Reviewed, UniProtKB/Swiss-Prot Q6ZNL6 (FGD5_HUMAN)

Names and origin

Protein names

Recommended name:
    FYVE, RhoGEF and PH domain-containing protein 5
Alternative name(s):
    Zinc finger FYVE domain-containing protein 23

Gene names

Name:	FGD5
Synonyms:	ZFYVE23

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape By similarity.
Subcellular location	Cytoplasm Probable. Cytoplasm › cytoskeleton Probable.
Sequence similarities	Contains 1 DH (DBL-homology) domain. Contains 1 FYVE-type zinc finger. Contains 2 PH domains.
Sequence caution	The sequence BAC85128.1 differs from that shown. Reason: Frameshift at position 79. The sequence CAD98090.1 differs from that shown. Reason: Erroneous termination at position 1279. Translated as Lys.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB filopodium assembly Inferred from sequence or structural similarity. Source: UniProtKB regulation of Cdc42 GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell shape Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell lamellipodium Inferred from sequence or structural similarity. Source: UniProtKB ruffle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro small GTPase binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ZNL6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZNL6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-922: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1462	1462	FYVE, RhoGEF and PH domain-containing protein 5		PRO_0000080950
Regions
	Domain	892 – 1084	193	DH
	Domain	1113 – 1207	95	PH 1
	Domain	1363 – 1461	99	PH 2
	Zinc finger	1242 – 1301	60	FYVE-type
	Compositional bias	58 – 323	266	Glu-rich
	Compositional bias	645 – 650	6	Poly-Lys
Amino acid modifications
	Modified residue	632	1	Phosphoserine By similarity
	Modified residue	635	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 922	922	Missing in isoform 2.		VSP_013088
Experimental info
	Sequence conflict	354	1	S → G in CAE45896. Ref.4
	Sequence conflict	1112	1	R → M in CAE45896. Ref.4
	Sequence conflict	1196	1	D → N in CAE45896. Ref.4
	Sequence conflict	1289	1	M → T in CAE45896. Ref.4
	Sequence conflict	1382	1	K → E in BAC04989. Ref.1
	Sequence conflict	1400	1	D → G in CAD98090. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 9ED4C4FC945DE438	FASTA	1,462	159,907
	10 20 30 40 50 60 MFRGPKPPIA PKPRLTAPNE WRASVYLNDS LNKCSNGRLP CVDRGLDEGP RSIPKCSESE 70 80 90 100 110 120 TDEDYIVVPR VPLREDEPKD EGSVGNKALV SPESSAEEEE EREEGGEACG LEGTGAGEDS 130 140 150 160 170 180 VAPTAPGAGA LSREGEEGTD LALEDEGEGC ADEPGTLEQV SRSEEEEKLV QPHRECSLED 190 200 210 220 230 240 SGPWAGEGVF QSDLLLPHIH GEDQEPPDTP GEGEEDDEEG CASTDPAGAD EGSGPDRPTE 250 260 270 280 290 300 DMGQDAEDTS EEPPEKEELA GVQEAETATD CPEVLEEGCE EATGVTGGEQ VDLSEPPDHE 310 320 330 340 350 360 KKTNQEVAAA TLEDHAQDES AEESCQIVPF ENDCMEDFVT SLTGSPYEFF PTESTSFCSE 370 380 390 400 410 420 SCSPLSESAK GLESEQAPKL GLRAEENPMV GALCGQCGSL QGGAAEGPAA PDVVVVLEEE 430 440 450 460 470 480 ALDDALANPY VMGVGLPGQA APGEGGQAAS DALGGYGSKE ELNCEAEGGL VPADRKNTST 490 500 510 520 530 540 RVRPHSGKVA GYVPETVPEE TGPEAGSSAP GIGGAAEEVG KTLLSLEGKP LEASRALPAK 550 560 570 580 590 600 PRAFTLYPRS FSVEGREIPV SVYQEPEGSG LDDHRIKRKE DNLSLSCVIG SSGSFSQRNH 610 620 630 640 650 660 LPSSGTSTPS SMVDIPPPFD LACITKKPIT KSSPSLLIES DSPDKYKKKK SSFKRFLALT 670 680 690 700 710 720 FKKKTENKLH VDVNVSSSRS SSESSYHGPS RILEVDRRSL SNSPQLKSRT GKLRASESPS 730 740 750 760 770 780 SLIFYRDGKR KGVPFSRTVS RVESFEDRSR PPFLPLPLTK PRSISFPSAD TSDYENIPAM 790 800 810 820 830 840 NSDYENIQIP PRRPARAGAF TKLFEDQSRA LSTANENDGY VDMSSFNAFE SKQQSADQDA 850 860 870 880 890 900 ESAYTEPYKV CPISSAAPKE DLTSDEEQRS SEEEDSASRD PSVTHKVEGQ SRALVIAQEL 910 920 930 940 950 960 LSSEKAYVEM LQHLNLDFHG AVMRALDDMD HEGRDTLARE ELRQGLSELP AIHDLHQGIL 970 980 990 1000 1010 1020 EELEERLSNW ESQQKVADVF LAREQGFDHH ATHILQFDRY LGLLSENCLH SPRLAAAVRE 1030 1040 1050 1060 1070 1080 FEQSVQGGSQ TAKHRLLRVV QRLFQYQVLL TDYLNNLCPD SAEYDNTQGA LSLISKVTDR 1090 1100 1110 1120 1130 1140 ANDSMEQGEN LQKLVHIEHS VRGQGDLLQP GREFLKEGTL MKVTGKNRRP RHLFLMNDVL 1150 1160 1170 1180 1190 1200 LYTYPQKDGK YRLKNTLAVA NMKVSRPVME KVPYALKIET SESCLMLSAS SCAERDEWYG 1210 1220 1230 1240 1250 1260 CLSRALPEDY KAQALAAFHH SVEIRERLGV SLGERPPTLV PVTHVMMCMN CGCDFSLTLR 1270 1280 1290 1300 1310 1320 RHHCHACGKI VCRNCSRNKY PLKYLKDRMA KVCDGCFGEL KKRGRAVPGL MRERPVSMSF 1330 1340 1350 1360 1370 1380 PLSSPRFSGS AFSSVFQSIN PSTFKKQKKV PSALTEVAAS GEGSAISGYL SRCKRGKRHW 1390 1400 1410 1420 1430 1440 KKLWFVIKGK VLYTYMASED KVALESMPLL GFTIAPEKEE GSSEVGPIFH LYHKKTLFYS 1450 1460 FKAEDTNSAQ RWIEAMEDAS VL
	Isoform 2. Checksum: A8ED7C2316625D0E	FASTA	540	61,238
	10 20 30 40 50 60 MRALDDMDHE GRDTLAREEL RQGLSELPAI HDLHQGILEE LEERLSNWES QQKVADVFLA 70 80 90 100 110 120 REQGFDHHAT HILQFDRYLG LLSENCLHSP RLAAAVREFE QSVQGGSQTA KHRLLRVVQR 130 140 150 160 170 180 LFQYQVLLTD YLNNLCPDSA EYDNTQGALS LISKVTDRAN DSMEQGENLQ KLVHIEHSVR 190 200 210 220 230 240 GQGDLLQPGR EFLKEGTLMK VTGKNRRPRH LFLMNDVLLY TYPQKDGKYR LKNTLAVANM 250 260 270 280 290 300 KVSRPVMEKV PYALKIETSE SCLMLSASSC AERDEWYGCL SRALPEDYKA QALAAFHHSV 310 320 330 340 350 360 EIRERLGVSL GERPPTLVPV THVMMCMNCG CDFSLTLRRH HCHACGKIVC RNCSRNKYPL 370 380 390 400 410 420 KYLKDRMAKV CDGCFGELKK RGRAVPGLMR ERPVSMSFPL SSPRFSGSAF SSVFQSINPS 430 440 450 460 470 480 TFKKQKKVPS ALTEVAASGE GSAISGYLSR CKRGKRHWKK LWFVIKGKVL YTYMASEDKV 490 500 510 520 530 540 ALESMPLLGF TIAPEKEEGS SEVGPIFHLY HKKTLFYSFK AEDTNSAQRW IEAMEDASVL

