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Q6ZNW5 (GDPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GDP-D-glucose phosphorylase 1

EC=2.7.7.78
Gene names
Name:GDPGP1
Synonyms:C15orf58
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells. Ref.3

Catalytic activity

GDP-alpha-D-glucose + phosphate = alpha-D-glucose 1-phosphate + GDP. Ref.3

Subcellular location

Cytoplasm By similarity.

Miscellaneous

The orthologs in A.thaliana are GDP-L-galactose phosphorylases catalyzing the first reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants.

Sequence similarities

Belongs to the GDPGP1 family.

Biophysicochemical properties

Kinetic parameters:

kcat is 26 s(-1) at 31 degrees Celsius. The catalytic efficiency for GDP-D-glucose is 35-fold higher than for GDP-L-galactose.

KM=2 µM for GDP-D-glucose (at 31 degrees Celsius) Ref.3

KM=12 µM for GDP-L-galactose (at 31 degrees Celsius)

KM=42 µM for GDP-D-mannose (at 31 degrees Celsius)

KM=2.9 mM for inorganic phosphate (at 31 degrees Celsius)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385GDP-D-glucose phosphorylase 1
PRO_0000336750

Sites

Active site2181Tele-GMP-histidine intermediate By similarity

Natural variations

Natural variant371M → T. Ref.1
Corresponds to variant rs7171194 [ dbSNP | Ensembl ].
VAR_043555
Natural variant2641P → T. Ref.1
Corresponds to variant rs10152994 [ dbSNP | Ensembl ].
VAR_043556
Natural variant3071T → I. Ref.1
Corresponds to variant rs10153004 [ dbSNP | Ensembl ].
VAR_043557

Experimental info

Sequence conflict231R → G in BAC85370. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6ZNW5 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 921A656C721876EC

FASTA38542,362
        10         20         30         40         50         60 
MALPHDSNET SYLLPPNNED WGRQTIPDFV YGQKDLMAEG IQWPRNAPGI PDALPQSPFD 

        70         80         90        100        110        120 
AALCSAWKQR VELGLFRYRL RELQTQILPG AVGFVAQLNV ERGVQRRPPQ TIKSVRQAFD 

       130        140        150        160        170        180 
PVQFNFNKIR PGEVLFRLHR EPDLPGTLLQ EDILVVINVS PLEWGHVLLV PEPARQLPQR 

       190        200        210        220        230        240 
LLPGALRAGI EAVLLSLHPG FRVGFNSLGG LASVNHLHLH GYYLAHRLPV EQAPSEPLDP 

       250        260        270        280        290        300 
GGHLHLLQDL PAPGFLFYTR GPGPDLESLI SRVCRATDYL TDHEIAHNLF VTRGAPPGKT 

       310        320        330        340        350        360 
SPSSALTGVR VILWARKSSF GIKDGEAFNV ALCELAGHLP VKTSQDFSSL TEAAAVALIQ 

       370        380 
DCRLPPSQAE DVQAALVALM SQEEQ 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS THR-37; THR-264 AND ILE-307.
Tissue: Salivary gland.
[2]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"A novel GDP-D-glucose phosphorylase involved in quality control of the nucleoside diphosphate sugar pool in Caenorhabditis elegans and mammals."
Adler L.N., Gomez T.A., Clarke S.G., Linster C.L.
J. Biol. Chem. 286:21511-21523(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK130527 mRNA. Translation: BAC85370.1.
AC091167 Genomic DNA. No translation available.
CCDSCCDS32327.1.
RefSeqNP_001013679.2. NM_001013657.2.
UniGeneHs.304673.

3D structure databases

ProteinModelPortalQ6ZNW5.
SMRQ6ZNW5. Positions 150-222.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid133642. 3 interactions.
STRING9606.ENSP00000368405.

Polymorphism databases

DMDM296434456.

Proteomic databases

MaxQBQ6ZNW5.
PaxDbQ6ZNW5.
PRIDEQ6ZNW5.

Protocols and materials databases

DNASU390637.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000329600; ENSP00000368405; ENSG00000183208.
ENST00000558017; ENSP00000452793; ENSG00000183208.
GeneID390637.
KEGGhsa:390637.
UCSCuc002bpc.3. human.

Organism-specific databases

CTD390637.
GeneCardsGC15P090778.
HGNCHGNC:34360. GDPGP1.
HPAHPA048189.
neXtProtNX_Q6ZNW5.
PharmGKBPA162378247.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307761.
HOVERGENHBG106673.
InParanoidQ6ZNW5.
KOK15630.
OMAGIQWPRN.
OrthoDBEOG7M98H0.
PhylomeDBQ6ZNW5.
TreeFamTF313615.

Enzyme and pathway databases

BioCycMetaCyc:G66-31756-MONOMER.

Gene expression databases

ArrayExpressQ6ZNW5.
BgeeQ6ZNW5.
CleanExHS_C15orf58.
GenevestigatorQ6ZNW5.

Family and domain databases

InterProIPR026506. GDPGP.
[Graphical view]
PANTHERPTHR20884. PTHR20884. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi390637.
NextBio103948.
PROQ6ZNW5.

Entry information

Entry nameGDPP1_HUMAN
AccessionPrimary (citable) accession number: Q6ZNW5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM