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Protein

Thrombospondin type-1 domain-containing protein 4

Gene

THSD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin type-1 domain-containing protein 4
Alternative name(s):
A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6
Short name:
ADAMTS-like protein 6
Short name:
ADAMTSL-6
Gene namesi
Name:THSD4
ORF Names:UNQ9334/PRO34005
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:25835. THSD4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • microfibril Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485631.

Polymorphism and mutation databases

BioMutaiTHSD4.
DMDMi166229088.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 1018993Thrombospondin type-1 domain-containing protein 4PRO_0000313583Add
BLAST

Proteomic databases

PaxDbiQ6ZMP0.
PRIDEiQ6ZMP0.

PTM databases

iPTMnetiQ6ZMP0.
PhosphoSiteiQ6ZMP0.

Expressioni

Gene expression databases

BgeeiQ6ZMP0.
CleanExiHS_THSD4.
ExpressionAtlasiQ6ZMP0. baseline and differential.
GenevisibleiQ6ZMP0. HS.

Organism-specific databases

HPAiHPA026845.

Interactioni

Subunit structurei

Interacts with FBN1. May interact with TGFB1.By similarity

Protein-protein interaction databases

BioGridi122963. 19 interactions.
IntActiQ6ZMP0. 1 interaction.
STRINGi9606.ENSP00000261862.

Structurei

3D structure databases

ProteinModelPortaliQ6ZMP0.
SMRiQ6ZMP0. Positions 290-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 307255TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini676 – 73762TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini739 – 79254TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini793 – 85159TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini852 – 91160TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini912 – 96857TSP type-1 6PROSITE-ProRule annotationAdd
BLAST
Domaini971 – 100838PLACPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PLAC domain.PROSITE-ProRule annotation
Contains 6 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
KOG4597. Eukaryota.
ENOG41102GD. LUCA.
GeneTreeiENSGT00760000118885.
HOGENOMiHOG000034174.
HOVERGENiHBG080879.
InParanoidiQ6ZMP0.
OMAiINVTEMV.
OrthoDBiEOG73FQKT.
PhylomeDBiQ6ZMP0.
TreeFamiTF316874.

Family and domain databases

InterProiIPR010294. ADAM_spacer1.
IPR010909. PLAC.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF08686. PLAC. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 7 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 7 hits.
PROSITEiPS50900. PLAC. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZMP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSHFMGSLS VLCFLLLLGF QFVCPQPSTQ HRKVPQRMAA EGAPEDDGGG
60 70 80 90 100
GAPGVWGAWG PWSACSRSCS GGVMEQTRPC LPRSYRLRGG QRPGAPARAF
110 120 130 140 150
ADHVVSAVRT SVPLHRSRDE TPALAGTDAS RQGPTVLRGS RHPQPQGLEV
160 170 180 190 200
TGDRRSRTRG TIGPGKYGYG KAPYILPLQT DTAHTPQRLR RQKLSSRHSR
210 220 230 240 250
SQGASSARHG YSSPAHQVPQ HGPLYQSDSG PRSGLQAAEA PIYQLPLTHD
260 270 280 290 300
QGYPAASSLF HSPETSNNHG VGTHGATQSF SQPARSTAIS CIGAYRQYKL
310 320 330 340 350
CNTNVCPESS RSIREVQCAS YNNKPFMGRF YEWEPFAEVK GNRKCELNCQ
360 370 380 390 400
AMGYRFYVRQ AEKVIDGTPC DQNGTAICVS GQCKSIGCDD YLGSDKVVDK
410 420 430 440 450
CGVCGGDNTG CQVVSGVFKH ALTSLGYHRV VEIPEGATKI NITEMYKSNN
460 470 480 490 500
YLALRSRSGR SIINGNWAID RPGKYEGGGT MFTYKRPNEI SSTAGESFLA
510 520 530 540 550
EGPTNEILDV YMIHQQPNPG VHYEYVIMGT NAISPQVPPH RRPGEPFNGQ
560 570 580 590 600
MVTEGRSQEE GEQKGRNEEK EDLRGEAPEM FTSESAQTFP VRHPDRFSPH
610 620 630 640 650
RPDNLVPPAP QPPRRSRDHN WKQLGTTECS TTCGKGSQYP IFRCVHRSTH
660 670 680 690 700
EEAPESYCDS SMKPTPEEEP CNIFPCPAFW DIGEWSECSK TCGLGMQHRQ
710 720 730 740 750
VLCRQVYANR SLTVQPYRCQ HLEKPETTST CQLKICSEWQ IRTDWTSCSV
760 770 780 790 800
PCGVGQRTRD VKCVSNIGDV VDDEECNMKL RPNDIENCDM GPCAKSWFLT
810 820 830 840 850
EWSERCSAEC GAGVRTRSVV CMTNHVSSLP LEGCGNNRPA EATPCDNGPC
860 870 880 890 900
TGKVEWFAGS WSQCSIECGS GTQQREVICV RKNADTFEVL DPSECSFLEK
910 920 930 940 950
PPSQQSCHLK PCGAKWFSTE WSMCSKSCQG GFRVREVRCL SDDMTLSNLC
960 970 980 990 1000
DPQLKPEERE SCNPQDCVPE VDENCKDKYY NCNVVVQARL CVYNYYKTAC
1010
CASCTRVANR QTGFLGSR
Length:1,018
Mass (Da):112,450
Last modified:January 15, 2008 - v2
Checksum:i67D710FBA3ABAFBC
GO
Isoform 2 (identifier: Q6ZMP0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     512-523: MIHQQPNPGVHY → VSLDVSGLFFGF
     524-1018: Missing.

Show »
Length:523
Mass (Da):56,628
Checksum:i4AD13F0A4E4594EF
GO
Isoform 3 (identifier: Q6ZMP0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-360: Missing.
     361-384: AEKVIDGTPCDQNGTAICVSGQCK → MFVSYLILTLLHVQTAVLARPGGE
     450-579: NYLALRSRSG...KEDLRGEAPE → KKKSHLKPAT...REIQILPGPV
     580-1018: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,246
Checksum:i2EB28120469F0E15
GO
Isoform 4 (identifier: Q6ZMP0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: MVSHFMGSLSVLCFLLLLGFQFVC → MFVSYLILTLLHVQTAVLARPGGE
     25-384: Missing.

Note: No experimental confirmation available.
Show »
Length:658
Mass (Da):73,408
Checksum:i470AE1076A3093F1
GO

Sequence cautioni

The sequence BAB14673.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041V → A in BAD18685 (PubMed:14702039).Curated
Sequence conflicti434 – 4341P → S in BAD18685 (PubMed:14702039).Curated
Sequence conflicti806 – 8061C → S in BAB14673 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 360360Missing in isoform 3. 1 PublicationVSP_030036Add
BLAST
Alternative sequencei1 – 2424MVSHF…FQFVC → MFVSYLILTLLHVQTAVLAR PGGE in isoform 4. 1 PublicationVSP_054877Add
BLAST
Alternative sequencei25 – 384360Missing in isoform 4. 1 PublicationVSP_054878Add
BLAST
Alternative sequencei361 – 38424AEKVI…SGQCK → MFVSYLILTLLHVQTAVLAR PGGE in isoform 3. 1 PublicationVSP_030037Add
BLAST
Alternative sequencei450 – 579130NYLAL…GEAPE → KKKSHLKPATRGSQFSSVKV CSVPAACKLLGTPGRARQPV PAPRELEHDKNSPHCAYLSL YLTSLAQSSWRVFSLFSYVL IYLFSKYLAFNTLFALKRMA LQRDRKEKTRAWCIFIKLCG REIQILPGPV in isoform 3. 1 PublicationVSP_030038Add
BLAST
Alternative sequencei512 – 52312MIHQQ…PGVHY → VSLDVSGLFFGF in isoform 2. 1 PublicationVSP_030039Add
BLAST
Alternative sequencei524 – 1018495Missing in isoform 2. 1 PublicationVSP_030040Add
BLAST
Alternative sequencei580 – 1018439Missing in isoform 3. 1 PublicationVSP_030041Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358143 mRNA. Translation: AAQ88510.1.
AK023772 mRNA. Translation: BAB14673.1. Different initiation.
AK131551 mRNA. Translation: BAD18685.1.
AK299056 mRNA. Translation: BAG61125.1.
AC015711 Genomic DNA. No translation available.
AC026636 Genomic DNA. No translation available.
AC064799 Genomic DNA. No translation available.
AC068181 Genomic DNA. No translation available.
AC108861 Genomic DNA. No translation available.
AC104938 Genomic DNA. No translation available.
AC104943 Genomic DNA. No translation available.
AC105132 Genomic DNA. No translation available.
BC140868 mRNA. Translation: AAI40869.1.
BX641106 mRNA. Translation: CAE46049.1.
CCDSiCCDS10238.2. [Q6ZMP0-1]
CCDS66817.1. [Q6ZMP0-4]
RefSeqiNP_001273358.1. NM_001286429.1. [Q6ZMP0-4]
NP_079093.2. NM_024817.2. [Q6ZMP0-1]
XP_006720755.1. XM_006720692.2. [Q6ZMP0-1]
UniGeneiHs.387057.

