Q6ZIX2 (SMT1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cycloartenol-C-24-methyltransferase 1
EC=2.1.1.41

Alternative name(s):
24-sterol C-methyltransferase 1
Short name=Sterol C-methyltransferase 1

Gene names

Name:	Smt1-1
Ordered Locus Names:	Os07g0206700, LOC_Os07g10600
ORF Names:	OJ1119_B04.19-1

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryo development ending in seed dormancy Inferred from electronic annotation. Source: EnsemblPlants/Gramene sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Cycloartenol-C-24-methyltransferase 1		PRO_0000124803
Experimental info
	Sequence conflict	299	1	Y → YKALEY in AAC34988. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6ZIX2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1510C2F576B47ADB	FASTA	344	38,612
	10 20 30 40 50 60 MSRSGAMDLA SGLGGKITKD EVKSAVDEYE KYHGYYGGKE EARKSNYTDM VNKYYDLATS 70 80 90 100 110 120 FYEYGWGESF HFAHRWNGES LRESIKRHEH FLALQLGVKP GMKVLDVGCG IGGPLREIAK 130 140 150 160 170 180 FSLASVTGLN NNEYQITRGK ELNRVAGVSG TCDFVKADFM KMPFSDNTFD AVYAIEATCH 190 200 210 220 230 240 APDPVGCYKE IYRVLKPGQC FAVYEWCITD HYEPNNATHK RIKDEIELGN GLPDIRSTQQ 250 260 270 280 290 300 CLQAAKDAGF EVIWDKDLAE DSPVPWYLPL DPSRFSLSSF RLTTVGRAIT RTMVKALEYV 310 320 330 340 GLAPQGSERV SNFLEKAAEG LVEGGKKEIF TPMYFFLVRK PISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis." Bouvier-Nave P., Husselstein T., Benveniste P. Eur. J. Biochem. 256:88-96(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Root.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042332 mRNA. Translation: AAC34988.1. AP003943 Genomic DNA. Translation: BAC83238.1.
RefSeq	NP_001059154.1. NM_001065689.1.
UniGene	Os.2224.
3D structure databases
ProteinModelPortal	Q6ZIX2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZIX2. 2 interactions.
Proteomic databases
PRIDE	Q6ZIX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g10600.2; LOC_Os07g10600.2; LOC_Os07g10600.
GeneID	4342690.
KEGG	dosa:Os07t0206700-01. osa:4342690.
Organism-specific databases
Gramene	Q6ZIX2.
Phylogenomic databases
eggNOG	COG0500.
KO	K00559.
ProtClustDB	CLSN2687038.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-7706.
UniPathway	UPA00766.
Gene expression databases
ArrayExpress	Q6ZIX2.
Family and domain databases
InterPro	IPR025810. ERG6. IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMT1_ORYSJ

Accession

Primary (citable) accession number: Q6ZIX2
Secondary accession number(s): O82426

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program