Q6ZNA4 (RN111_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase Arkadia
EC=6.3.2.-

Alternative name(s):
RING finger protein 111

Gene names

Name:

RNF111

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	994 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation. Ref.6 Ref.7 Ref.8
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7. Interacts (via SIM domains) with SUMO1 and SUMO2. Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus. Cytoplasm. Note: Upon TGF-beta treatment, translocates from nucleus to cytosol. Ref.7
Tissue specificity	Broadly expressed. Ref.6
Sequence similarities	Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAH10369.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC04531.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAD18633.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pattern specification process Inferred from electronic annotation. Source: Compara positive regulation of protein ubiquitination Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: Reactome positive regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara protein polyubiquitination Inferred from electronic annotation. Source: Compara transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transforming growth factor beta receptor signaling pathway Traceable author statement. Source: Reactome ubiquitin-dependent SMAD protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA nucleoplasm Traceable author statement. Source: Reactome protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	SUMO polymer binding Inferred from direct assay Ref.9. Source: UniProtKB ubiquitin-protein ligase activity Inferred from mutant phenotype Ref.7. Source: BHF-UCL zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ZNA4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZNA4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     914-921: Missing.

Isoform 3 (identifier: Q6ZNA4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     766-766: T → TGLFVFCVSR

Note: No experimental confirmation available.

Isoform 4 (identifier: Q6ZNA4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     766-766: T → TGLFVFCVSR
     914-921: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 994

994

E3 ubiquitin-protein ligase Arkadia

PRO_0000280690

Regions

Zinc finger

942 – 983

42

RING-type; atypical

Region

241 – 404

164

Interaction with AXIN1

Motif

300 – 304

5

SUMO interaction motif 1 (SIM); mediates the binding to polysumoylated substrates

Motif

325 – 331

7

SUMO interaction motif 2 (SIM); mediates the binding to polysumoylated substrates

Motif

382 – 386

5

SUMO interaction motif 3 (SIM); mediates the binding to polysumoylated substrates

Compositional bias

252 – 464

213

Ser-rich

Compositional bias

494 – 523

30

His-rich

Compositional bias

629 – 781

153

Pro-rich

Natural variations

Alternative sequence

766

1

T → TGLFVFCVSR in isoform 3 and isoform 4.

VSP_023840

Alternative sequence

914 – 921

8

Missing in isoform 2 and isoform 4.

VSP_023841

Natural variant

9

1

N → K. Ref.1 Ref.2 Ref.5
Corresponds to variant rs2899642 [ dbSNP | Ensembl ].

VAR_031185

Natural variant

718

1

A → T.
Corresponds to variant rs34086812 [ dbSNP | Ensembl ].

VAR_057216

Experimental info

Mutagenesis

382

1

V → A: Abolishes SUMO binding. Ref.9

Mutagenesis

382

1

V → L: Loss of affinity to SUMO1 and SUMO2. Ref.9

Mutagenesis

382

1

V → Y: No loss of affinity toward SUMO1 and SUMO2. Ref.9

Mutagenesis

383

1

V → A: Abolishes SUMO binding. Ref.9

Mutagenesis

383

1

V → I: No loss of affinity toward SUMO1 and SUMO2. Ref.9

Mutagenesis

384

1

D → A: Abolishes SUMO binding. Ref.9

Mutagenesis

384

1

D → E: Loss of affinity to SUMO1 and SUMO2. Ref.9

Mutagenesis

384

1

D → N: Loss of affinity to SUMO1 and SUMO2. Ref.9

Mutagenesis

385

1

L → A: Abolishes SUMO binding. Ref.9

Mutagenesis

385

1

L → I: Loss of affinity to SUMO1 and SUMO2. Ref.9

Mutagenesis

386

1

T → A: Abolishes SUMO binding. Ref.9

Mutagenesis

386

1

T → S: Loss of affinity to SUMO1 and SUMO2. Ref.9

Mutagenesis

386

1

T → V: Loss of affinity to SUMO1 and SUMO2. Ref.9

Sequence conflict

165

1

H → R in BX647259. Ref.2

Sequence conflict

473

1

A → E in CAD98031. Ref.2

Sequence conflict

562

1

Missing in AAH60862. Ref.3

Sequence conflict

562

1

Missing in AAH20984. Ref.3

Sequence conflict

625

1

M → T in BX647259. Ref.2

Sequence conflict

738

1

T → I in BX647259. Ref.2

Sequence conflict

809

1

E → G in CAD98031. Ref.2

Sequence conflict

931

1

E → G in CAD98031. Ref.2

Sequence conflict

938

1

T → A in BX647259. Ref.2

Sequence conflict

974

1

I → V in BAD18471. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

994

Helix Strand Turn

Details...