Genome annotation databases

EnsembliENST00000261862; ENSP00000261862; ENSG00000187720. [Q6ZMP0-3]
ENST00000355327; ENSP00000347484; ENSG00000187720. [Q6ZMP0-1]
ENST00000357769; ENSP00000350413; ENSG00000187720. [Q6ZMP0-4]
GeneIDi79875.
KEGGihsa:79875.
UCSCiuc002atb.2. human. [Q6ZMP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358143 mRNA. Translation: AAQ88510.1.
AK023772 mRNA. Translation: BAB14673.1. Different initiation.
AK131551 mRNA. Translation: BAD18685.1.
AK299056 mRNA. Translation: BAG61125.1.
AC015711 Genomic DNA. No translation available.
AC026636 Genomic DNA. No translation available.
AC064799 Genomic DNA. No translation available.
AC068181 Genomic DNA. No translation available.
AC108861 Genomic DNA. No translation available.
AC104938 Genomic DNA. No translation available.
AC104943 Genomic DNA. No translation available.
AC105132 Genomic DNA. No translation available.
BC140868 mRNA. Translation: AAI40869.1.
BX641106 mRNA. Translation: CAE46049.1.
CCDSiCCDS10238.2. [Q6ZMP0-1]
CCDS66817.1. [Q6ZMP0-4]
RefSeqiNP_001273358.1. NM_001286429.1. [Q6ZMP0-4]
NP_079093.2. NM_024817.2. [Q6ZMP0-1]
XP_006720755.1. XM_006720692.2. [Q6ZMP0-1]
UniGeneiHs.387057.

3D structure databases

ProteinModelPortaliQ6ZMP0.
SMRiQ6ZMP0. Positions 290-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122963. 19 interactions.
IntActiQ6ZMP0. 1 interaction.
STRINGi9606.ENSP00000261862.

PTM databases

iPTMnetiQ6ZMP0.
PhosphoSiteiQ6ZMP0.

Polymorphism and mutation databases

BioMutaiTHSD4.
DMDMi166229088.

Proteomic databases

PaxDbiQ6ZMP0.
PRIDEiQ6ZMP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261862; ENSP00000261862; ENSG00000187720. [Q6ZMP0-3]
ENST00000355327; ENSP00000347484; ENSG00000187720. [Q6ZMP0-1]
ENST00000357769; ENSP00000350413; ENSG00000187720. [Q6ZMP0-4]
GeneIDi79875.
KEGGihsa:79875.
UCSCiuc002atb.2. human. [Q6ZMP0-1]

Organism-specific databases

CTDi79875.
GeneCardsiTHSD4.
H-InvDBHIX0012400.
HGNCiHGNC:25835. THSD4.
HPAiHPA026845.
MIMi614476. gene.
neXtProtiNX_Q6ZMP0.
PharmGKBiPA143485631.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
KOG4597. Eukaryota.
ENOG41102GD. LUCA.
GeneTreeiENSGT00760000118885.
HOGENOMiHOG000034174.
HOVERGENiHBG080879.
InParanoidiQ6ZMP0.
OMAiINVTEMV.
OrthoDBiEOG73FQKT.
PhylomeDBiQ6ZMP0.
TreeFamiTF316874.

Enzyme and pathway databases

ReactomeiR-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

ChiTaRSiTHSD4. human.
GenomeRNAii79875.
NextBioi35474271.
PROiQ6ZMP0.
SOURCEiSearch...

Gene expression databases

BgeeiQ6ZMP0.
CleanExiHS_THSD4.
ExpressionAtlasiQ6ZMP0. baseline and differential.
GenevisibleiQ6ZMP0. HS.

Family and domain databases

InterProiIPR010294. ADAM_spacer1.
IPR010909. PLAC.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF08686. PLAC. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 7 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 7 hits.
PROSITEiPS50900. PLAC. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Mammary gland and Placenta.
  3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 203-1018 (ISOFORM 2).
    Tissue: Uterus.

Entry informationi

Entry nameiTHSD4_HUMAN
AccessioniPrimary (citable) accession number: Q6ZMP0
Secondary accession number(s): B2RTY3
, B4DR13, Q6MZI3, Q6UXZ8, Q9H8E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: March 16, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.