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 77-1462 (ISOFORM 1). Tissue: Spleen and Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Shotgun sequencing of the human transcriptome with ORF expressed sequence tags." Dias Neto E., Correa R.G., Verjovski-Almeida S., Briones M.R.S., Nagai M.A., da Silva W. Jr., Zago M.A., Bordin S., Costa F.F., Goldman G.H., Carvalho A.F., Matsukuma A., Baia G.S., Simpson D.H., Brunstein A., de Oliveira P.S.L., Bucher P., Jongeneel C.V. , O'Hare M.J., Soares F., Brentani R.R., Reis L.F.L., de Souza S.J., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 97:3491-3496(2000) [PubMed: 10737800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-215 (ISOFORM 1). Tissue: Placenta.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-1462 (ISOFORM 1). Tissue: Oesophageal carcinoma and Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK096856 mRNA. Translation: BAC04878.1. Different initiation. AK097276 mRNA. Translation: BAC04989.1. Different initiation. AK131078 mRNA. Translation: BAC85128.1. Frameshift. AK123054 mRNA. Translation: BAG53865.1. BC035364 mRNA. Translation: AAH35364.1. BF989107 mRNA. No translation available. BX640820 Transcribed RNA. Translation: CAE45896.2. BX538312 mRNA. Translation: CAD98090.1. Sequence problems.
IPI	IPI00397955. IPI00554548.
UniGene	Hs.412406
3D structure databases
HSSP	HSSP built from PDB template 1HYJ based on UniProtKB Q15075.
ModBase	Search...
PTM databases
PhosphoSite	Q6ZNL6.
Proteomic databases
PRIDE	Q6ZNL6.
Genome annotation databases
Ensembl	ENSG00000154783. Homo sapiens. [Contig view]
Organism-specific databases
GeneCards	GC03P014826.
HGNC	HGNC:19117. FGD5.
PharmGKB	PA134874843.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6ZNL6.
HOVERGEN	Q6ZNL6.
Gene expression databases
ArrayExpress	Q6ZNL6.
Bgee	Q6ZNL6.
CleanEx	HS_FGD5.
GermOnline	ENSG00000154783. Homo sapiens.
Family and domain databases
InterPro	IPR000219. DH-domain. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 2 hits. G3DSA:1.20.900.10. RhoGEF. 1 hit.
Pfam	PF01363. FYVE. 1 hit. PF00169. PH. 2 hits. PF00621. RhoGEF. 1 hit. [Graphical view]
ProDom	PD040407. Peptidase_S48. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00064. FYVE. 1 hit. SM00233. PH. 2 hits. SM00325. RhoGEF. 1 hit. [Graphical view]
PROSITE	PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 2 hits. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	86934.

Entry information

Entry name

FGD5_HUMAN

Accession

Primary (citable) accession number: Q6ZNL6
Secondary accession number(s): B3KVQ3 , Q6MZY1, Q7Z303, Q8IYP3, Q8N861, Q8N8G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)