Reviewed, UniProtKB/Swiss-Prot Q6ZIX2 (SMT1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cycloartenol-C-24-methyltransferase 1
    EC=2.1.1.41
Alternative name(s):
    24-sterol C-methyltransferase 1
      Short name=Sterol C-methyltransferase 1

Gene names

Name:	Smt1-1
Ordered Locus Names:	Os07g0206700, LOC_Os07g10600
ORF Names:	OJ1119_B04.19-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Cycloartenol-C-24-methyltransferase 1		PRO_0000124803
Experimental info
	Sequence conflict	299	1	Y → YKALEY Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6ZIX2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1510C2F576B47ADB	FASTA	344	38,612
	10 20 30 40 50 60 MSRSGAMDLA SGLGGKITKD EVKSAVDEYE KYHGYYGGKE EARKSNYTDM VNKYYDLATS 70 80 90 100 110 120 FYEYGWGESF HFAHRWNGES LRESIKRHEH FLALQLGVKP GMKVLDVGCG IGGPLREIAK 130 140 150 160 170 180 FSLASVTGLN NNEYQITRGK ELNRVAGVSG TCDFVKADFM KMPFSDNTFD AVYAIEATCH 190 200 210 220 230 240 APDPVGCYKE IYRVLKPGQC FAVYEWCITD HYEPNNATHK RIKDEIELGN GLPDIRSTQQ 250 260 270 280 290 300 CLQAAKDAGF EVIWDKDLAE DSPVPWYLPL DPSRFSLSSF RLTTVGRAIT RTMVKALEYV 310 320 330 340 GLAPQGSERV SNFLEKAAEG LVEGGKKEIF TPMYFFLVRK PISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis." Bouvier-Nave P., Husselstein T., Benveniste P. Eur. J. Biochem. 256:88-96(1998) [PubMed: 9746350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Root.
[2]	"Oryza sativa nipponbare chromosome 7 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF042332 mRNA. Translation: AAC34988.1. AP003943 Genomic DNA. Translation: BAC83238.1.
RefSeq	NP_001059154.1.
UniGene	Os.2224
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6ZIX2. 2 interactions.
Genome annotation databases
GeneID	4342690.
KEGG	osa:4342690.
Organism-specific databases
Gramene	Q6ZIX2.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-7706.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMT1_ORYSJ

Accession

Primary (citable) accession number: Q6ZIX2
Secondary accession number(s): O82426

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2004
Last modified:	May 5, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)