Turn

943 – 946

4

Beta strand

951 – 953

3

Beta strand

955 – 957

3

Beta strand

963 – 965

3

Helix

966 – 975

10

Turn

980 – 982

3

Beta strand

984 – 986

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 7E387321E5F61FAB	FASTA	994	108,862
	10 20 30 40 50 60 MSQWTPEYNE LYTLKVDMKS EIPSDAPKTQ ESLKGILLHP EPIGAAKSFP AGVEMINSKV 70 80 90 100 110 120 GNEFSHLCDD SQKQEKEMNG NQQEQEKSLV VRKKRKSQQA GPSYVQNCVK ENQGILGLRQ 130 140 150 160 170 180 HLGTPSDEDN DSSFSDCLSS PSSSLHFGDS DTVTSDEDKE VSVRHSQTIL NAKSRSHSAR 190 200 210 220 230 240 SHKWPRTETE SVSGLLMKRP CLHGSSLRRL PCRKRFVKNN SSQRTQKQKE RILMQRKKRE 250 260 270 280 290 300 VLARRKYALL PSSSSSSEND LSSESSSSSS TEGEEDLFVS ASENHQNNPA VPSGSIDEDV 310 320 330 340 350 360 VVIEASSTPQ VTANEEINVT STDSEVEIVT VGESYRSRST LGHSRSHWSQ GSSSHASRPQ 370 380 390 400 410 420 EPRNRSRIST VIQPLRQNAA EVVDLTVDED EPTVVPTTSA RMESQATSAS INNSNPSTSE 430 440 450 460 470 480 QASDTASAVT SSQPSTVSET SATLTSNSTT GTSIGDDSRR TTSSAVTETG PPAMPRLPSC 490 500 510 520 530 540 CPQHSPCGGS SQNHHALGHP HTSCFQQHGH HFQHHHHHHH TPHPAVPVSP SFSDPACPVE 550 560 570 580 590 600 RPPQVQAPCG ANSSSGTSYH EQQALPVDLS NSGIRSHGSG SFHGASAFDP CCPVSSSRAA 610 620 630 640 650 660 IFGHQAAAAA PSQPLSSIDG YGSSMVAQPQ PQPPPQPSLS SCRHYMPPPY ASLTRPLHHQ 670 680 690 700 710 720 ASACPHSHGN PPPQTQPPPQ VDYVIPHPVH AFHSQISSHA TSHPVAPPPP THLASTAAPI 730 740 750 760 770 780 PQHLPPTHQP ISHHIPATAP PAQRLHPHEV MQRMEVQRRR MMQHPTRAHE RPPPHPHRMH 790 800 810 820 830 840 PNYGHGHHIH VPQTMSSHPR QAPERSAWEL GIEAGVTAAT YTPGALHPHL AHYHAPPRLH 850 860 870 880 890 900 HLQLGALPLM VPDMAGYPHI RYISSGLDGT SFRGPFRGNF EELIHLEERL GNVNRGASQG 910 920 930 940 950 960 TIERCTYPHK YKKVTTDWFS QRKLHCKQDG EEGTEEDTEE KCTICLSILE EGEDVRRLPC 970 980 990 MHLFHQVCVD QWLITNKKCP ICRVDIEAQL PSES
	Isoform 2 [UniParc]. Checksum: BD07D5D2C2B6AEB4	FASTA	986	107,896
	10 20 30 40 50 60 MSQWTPEYNE LYTLKVDMKS EIPSDAPKTQ ESLKGILLHP EPIGAAKSFP AGVEMINSKV 70 80 90 100 110 120 GNEFSHLCDD SQKQEKEMNG NQQEQEKSLV VRKKRKSQQA GPSYVQNCVK ENQGILGLRQ 130 140 150 160 170 180 HLGTPSDEDN DSSFSDCLSS PSSSLHFGDS DTVTSDEDKE VSVRHSQTIL NAKSRSHSAR 190 200 210 220 230 240 SHKWPRTETE SVSGLLMKRP CLHGSSLRRL PCRKRFVKNN SSQRTQKQKE RILMQRKKRE 250 260 270 280 290 300 VLARRKYALL PSSSSSSEND LSSESSSSSS TEGEEDLFVS ASENHQNNPA VPSGSIDEDV 310 320 330 340 350 360 VVIEASSTPQ VTANEEINVT STDSEVEIVT VGESYRSRST LGHSRSHWSQ GSSSHASRPQ 370 380 390 400 410 420 EPRNRSRIST VIQPLRQNAA EVVDLTVDED EPTVVPTTSA RMESQATSAS INNSNPSTSE 430 440 450 460 470 480 QASDTASAVT SSQPSTVSET SATLTSNSTT GTSIGDDSRR TTSSAVTETG PPAMPRLPSC 490 500 510 520 530 540 CPQHSPCGGS SQNHHALGHP HTSCFQQHGH HFQHHHHHHH TPHPAVPVSP SFSDPACPVE 550 560 570 580 590 600 RPPQVQAPCG ANSSSGTSYH EQQALPVDLS NSGIRSHGSG SFHGASAFDP CCPVSSSRAA 610 620 630 640 650 660 IFGHQAAAAA PSQPLSSIDG YGSSMVAQPQ PQPPPQPSLS SCRHYMPPPY ASLTRPLHHQ 670 680 690 700 710 720 ASACPHSHGN