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Protein

Probable sucrose-phosphate synthase 4

Gene

SPS4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase 5 (SPS5), Probable sucrose-phosphate synthase 4 (SPS4), Probable sucrose-phosphate synthase 3 (SPS3), Probable sucrose-phosphate synthase 1 (SPS1), Probable sucrose-phosphate synthase 2 (SPS2)
  2. Probable sucrose-phosphatase 3 (SPP3), Probable sucrose-phosphatase 1 (SPP1), Sucrose-phosphatase 2 (SPP2)
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.14. 4460.
UniPathwayiUPA00371; UER00545.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sucrose-phosphate synthase 4 (EC:2.4.1.14)
Alternative name(s):
Sucrose phosphate synthase 4F
Short name:
OsSPS4F
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS4
Synonyms:SPS8
Ordered Locus Names:Os08g0301500, LOC_Os08g20660
ORF Names:OJ1115_A07.105
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 8, cultivar: Nipponbare

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Probable sucrose-phosphate synthase 4PRO_0000413643Add
BLAST

Proteomic databases

PaxDbiQ6ZHZ1.
PRIDEiQ6ZHZ1.

PTM databases

iPTMnetiQ6ZHZ1.

Expressioni

Tissue specificityi

Expressed in germinating seeds.1 Publication

Developmental stagei

Expressed in source leaves and sink leaves.1 Publication

Inductioni

Circadian-regulated, with the highest expression 1 hour after the beginning of dark period (in 14 hours light/10 hours dark cycle).1 Publication

Gene expression databases

GenevisibleiQ6ZHZ1. OS.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os08g20660.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ZHZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
HOGENOMiHOG000009685.
InParanoidiQ6ZHZ1.
KOiK00696.
OMAiKTWVRTA.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6ZHZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNDWINSY LEAILDAGGA AGEISAAAGG GGDGAAATGE KRDKSSLMLR
60 70 80 90 100
ERGRFSPARY FVEEVISGFD ETDLYKTWVR TAAMRSPQER NTRLENMSWR
110 120 130 140 150
IWNLARKKKQ IEGEEASRLA KQRLEREKAR RYAAADMSED LSEGEKGENI
160 170 180 190 200
NESSSTHDES TRGRMPRIGS TDAIEAWASQ HKDKKLYIVL ISIHGLIRGE
210 220 230 240 250
NMELGRDSDT GGQVKYVVEL ARALGSTPGV YRVDLLTRQI SAPDVDWSYG
260 270 280 290 300
EPTEMLSPRN SENFGHDMGE SSGAYIVRIP FGPRDKYIPK EHLWPHIQEF
310 320 330 340 350
VDGALVHIMQ MSKVLGEQVG SGQLVWPVVI HGHYADAGDS AALLSGALNV
360 370 380 390 400
PMIFTGHSLG RDKLEQLLKQ GRQTRDEINT IYKIMRRIEA EELCLDASEI
410 420 430 440 450
IITSTRQEIE QQWGLYDGFD LTMARKLRAR IKRGVSCYGR YMPRMIAVPP
460 470 480 490 500
GMEFSHIVPH DVDQDGEEAN EDGSGSTDPP IWADIMRFFS NPRKPMILAL
510 520 530 540 550
ARPDPKKNIT TLVKAFGEHR ELRNLANLTL IMGNRDVIDE MSSTNSAVLT
560 570 580 590 600
SILKLIDKYD LYGQVAYPKH HKQSEVPDIY RLAARTKGVF INCAFIEPFG
610 620 630 640 650
LTLIEAAAYG LPMVATRNGG PVDIHRVLDN GILVDPHNQN EIAEALYKLV
660 670 680 690 700
SDKQLWAQCR QNGLKNIHQF SWPEHCKNYL SRVGTLKPRH PRWQKSDDAT
710 720 730 740 750
EVSEADSPGD SLRDVHDISL NLKLSLDSEK SSTKENSVRR NLEDAVQKLS
760 770 780 790 800
RGVSANRKTE SVENMEATTG NKWPSLRRRK HIVVIAIDSV QDANLVEIIK
810 820 830 840 850
NIFVASSNER LSGSVGFVLS TSRAISEVHS LLTSGGIEAT DFDAFICNSG
860 870 880 890 900
SDLCYPSSNS EDMLSPAELP FMIDLDYHTQ IEYRWGGEGL RKTLICWAAE
910 920 930 940 950
KSEGGQVVLV EDEECSSTYC ISFRVKNAEA VPPVKELRKT MRIQALRCHV
960 970 980 990 1000
LYSHDGSKLN VIPVLASRSQ ALRYLYIRWG VELSNMTVVV GESGDTDYEG
1010 1020 1030 1040 1050
LLGGVHKTII LKGSFNAVPN QVHAARSYSL QDVISFDKPG ITSIEGYGPD
1060
NLKSALQQFG ILKDNV
Length:1,066
Mass (Da):118,725
Last modified:July 5, 2004 - v1
Checksum:iAB4AE780FEA070A2
GO

