Cinnamyl alcohol dehydrogenase 2 - Oryza sativa subsp. japonica (Rice)

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Q6ZHS4 (CADH2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cinnamyl alcohol dehydrogenase 2
Short name=OsCAD2
EC=1.1.1.195

Alternative name(s):
Protein GOLD HULL AND INTERNODE 2

Gene names

Name:	CAD2
Synonyms:	CAD, GH2
Ordered Locus Names:	Os02g0187800, LOC_Os02g09490
ORF Names:	OJ1073_F05.31, OJ1145_F01.6

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in lignin biosynthesis. Catalyzes the final step specific for the production of lignin monomers. Catalyzes the NADPH-dependent reduction of coniferaldehyde and sinapaldehyde to their respective alcohols. Ref.6
Catalytic activity	Cinnamyl alcohol + NADP⁺ = cinnamaldehyde + NADPH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Tissue specificity	Expressed in roots behind the root tips in the pericycle region and layer of cortical cells adjacent to the exodermis. Expressed in vascular bundles and lateral veins of leaf sheaths and blades. Expressed in the vicinity of vascular bundles in the first internode below the inflorescence. Ref.5 Ref.6
Disruption phenotype	Reduced lignin content, reddish-brown coloration of panicles and internodes, and golden yellow seeds. Ref.6
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.
Biophysicochemical properties	Kinetic parameters: K_M=4.4 µM for coniferaldehyde Ref.6 K_M=20.8 µM for sinapaldehyde V_max=32.9 pmol/sec/µg enzyme with coniferaldehyde as substrate V_max=87.0 pmol/sec/µg enzyme with sinapaldehyde as substrate

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	Metal-binding NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lignin biosynthetic process Inferred from direct assay Ref.5 Ref.6. Source: Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	cinnamyl-alcohol dehydrogenase activity Inferred from direct assay Ref.6. Source: Gramene nucleotide binding Inferred from electronic annotation. Source: InterPro sinapyl alcohol dehydrogenase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 363

363

Cinnamyl alcohol dehydrogenase 2

PRO_0000382641

Regions

Nucleotide binding

188 – 193

NADP By similarity

Nucleotide binding

211 – 216

NADP By similarity

Nucleotide binding

298 – 300

NADP By similarity

Sites

Metal binding

Zinc 1; catalytic By similarity

Metal binding

Zinc 1; catalytic By similarity

Metal binding

Zinc 1; catalytic By similarity

Metal binding

100

Zinc 2 By similarity

Metal binding

103

Zinc 2 By similarity

Metal binding

106

Zinc 2 By similarity

Metal binding

114

Zinc 2 By similarity

Metal binding

163

Zinc 1; catalytic By similarity

Binding site

NADP By similarity

Binding site

167

NADP By similarity

Binding site

251

NADP By similarity

Binding site

275

NADP; via carbonyl oxygen By similarity

Experimental info

Mutagenesis

185

G → D: Loss of activity. Ref.6

Sequence conflict

293

K → E in AK105011. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q6ZHS4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: EFD21231AFB35B4B
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FASTA

363

38,644

        10         20         30         40         50         60 
MGSLAAEKTV TGWAARDASG HLTPYNYTLR KTGPEDVVVK VLYCGICHTD IHQAKNHLGA 

        70         80         90        100        110        120 
SKYPMVPGHE VVGEVVEVGP EVTKYSAGDV VGVGVIVGCC RECHPCKANV EQYCNKRIWS 

       130        140        150        160        170        180 
YNDVYTDGRP TQGGFASAMV VDQKFVVKIP AGLAPEQAAP LLCAGLTVYS PLKHFGLMSP 

       190        200        210        220        230        240 
GLRGGVLGLG GVGHMGVKVA KSMGHHVTVI SSSARKRGEA MDDLGADAYL VSSDAAAMAA 

       250        260        270        280        290        300 
AGDSLDYIID TVPVHHPLEP YLALLKLDGK LILMGVINQP LSFISPMVML GRKAITGSFI 

       310        320        330        340        350        360 
GSMAETEEVL NFCVDKGLTS QIEVVKMDYV NQALERLERN DVRYRFVVDV AGSNIDDADA 


PPA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"The cDNA encoding cinnamyl alcohol dehydrogenase in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 206-363. Strain: cv. Fujiminori.
[5]	"Structure of the cinnamyl-alcohol dehydrogenase gene family in rice and promoter activity of a member associated with lignification." Tobias C.M., Chow E.K. Planta 220:678-688(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[6]	"GOLD HULL AND INTERNODE2 encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase in rice." Zhang K., Qian Q., Huang Z., Wang Y., Li M., Hong L., Zeng D., Gu M., Chu C., Cheng Z. Plant Physiol. 140:972-983(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-185.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003990 Genomic DNA. Translation: BAD15428.1. AP004046 Genomic DNA. Translation: BAD15519.1. AP008208 Genomic DNA. Translation: BAF08046.1. AK105011 mRNA. No translation available. AB122054 mRNA. Translation: BAD14921.1. BK003969 Genomic DNA. Translation: DAA02237.1.
RefSeq	NP_001046132.1. NM_001052667.1.
UniGene	Os.8684.
3D structure databases
HSSP	HSSP built from PDB template 1YQD based on UniProtKB Q94G59.
ProteinModelPortal	Q6ZHS4.
ModBase	Search...
Proteomic databases
PRIDE	Q6ZHS4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g09490.1; LOC_Os02g09490.1; LOC_Os02g09490.
GeneID	4328552.
KEGG	osa:4328552.
Organism-specific databases
Gramene	Q6ZHS4.
Phylogenomic databases
eggNOG	COG1064.
KO	K00083.
OMA	ANVEQYC.
ProtClustDB	PLN02514.
Enzyme and pathway databases
SABIO-RK	Q6ZHS4.
UniPathway	UPA00711.
Gene expression databases
ArrayExpress	Q6ZHS4.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CADH2_ORYSJ

Accession

Primary (citable) accession number: Q6ZHS4
Secondary accession number(s): Q75W59

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents