Q6ZPE2 (MTMR5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myotubularin-related protein 5

Alternative name(s):
SET-binding factor 1
Short name=Sbf1

Gene names

Name:	Sbf1
Synonyms:	Kiaa3020, Mtmr5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1867 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. Alternatively proposed to function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form By similarity.
Subunit structure	Interacts with the SET domain of MLL/HRX. Interacts with SUV39H1 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. Contains 1 dDENN domain. Contains 1 DENN domain. Contains 1 GRAM domain. Contains 1 myotubularin phosphatase domain. Contains 1 PH domain. Contains 1 uDENN domain.
Sequence caution	The sequence AAH29156.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC36139.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC98295.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell growth Traceable author statement PubMed 11994405. Source: MGI spermatogenesis Inferred from mutant phenotype PubMed 11994405. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW phosphatase activity Inferred from electronic annotation. Source: InterPro phospholipid binding Inferred from electronic annotation. Source: InterPro pseudophosphatase activity Traceable author statement PubMed 11994405. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1867

1867

Myotubularin-related protein 5

PRO_0000293476

Regions

Domain

18 – 86

69

UDENN

Domain

128 – 310

183

DENN

Domain

363 – 432

70

dDENN

Domain

880 – 968

89

GRAM

Domain

1120 – 1596

477

Myotubularin phosphatase

Domain

1761 – 1865

105

PH

Amino acid modifications

Modified residue

1137

1

Phosphothreonine By similarity

Modified residue

1749

1

Phosphothreonine Ref.4

Secondary structure

1

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1867

Helix Strand Turn

Details...

Beta strand

1762 – 1770

9

Beta strand

1774 – 1776

3

Beta strand

1779 – 1786

8

Turn

1787 – 1790

4

Beta strand

1791 – 1799

9

Beta strand

1806 – 1808

3

Helix

1809 – 1811

3

Beta strand

1812 – 1816

5

Beta strand

1826 – 1828

3

Turn

1830 – 1832

3

Beta strand

1834 – 1840

7

Beta strand

1842 – 1846

5

Helix

1850 – 1861

12

Sequences

	Sequence		Length	Mass (Da)
	Q6ZPE2 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 16C7033FAA62CB1A	FASTA	1,867	208,693
	10 20 30 40 50 60 MARLADYFVL VAFGPHPRGS GEGQGQILQR FPEKDWEDNP FPQGIELFCQ PSGWQLCPER 70 80 90 100 110 120 NPPTFFVAVL TDINSERHYC ACLTFWEPVE STQEVVCTDN ATEKEEEADG GGQARLSSTA 130 140 150 160 170 180 PAQPGQLFAP KTLVLVSRLD HAEVFRNSLG LIYAIHVEGL NVSLENVIGN LLTCTVPLAG 190 200 210 220 230 240 GSQRTISLGA GDRQVIQTPL VDSLPVSRCS VALLFRQLGI TNVLSLFCAA LTEHKVLFLS 250 260 270 280 290 300 RSYQRLADAC RGLLALLFPL RYSFTYVPIL PAQLLEVLST PTPFIIGVNA AFQAETQELL 310 320 330 340 350 360 DVIVADLDGG TVTVPECVHI PPLPEPLQSQ THNVLSMVLD PELELADLAF PPPTTSASSL 370 380 390 400 410 420 KMQDKELRAV FLRLFAQLLQ GYRWCLHIVR IHPEPVIRFH KAAFLGQRGL VEDDFLMKVL 430 440 450 460 470 480 EGMAFAGFVS ERGVPYRATD LFDELVAHEV ARMRADESHP HRVLRHVQEL AEQLYKNENP 490 500 510 520 530 540 YPAVAMHKVQ RPGEASHLRR THRPFPRLDE GTIQWIVDQA AAKMQGAPPA VKAERRSTVP 550 560 570 580 590 600 SGPPMTAILE RCSGPHINSA RRLEVVRNCI SYVFEGKMLE AKKLLPAVLR ALKGRAARRC 610 620 630 640 650 660 LAHELHLHVQ QNRAVLDHQQ FDFVVRMMNC CLQDCTSLDE HGIASALLPL VTAFCRKLSP 670 680 690 700 710 720 GVTQFAYSCV QEHVVWSTPQ FWEAMFYGDV QTHIRALYLE PSDGVSPTQE TGEAQSQDDE 730 740 750 760 770 780 RSALDVASEQ RRLWPTLSRE KQQELVQKEE STVFSQAIHY ANRMSYLLLP LDSSKSRLLR 790 800 810 820 830 840 ERAGLGDLES ASNSLVTNSM AGSVAESYDT ESGFEDAETC DVAGAVVRFI NRFVDKVCTE 850 860 870 880 890 900 SGVTSDHLKG LHVMVPDIVQ MHIETLEAVH RESKRLPPIQ KPKLLRPRLL PGEECVLDGL 910 920 930 940 950 960 RVYLLPDGRE EGVGGSGGGP ALLPAEGAVF LTTYRVIFTG MPTDPLVGEQ VVVRSFPVAA 970 980 990 1000 1010 1020 LTKEKRISVQ TPVDQLLQDG LQLRSCTFQL LKMAFDEEVG SDSAELFRKQ LHKLRYPPDI 1030 1040 1050 1060 1070 1080 RATFAFTLGS AHTPGRPPRV TKDKGPSFRT LSRNLMKNAK KTIGRQYVTR KKYNPPGWEH 1090 1100 1110 1120 1130 1140 RGQPPPEDQE DEISVSEELE PSTLTPSSAL KPSDRMTMSS LVERACCRDY QRLGLGTLSS 1150 1160 1170 1180 1190 1200 SLSRAKSEPF RISPVNRMYA ICRSYPGLLI VPQSIQDNAL QRVSRCYRQN RFPVVCWRSG 1210 1220 1230 1240 1250 1260 RSKAVLLRSG GLHGKGVVGL FKAQNTPSPG QAQADSSSLE QEKYLQAVVS SMPRYADSSG 1270 1280 1290 1300 1310 1320 RNTLSSFSSA HMGGHGKWSS VRASGRSSGL GSDVGSRLAG RDLLSTPHTN GAPPDSGFLR 1330 1340 1350 1360 1370 1380 PQRAALYIIG DKAQLKGVRP DPLQQWELVP IEVFEARQVK ASFKKLLKAC VPGCPATEPS 1390 1400 1410 1420 1430 1440 PASFLRSLED SEWLIQIHKL LQISVLVVEL LDSGSSVLVS LEDGWDITTQ VVSLVQLLSD 1450 1460 1470 1480 1490 1500 PFYRTLEGFR LLVEKEWLSF GHRFSHRGAH TLAGQSSGFT PVFLQFLDCV HQVHLQFPME 1510 1520 1530 1540 1550 1560 FEFSQFYLKF LGYHHTSRRF RTFLLDSDYE RIELGLLYEE KGERRGQLAC KSVWEYVDRL 1570 1580 1590 1600 1610 1620 SKRTPMFYNY TYAPEDTEVL RPYSNVSNLK VWDFYTEETL AEGPPYDWEL AQGPPEPPEE 1630 1640 1650 1660 1670 1680 ERPDGGAPQS RRRVVWPCYD SRPRVQPDAI SRLLEELQRL ETELGRPSER WKDTWDRVKA 1690 1700 1710 1720 1730 1740 AQRLESRQDG RGTPSSLLVS AVPHHRRSLG VYLQEGPVGS TLSLSLDSDQ SSGSTTSSSR 1750 1760 1770 1780 1790 1800 QAARRSTSTL YSQFQTAESE NRSYEGILYK KGAFMKPWKA RWFVLDKTKH QLRYYDHRMD 1810 1820 1830 1840 1850 1860 TECKGVIDLA EVEAVAPGTP TIGAPKTVDE KAFFDVKTTR RVYNFCAQDV PSAQQWVDRI QSCLSDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 822-1867. Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary tumor.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1522-1867. Strain: C57BL/6J. Tissue: Embryo.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1749, MASS SPECTROMETRY. Tissue: Liver.
[5]	"solution structure of the C-terminal pleckstrin homology domain of Sbf1 from mouse." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1762-1865.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK129485 mRNA. Translation: BAC98295.1. Different initiation.
BC029156 mRNA. Translation: AAH29156.1. Different initiation.
BC098209 mRNA. Translation: AAH98209.1.
AK076039 mRNA. Translation: BAC36139.1. Different initiation.

IPI

IPI00875821.

RefSeq

NP_001074499.2. NM_001081030.2.

UniGene

Mm.35483.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V5U	NMR	-	A	1762-1865	[»]

ProteinModelPortal

Q6ZPE2.

SMR

Q6ZPE2. Positions 27-432, 1147-1251, 1318-1595, 1764-1865.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6ZPE2. 3 interactions.

PTM databases

PhosphoSite

Q6ZPE2.

Proteomic databases

PaxDb

Q6ZPE2.

PRIDE

Q6ZPE2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000123791; ENSMUSP00000120725; ENSMUSG00000036529.

GeneID

77980.

KEGG

mmu:77980.

UCSC

uc007xfz.1. mouse.

Organism-specific databases

CTD

6305.

MGI

MGI:1925230. Sbf1.

Rouge

Search...

Phylogenomic databases

eggNOG

NOG315534.

GeneTree

ENSGT00700000104053.

HOGENOM

HOG000044360.

HOVERGEN

HBG052527.

InParanoid

Q6ZPE2.

Gene expression databases

ArrayExpress

Q6ZPE2.

Bgee

Q6ZPE2.

CleanEx

MM_SBF1.

Genevestigator

Q6ZPE2.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR004182. GRAM.
IPR010569. Myotub-related.
IPR017906. Myotubularin_phosphatase_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR022096. SBF2.
IPR005113. uDENN_dom.
[Graphical view]

Pfam

PF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
PF00169. PH. 1 hit.
PF12335. SBF2. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]

SMART

SM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00568. GRAM. 1 hit.
SM00233. PH. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]

PROSITE

PS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51339. PPASE_MYOTUBULARIN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SBF1. mouse.

EvolutionaryTrace

Q6ZPE2.

NextBio

347929.

SOURCE

Search...

Entry information

Entry name

MTMR5_MOUSE

Accession

Primary (citable) accession number: Q6ZPE2
Secondary accession number(s): Q4QQM2, Q8BK68, Q8K2Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program