Q6ZN30 (BNC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein basonuclin-2

Gene names

Name:

BNC2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1099 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor specific for skin keratinocytes. May play a role in the differentiation of spermatozoa and oocytes. Ref.4
Subcellular location	Nucleus Ref.3.
Tissue specificity	Highly expressed in testis, uterus and small intestine, and weakly expressed in colon and prostate. Also expressed in skin, primary keratinocytes, immortalized keratinocytes, and HeLa and HEK293 cells. Not detected in blood, thymus, spleen or Hep-G2 cells. Ref.3 Ref.4
Sequence similarities	Contains 4 C2H2-type zinc fingers.
Sequence caution	The sequence AAR99389.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA90908.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15269.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC03837.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.3 (identifier: Q6ZN30-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ZN30-2)

The sequence of this isoform differs from the canonical sequence as follows:
     881-903: HSANINLHRKLLTKELDDMGLDS → YWEKSNEQNGLLVSWGETLSSLK
     904-1099: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1099	1099	Zinc finger protein basonuclin-2		PRO_0000046934
Regions
	Zinc finger	441 – 464	24	C2H2-type 1
	Zinc finger	833 – 856	24	C2H2-type 2
	Zinc finger	1035 – 1058	24	C2H2-type 3
	Zinc finger	1063 – 1090	28	C2H2-type 4
	Compositional bias	534 – 614	81	Pro-rich
Amino acid modifications
	Modified residue	561	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	881 – 903	23	HSANI…MGLDS → YWEKSNEQNGLLVSWGETLS SLK in isoform 2.		VSP_051873
	Alternative sequence	904 – 1099	196	Missing in isoform 2.		VSP_051874
	Natural variant	550	1	L → V. Corresponds to variant rs4961490 [ dbSNP | Ensembl ].		VAR_052707
	Natural variant	782	1	T → A. Corresponds to variant rs3739714 [ dbSNP | Ensembl ].		VAR_033543
Experimental info
	Sequence conflict	560	1	F → L in BAC03837. Ref.1
	Sequence conflict	630	1	K → T in BAC03837. Ref.1
	Sequence conflict	797	1	G → E in BAB15269. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B1601EACD158D3D3	FASTA	1,099	122,330
	10 20 30 40 50 60 MAHLGPTPPP HSLNYKSEDR LSEQDWPAYF KVPCCGVDTS QIESEEAEVD VRERETQRDR 70 80 90 100 110 120 EPKRARDLTL RDSCTDNSMQ FGTRTTTAEP GFMGTWQNAD TNLLFRMSQQ AIRCTLVNCT 130 140 150 160 170 180 CECFQPGKIN LRTCDQCKHG WVAHALDKLS TQHLYHPTQV EIVQSNVVFD ISSLMLYGTQ 190 200 210 220 230 240 AVPVRLKILL DRLFSVLKQE EVLHILHGLG WTLRDYVRGY ILQDAAGKVL DRWAIMSREE 250 260 270 280 290 300 EIITLQQFLR FGETKSIVEL MAIQEKEGQA VAVPSSKTDS DIRTFIESNN RTRSPSLLAH 310 320 330 340 350 360 LENSNPSSIH HFENIPNSLA FLLPFQYINP VSAPLLGLPP NGLLLEQPGL RLREPSLSTQ 370 380 390 400 410 420 NEYNESSESE VSPTPYKNDQ TPNRNALTSI TNVEPKTEPA CVSPIQNSAP VSDLTKTEHP 430 440 450 460 470 480 KSSFRIHRMR RMGSASRKGR VFCNACGKTF YDKGTLKIHY NAVHLKIKHR CTIEGCNMVF 490 500 510 520 530 540 SSLRSRNRHS ANPNPRLHMP MLRNNRDKDL IRATSGAATP VIASTKSNLA LTSPGRPPMG 550 560 570 580 590 600 FTTPPLDPVL QNPLPSQLVF SGLKTVQPVP PFYRSLLTPG EMVSPPTSLP TSPIIPTSGT 610 620 630 640 650 660 IEQHPPPPSE PVVPAVMMAT HEPSADLAPK KKPRKSSMPV KIEKEIIDTA DEFDDEDDDP 670 680 690 700 710 720 NDGGAVVNDM SHDNHCHSQE EMSPGMSVKD FSKHNRTRCI SRTEIRRADS MTSEDQEPER 730 740 750 760 770 780 DYENESESSE PKLGEESMEG DEHIHSEVSE KVLMNSERPD ENHSEPSHQD VIKVKEEFTD 790 800 810 820 830 840 PTYDMFYMSQ YGLYNGGGAS MAALHESFTS SLNYGSPQKF SPEGDLCSSP DPKICYVCKK 850 860 870 880 890 900 SFKSSYSVKL HYRNVHLKEM HVCTVAGCNA AFPSRRSRDR HSANINLHRK LLTKELDDMG 910 920 930 940 950 960 LDSSQPSLSK DLRDEFLVKI YGAQHPMGLD VREDASSPAG TEDSHLNGYG RGMAEDYMVL 970 980 990 1000 1010 1020 DLSTTSSLQS SSSIHSSRES DAGSDEGILL DDIDGASDSG ESAHKAEAPA LPGSLGAEVS 1030 1040 1050 1060 1070 1080 GSLMFSSLSG SNGGIMCNIC HKMYSNKGTL RVHYKTVHLR EMHKCKVPGC NMMFSSVRSR 1090 NRHSQNPNLH KNIPFTSVD
	Isoform 2 [UniParc]. Checksum: CDE800F776B4644F	FASTA	903	101,333
	10 20 30 40 50 60 MAHLGPTPPP HSLNYKSEDR LSEQDWPAYF KVPCCGVDTS QIESEEAEVD VRERETQRDR 70 80 90 100 110 120 EPKRARDLTL RDSCTDNSMQ FGTRTTTAEP GFMGTWQNAD TNLLFRMSQQ AIRCTLVNCT 130 140 150 160 170 180 CECFQPGKIN LRTCDQCKHG WVAHALDKLS TQHLYHPTQV EIVQSNVVFD ISSLMLYGTQ 190 200 210 220 230 240 AVPVRLKILL DRLFSVLKQE EVLHILHGLG WTLRDYVRGY ILQDAAGKVL DRWAIMSREE 250 260 270 280 290 300 EIITLQQFLR FGETKSIVEL MAIQEKEGQA VAVPSSKTDS DIRTFIESNN RTRSPSLLAH 310 320 330 340 350 360 LENSNPSSIH HFENIPNSLA FLLPFQYINP VSAPLLGLPP NGLLLEQPGL RLREPSLSTQ 370 380 390 400 410 420 NEYNESSESE VSPTPYKNDQ TPNRNALTSI TNVEPKTEPA CVSPIQNSAP VSDLTKTEHP 430 440 450 460 470 480 KSSFRIHRMR RMGSASRKGR VFCNACGKTF YDKGTLKIHY NAVHLKIKHR CTIEGCNMVF 490 500 510 520 530 540 SSLRSRNRHS ANPNPRLHMP MLRNNRDKDL IRATSGAATP VIASTKSNLA LTSPGRPPMG 550 560 570 580 590 600 FTTPPLDPVL QNPLPSQLVF SGLKTVQPVP PFYRSLLTPG EMVSPPTSLP TSPIIPTSGT 610 620 630 640 650 660 IEQHPPPPSE PVVPAVMMAT HEPSADLAPK KKPRKSSMPV KIEKEIIDTA DEFDDEDDDP 670 680 690 700 710 720 NDGGAVVNDM SHDNHCHSQE EMSPGMSVKD FSKHNRTRCI SRTEIRRADS MTSEDQEPER 730 740 750 760 770 780 DYENESESSE PKLGEESMEG DEHIHSEVSE KVLMNSERPD ENHSEPSHQD VIKVKEEFTD 790 800 810 820 830 840 PTYDMFYMSQ YGLYNGGGAS MAALHESFTS SLNYGSPQKF SPEGDLCSSP DPKICYVCKK 850 860 870 880 890 900 SFKSSYSVKL HYRNVHLKEM HVCTVAGCNA AFPSRRSRDR YWEKSNEQNG LLVSWGETLS SLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Colon, Kidney epithelium, Teratocarcinoma and Tongue.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes." Romano R.-A., Li H., Tummala R., Maul R., Sinha S. Genomics 83:821-833(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-1099 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"Basonuclin 2: an extremely conserved homolog of the zinc finger protein basonuclin." Vanhoutteghem A., Djian P. Proc. Natl. Acad. Sci. U.S.A. 101:3468-3473(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROBABLE FUNCTION, TISSUE SPECIFICITY.
[5]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-561, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK000050 mRNA. Translation: BAA90908.1. Different initiation. AK025882 mRNA. Translation: BAB15269.1. Different initiation. AK092247 mRNA. Translation: BAC03837.1. Different initiation. AK131398 mRNA. Translation: BAD18545.1. AL450105, AL449983, AL450003 Genomic DNA. Translation: CAM21221.1. AY438376 mRNA. Translation: AAR99389.1. Different initiation.
IPI	IPI00014826. IPI00465099.
RefSeq	NP_060107.3. NM_017637.5.
UniGene	Hs.656581. Hs.693436. Hs.740070.
3D structure databases
ProteinModelPortal	Q6ZN30.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZN30. 1 interaction.
STRING	9606.ENSP00000370047.
PTM databases
PhosphoSite	Q6ZN30.
Polymorphism databases
DMDM	74762393.
Proteomic databases
PaxDb	Q6ZN30.
PRIDE	Q6ZN30.
Protocols and materials databases
DNASU	54796.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380666; ENSP00000370041; ENSG00000173068. ENST00000380672; ENSP00000370047; ENSG00000173068. ENST00000484726; ENSP00000431516; ENSG00000173068.
GeneID	54796.
KEGG	hsa:54796.
UCSC	uc003zmi.3. human.
Organism-specific databases
CTD	54796.
GeneCards	GC09M016410.
HGNC	HGNC:30988. BNC2.
HPA	HPA018525.
MIM	608669. gene.
neXtProt	NX_Q6ZN30.
PharmGKB	PA134953132.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG82846.
HOGENOM	HOG000049098.
HOVERGEN	HBG050706.
InParanoid	Q6ZN30.
OMA	DQTPNRN.
OrthoDB	EOG4VQ9NQ.
Gene expression databases
ArrayExpress	Q6ZN30.
Bgee	Q6ZN30.
CleanEx	HS_BNC2.
Genevestigator	Q6ZN30.
GermOnline	ENSG00000173068. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
SMART	SM00355. ZnF_C2H2. 6 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BNC2. human.
GenomeRNAi	54796.
NextBio	57475.
SOURCE	Search...

Entry information

Entry name

BNC2_HUMAN

Accession

Primary (citable) accession number: Q6ZN30
Secondary accession number(s): B1APG9 , Q6T3A3, Q8NAR2, Q9H6J0, Q9NXV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.