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Protein

Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase

Gene

B3GNT6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O(3)-(N-acetyl-alpha-D-galactosaminyl)-L-threonyl/L-seryl-[protein] = UDP + O(3)-(N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-threonyl/L-seryl-[protein].1 Publication

Kineticsi

  1. KM=3.3 mM for GalNAc-alpha-Bn1 Publication
  1. Vmax=4.5 nmol/h/mg enzyme with GalNAc-alpha-Bn as substrate1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • glycoprotein biosynthetic process Source: UniProtKB
  • O-glycan processing Source: Reactome
  • O-glycan processing, core 3 Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.147. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (EC:2.4.1.1471 Publication)
Alternative name(s):
Core 3 synthase
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
Short name:
BGnT-6
Short name:
Beta-1,3-Gn-T6
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 6
Short name:
Beta3Gn-T6
Gene namesi
Name:B3GNT6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:24141. B3GNT6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3119Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini32 – 384353LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164741288.

Chemistry

ChEMBLiCHEMBL2321631.

Polymorphism and mutation databases

DMDMi152033628.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferasePRO_0000289218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6ZMB0.
PeptideAtlasiQ6ZMB0.
PRIDEiQ6ZMB0.

PTM databases

iPTMnetiQ6ZMB0.
PhosphoSiteiQ6ZMB0.

Expressioni

Tissue specificityi

Present in stomach and colon (at protein level). Restricted in the stomach, colon and small intestine, where core 3 structure is present.1 Publication

Inductioni

Down-regulated in gastric and colorectal carcinomas, suggesting that it may be used as a marker for distinguishing between benign adenomas and premalignant lesions (at protein level).

Gene expression databases

BgeeiENSG00000198488.
CleanExiHS_B3GNT6.
ExpressionAtlasiQ6ZMB0. baseline and differential.
GenevisibleiQ6ZMB0. HS.

Organism-specific databases

HPAiHPA012158.
HPA039805.

Structurei

3D structure databases

ProteinModelPortaliQ6ZMB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ6ZMB0.
KOiK00739.
OMAiRFAPYEM.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ6ZMB0.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6ZMB0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFPCRRSLT AKTLACLLVG VSFLALQQWF LQAPRSPREE RSPQEETPEG
60 70 80 90 100
PTDAPAADEP PSELVPGPPC VANASANATA DFEQLPARIQ DFLRYRHCRH
110 120 130 140 150
FPLLWDAPAK CAGGRGVFLL LAVKSAPEHY ERRELIRRTW GQERSYGGRP
160 170 180 190 200
VRRLFLLGTP GPEDEARAER LAELVALEAR EHGDVLQWAF ADTFLNLTLK
210 220 230 240 250
HLHLLDWLAA RCPHARFLLS GDDDVFVHTA NVVRFLQAQP PGRHLFSGQL
260 270 280 290 300
MEGSVPIRDS WSKYFVPPQL FPGSAYPVYC SGGGFLLSGP TARALRAAAR
310 320 330 340 350
HTPLFPIDDA YMGMCLERAG LAPSGHEGIR PFGVQLPGAQ QSSFDPCMYR
360 370 380
ELLLVHRFAP YEMLLMWKAL HSPALSCDRG HRVS
Length:384
Mass (Da):42,748
Last modified:May 29, 2007 - v2
Checksum:iF76C4577D3008429
GO
Isoform 2 (identifier: Q6ZMB0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     25-147: ALQQWFLQAP...RTWGQERSYG → VLQRRRLPPVRPHGPGPARGRPPHPALPHRRRLH
     289-319: Missing.