PPPQTQPPPQ VDYVIPHPVH AFHSQISSHA TSHPVAPPPP THLASTAAPI 730 740 750 760 770 780 PQHLPPTHQP ISHHIPATAP PAQRLHPHEV MQRMEVQRRR MMQHPTRAHE RPPPHPHRMH 790 800 810 820 830 840 PNYGHGHHIH VPQTMSSHPR QAPERSAWEL GIEAGVTAAT YTPGALHPHL AHYHAPPRLH 850 860 870 880 890 900 HLQLGALPLM VPDMAGYPHI RYISSGLDGT SFRGPFRGNF EELIHLEERL GNVNRGASQG 910 920 930 940 950 960 TIERCTYPHK YKKRKLHCKQ DGEEGTEEDT EEKCTICLSI LEEGEDVRRL PCMHLFHQVC 970 980 VDQWLITNKK CPICRVDIEA QLPSES
	Isoform 3 [UniParc]. Checksum: 9F0D0B05B5EB39A8	FASTA	1,003	109,871
	10 20 30 40 50 60 MSQWTPEYNE LYTLKVDMKS EIPSDAPKTQ ESLKGILLHP EPIGAAKSFP AGVEMINSKV 70 80 90 100 110 120 GNEFSHLCDD SQKQEKEMNG NQQEQEKSLV VRKKRKSQQA GPSYVQNCVK ENQGILGLRQ 130 140 150 160 170 180 HLGTPSDEDN DSSFSDCLSS PSSSLHFGDS DTVTSDEDKE VSVRHSQTIL NAKSRSHSAR 190 200 210 220 230 240 SHKWPRTETE SVSGLLMKRP CLHGSSLRRL PCRKRFVKNN SSQRTQKQKE RILMQRKKRE 250 260 270 280 290 300 VLARRKYALL PSSSSSSEND LSSESSSSSS TEGEEDLFVS ASENHQNNPA VPSGSIDEDV 310 320 330 340 350 360 VVIEASSTPQ VTANEEINVT STDSEVEIVT VGESYRSRST LGHSRSHWSQ GSSSHASRPQ 370 380 390 400 410 420 EPRNRSRIST VIQPLRQNAA EVVDLTVDED EPTVVPTTSA RMESQATSAS INNSNPSTSE 430 440 450 460 470 480 QASDTASAVT SSQPSTVSET SATLTSNSTT GTSIGDDSRR TTSSAVTETG PPAMPRLPSC 490 500 510 520 530 540 CPQHSPCGGS SQNHHALGHP HTSCFQQHGH HFQHHHHHHH TPHPAVPVSP SFSDPACPVE 550 560 570 580 590 600 RPPQVQAPCG ANSSSGTSYH EQQALPVDLS NSGIRSHGSG SFHGASAFDP CCPVSSSRAA 610 620 630 640 650 660 IFGHQAAAAA PSQPLSSIDG YGSSMVAQPQ PQPPPQPSLS SCRHYMPPPY ASLTRPLHHQ 670 680 690 700 710 720 ASACPHSHGN PPPQTQPPPQ VDYVIPHPVH AFHSQISSHA TSHPVAPPPP THLASTAAPI 730 740 750 760 770 780 PQHLPPTHQP ISHHIPATAP PAQRLHPHEV MQRMEVQRRR MMQHPTGLFV FCVSRRAHER 790 800 810 820 830 840 PPPHPHRMHP NYGHGHHIHV PQTMSSHPRQ APERSAWELG IEAGVTAATY TPGALHPHLA 850 860 870 880 890 900 HYHAPPRLHH LQLGALPLMV PDMAGYPHIR YISSGLDGTS FRGPFRGNFE ELIHLEERLG 910 920 930 940 950 960 NVNRGASQGT IERCTYPHKY KKVTTDWFSQ RKLHCKQDGE EGTEEDTEEK CTICLSILEE 970 980 990 1000 GEDVRRLPCM HLFHQVCVDQ WLITNKKCPI CRVDIEAQLP SES
	Isoform 4 [UniParc]. Checksum: A648717F19CFB655	FASTA	995	108,906
	10 20 30 40 50 60 MSQWTPEYNE LYTLKVDMKS EIPSDAPKTQ ESLKGILLHP EPIGAAKSFP AGVEMINSKV 70 80 90 100 110 120 GNEFSHLCDD SQKQEKEMNG NQQEQEKSLV VRKKRKSQQA GPSYVQNCVK ENQGILGLRQ 130 140 150 160 170 180 HLGTPSDEDN DSSFSDCLSS PSSSLHFGDS DTVTSDEDKE VSVRHSQTIL NAKSRSHSAR 190 200 210 220 230 240 SHKWPRTETE SVSGLLMKRP CLHGSSLRRL PCRKRFVKNN SSQRTQKQKE RILMQRKKRE 250 260 270 280 290 300 VLARRKYALL PSSSSSSEND LSSESSSSSS TEGEEDLFVS ASENHQNNPA VPSGSIDEDV 310 320 330 340 350 360 VVIEASSTPQ VTANEEINVT STDSEVEIVT VGESYRSRST LGHSRSHWSQ GSSSHASRPQ 370 380 390 400 410 420 EPRNRSRIST VIQPLRQNAA EVVDLTVDED EPTVVPTTSA RMESQATSAS INNSNPSTSE 430 440 450 460 470 480 QASDTASAVT SSQPSTVSET SATLTSNSTT GTSIGDDSRR TTSSAVTETG PPAMPRLPSC 490 500 510 520 530 540 CPQHSPCGGS SQNHHALGHP HTSCFQQHGH HFQHHHHHHH TPHPAVPVSP SFSDPACPVE 550 560 570 580 590 600 RPPQVQAPCG ANSSSGTSYH EQQALPVDLS NSGIRSHGSG SFHGASAFDP CCPVSSSRAA 610 620 630 640 650 660 IFGHQAAAAA PSQPLSSIDG YGSSMVAQPQ PQPPPQPSLS SCRHYMPPPY ASLTRPLHHQ 670 680 690 700 710 720 ASACPHSHGN PPPQTQPPPQ VDYVIPHPVH AFHSQISSHA TSHPVAPPPP THLASTAAPI 730 740 750 760 770 780 PQHLPPTHQP ISHHIPATAP PAQRLHPHEV MQRMEVQRRR MMQHPTGLFV FCVSRRAHER 790 800 810 820 830 840 PPPHPHRMHP NYGHGHHIHV PQTMSSHPRQ APERSAWELG IEAGVTAATY TPGALHPHLA 850 860 870 880 890 900 HYHAPPRLHH LQLGALPLMV PDMAGYPHIR YISSGLDGTS FRGPFRGNFE ELIHLEERLG 910 920 930 940 950 960 NVNRGASQGT IERCTYPHKY KKRKLHCKQD GEEGTEEDTE EKCTICLSIL EEGEDVRRLP 970 980 990 CMHLFHQVCV DQWLITNKKC PICRVDIEAQ LPSES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT LYS-9. Tissue: Thymus and Tongue.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), VARIANT LYS-9. Tissue: Amygdala and Endometrium.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 553-994 (ISOFORM 1), VARIANT LYS-9. Tissue: Brain, Colon and Placenta.
[6]	"Arkadia amplifies TGF-beta superfamily signaling through degradation of Smad7." Koinuma D., Shinozaki M., Komuro A., Goto K., Saitoh M., Hanyu A., Ebina M., Nukiwa T., Miyazawa K., Imamura T., Miyazono K. EMBO J. 22:6458-6470(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia." Liu W., Rui H., Wang J., Lin S., He Y., Chen M., Li Q., Ye Z., Zhang S., Chan S.C., Chen Y.-G., Han J., Lin S.-C. EMBO J. 25:1646-1658(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AXIN1 AND AXIN2, IDENTIFICATION IN COMPLEX WITH AXIN1 AND SMAD7, SUBCELLULAR LOCATION, FUNCTION.
[8]	"Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation." Levy L., Howell M., Das D., Harkin S., Episkopou V., Hill C.S. Mol. Cell. Biol. 27:6068-6083(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKIL, FUNCTION.
[9]	"PolySUMO-binding proteins identified through a string search." Sun H., Hunter T. J. Biol. Chem. 287:42071-42083(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF REPEAT SUMO-INTERACTING MOTIF, INTERACTION WITH SUMO1 AND SUMO2, MUTAGENESIS OF VAL-382; VAL-383; ASP-384; LEU-385 AND THR-386.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK095327 mRNA. Translation: BAC04531.1. Different initiation.
AK131304 mRNA. Translation: BAD18471.1.
AK131488 mRNA. Translation: BAD18633.1. Different initiation.
AL157474 mRNA. Translation: CAB75669.2.
BX538130 mRNA. Translation: CAD98031.1.
BX647259 mRNA. No translation available.
AC025918 Genomic DNA. No translation available.
AC090515 Genomic DNA. No translation available.
AC092757 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77561.1.
BC010369 mRNA. Translation: AAH10369.1. Different initiation.
BC020984 mRNA. Translation: AAH20984.1.
BC060862 mRNA. Translation: AAH60862.1.

IPI

IPI00418593.
IPI00798384.
IPI00829658.

PIR

T46904.

RefSeq

NP_001257457.1. NM_001270528.1.
NP_001257458.1. NM_001270529.1.
NP_001257459.1. NM_001270530.1.
NP_060080.6. NM_017610.7.

UniGene

Hs.404423.
Hs.603489.
Hs.741727.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KIZ	NMR	-	A	927-994	[»]

ProteinModelPortal

Q6ZNA4.

SMR

Q6ZNA4. Positions 941-994.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6ZNA4. 29 interactions.

MINT

MINT-1187466.

STRING

9606.ENSP00000288199.

PTM databases

PhosphoSite

Q6ZNA4.

Polymorphism databases

DMDM

308153555.

Proteomic databases

PaxDb

Q6ZNA4.

PRIDE

Q6ZNA4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000348370; ENSP00000288199; ENSG00000157450.
ENST00000434298; ENSP00000393641; ENSG00000157450.
ENST00000557998; ENSP00000452732; ENSG00000157450.
ENST00000559209; ENSP00000453872; ENSG00000157450.
ENST00000561186; ENSP00000453015; ENSG00000157450.

GeneID

54778.

KEGG

hsa:54778.

UCSC

uc002afs.3. human.
uc002aft.3. human.
uc002afv.3. human.

Organism-specific databases

CTD

54778.

GeneCards

GC15P059160.

HGNC

HGNC:17384. RNF111.

HPA

HPA038576.
HPA038577.

MIM

605840. gene.

neXtProt

NX_Q6ZNA4.

PharmGKB

PA134868772.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG291583.

HOVERGEN

HBG093884.

InParanoid

Q6ZNA4.

KO

K10635.

OMA

CDDSQKQ.

Enzyme and pathway databases

Pathway_Interaction_DB

tgfbrpathway. TGF-beta receptor signaling.

Reactome

REACT_111102. Signal Transduction.
REACT_6900. Immune System.
REACT_71. Gene Expression.

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

Q6ZNA4.

Bgee

Q6ZNA4.

CleanEx

HS_RNF111.

Genevestigator

Q6ZNA4.

Family and domain databases

Gene3D

3.30.40.10. 1 hit.

InterPro

IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Pfam

PF13639. zf-RING_2. 1 hit.
[Graphical view]

SMART

SM00184. RING. 1 hit.
[Graphical view]

PROSITE

PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

54778.

NextBio

57437.

SOURCE

Search...

Entry information

Entry name

RN111_HUMAN

Accession

Primary (citable) accession number: Q6ZNA4
Secondary accession number(s): C9JUS4 , H0YN55, Q6P9A4, Q6ZMU2, Q7L428, Q7Z346, Q8N1P9, Q8WUA3, Q9NSR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.