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Protein

Sialic acid-binding Ig-like lectin 15

Gene

SIGLEC15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds sialylated glycoproteins.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431Sialic acidBy similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-2172127. DAP12 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 15
Short name:
Siglec-15
Alternative name(s):
CD33 antigen-like 3
Gene namesi
Name:SIGLEC15
Synonyms:CD33L3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:27596. SIGLEC15.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 263244ExtracellularSequence analysisAdd
BLAST
Transmembranei264 – 28421HelicalSequence analysisAdd
BLAST
Topological domaini285 – 32844CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi143 – 1431R → A: Abrogates glycan-binding. 1 Publication
Mutagenesisi274 – 2741K → A: Abrogates interaction with HCST and TYROBP. 1 Publication

Organism-specific databases

PharmGKBiPA162403350.

Polymorphism and mutation databases

BioMutaiSIGLEC15.
DMDMi74762385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 328309Sialic acid-binding Ig-like lectin 15PRO_0000294369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 142PROSITE-ProRule annotation
Disulfide bondi95 ↔ 104PROSITE-ProRule annotation
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence analysis
Disulfide bondi187 ↔ 237PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6ZMC9.
PeptideAtlasiQ6ZMC9.
PRIDEiQ6ZMC9.

PTM databases

iPTMnetiQ6ZMC9.
PhosphoSiteiQ6ZMC9.

Expressioni

Tissue specificityi

Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.

Gene expression databases

BgeeiQ6ZMC9.
CleanExiHS_SIGLEC15.
ExpressionAtlasiQ6ZMC9. baseline and differential.

Interactioni

Subunit structurei

Interacts with TYROBP and HCST.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000374125.

Structurei

3D structure databases

ProteinModelPortaliQ6ZMC9.
SMRiQ6ZMC9. Positions 44-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 158119Ig-like V-typeAdd
BLAST
Domaini168 – 25184Ig-like C2-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi276 – 2794Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJR. Eukaryota.
ENOG4111T39. LUCA.
GeneTreeiENSGT00390000011536.
HOGENOMiHOG000013049.
HOVERGENiHBG099550.
InParanoidiQ6ZMC9.
KOiK16352.
OMAiVYLFRFR.
OrthoDBiEOG7TF79D.
PhylomeDBiQ6ZMC9.
TreeFamiTF351096.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZMC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKSIWLLAC LAWVLPTGSF VRTKIDTTEN LLNTEVHSSP AQRWSMQVPP
60 70 80 90 100
EVSAEAGDAA VLPCTFTHPH RHYDGPLTAI WRAGEPYAGP QVFRCAAARG
110 120 130 140 150
SELCQTALSL HGRFRLLGNP RRNDLSLRVE RLALADDRRY FCRVEFAGDV
160 170 180 190 200
HDRYESRHGV RLHVTAAPRI VNISVLPSPA HAFRALCTAE GEPPPALAWS
210 220 230 240 250
GPALGNSLAA VRSPREGHGH LVTAELPALT HDGRYTCTAA NSLGRSEASV
260 270 280 290 300
YLFRFHGASG ASTVALLLGA LGFKALLLLG VLAARAARRR PEHLDTPDTP
310 320
PRSQAQESNY ENLSQMNPRS PPATMCSP
Length:328
Mass (Da):35,653
Last modified:July 5, 2004 - v1
Checksum:i534D6D2E76F04C8B
GO
Isoform 2 (identifier: Q6ZMC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MEKSIWLLACLA → MTATRAATASGS
     13-166: Missing.

Note: No experimental confirmation available.
Show »
Length:174
Mass (Da):18,086
Checksum:i2B62AB00ADE77B9B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti273 – 2731F → L.
Corresponds to variant rs2919643 [ dbSNP | Ensembl ].
VAR_033174

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212MEKSI…LACLA → MTATRAATASGS in isoform 2. 1 PublicationVSP_056817Add
BLAST
Alternative sequencei13 – 166154Missing in isoform 2. 1 PublicationVSP_056818Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172835 mRNA. Translation: BAD18800.1.
AK290400 mRNA. Translation: BAF83089.1.
AK290402 mRNA. Translation: BAF83091.1.
AK301189 mRNA. Translation: BAG62771.1.
AC087685 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01463.1.
CCDSiCCDS32819.1. [Q6ZMC9-1]
RefSeqiNP_998767.1. NM_213602.2. [Q6ZMC9-1]
UniGeneiHs.287692.

Genome annotation databases

EnsembliENST00000389474; ENSP00000374125; ENSG00000197046. [Q6ZMC9-1]
ENST00000546268; ENSP00000443509; ENSG00000197046. [Q6ZMC9-2]
GeneIDi284266.
KEGGihsa:284266.
UCSCiuc002lbl.2. human. [Q6ZMC9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172835 mRNA. Translation: BAD18800.1.
AK290400 mRNA. Translation: BAF83089.1.
AK290402 mRNA. Translation: BAF83091.1.
AK301189 mRNA. Translation: BAG62771.1.
AC087685 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01463.1.
CCDSiCCDS32819.1. [Q6ZMC9-1]
RefSeqiNP_998767.1. NM_213602.2. [Q6ZMC9-1]
UniGeneiHs.287692.

3D structure databases

ProteinModelPortaliQ6ZMC9.
SMRiQ6ZMC9. Positions 44-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000374125.

PTM databases

iPTMnetiQ6ZMC9.
PhosphoSiteiQ6ZMC9.

Polymorphism and mutation databases

BioMutaiSIGLEC15.
DMDMi74762385.

Proteomic databases

PaxDbiQ6ZMC9.
PeptideAtlasiQ6ZMC9.
PRIDEiQ6ZMC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389474; ENSP00000374125; ENSG00000197046. [Q6ZMC9-1]
ENST00000546268; ENSP00000443509; ENSG00000197046. [Q6ZMC9-2]
GeneIDi284266.
KEGGihsa:284266.
UCSCiuc002lbl.2. human. [Q6ZMC9-1]

Organism-specific databases

CTDi284266.
GeneCardsiSIGLEC15.
H-InvDBHIX0019010.
HGNCiHGNC:27596. SIGLEC15.
neXtProtiNX_Q6ZMC9.
PharmGKBiPA162403350.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJR. Eukaryota.
ENOG4111T39. LUCA.
GeneTreeiENSGT00390000011536.
HOGENOMiHOG000013049.
HOVERGENiHBG099550.
InParanoidiQ6ZMC9.
KOiK16352.
OMAiVYLFRFR.
OrthoDBiEOG7TF79D.
PhylomeDBiQ6ZMC9.
TreeFamiTF351096.

Enzyme and pathway databases

ReactomeiR-HSA-2172127. DAP12 interactions.

Miscellaneous databases

GenomeRNAii284266.
PROiQ6ZMC9.

Gene expression databases

BgeeiQ6ZMC9.
CleanExiHS_SIGLEC15.
ExpressionAtlasiQ6ZMC9. baseline and differential.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Spleen and Umbilical cord blood.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Siglec-15: an immune system Siglec conserved throughout vertebrate evolution."
    Angata T., Tabuchi Y., Nakamura K., Nakamura M.
    Glycobiology 17:838-846(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TYROBP AND HCST, MUTAGENESIS OF ARG-143 AND LYS-274.
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSIG15_HUMAN
AccessioniPrimary (citable) accession number: Q6ZMC9
Secondary accession number(s): A8K2Y5, B4DVQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.