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Q6ZPE2 (MTMR5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myotubularin-related protein 5
Alternative name(s):
SET-binding factor 1
Short name=Sbf1
Gene names
Name:Sbf1
Synonyms:Kiaa3020, Mtmr5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1867 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. Alternatively proposed to function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form By similarity.

Subunit structure

Interacts with the SET domain of KMT2A/MLL1. Interacts with SUV39H1 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.

Contains 1 dDENN domain.

Contains 1 DENN domain.

Contains 1 GRAM domain.

Contains 1 myotubularin phosphatase domain.

Contains 1 PH domain.

Contains 1 uDENN domain.

Sequence caution

The sequence AAH29156.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC98295.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6ZPE2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6ZPE2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1275-1275: H → HVPSPRARVTTLSNPLAASASRWTASR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18671867Myotubularin-related protein 5
PRO_0000293476

Regions

Domain18 – 8669UDENN
Domain128 – 310183DENN
Domain363 – 43270dDENN
Domain880 – 96889GRAM
Domain1120 – 1596477Myotubularin phosphatase
Domain1761 – 1865105PH

Amino acid modifications

Modified residue11371Phosphothreonine By similarity

Natural variations

Alternative sequence12751H → HVPSPRARVTTLSNPLAASA SRWTASR in isoform 2.
VSP_047523

Experimental info

Sequence conflict3171C → R in AAI72094. Ref.3
Sequence conflict13471E → D in AAI57936. Ref.3
Sequence conflict15211R → W in BAC36139. Ref.4

Secondary structure

....................... 1867
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 16C7033FAA62CB1A

FASTA1,867208,693
        10         20         30         40         50         60 
MARLADYFVL VAFGPHPRGS GEGQGQILQR FPEKDWEDNP FPQGIELFCQ PSGWQLCPER 

        70         80         90        100        110        120 
NPPTFFVAVL TDINSERHYC ACLTFWEPVE STQEVVCTDN ATEKEEEADG GGQARLSSTA 

       130        140        150        160        170        180 
PAQPGQLFAP KTLVLVSRLD HAEVFRNSLG LIYAIHVEGL NVSLENVIGN LLTCTVPLAG 

       190        200        210        220        230        240 
GSQRTISLGA GDRQVIQTPL VDSLPVSRCS VALLFRQLGI TNVLSLFCAA LTEHKVLFLS 

       250        260        270        280        290        300 
RSYQRLADAC RGLLALLFPL RYSFTYVPIL PAQLLEVLST PTPFIIGVNA AFQAETQELL 

       310        320        330        340        350        360 
DVIVADLDGG TVTVPECVHI PPLPEPLQSQ THNVLSMVLD PELELADLAF PPPTTSASSL 

       370        380        390        400        410        420 
KMQDKELRAV FLRLFAQLLQ GYRWCLHIVR IHPEPVIRFH KAAFLGQRGL VEDDFLMKVL 

       430        440        450        460        470        480 
EGMAFAGFVS ERGVPYRATD LFDELVAHEV ARMRADESHP HRVLRHVQEL AEQLYKNENP 

       490        500        510        520        530        540 
YPAVAMHKVQ RPGEASHLRR THRPFPRLDE GTIQWIVDQA AAKMQGAPPA VKAERRSTVP 

       550        560        570        580        590        600 
SGPPMTAILE RCSGPHINSA RRLEVVRNCI SYVFEGKMLE AKKLLPAVLR ALKGRAARRC 

       610        620        630        640        650        660 
LAHELHLHVQ QNRAVLDHQQ FDFVVRMMNC CLQDCTSLDE HGIASALLPL VTAFCRKLSP 

       670        680        690        700        710        720 
GVTQFAYSCV QEHVVWSTPQ FWEAMFYGDV QTHIRALYLE PSDGVSPTQE TGEAQSQDDE 

       730        740        750        760        770        780 
RSALDVASEQ RRLWPTLSRE KQQELVQKEE STVFSQAIHY ANRMSYLLLP LDSSKSRLLR 

       790        800        810        820        830        840 
ERAGLGDLES ASNSLVTNSM AGSVAESYDT ESGFEDAETC DVAGAVVRFI NRFVDKVCTE 

       850        860        870        880        890        900 
SGVTSDHLKG LHVMVPDIVQ MHIETLEAVH RESKRLPPIQ KPKLLRPRLL PGEECVLDGL 

       910        920        930        940        950        960 
RVYLLPDGRE EGVGGSGGGP ALLPAEGAVF LTTYRVIFTG MPTDPLVGEQ VVVRSFPVAA 

       970        980        990       1000       1010       1020 
LTKEKRISVQ TPVDQLLQDG LQLRSCTFQL LKMAFDEEVG SDSAELFRKQ LHKLRYPPDI 

      1030       1040       1050       1060       1070       1080 
RATFAFTLGS AHTPGRPPRV TKDKGPSFRT LSRNLMKNAK KTIGRQYVTR KKYNPPGWEH 

      1090       1100       1110       1120       1130       1140 
RGQPPPEDQE DEISVSEELE PSTLTPSSAL KPSDRMTMSS LVERACCRDY QRLGLGTLSS 

      1150       1160       1170       1180       1190       1200 
SLSRAKSEPF RISPVNRMYA ICRSYPGLLI VPQSIQDNAL QRVSRCYRQN RFPVVCWRSG 