Note: No experimental confirmation available.
Show »
Length:264
Mass (Da):29,484
Checksum:iEFDAC8A10910D774
GO
Isoform 3 (identifier: Q6ZMB0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     317-335: ERAGLAPSGHEGIRPFGVQ → GARRPGAQRPRGHPGPSACS

Note: Gene prediction based on EST data.
Show »
Length:385
Mass (Da):42,785
Checksum:i18DF2473F7E79C10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641D → N in BAD18819 (PubMed:14702039).Curated
Sequence conflicti191 – 1911A → T in AAH25357 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei25 – 147123ALQQW…ERSYG → VLQRRRLPPVRPHGPGPARG RPPHPALPHRRRLH in isoform 2. 1 PublicationVSP_025963Add
BLAST
Alternative sequencei289 – 31931Missing in isoform 2. 1 PublicationVSP_025964Add
BLAST
Alternative sequencei317 – 33519ERAGL…PFGVQ → GARRPGAQRPRGHPGPSACS in isoform 3. CuratedVSP_047091Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073740 mRNA. Translation: BAB88882.1.
AK127544 mRNA. Translation: BAC87028.1.
AK172863 mRNA. Translation: BAD18819.1.
AP000752 Genomic DNA. No translation available.
BC025357 mRNA. Translation: AAH25357.1.
BC103908 mRNA. Translation: AAI03909.1.
BC103909 mRNA. Translation: AAI03910.1.
BC103910 mRNA. Translation: AAI03911.1.
CCDSiCCDS53681.1. [Q6ZMB0-1]
RefSeqiNP_619651.3. NM_138706.4. [Q6ZMB0-1]
UniGeneiHs.352622.

Genome annotation databases

EnsembliENST00000622824; ENSP00000484640; ENSG00000198488. [Q6ZMB0-1]
GeneIDi192134.
KEGGihsa:192134.
UCSCiuc031xwa.2. human. [Q6ZMB0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073740 mRNA. Translation: BAB88882.1.
AK127544 mRNA. Translation: BAC87028.1.
AK172863 mRNA. Translation: BAD18819.1.
AP000752 Genomic DNA. No translation available.
BC025357 mRNA. Translation: AAH25357.1.
BC103908 mRNA. Translation: AAI03909.1.
BC103909 mRNA. Translation: AAI03910.1.
BC103910 mRNA. Translation: AAI03911.1.
CCDSiCCDS53681.1. [Q6ZMB0-1]
RefSeqiNP_619651.3. NM_138706.4. [Q6ZMB0-1]
UniGeneiHs.352622.

3D structure databases

ProteinModelPortaliQ6ZMB0.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2321631.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

iPTMnetiQ6ZMB0.
PhosphoSiteiQ6ZMB0.

Polymorphism and mutation databases

DMDMi152033628.

Proteomic databases

PaxDbiQ6ZMB0.
PeptideAtlasiQ6ZMB0.
PRIDEiQ6ZMB0.

Protocols and materials databases

DNASUi192134.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000622824; ENSP00000484640; ENSG00000198488. [Q6ZMB0-1]
GeneIDi192134.
KEGGihsa:192134.
UCSCiuc031xwa.2. human. [Q6ZMB0-1]

Organism-specific databases

CTDi192134.
GeneCardsiB3GNT6.
H-InvDBHIX0026120.
HGNCiHGNC:24141. B3GNT6.
HPAiHPA012158.
HPA039805.
MIMi615315. gene.
neXtProtiNX_Q6ZMB0.
PharmGKBiPA164741288.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ6ZMB0.
KOiK00739.
OMAiRFAPYEM.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ6ZMB0.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.147. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii192134.
PROiQ6ZMB0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198488.
CleanExiHS_B3GNT6.
ExpressionAtlasiQ6ZMB0. baseline and differential.
GenevisibleiQ6ZMB0. HS.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN6_HUMAN
AccessioniPrimary (citable) accession number: Q6ZMB0
Secondary accession number(s): E9PKS1
, Q6ZSC5, Q8TAZ4, Q8TDX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Injection into nude mice significantly suppress lung metastasis, indicating that the core structures of O-glycans are profoundly involved in the metastatic capacity of cancer cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.