Reviewed, UniProtKB/Swiss-Prot Q6ZJJ1 (APX4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable L-ascorbate peroxidase 4
    EC=1.11.1.11
Alternative name(s):
    OsAPx04

Gene names

Name:	APX4
Ordered Locus Names:	Os08g0549100, LOC_Os08g43560
ORF Names:	OJ1479_B11.9

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in hydrogen peroxide removal By similarity.
Catalytic activity	L-ascorbate + H(2)O(2) = dehydroascorbate + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group By similarity. Binds 1 potassium or calcium ion per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Sequence similarities	Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
Caution	According to Ref.3, it may be peroxisomal. There is however no experimental evidence to prove this.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Membrane
Domain	Transmembrane
Ligand	Calcium Heme Iron Metal-binding Potassium
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Probable L-ascorbate peroxidase 4		PRO_0000055596
Regions
	Transmembrane	263 – 283	21	Potential
Sites
	Active site	40	1	Proton acceptor By similarity
	Metal binding	160	1	Iron (heme axial ligand) By similarity
	Metal binding	161	1	Potassium or calcium By similarity
	Metal binding	177	1	Potassium or calcium By similarity
	Metal binding	184	1	Potassium or calcium By similarity
	Site	36	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZJJ1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5B605C442EB57807	FASTA	291	31,738
	10 20 30 40 50 60 MAAPVVDAEY LRQVDRARRH LRALISSKGC APIMLRLAWH DAGTYDVNTK TGGANGSIRY 70 80 90 100 110 120 EEEYTHGSNA GLKIAIDLLE PIKAKSPKIT YADLYQLAGV VAVEVTGGPT VEFIPGRRDS 130 140 150 160 170 180 SVCPREGRLP DAKKGALHLR DIFYRMGLSD KDIVALSGGH TLGRAHPERS GFEGAWTQEP 190 200 210 220 230 240 LKFDNSYFLE LLKGESEGLL KLPTDKALLE DPSFRRYVDL YARDEDTFFK DYAESHKKLS 250 260 270 280 290 ELGFTPRSSG PASTKSDLST GAVLAQSAVG VAVAAAVVIV SYLYEASKKS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oryza sativa nipponbare chromosome 8 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[3]	"Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome." Teixeira F.K., Menezes-Benavente L., Margis R., Margis-Pinheiro M. J. Mol. Evol. 59:761-770(2004) [PubMed: 15599508] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003912 Genomic DNA. Translation: BAD08951.1. AK070842 mRNA. No translation available.
RefSeq	NP_001062439.1.
UniGene	Os.10609
3D structure databases
HSSP	HSSP built from PDB template 1APX based on UniProtKB P48534.
ModBase	Search...
Protein family/group databases
PeroxiBase	1868. OsAPx04.
Genome annotation databases
GeneID	4346247.
KEGG	osa:4346247.
Organism-specific databases
Gramene	Q6ZJJ1.
Family and domain databases
InterPro	IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00459. ASPEROXIDASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX4_ORYSJ

Accession

Primary (citable) accession number: Q6ZJJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)