      1210       1220       1230       1240       1250       1260 
RSKAVLLRSG GLHGKGVVGL FKAQNTPSPG QAQADSSSLE QEKYLQAVVS SMPRYADSSG 

      1270       1280       1290       1300       1310       1320 
RNTLSSFSSA HMGGHGKWSS VRASGRSSGL GSDVGSRLAG RDLLSTPHTN GAPPDSGFLR 

      1330       1340       1350       1360       1370       1380 
PQRAALYIIG DKAQLKGVRP DPLQQWELVP IEVFEARQVK ASFKKLLKAC VPGCPATEPS 

      1390       1400       1410       1420       1430       1440 
PASFLRSLED SEWLIQIHKL LQISVLVVEL LDSGSSVLVS LEDGWDITTQ VVSLVQLLSD 

      1450       1460       1470       1480       1490       1500 
PFYRTLEGFR LLVEKEWLSF GHRFSHRGAH TLAGQSSGFT PVFLQFLDCV HQVHLQFPME 

      1510       1520       1530       1540       1550       1560 
FEFSQFYLKF LGYHHTSRRF RTFLLDSDYE RIELGLLYEE KGERRGQLAC KSVWEYVDRL 

      1570       1580       1590       1600       1610       1620 
SKRTPMFYNY TYAPEDTEVL RPYSNVSNLK VWDFYTEETL AEGPPYDWEL AQGPPEPPEE 

      1630       1640       1650       1660       1670       1680 
ERPDGGAPQS RRRVVWPCYD SRPRVQPDAI SRLLEELQRL ETELGRPSER WKDTWDRVKA 

      1690       1700       1710       1720       1730       1740 
AQRLESRQDG RGTPSSLLVS AVPHHRRSLG VYLQEGPVGS TLSLSLDSDQ SSGSTTSSSR 

      1750       1760       1770       1780       1790       1800 
QAARRSTSTL YSQFQTAESE NRSYEGILYK KGAFMKPWKA RWFVLDKTKH QLRYYDHRMD 

      1810       1820       1830       1840       1850       1860 
TECKGVIDLA EVEAVAPGTP TIGAPKTVDE KAFFDVKTTR RVYNFCAQDV PSAQQWVDRI 


QSCLSDA 

« Hide

Isoform 2 [UniParc].

Checksum: D4F26DD11889F5D6
Show »

FASTA1,893211,428

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1521-1867.
Strain: C57BL/6J.
Tissue: Embryo.
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"solution structure of the C-terminal pleckstrin homology domain of Sbf1 from mouse."
RIKEN structural genomics initiative (RSGI)
Submitted (MAY-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 1762-1865.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK129485 mRNA. Translation: BAC98295.1. Different initiation.
AC160538 Genomic DNA. No translation available.
BC029156 mRNA. Translation: AAH29156.1. Different initiation.
BC098209 mRNA. Translation: AAH98209.1.
BC157935 mRNA. Translation: AAI57936.1.
BC158013 mRNA. Translation: AAI58014.1.
BC172094 mRNA. Translation: AAI72094.1.
AK076039 mRNA. Translation: BAC36139.1.
RefSeqNP_001074499.2. NM_001081030.2.
NP_001164032.1. NM_001170561.1.
UniGeneMm.35483.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V5UNMR-A1762-1865[»]
ProteinModelPortalQ6ZPE2.
SMRQ6ZPE2. Positions 27-432, 885-1597, 1764-1865.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid219065. 2 interactions.
IntActQ6ZPE2. 3 interactions.

PTM databases

PhosphoSiteQ6ZPE2.

Proteomic databases

PaxDbQ6ZPE2.
PRIDEQ6ZPE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000123791; ENSMUSP00000120725; ENSMUSG00000036529. [Q6ZPE2-1]
ENSMUST00000144585; ENSMUSP00000118107; ENSMUSG00000036529. [Q6ZPE2-2]
GeneID77980.
KEGGmmu:77980.
UCSCuc007xfz.1. mouse. [Q6ZPE2-1]
uc011zxu.1. mouse.

Organism-specific databases

CTD6305.
MGIMGI:1925230. Sbf1.
RougeSearch...

Phylogenomic databases

eggNOGNOG315534.
GeneTreeENSGT00740000115188.
HOGENOMHOG000044360.
HOVERGENHBG052527.
InParanoidQ6ZPE2.
OMASEWLIQI.
OrthoDBEOG747PKH.
TreeFamTF318583.

Gene expression databases

ArrayExpressQ6ZPE2.
BgeeQ6ZPE2.
CleanExMM_SBF1.
GenevestigatorQ6ZPE2.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR004182. GRAM.
IPR010569. Myotubularin-like_Pase_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR022096. SBF2.
IPR005113. uDENN_dom.
[Graphical view]
PfamPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF02893. GRAM. 1 hit.
PF06602. Myotub-related. 1 hit.
PF00169. PH. 1 hit.
PF12335. SBF2. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00568. GRAM. 1 hit.
SM00233. PH. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51339. PPASE_MYOTUBULARIN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSBF1. mouse.
EvolutionaryTraceQ6ZPE2.
NextBio347929.
PROQ6ZPE2.
SOURCESearch...

Entry information

Entry nameMTMR5_MOUSE
AccessionPrimary (citable) accession number: Q6ZPE2
Secondary accession number(s): B2RXQ1 expand/collapse secondary AC list , B2RXX4, B7ZWK2, Q4QQM2, Q8BK68, Q8K2Z0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot