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Protein

Cycloartenol-C-24-methyltransferase 1

Gene

Smt1-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.By similarity

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway:isterol biosynthesis

This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7706.
UniPathwayiUPA00766.

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol-C-24-methyltransferase 1 (EC:2.1.1.41)
Alternative name(s):
24-sterol C-methyltransferase 1
Short name:
Sterol C-methyltransferase 1
Gene namesi
Name:Smt1-1
Ordered Locus Names:Os07g0206700, LOC_Os07g10600
ORF Names:OJ1119_B04.19-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 7

Organism-specific databases

GrameneiQ6ZIX2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Cycloartenol-C-24-methyltransferase 1PRO_0000124803Add
BLAST

Proteomic databases

PRIDEiQ6ZIX2.

Expressioni

Gene expression databases

ExpressionAtlasiQ6ZIX2. baseline.

Interactioni

Protein-protein interaction databases

IntActiQ6ZIX2. 2 interactions.
STRINGi39947.LOC_Os07g10600.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ZIX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0500.
InParanoidiQ6ZIX2.
KOiK00559.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ZIX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSGAMDLA SGLGGKITKD EVKSAVDEYE KYHGYYGGKE EARKSNYTDM
60 70 80 90 100
VNKYYDLATS FYEYGWGESF HFAHRWNGES LRESIKRHEH FLALQLGVKP
110 120 130 140 150
GMKVLDVGCG IGGPLREIAK FSLASVTGLN NNEYQITRGK ELNRVAGVSG
160 170 180 190 200
TCDFVKADFM KMPFSDNTFD AVYAIEATCH APDPVGCYKE IYRVLKPGQC
210 220 230 240 250
FAVYEWCITD HYEPNNATHK RIKDEIELGN GLPDIRSTQQ CLQAAKDAGF
260 270 280 290 300
EVIWDKDLAE DSPVPWYLPL DPSRFSLSSF RLTTVGRAIT RTMVKALEYV
310 320 330 340
GLAPQGSERV SNFLEKAAEG LVEGGKKEIF TPMYFFLVRK PISE
Length:344
Mass (Da):38,612
Last modified:July 5, 2004 - v1
Checksum:i1510C2F576B47ADB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991Y → YKALEY in AAC34988 (PubMed:9746350).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042332 mRNA. Translation: AAC34988.1.
AP003943 Genomic DNA. Translation: BAC83238.1.
RefSeqiNP_001059154.1. NM_001065689.1.
UniGeneiOs.2224.

Genome annotation databases

EnsemblPlantsiOS07T0206700-01; OS07T0206700-01; OS07G0206700.
GeneIDi4342690.
KEGGiosa:4342690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042332 mRNA. Translation: AAC34988.1.
AP003943 Genomic DNA. Translation: BAC83238.1.
RefSeqiNP_001059154.1. NM_001065689.1.
UniGeneiOs.2224.

3D structure databases

ProteinModelPortaliQ6ZIX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6ZIX2. 2 interactions.
STRINGi39947.LOC_Os07g10600.1.

Proteomic databases

PRIDEiQ6ZIX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS07T0206700-01; OS07T0206700-01; OS07G0206700.
GeneIDi4342690.
KEGGiosa:4342690.

Organism-specific databases

GrameneiQ6ZIX2.

Phylogenomic databases

eggNOGiCOG0500.
InParanoidiQ6ZIX2.
KOiK00559.

Enzyme and pathway databases

UniPathwayiUPA00766.
BioCyciMetaCyc:MONOMER-7706.

Gene expression databases

ExpressionAtlasiQ6ZIX2. baseline.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis."
    Bouvier-Nave P., Husselstein T., Benveniste P.
    Eur. J. Biochem. 256:88-96(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Root.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiSMT1_ORYSJ
AccessioniPrimary (citable) accession number: Q6ZIX2
Secondary accession number(s): O82426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.