Reviewed, UniProtKB/Swiss-Prot Q6ZGU9 (EXPA5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Expansin-A5
Alternative name(s):
    OsEXPA5
    Alpha-expansin-5
    OsEXP5
    OsaEXPa1.20

Gene names

Name:	EXPA5
Synonyms:	EXP5
Ordered Locus Names:	Os02g0744200, LOC_Os02g51040
ORF Names:	OJ1118_G04.3, OJ1734_E02.30

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. May be required for rapid internodal elongation in deepwater rice during submergence By similarity.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in panicles and flowers. Ref.4
Developmental stage	Expressed in the growing regions of roots, coleoptiles, internodes and leaves. Ref.1
Induction	By gibberellin (GA3) and wounding. Ref.1
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 291	269	Expansin-A5		PRO_0000251984
Regions
	Domain	82 – 197	116	Expansin-like EG45
	Domain	207 – 287	81	Expansin-like CBD
	Compositional bias	24 – 127	104	Gly-rich
Amino acid modifications
	Disulfide bond	85 ↔ 113		By similarity
	Disulfide bond	116 ↔ 192		By similarity
	Disulfide bond	121 ↔ 131		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZGU9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 53D7DC2DAE65F94C	FASTA	291	30,175
	10 20 30 40 50 60 MSSRRDVLAV VLVAALLPPA LSRGLWLGHH GLGHGHGRWR APHVGGHGQG QGPQQHAPLG 70 80 90 100 110 120 GGGWSSAHAT FYGGGDASGT MGGACGYGNL YSQGYGTNTA ALSTALFNNG LSCGACFEVR 130 140 150 160 170 180 CDAGGGGSHS CLPGSVVVTA TNFCPPNNAL PSDDGGWCNP PRAHFDMSQP VFQRIALFKA 190 200 210 220 230 240 GIVPVSYRRV ACQKKGGIRF TINGHSYFNL VLVTNVGGAG DVHAVAVKSE RSAAWQALSR 250 260 270 280 290 NWGQNWQSAA LLDGQALSFR VTTGDGRSVV SNNAVPRGWS FGQTFSGAQF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of alpha-expansin and expansin-like genes in deepwater rice." Lee Y., Kende H. Plant Physiol. 130:1396-1405(2002) [PubMed: 12428004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE, INDUCTION.
[2]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.
[4]	"Characterization and transcriptional expression of the alpha-expansin gene family in rice." Shin J.-H., Jeong D.-H., Park M.C., An G. Mol. Cells 20:210-218(2005) [PubMed: 16267395] [Abstract] Cited for: TISSUE SPECIFICITY.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF247162 mRNA. Translation: AAF62180.1. AF394546 Genomic DNA. Translation: AAL24482.1. AP004114 Genomic DNA. Translation: BAD15536.1. AP005297 Genomic DNA. Translation: BAD16120.1.
RefSeq	NP_001048101.1.
UniGene	Os.5022
3D structure databases
HSSP	HSSP built from PDB template 1N10 based on UniProtKB P43213.
SMR	Q6ZGU9. Positions 60-286.
ModBase	Search...
Genome annotation databases
GeneID	4330706.
KEGG	osa:4330706.
Organism-specific databases
Gramene	Q6ZGU9.
Family and domain databases
InterPro	IPR009009. Barwin-related_endoglucanase. IPR007112. Expan_endogl. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR014734. Pollen_allergen. IPR005132. RlpA. [Graphical view]
Gene3D	G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit. G3DSA:2.40.40.40. Pollen_allergen. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXPA5_ORYSJ

Accession

Primary (citable) accession number: Q6ZGU9
Secondary accession number(s): Q9M4X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)