Sequence cautioni

The sequence AAQ56529.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti527 – 5271N → S in AK101676 (PubMed:12869764).Curated
Sequence conflicti615 – 6151A → T in AK101676 (PubMed:12869764).Curated
Sequence conflicti900 – 9001E → G in AK101676 (PubMed:12869764).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY360393 Genomic DNA. Translation: AAQ56529.1. Sequence problems.
AP004041 Genomic DNA. Translation: BAC92378.1.
AP008214 Genomic DNA. Translation: BAF23409.1.
AP014964 Genomic DNA. Translation: BAT04814.1.
AK101676 mRNA. No translation available.
RefSeqiXP_015650062.1. XM_015794576.1.
XP_015650063.1. XM_015794577.1.
UniGeneiOs.100489.

Genome annotation databases

EnsemblPlantsiOS08T0301500-01; OS08T0301500-01; OS08G0301500.
GeneIDi4345220.
GrameneiOS08T0301500-01; OS08T0301500-01; OS08G0301500.
KEGGiosa:4345220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY360393 Genomic DNA. Translation: AAQ56529.1. Sequence problems.
AP004041 Genomic DNA. Translation: BAC92378.1.
AP008214 Genomic DNA. Translation: BAF23409.1.
AP014964 Genomic DNA. Translation: BAT04814.1.
AK101676 mRNA. No translation available.
RefSeqiXP_015650062.1. XM_015794576.1.
XP_015650063.1. XM_015794577.1.
UniGeneiOs.100489.

3D structure databases

ProteinModelPortaliQ6ZHZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g20660.1.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

PTM databases

iPTMnetiQ6ZHZ1.

Proteomic databases

PaxDbiQ6ZHZ1.
PRIDEiQ6ZHZ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0301500-01; OS08T0301500-01; OS08G0301500.
GeneIDi4345220.
GrameneiOS08T0301500-01; OS08T0301500-01; OS08G0301500.
KEGGiosa:4345220.

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
HOGENOMiHOG000009685.
InParanoidiQ6ZHZ1.
KOiK00696.
OMAiKTWVRTA.

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545.
BRENDAi2.4.1.14. 4460.

Gene expression databases

GenevisibleiQ6ZHZ1. OS.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants."
    Lutfiyya L.L., Xu N., D'Ordine R.L., Morrell J.A., Miller P.W., Duff S.M.
    J. Plant Physiol. 164:923-933(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "Tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family in rice."
    Okamura M., Aoki N., Hirose T., Yonekura M., Ohto C., Ohsugi R.
    Plant Sci. 181:159-166(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.

Entry informationi

Entry nameiSPSA4_ORYSJ
AccessioniPrimary (citable) accession number: Q6ZHZ1
Secondary accession number(s): A0A0P0XEF8, Q6UU32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.