., Soderling S.H.
J. Biol. Chem. 286:6577-6586(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRGAP2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK129473 mRNA. Translation: BAC98283.1. Different initiation.
AK155331 mRNA. Translation: BAE33197.1.
AK170744 mRNA. Translation: BAE41997.1.
BC131961 mRNA. Translation: AAI31962.1.
RefSeqNP_035841.1. NM_011711.1.
XP_006520908.1. XM_006520845.1.
UniGeneMm.29043.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EAHX-ray3.40A/B/C/E555-954[»]
ProteinModelPortalQ6ZPF4.
SMRQ6ZPF4. Positions 79-422, 551-1007.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-32010N.
IntActQ6ZPF4. 3 interactions.

PTM databases

PhosphoSiteQ6ZPF4.

Proteomic databases

PaxDbQ6ZPF4.
PRIDEQ6ZPF4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081224; ENSMUSP00000079984; ENSMUSG00000023008. [Q6ZPF4-2]
ENSMUST00000088233; ENSMUSP00000085566; ENSMUSG00000023008. [Q6ZPF4-1]
GeneID22379.
KEGGmmu:22379.
UCSCuc007xpj.1. mouse. [Q6ZPF4-1]
uc007xpl.1. mouse. [Q6ZPF4-2]

Organism-specific databases

CTD91010.
MGIMGI:109569. Fmnl3.
RougeSearch...

Phylogenomic databases

eggNOGNOG149898.
GeneTreeENSGT00710000106395.
HOGENOMHOG000231209.
HOVERGENHBG053118.
InParanoidQ6ZPF4.
OMARCHLEPN.
OrthoDBEOG7F7W8J.
PhylomeDBQ6ZPF4.
TreeFamTF325155.

Gene expression databases

ArrayExpressQ6ZPF4.
BgeeQ6ZPF4.
CleanExMM_FMNL3.
GenevestigatorQ6ZPF4.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR027655. Fmnl3.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PANTHERPTHR23213:SF29. PTHR23213:SF29. 1 hit.
PfamPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 2 hits.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
PROSITEPS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFMNL3. mouse.
NextBio302737.
PROQ6ZPF4.
SOURCESearch...

Entry information

Entry nameFMNL3_MOUSE
AccessionPrimary (citable) accession number: Q6ZPF4
Secondary accession number(s): Q3TCF9, Q3U2E4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot